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| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | 地理集聚、行业集中与中国企业出口模式的差异性显示文摘基于2000~2006年间持续经营的47000多家制造业企业数据,本文经验考察了外资参与、地理集聚与出口外溢对我国企业出口模式选择的影响。将全部企业划分为非出口、持续出口、新增出口以及间断出口四种不同类型,本文借鉴显性比较优势(RCA)思想构造了区域内与区域间、产业内与产业间外溢指标。多项式分布logit模型的估计结果表明,外资进入不仅显著地促进了企业自身的出口可能性,而且还通过对内资企业的出口外溢效应形成了企业出口的地理扩散与行业集聚效果。但与盛行的“本地化外溢”假说相反,我们发现出口外溢效果并未表现出随地理距离缩小而更为强烈的本地化现象,即局部地区的大量同质性出口企业过度集聚导致了我国企业出口外溢的“去本地化”效应,而且这一结果在控制了外溢指标的可能相关性、外资来源地以及企业所有制之后仍然稳健。 | 包群 邵敏 Ligang Song | 2012 | 管理世界2012，28，9： | 33 |
| 3 | Enhanced and irreversible sorption of pesticide pyrimethanil by soil amended with biochars显示文摘Biochar derived from partial combustion of vegetation is ubiquitous and potentially effective in sequestration of environmental contaminants.Biochars were prepared by burning of red gum(Eucalyptus spp.) woodchips at 450 and 850°C(labeled as BC450 and BC850).These two biochars were found to possess markedly different properties in terms of surface area and porosity.Short-term equilibration tests(24 hr) were conducted to assess the sorption-desorption behavior of pyrimethanil in the soil amended with various amounts of biochar of each type,with a special focus on the desorption behavior of the sorbed pesticide through four times successive desorption by dilution.Sorption coefficient and isotherm nonlinearity of the amended soils progressively increased with the content of biochar in the soil.Biochar BC850 with higher surface area and microporosity showed a stronger effect on the reversibility of sorption pesticide.The soils amended with 5% BC450 and 1% BC850 had nearly the same sorption capacity for pyrimethanil;however,their desorption processes were very different with 13.65% and 1.49% of the sorbed pesticide being released,respectively.This study suggested that biochar in soil could be an important factor for immobilization of a pesticide and thus affecting its environment fate in soil. | Xiangyang Yu Ligang Pan Guangguo Ying Rai S. Kookana | 2010 | Journal of Environmental Sciences2010，22，4： | 19 |
| 4 | Nanotwinned and hierarchical nanotwinned metals:a review of experimental,computational and theoretical efforts显示文摘The recent studies on nanotwinned(NT)and hierarchical nanotwinned(HNT)face-centered cubic(FCC)metals are presented in this review.The HNT structures have been supposed as a kind of novel structure to bring about higher strength/ductility than NT counterparts in crystalline materials.We primarily focus on the recent developments of the experimental,atomistic and theoretical studies on the NT and HNT structures in the metallic materials.Some advanced bottom-up and top-down techniques for the fabrication of NT and HNT structures are introduced.The deformation induced HNT structures are available by virtue of severe plastic deformation(SPD)based techniques while the synthesis of growth HNT structures is so far almost unavailable.In addition,some representative molecular dynamics(MD)studies on the NT and HNT FCC metals unveil that the nanoscale effects such as twin spacing,grain size and plastic anisotropy greatly alter the performance of NT and HNT metals.The HNT structures may initiate unique phenomena in comparison with the NT ones.Furthermore,based on the phenomena and mechanisms revealed by experimental and MD simulation observations,a series of theoretical models have been proposed.They are effective to describe the mechanical behaviors of NT and HNT metals within the applicable scope.So far the development of manufacturing technologies of HNT structures,as well as the studies on the effects of HNT structures on the properties of metals are still in its infancy.Further exploration is required to promote the design of advanced materials. | Ligang Sun Xiaoqiao He Jian Lu | 2018 | npj Computational Materials2018，，1： | 14 |
| 5 | Fluidization of non-spherical particles:Sphericity,Zingg factor and other fluidization parameters显示文摘A comparison of sphericity and Zingg factor for particle morphology and description of fluidized-bed dynamics are presented.It is found that Zingg factor Fz = LH/B2(where L,H and B are,respectively,the length,breadth and height of a particle) well describes the effect of particle morphology.Experimental results show that non-spherical particles give poor fluidizing quality as compared to spherical particles in terms of pressure drop,Umf,etc.With the same volume-equivalent diameter,non-spherical particles have lower Umf and fluidizing coefficient δ.Some smooth curves have been obtained between the parameters δ,Umf and Fz.The quality of fluidization could be evaluated by fluidizing coefficient,which has been correlated to the Zingg factor and minimum fluidizing velocity in this paper. | Baiqian Liu Xiaohui Zhang Ligang Wang Hui Hong | 2008 | Particuology2008，6，2： | 14 |
| 6 | Motion behavior of particles in air-solid magnetically stabilized fluidized beds for separation显示文摘In order to study the settling mechanism of particles in an air-solid magnetically stabilized fluidized bed(MSFB) for separation,we carried out free settling and quasi-zero settling tests on the tracing particles.The results show that the main resistance forces as the tracing particles settled in an air-solid MSFB were motion resistance force and yield force.The motion resistance and yield forces greatly hindered the free settling of the particles by greatly decreasing the acceleration for settling process of the particles.The acceleration decreased from 3022.62 cm/s 2 to zero in 0.1 s,and in the end,the particles stopped in the air-solid MSFB.The yield force on particles increased with increasing the magnetic field intensity,resulting in decrease of the quasi-zero settling displacement.However,the yield force on particles decreased with increasing the fluidized air velocity,leading to increase of the quasi-zero settling displacement.When the structure and operating parameters of the air-solid MSFB were set up,the yield stress on particles stopped in an air-solid MSFB was a function of diameter and density of particles.The settling displacements of equal diameter particles increased with increasing their densities,and the settling displacements of equal density particles increased with increasing their diameters. | Song Shulei a,,Zhao Yuemin a,Luo Zhenfu a,Tang Ligang b a School of Chemical Engineering and Technology,China University of Mining & Technology,Xuzhou 221116,China b Coal Mining and Designing Department,Tiandi Science & Technology Company Co.Ltd.,Beijing 100013,China | 2012 | International Journal of Mining Science and Technology2012，22，5： | 13 |
| 7 | State-owned Enterprises' Outward Investment and the Structural Reform in China显示文摘自从中国在 2001 的 WTO 的就职,中国以从事向外直接的投资在一条陡峭的学习曲线上,并且国有企业(SOEs ) 在这个运动起了一个占优势的作用。我们主张根据它国内改革上的影响被担心,由 SOEs 海外的那项投资是一把双重的剑。投资海外提议机会在中国,而是如此的投资加深结构的改革能也加强一些 SOEs 的垄断位置,它与国内改革的目的不一致。因此,中国关于竞争,所有权和规定加深国内改革是重要的,到从海外投资最大化好处。现在的纸也讨论大楼市场兼容机机构怎么将导致增加的革新。这提供机会让中国公司有效地赶上先进技术在海外市场仍然保持竞争。 | Ligang Song Jidong Yang Yongsheng Zhang | 2011 | China & World Economy2011，19，4： | 13 |
| 8 | Ecological Planning for Sino-Singapore Ecological Science and Technology Town of Suzhou显示文摘1. Background The construction of Sino-Singapore Ecological Science and Technology Town (SSESTT), which is a part of Suzhou Industrial Park, was initiated in 2005 under the circumstance of rapid | LIN Borong, Associate Professor, School of Architecture, Tsinghua University, Beijing, P. R. China. QIN Youguo, Professor, School of Architecture, Tsinghua University, Beijing, P. R. China. LIU Ligang, PhD Candidate, School of Architecture, Tsinghua University, Beijing, P. R. China. SONG Yehao, Associate Professor, School of Architecture, Tsinghua University, Beijing, P. R. China. | 2008 | China City Planning Review2008，17，4： | 12 |
| 9 | China as a Global Manufacturing Powerhouse:Strategic Considerations and Structural Adjustment显示文摘这研究通过带制造的开发检验中国的上升的性质和后果到世界经济事情的中心。我们中国除了在它它的生长可能相当被期望变慢的发展过程的点仍然好的历史的分析表演,或精力,资源和后退的生长的碳紧张。当中国变得更活跃地从事对放松全球不平衡的背景在家和国外处理结构的问题时,学习主张工业化的当前的轨道将不得不被改变。中国可能采用的一有利策略将是接近最后提高了它到它全球卓越的位置的 USA 的不可思议地多产的畅销集成努力。在中国重工业的过量能力的轮转的再度出现,关于另外的主要区域的中等术语能力的严肃的问题在中国市场份额推进大获得提供,并且在工业开发的当代的风格和生物圈的健康之间的空冲突强烈显示那现在是时间催化将支撑的要求的调整和改革进程持续长期间繁荣。 | Huw McKay Ligang Song | 2010 | China & World Economy2010，18，1： | 12 |
| 10 | Itinerant-electron metamagnetic transition and giant magnetic entropy change in La_(0.8)Ce_(0.2)Fe_(11.4)Si_(1.6) compound显示文摘The crystal structure, itinerant-electron metamagnetic transition (IEMT) and magnetocaloric effect (MCE) in the iron-based rare-earth intermetallic compound La0.8Ce0.2Fe11.4Si1.6 have been investi- gated. The powder X-ray diffraction revealed that the ingot of La0.8Ce0.2Fe11.4Si1.6 annealed at 1373 K in vacuum for only 5 days could be crystallized in the cubic NaZn13-type structure. The La0.8Ce0.2Fe11.4Si1.6 compound exhibited giant values of the isothermal entropy change ?SM around the Curie temperature TC (about 186 K). And the maximum value ΔS M max is about 78.29 J/(kg·K) under a field change of 0—3 T, which can be calculated by the magnetization iso- therms around TC. Such a large MCE is attributed to the sharp change of magnetization and susceptibility around TC and the first-order magnetic transition of field-induced IEMT above TC. | XU Chao LI Guodong LI Xiaowei WANG Ligang | 2006 | Chinese Science Bulletin2006，51，17： | 11 |
| 11 | Switched adaptive active disturbance rejection control of variable structure near space vehicles based on adaptive dynamic programming显示文摘Based on the switched nonlinear system, a switched adaptive Active Disturbance Rejection Control(ADRC) law is proposed for the Variable Structure Near Space Vehicle(VSNSV) with unknown uncertainties and external disturbances. The reduced-order Extended State Observers(ESOs) are constructed for the attitude angle system and the angular rate system to estimate the total disturbance in real time. With the extended state introduced to counteract the effects of uncertainties and disturbances, a systematic procedure is presented for the synthesis of the switched adaptive ADRC strategy. Rigorous proof shows that the estimation errors of the reduced-order ESOs would converge to a small neighborhood of zero in finite time, and that the output of the closedloop system can track a given signal stably for a class of switching signals with average dwell time via the proposed approach. The variable gain control strategy based on Adaptive Dynamic Programming(ADP) with the actor-critic structure is also designed to improve the dynamic performance of the system. Simulation results verify the effectiveness and advantage of the proposed control scheme. | Chaoyang DONG Chen LIU Qing WANG Ligang GONG | 2019 | Chinese Journal of Aeronautics2019，32，7： | 10 |
| 12 | Effect of the column height on the performance of liquid-solid fluidized bed for the separation of coarse slime显示文摘A liquid-solid fluidized bed separator,used for the separation of coarse slime,was developed.Test particles sized in the range from 0.25 to 0.5,0.5 to 1.0,and 0.25 to 1.0 mm were separated in the liquid-solid fluidized bed.Beds with column heights of 1200,1500,and 1800 mm were tried.The clean coal and the tailings were subsequently analyzed by float-sink testing.The results showed that the ash and yield of clean coal both decreased with increasing column height,for all three size fractions,and that the ash of the clean coal obtained from tests on the broader size fraction was less than that from the narrower sized fractions.The separation density decreased with increasing column height.The lowest E value was seen for a column height of 1500 mm,for which conditions the separation density was 1.45 g/cm 3.The E value was 0.084 for the 0.25-0.5 mm fraction but the corresponding separation density was 1.48 g/cm 3,and the E value 0.089,for the broader 0.25-1.0 mm fraction. | Sha Jie Xie Guangyuan Wang Hong Liu Junzhang Tang Ligang | 2012 | International Journal of Mining Science and Technology2012，22，4： | 10 |
| 13 | Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population显示文摘Background: Runs of homozygosity(ROHs) are homozygous segments of the genome where the two haplotypes inherited from the parents are identical. The current availability of genotypes for a very large number of single nucleotide polymorphisms(SNPs) is leading to more accurate characterization of ROHs in the whole genome. Here,we investigated the occurrence and distribution of ROHs in 3,692 Large White pigs and compared estimates of inbreeding coefficients calculated based on ROHs(FROH), homozygosity(FHOM), genomic relationship matrix(FGRM)and pedigree(FPED). Furthermore, we identified genomic regions with high ROH frequencies and annotated their candidate genes.Results: In total, 176,182 ROHs were identified from 3,569 animals, and all individuals displayed at least one ROH longer than 1 Mb. The ROHs identified were unevenly distributed on the autosomes. The highest and lowest coverages of Sus scrofa chromosomes(SSC) by ROH were on SSC14 and SSC13, respectively. The highest pairwise correlation among the different inbreeding coefficient estimates was 0.95 between FROH_totaland FHOM, while the lowest was-0.083 between FGRMand FPED. The correlations between FPEDand FROHusing four classes of ROH lengths ranged from 0.18 to 0.37 and increased with increasing ROH length, except for ROH > 10 Mb. Twelve ROH islands were located on four chromosomes(SSC1, 4, 6 and 14). These ROH islands harboured genes associated with reproduction, muscular development, fat deposition and adaptation, such as SIRT1, MYPN, SETDB1 and PSMD4.Conclusion: FROHcan be used to accurately assess individual inbreeding levels compared to other inbreeding coefficient estimators. In the absence of pedigree records, FROHcan provide an alternative to inbreeding estimates.Our findings can be used not only to effectively increase the response to selection by appropriately managing the rate of inbreeding and minimizing the negative effects of inbreeding depression but also to help detect genomic regions with an effect on traits under selection. | Liangyu Shi Ligang Wang Jiaxin Liu Tianyu Deng Hua Yan Longchao Zhang Xin Liu Hongmei Gao Xinhua Hou Lixian Wang Fuping Zhao | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 10 |
| 14 | The COVID-19 Pandemic and Its Impact on the Global Economy: What Does It Take to Turn Crisis into Opportunity?显示文摘The COVID-19 pandemic broke out at a time when there were heightened uncertainties in the global economy.Understanding these uncertainties provides an important background for analyzing the impact of the pandemic on the global economy,assessing the effectiveness of policy measures in combating the pandemic and reviving the global economy,and predicting the trajectory of the economic recovery in the post-pandemic era.We analyze how COVID-19 would likely deepen an existing malaises in the global economy,and what could be done to address these problems while managing the economic recovery.We argue that three fundamental factors that could lead to a solid recovery in the post pandemic era are structural reform,new technology and re-integration.They could be managed by instituting a new'global social contract.'Supported by strong public policies at all levels,especially at national level,these three factors could bring about the salvation of the global economy as it recovers or re-emerges from the pandemic crisis. | Ligang Song Yixiao Zhou | 2020 | China & World Economy2020，28，4： | 9 |
| 15 | Association of renal function with cardiac reverse remodeling and long-term outcome in heart failure patients following cardiac resynchronization therapy显示文摘 | Cai Chi Hua Wei Ding Ligang Wang Jing Chen Keping Yang Xinwei Liu Zhimin Zhang Shu | 2014 | Chinese Medical Journal2014，，23： | 8 |
| 16 | Precipitation trends and variability from 1950 to 2000 in arid lands of Central Asia显示文摘Climate warming will cause differences in precipitation distribution and changes in hydrological cycle both at regional and global scales. Arid lands of Central Asia(ALCA), one of the largest arid regions at the middle latitudes in the world, is likely to be strongly influenced by climate warming. Understanding the precipitation variations in the past is an important prerequisite for predicting future precipitation trends and thus managing regional water resources in such an arid region. In this study, we used run theory, displacement, extreme deviation theory, precipitation concentration index(PCI), Mann-Kendall rank correlation and climatic trend coefficient methods to analyze the precipitation in wet and dry years, changes in precipitation over multiple-time scales, variability of precipitation and its rate of change based on the monthly precipitation data during 1950–2000 from 344 meteorological stations in the ALCA. The occurrence probability of a single year with abundant precipitation was higher than that of a single year with less precipitation. The average duration of extreme drought in the entire area was 5 years, with an average annual water deficit of 34.6 mm(accounting for 11.2% of the average annual precipitation over the duration). The occurrence probability of a single wet year was slightly higher than that of a single dry year. The occurrence probability of more than 5 consecutive wet years was 5.8%, while the occurrence probability of more than 5 consecutive dry years was 6.2%. In the center of the study area, the distribution of precipitation was stable at an intra-annual timescale, with small changes at an inter-annual timescale. In the western part of the study area, the monthly variation of precipitation was high at an inter-annual timescale. There were clear seasonal changes in precipitation(PCI=12–36) in the ALCA. Precipitation in spring and winter accounted for 37.7% and 24.4% of the annual precipitation, respectively. There was a significant inter-annual change in precipitation in the arid Northwest China(PCI=24–34). Annual precipitation increased significantly(P=0.05) in 17.4% of all the meteorological stations over the study period. The probability of an increase in annual precipitation was 75.6%, with this increase being significant(P=0.05) at 34.0% of all the meteorological stations. The average increasing rate in annual precipitation was 3.9 mm/10a(P=0.01) in the ALCA. There were significant increasing trends(P=0.01) in precipitation in Kazakhstan, Kyrgyzstan and Tajikistan, with rates of 2.6, 3.1 and 3.7 mm/10 a, respectively. | XU Ligang ZHOU Hongfei DU Li YAO Haijiao WANG Huaibo | 2015 | Journal of Arid Land2015，7，4： | 8 |
| 17 | Pass-through Effects of Global Commodity Prices on China's Inflation:An Empirical Investigation显示文摘现在的纸使用一条二拍子的圆舞途径在中国在国内消费者价格通货膨胀上在国际商品价格和 RMB 汇率估计变化的传递效果。我们首先在制片人价格上估计国际商品价格的传递效果然后在消费者价格通货膨胀上估计制片人价格通货膨胀的传递效果。我们发现国际商品价格的 10 百分比增加将导致中国以后到 1.2% 3 个月增加的制片人价格,它将接着在一样的时期上增加中国的国内通货膨胀 0.24% 。然而,对对美元的 RMB 汇率的 10 百分比欣赏将帮助在下列 3 个月在 4.4% 在制片人价格减少增加,它将接着导致一个 0.89 百分比在一样的时期上在消费者价格通货膨胀衰退。我们的调查结果建议对在升起的全球商品价格和弱美元的环境的 RMB 的欣赏能是一台有效仪器帮助在中国包含通货膨胀。 | Ligang Liu Andrew Tsang | 2008 | China & World Economy2008，16，6： | 8 |
| 18 | Effects of rare earth elements La and Yb on the morphological and functional development of zebrafish embryos显示文摘In recent years,with the wide applications and mineral exploitation of rare earth elements,their potential environmental and health effects have caused increasing public concern.Effect of rare earth elements La and Yb on the morphological and functional development of zebrafish embryos were studied.The embryos were exposed to La3+ or Yb3+ at 0,0.01,0.1,0.3,0.5 and 1.0 mmol/L,respectively.Early life stage parameters such as egg and embryo mortality,gastrula development,tail detachment,eyes,somite formation,circulatory system,pigmentation,malformations,hatching rate,length of larvae and mortality were investigated.The results showed La3+ and Yb3+ delayed zebrafish embryo and larval development,decreased survival and hatching rates,and caused tail malformation in a concentration-dependent way.Moreover,heavy rare-earth ytterbium led to more severe acute toxicity of zebrafish embryo than light rare-earth lanthanum. | Jun'an Cui Zhiyong Zhang Wei Bai Ligang Zhang Xiao He Yuhui Ma Yan Liu Zhifang Chai | 2012 | Journal of Environmental Sciences2012，24，2： | 8 |
| 19 | An overview of energy and metabolic regulation显示文摘The physiology and behaviors related to energy balance are monitored by the nervous and humoral systems. Because of the difficulty in treating diabetes and obesity, elucidating the energy balance mechanism and identifying critical targets for treatment are important research goals. Therefore, the purpose of this article is to describe energy regulation by the central nervous system(CNS) and peripheral humoral pathway. Homeostasis and rewarding are the basis of CNS regulation. Anorexigenic or orexigenic effects reflect the activities of the POMC/CART or NPY/AgRP neurons within the hypothalamus. Neurotransmitters have roles in food intake, and responsive brain nuclei have different functions related to food intake, glucose monitoring, reward processing. Peripheral gut-or adipose-derived hormones are the major source of peripheral humoral regulation systems. Nutrients or metabolites and gut microbiota affect metabolism via a discrete pathway. We also review the role of peripheral organs, the liver,adipose tissue, and skeletal muscle in peripheral regulation. We discuss these topics and how the body regulates metabolism. | Song Wen Chaoxun Wang Min Gong Ligang Zhou | 2019 | Science China(Life Sciences)2019，62，6： | 8 |
| 20 | A study of miRNAs targets prediction and experimental validation显示文摘microRNAs(miRNAs)are 20–24 nucleotide(nt)RNAs that regulate eukaryotic gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs(mRNAs).To identify miRNA target genes will help a lot by understanding their biological functions.Sophisticated computational approaches for miRNA target prediction,and effective biological techniques for validating these targets now play a central role in elucidating their functions.Owing to the imperfect complementarity of animal miRNAs with their targets,it is difficult to judge the accuracy of the prediction.Complexity of regulation by miRNA-mediated targets at protein and mRNAs levels has made it more challenging to identify the targets.To date,only a few miRNAs targets are confirmed.In this article,we review the methods of miRNA target prediction and the experimental validation for their corresponding mRNA targets in animals. | Yong Huang Quan Zou Haitai Song Fei Song Ligang Wang Guozheng Zhang Xingjia Shen | 2010 | Protein & Cell2010，1，11： | 7 |
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