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| 1 | Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG显示文摘Two recently engineered SpCas9 variants, namely xCas9 and Cas9-NG, show promising potential in improving targeting specificity and broadening the targeting range. In this study, we evaluated these Cas9 variants in the model and crop plant, rice. We first tested xCas9-3.7, the most effective xCas9 variant in mammalian cells, for targeted mutagenesis at 16 possible NGN PAM (protospacer adjacent motif) combinations in duplicates. xCas9 exhibited nearly equivalent editing efficiency to wild-type Cas9 (Cas9-WT) at most canonical NGG PAM sites tested, whereas it showed limited activity at non-canonical NGH (H = A, C, T) PAM sites. High editing efficiency of xCas9 at NGG PAMs was further demonstrated with C to T base editing by both rAPOBECI and PmCDAI cytidine deaminases. With mismatched sgRNAs, we found that xCas9 had improved targeting specificity over the Cas9-WT. Furthermore, we tested two Cas9-NG variants, Cas9-NGv1 and Cas9-NG, for targeting NGN PAMs. Both Cas9-NG variants showed higher editing efficiency at most non-canonical NG PAM sites tested, and enabled much more efficient editing than xCas9 at AT-rich PAM sites such as GAT, GAA, and CAA. Nevertheless, we found that Cas9-NG variants showed significant reduced activity at the canonical NGG PAM sites. In stable transgenic rice lines, we demonstrated that Cas9-NG had much higher editing efficiency than Cas9-NGv1 and xCas9 at NG PAM sites. To expand the base-editing scope, we developed an efficient C to T base-editing system by making fusion of Cas9-NG nickase (D10A version), PmCDAI, and UGI. Taken together, our work benchmarked xCas9 as a high-fidelity nuclease for targeting canonical NGG PAMs and Cas9-NG as a preferred variant for targeting relaxed PAMs for plant genome editing. | Zhaohui Zhong Simon Sretenovic Qiurong Ren Lijia Yang Yu Bao Caiyan Qi Mingzhu Yuan Yao He Shishi Liu Xiaopei Liu Jiaheng Wang Lan Huang Yan Wang Dibin Baby David Wang Tao Zhang Yiping Qi Yong Zhang | 2019 | Molecular Plant2019，12，7： | 13 |
| 2 | Effect of Mo on High-Temperature Fatigue Behavior of 15CrNbTi Ferritic Stainless Steel显示文摘In order to understand the effect of Mo element on the high-temperature fatigue behavior of 15 CrNbTi ferritic stainless steel, the stress-controlled fatigue tests have been performed for both 15 CrNbTi and 15Cr0.5MoNbTi ferritic stainless steels at 800 °C in laboratory air. The fatigue test results indicate that the fatigue resistance of 15Cr0.5MoNbTi steel is manifestly higher than that of 15 CrNbTi steel at the maximum stress below 57 MPa; the 15Cr0.5MoNbTi steel possesses a fatigue limit of 35 MPa, which is higher than that of 15 CrNbTi steel. The TEM observations reveal that the Mo element can suppress the formation of coarsened Fe3Nb3 C precipitates and result in the fatigue resistance enhancement.The dislocation networks formed during the cyclic load favor to improve the fatigue resistance of 15Cr0.5MoNbTi steel at800 °C. | Tianlong Liu Lijia Chen Hongyun Bi Xin Che | 2014 | Acta Metallurgica Sinica(English Letters)2014，27，3： | 10 |
| 3 | Plant Genome Editing Using FnCpfl and LbCpf Nucleases at Redefined and Altered PAM-Sites显示文摘 | ZhaohuiZhong Yingxiao Zhang Qi You XU Tang Qiurong Ren Shishi Liu Lijia Yang Yan Wang Xiaopei Liu Binglin Liu Tao Zhang Xuelian Zheng Ysa Le Yong Zhang Yiping Qi | 2018 | Molecular Plant2018，11，7： | 10 |
| 4 | Targeted Mutagenesis of NAC Transcription Factor Gene,OsN AC041,Leading to Salt Sensitivity in Rice显示文摘Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt stress with various metabolism pathways. A number of plant transcription factor family genes have been found to be involved in the salt stress response, and NAM, ATAF and CUC(NAC) transcription factors are thought to act as active regulators during abiotic stress, especially salt stress. In this study, we detected a rice NAC transcription factor coding gene, OsNAC041, and confirmed that it influenced the germination of seeds under salt stress and salt tolerance of plants. OsNAC041 was primarily expressed in the leaves and located in the nucleus. Furthermore, the CRISPR/Cas9 method was used to obtain a targeted osnac041 mutant, of which the plant height was higher than that of the wild-type, showing increased salt sensitivity. Moreover, RNA-seq analysis revealed a number of differentially expressed genes(DEGs) involved in several important signaling pathways in the osnac041 mutant. Subsequently, Kyoto Encyclopedia of Genes and Genomes annotation also revealed differential expression of DEGs associated with mitogen-activated protein kinase signaling, peroxisome, eukaryotictype ABC transporters, photosynthesis and plant hormones, which are involved in stress-related signaling pathways. Overall, our study suggested that OsNAC041 was involved in the salt stress response in rice. These findings not only provide empirical evidence of OsNAC041 function, but also provide new insight into its potential application in rice resistance breeding. | WANG Bo ZHONG Zhaohui ZHANG Huanhuan WANG Xia LIU Binglin YANG Lijia HAN Xiangyan YU Deshui ZHENG Xuelian WANG Chunguo SONG Wenqin CHEN Chengbin ZHANG Yong | 2019 | Rice science2019，26，2： | 8 |
| 5 | Downregulation of caveolin-1 contributes to the synaptic plasticity deficit in the hippocampus of aged rats显示文摘Caveolin-1 is involved in the regulation of synaptic plasticity,but the relationship between its expression and cognitive function during aging remains controversial.To explore the relationship between synaptic plasticity in the aging process and changes in learning and memory,we examined caveolin-1 expression in the hippocampus,cortex and cerebellum of rats at different ages.We also examined the relationship between the expression of caveolin-1 and synaptophysin,a marker of synaptic plasticity.Hippocampal caveolin-1 and synaptophysin expression in aged(22–24 month old)rats was significantly lower than that in young(1 month old)and adult(4 months old)rats.Expression levels of both proteins were significantly greater in the cortex of aged rats than in that of young or adult rats,and levels were similar between the three age groups in the cerebellum.Linear regression analysis revealed that hippocampal expression of synaptophysin was associated with memory and learning abilities.Moreover,synaptophysin expression correlated positively with caveolin-1 expression in the hippocampus,cortex and cerebellum.These results confirm that caveolin-1 has a regulatory effect on synaptic plasticity,and suggest that the downregulation of hippocampal caveolin-1 expression causes a decrease in synaptic plasticity during physiological aging. | Yang Liu Zhanhua Liang Jing Liu Wei Zou Xiaoyan Li Yachen Wang Lijia An | 2013 | Neural Regeneration Research2013，8，29： | 6 |
| 6 | Phylogeography,Transmission,and Viral Proteins of Nipah Virus显示文摘Nipah virus (NiV), a zoonotic paramyxovirus belonging to the genus Henipavirus, is classified as a Biosafety Level-4 pathogen based on its high pathogenicity in humans and the lack of available vaccines or therapeutics. Since its initial emergence in 1998 in Malaysia, this virus has become a great threat to domestic animals and humans. Sporadic outbreaks and person-to-person transmission over the past two decades have resulted in hundreds of human fatalities. Epidemiological surveys have shown that NiV is distributed in Asia, Africa, and the South Pacific Ocean, and is transmitted by its natural reservoir, Pteropid bats. Numerous efforts have been made to analyze viral protein function and structure to develop feasible strategies for drug design. Increasing surveillance and preventative measures for the viral infectious disease are urgently needed. | Bangyao Sun Lijia Jia Bilin Liang Quanjiao Chen Di Liu | 2018 | Virologica Sinica2018，33，5： | 6 |
| 7 | Acoustic emission generated during the gas sorption-desorption process in coal显示文摘An experimental system for monitoring the acoustic signals generated in coal during gas sorption and/or desorption was designed and the acoustic signals were observed under different gas pressures. The experimental results show that signals generated by the coal during gas adsorption are attenuated over time. Also, the signals are not continuous but are impulsive. The intensity of the signals generated during gas desorption is far smaller than that observed during adsorption. The signal seen during desorption remains essentially stable. Cycles of sorption and desorption cause acoustic emission signals that exhibit a memory effect, which depends upon the maximum gas pressure the sample was exposed to in earlier cycles. Lower pressures in subsequent cycles, compared to the maximum adsorption pressure in previous cycles, cause both the energy and impulse frequency to be lower than previously. On the contrary, a gas adsorption pressure that exceeds the maximum pressure seen by the sample during earlier cycles causes both the energy and impulse frequency to be high. | Ma Yankun Wang Enyuan Xiao Dong Li Zhonghui Liu Jie Gan Lijia | 2012 | International Journal of Mining Science and Technology2012，22，3： | 5 |
| 8 | Construction of chimeric inducible promoters by elicitors of rice fungal blast pathogen and their expression in transgenic rice显示文摘The promoter fragments of wheat GstA1 and potato Gst1 have been amplified by PCR, cloned and fused respectively to the minimal promoter sequence of rice actin gene (Act1)) and its 5’ untranslated leader sequence together with GUS. The constructs with 2 chimeric promoters (WGA and PGA) have been transferred into rice in order to analyze their inducibility patterns in transgenic rice plants. The results show that: WGA and PGA are both inducible by elicitors of Pyricularia oryzae in transgenic rice cells; the intron I of rice Act1 gene is important for the heterogenic expression of monocot and dicot promoter elements in rice; and the Act1 minimal promoter and its 5’untranslated leader sequence produced low level background expression in rice. | LU Huafei MING Xiaotian QU Lijia LIU Meihua LI Jing GU Hongya CHEN Zhangliang | 2000 | Chinese Science Bulletin2000，45，3： | 5 |
| 9 | Review of Coded Modulation Free Space Optical Communication System显示文摘This paper presents a constructive discussion and inclusive review on advancements in the coded modulation free-space optical(FSO) communication system and corresponding techniques. These techniques mainly include channel model,forward error correction(FEC) and modulation schemes. Firstly,a complete view of FSO system is presented which contains the description,the current research situation along with the advantages over the traditional radio frequency(RF) wireless communication and fiber optical communications. Then,the channel model,FEC,modulation schemes and complete system of coded modulation FSO are analyzed successively. At last,the review work of coded modulation FSO system is summarized and further improvements are prospected. | WANG Kaimin LIU Bo ZHANG Lijia ZHANG Qi TIAN Qinghua XIN Xiangjun | 2015 | China Communications2015，12，11： | 3 |
| 10 | Phenol Wastewater Degradation by Electrocatalytic Oxidation with RuO_2-IrO_2-SnO_2/Ti Anodes in the High Gravity Field显示文摘A novel high gravity multi-concentric cylinder electrodes-rotating bed(MCCE-RB) was developed for the electrocatalytic degradation of phenol wastewater in order to enhance the mass transfer with the self-made RuO_2-IrO_2-SnO_2/Ti anodes. The influences of electric current density, inlet liquid circulation flowrate, high gravity factor, sodium chloride concentration,and initial pH value on phenol degradation efficiency were investigated, with the optimal operating conditions determined. The results showed that under the optimal operating conditions covering a current density of 35 mA/cm^2, an inlet liquid circulation flowrate of 48 L/h, a high gravity factor of 20, a sodium chloride concentration of 8.5 g/L, an initial pH value of 6.5, a reaction time of 100 min, and an initial phenol concentration of 500 mg/L, the efficiency for removal of phenol reached 99.7%, which was improved by 10.4% as compared to that achieved in the normal gravity field. The tendency regarding the change in efficiency for removal of phenol, total organic carbon(TOC), and chemical oxygen demand(COD)over time was studied. The intermediates and degradation pathway of phenol were deduced by high performance liquid chromatography(HPLC). | Gao Jing Yan Junjuan Liu Youzhi Zhang Dongming Chen Lijia | 2018 | China Petroleum Processing & Petrochemical Technology2018，20，4： | 3 |
| 11 | Structural and expressional analysis of a cDNA that expresses predominantly in rice stamens显示文摘The development of stamen is an important stage during the sexual reproduction in higher plants. Studying the genes that predominantly or even specifically expressed in stamens will aid in understanding the molecular mechanism of stamen development. The structure of a rice CHS-like gene (D5) which predominantly expressed in anthers has been analysized, showing high structural similarity with two other anther-specific genes (A1 and BA42) from Brassica napus. Northern analysis also reveals that the three genes share a similar expression pattern and that the expression of D5 may relate to the early development of pollens. It is concluded that D5, A1 and BA42 represent a group of diverged CHS-like genes which are related to stamen development. | Yi Zhang Lijia Qu Meihua Liu Xiaoli Qin Hongya Gu Zhangliang Chen | 1998 | Chinese Science Bulletin1998，43，9： | 2 |
| 12 | Toward calibration-free Mach-Zehnder switches for next-generation silicon photonics显示文摘Silicon photonic Mach–Zehnder switches(MZSs)have been extensively investigated as a promising candidate for optical systems.However,conventional 2×2 MZSs are usually prone to the size variations of the arm waveguides due to imperfect fabrication,resulting in considerable random phase imbalance between the two arms,thereby imposing significant challenges for further developing next-generation N×N MZSs.Here we propose a novel design toward calibration-free 2×2 and N×N MZSs,employing optimally widened arm waveguides,enabled by novel compact tapered Euler S-bends with incorporated mode filters.With standard 180 nm CMOS foundry processes,more than thirty 2×2 MZSs and one 4×4 Benes MZS with the new design are fabricated and characterized.Compared with their conventional counterparts with 0.45-μm-wide arm waveguides,the present 2×2 MZSs exhibit significant reduction in the random phase imbalance.The measured extinction ratios of the present 2×2 and 4×4 MZSs operating in the all-cross state are 27-49 dB and∼20dB across the wavelength range of∼60nm,respectively,even without any calibrations.This work paves the way toward calibration-free large-scale N×N MZSs for next-generation silicon photonics. | Lijia Song Tangnan Chen Weixi Liu Hongxuan Liu Yingying Peng Zejie Yu Huan Li Yaocheng Shi Daoxin Dai | 2022 | Photonics Research2022，10，3： | 2 |
| 13 | Ecosystem service valuation of bays in East China Sea and its response to sea reclamation activities显示文摘Ecosystem service values(ESVs)of bays and their response to sea reclamation are of great practical importance for forming bay eco-compensation policy and extension of blue economic space.Based on land use information of bays collected during the period of 1990–2015,the spatiotemporal evolution of ESVs of 12 main bays in East China Sea and their response to sea reclamation activities over the past 25 years were quantitatively analyzed.The analysis results indicate that ESVs of bays in East China Sea showed a continuous downward trend and the whole ecosystem was continuously degraded,in which the degradation degree of ESV in the southern bays was higher than that in the northern bays.Spatial zoning of ESVs of bays in East China Sea was remarkable,showing a continuous downward trend from low-value to high-value zone.Spatial variation of ESVs of each bay was also significant,expanding from a city and from inland to the coast,which suggests that human activities,mainly reclamation,have become main agents for ESV evolution of bays in East China Sea.ESVs of bays have a significant response to sea reclamation,manifested as a significant negative correlation between ESV and reclamation intensity.The correlation in the southern bays was stronger than that in the northern bays,which was caused by different effects of sea reclamation modes on ESV evolution of muddy and bedrock bays.A negative effect of sea reclamation activities on bay ecosystem was hysteretic.Therefore,an attention should be paid to dynamic monitoring and early warning of development status in offshore areas,ecosystem-level reclamation control policy,and coastal wetland reserves planning.Moreover,the spatial coupling mechanism study between bay ecosystem service demand and its service supply capacity should be strengthened to realize systematic regulation of bay ecological security pattern. | LIU Ruiqing XU Hao LI Jialin PU Ruiliang SUN Chao CAO Luodan JIANG Yimei TIAN Peng WANG Lijia GONG Hongbo | 2020 | Journal of Geographical Sciences2020，30，7： | 2 |
| 14 | An anther-specific chalcone synthase-like gene D5 related to rice pollen development显示文摘It was shown in a previous analysis that D5 gene from rice (Oryza sativa L.) was an anther-specific gene encoding a chalcone synthase-related protein. In this study, D5 gene was found specifically expressed in tapetum cells as well as in the peripheral cells of the vascular bundle of rice anthers by RNA in situ hybridization. In order to study its function, D5 was transformed into rice in both sense and antisense directions driven by a rice Actin 1 promoter. It has been observed that the pollen grains from the antisense D5 transgenic rice plants are abnormal, indicating that D5 plays a critical role in rice pollen development. | Honghong Zheng Lijia Qu Meihua Liu Yi Zhang Yunping Shen Junming Wei Naisui Pan Hongya Gu Zhangliang Chen | 2000 | Chinese Science Bulletin2000，45，21： | 2 |
| 15 | Viability of high-density resistivity method for evaluating mountain slope stability in Erdaojiang District,Tonghua City,China显示文摘The authors employ the high-density resistivity method to image the subsurface structure of a mountain in Erdaojiang District,Tonghua City,Jilin Province,China,to evaluate the potential risk of slope failure on surrounding residential areas and infrastructure,and identify a shallow fault that extends across the center of the mountain and is perpendicular to the mountain slope and accurately locate the spatial position and depth of another fault on the southern side of the mountain.The results provide an important basis for evaluating mountain slope stability.This study also demonstrates that the high-density resistivity method is effective for detecting mountain faults. | XIN Zhonghua HAN Jiangtao LI Zhuoyang LIU Lijia | 2020 | Global Geology2020，23，4： | 2 |
| 16 | Application of high-density resistivity method to evaluate safety around Minyan Tunnel,Helong City显示文摘By determining the distribution and extent of geological structures surrounding the Mingyan Tunnel,Xicheng Town,Helong City,Jilin Province,we can evaluate the stability of the rock mass and assess potential hazards during tunnel construction.We use the high-density resistivity method to analyze the subsurface structure of the study area.Conductive anomalies are likely to represent joint and fissure systems within strongly weathered host rocks,and the bedrock surrounding the tunnel is relatively stable and does not contain well-developed faults.High-density resistivity analysis can provide valuable information in the context of tunnel engineering and safety. | LI Zhuoyang HAN Jiangtao XIN Zhonghua LIU Lijia | 2020 | Global Geology2020，23，4： | 2 |
| 17 | False anomaly recognition with horizontal differential field lines method in high-density electrical technique and its application in Xinlei Quarry,Jiuquan显示文摘As an important geophysical tool,high density electrical technique infers the underground geological structures by processing and inverting the apparent resistivity data.Currently,the false anomalies have been frequently occurred in the graph of apparent resistivity pseudo-section or inverted geoelectrical section obtained from high-density electrical technique,and are difficult to remove.In this study,the authors explain the mechanism of the false anomalies and put forward the horizontal differential field method to identify the false anomalies.Based on the analysis of modeling results,this method is applied in the surveying data in Xinlei Quarry of Jiuquan,and the results confirm the effectiveness of the horizontal differential field method. | LIU Lijia ZHU Shi CAO Xiaoyue BEN Fang YIN Changchun | 2015 | Global Geology2015，18，2： | 2 |
| 18 | Corrigendum to “The Tianwen-1 Guidance, Navigation, and Control for Mars Entry, Descent, and Landing”显示文摘In the article titled“The Tianwen-1 Guidance,Navigation,and Control for Mars Entry,Descent,and Landing”[1],there was an error in Figure 9(b).On the y-axis,the data read q1,q2,and q1.It should have read q1,q2,and q3.This is updated as shown in Figure 1 below. | Xiangyu Huang Maodeng Li Xiaolei Wang Jinchang Hu Yu Zhao Minwen Guo Chao Xu Wangwang Liu Yunpeng Wang Ce Hao Lijia Xu | 2021 | Space(Science & Technology)2021，，1： | 2 |
| 19 | Enhanced zinc storage performance of mixed valent manganese oxide for flexible coaxial fiber zinc-ion battery by limited reduction control显示文摘While manganese-based cathodes have been intensively studied for zinc-ion batteries(ZIBs),the limited rate capability and cycle life have always been a difficult problem to be solved.Here,we report a mixed valent manganese oxide(MnOx)cathode with superior electrochemical performance,which exhibits a high specific capacity of 450 mA h/g at 0.2 C and a satisfactory specific capacity of 158.3 mA h/g at a high rate of 5 C.The mixed cathode system reduces the charge transfer resistance,and show good surface stability and adsorption properties,so it is beneficial for the storage of Zn^(2+).Meanwhile,coaxial fiber ZIBs(CFZIBs)with splendid flexibility are assembled utilizing the elaborately prepared cathode material.The CFZIBs achieve a reversible capacity of 255.8 m A h/g and the capacity retention rate is as high as 80%after 1000 bending deformations.This study provides new opportunities for designing ZIBs with high performance and high flexibility. | Xiaobei Zang Li Lingtong Jiaxin Meng Lijia Liu Yuanyuan Pan Qingguo Shao Ning Cao | 2021 | Journal of Materials Science & Technology2021，，15： | 2 |
| 20 | E3 ligase FBXW7 aggravates TMPD-induced systemic lupus erythematosus by promoting cell apoptosis显示文摘Systemic lupus erythematosus(SLE)is a systemic autoimmune disease,and the pathogenesis of SLE has not been fully elucidated.The E3 ubiquitin ligase FBXW7 has been well characterized in cancer as a tumor suppressor that can promote the ubiquitination and subsequent degradation of various oncoproteins;however,the potential role of FBXW7 in autoimmune diseases is unclear.In the present study,we identified that FBXW7 is a crucial exacerbating factor for SLE development and progression in a mouse model induced by 2,6,10,14-tetramethylpentadecane(TMPD).Myeloid cell-specific FBXW7-deficient(Lysm+FBXW7f/f)C57BL/6 mice showed decreased immune complex accumulation,glomerulonephritis,glomerular mesangial cell proliferation,and basemembrane thickness in the kidney.Lysm+FBXW7f/f mice produced fewer anti-Sm/RNP and anti-ANA autoantibodies and showed a decreased MHC II expression in B cells.In Lysm+FBXW7f/f mice,we observed that cell apoptosis was reduced and that fewer CD11b+Ly6Chi inflammatory monocytes were recruited to the peritoneal cavity.Consistently,diffuse pulmonary hemorrhage(DPH)was also decreased in Lysm+FBXW7f/f mice.Mechanistically,we clarified that FBXW7 promoted TMPD-induced cell apoptosis by catalyzing MCL1 degradation through K48-linked ubiquitination.Our work revealed that FBXW7 expression in myeloid cells played a crucial role in TMPD-induced SLE progression in mice,which may provide novel ideas and theoretical support for understanding the pathogenesis of SLE. | Zhenlu Chong Chunjing Bao Jia He Tianxiao Chen Lijia Zhong Gaopeng Li Huanle Li Lutong Fang Yinjing Song Guoxiang Fu Xuyan Yang Lihua Lai Yang Liu Qingqing Wang | 2018 | Cellular & Molecular Immunology2018，15，12： | 2 |
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