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| 1 | Targeted Mutagenesis of NAC Transcription Factor Gene,OsN AC041,Leading to Salt Sensitivity in Rice显示文摘Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt stress with various metabolism pathways. A number of plant transcription factor family genes have been found to be involved in the salt stress response, and NAM, ATAF and CUC(NAC) transcription factors are thought to act as active regulators during abiotic stress, especially salt stress. In this study, we detected a rice NAC transcription factor coding gene, OsNAC041, and confirmed that it influenced the germination of seeds under salt stress and salt tolerance of plants. OsNAC041 was primarily expressed in the leaves and located in the nucleus. Furthermore, the CRISPR/Cas9 method was used to obtain a targeted osnac041 mutant, of which the plant height was higher than that of the wild-type, showing increased salt sensitivity. Moreover, RNA-seq analysis revealed a number of differentially expressed genes(DEGs) involved in several important signaling pathways in the osnac041 mutant. Subsequently, Kyoto Encyclopedia of Genes and Genomes annotation also revealed differential expression of DEGs associated with mitogen-activated protein kinase signaling, peroxisome, eukaryotictype ABC transporters, photosynthesis and plant hormones, which are involved in stress-related signaling pathways. Overall, our study suggested that OsNAC041 was involved in the salt stress response in rice. These findings not only provide empirical evidence of OsNAC041 function, but also provide new insight into its potential application in rice resistance breeding. | WANG Bo ZHONG Zhaohui ZHANG Huanhuan WANG Xia LIU Binglin YANG Lijia HAN Xiangyan YU Deshui ZHENG Xuelian WANG Chunguo SONG Wenqin CHEN Chengbin ZHANG Yong | 2019 | Rice science2019，26，2： | 8 |
| 2 | Construction of a Metagenomic DNA Library of Sponge Symbionts and Screening of Antibacterial Metabolites显示文摘为了学习简历,活跃代谢物由导出海绵的未耕作的共生者生产了,海绵 Gelliodes gracilis 的共生者的一个 metagenomic DNA 图书馆被构造。DNA 的平均尺寸在图书馆插入是 20kb。这个图书馆用纸圆盘 assaying 为抗菌素活动被屏蔽。二克隆对小球菌 tetragenus 显示了抗菌剂活动。这二克隆的代谢物通过 HPLC 被分析。结果证明他们的代谢物与主人 E 的那些相当不同。包含向量 pHZ132 的关口 i DH5 α和主人。这研究可以介绍一条新途径给探索简历海绵共生者的活跃代谢物。 | CHEN Juan ZHU Tianjiao LI Dehai CUI Chengbin FANG Yuchun LIU Hongbing LIU Peipei GU Qianqun ZHU Weiming | 2006 | Journal of Ocean University of China2006，5，2： | 3 |
| 3 | Biodegradable nanocarriers for small interfering ribonucleic acid (siRNA) co-delivery strategy increase the chemosensitivity of pancreatic cancer cells to gemcitabine显示文摘Ribonucleic acid (RNA) interference (RNAi) therapies are promising cancertreatment modalities that can specifically target abnormal proto-oncogenes, thusimproving the therapeutic effect. For the treatment of pancreatic cancer, targetingone mutant proto-oncogene by RNAi usually does not yield the desired therapeuticefficiency. Both K-ras gene mutations and Notch1 overexpression are commonsymptoms in pancreatic cancer patients, and play a crucial role in pancreaticcancer cell drug resistance. In this study, biodegradable charged polyester-basedvectors (BCPVs) were synthesized for the co-delivery of K-ras and Notch1 smallinterfering ribonucleic acid (siRNA) into MiaPaCa-2 cells (pancreatic cancercell line) to overcome drug resistance to gemcitabine (GEM), a first-line chem-otherapeutic drug used in the clinic. BCPVs could effectively absorb negative siRNAto form a capsule-like structure, prevent siRNA from nuclease digestion in theserum, and promote effective siRNA cell internalization and endosomal escape.Through K-ras and Notch1 gene silencing in MiaPaCa-2 cells, BCPV-siRNAKsiRNAN^tcm nanocomplexes effectively reversed the epithelia-mesenchymaltransition (EMT) in MiaPaCa-2 cells, thereby greatly enhancing the sensitivity ofMiaPaCa-2 cells to GEM. MiaPaCa-2 cell proliferation, migration, and invasionwere effectively inhibited, and cell apoptosis was also significantly enhanced bythe synergistic antitumor effect of BCPV-siRNAK siRNAN^t~l nanocomplexesand GEM. These results suggest that this combination RNAi therapy can be usedto improve cancer cell sensitivity to chemotherapeutic drugs. Specifically, thisnewly develooed strate^v has a great potential for treating pancreatic cancer. | Chengbin Yang Kok Ken Chan Wen-Jen Lin Alana Mauluidy Soehartono Guimiao Lin Huiting Toh Ho Sup Yoon Chih-Kuang Chen Ken-Tye Yong | 2017 | Nano Research2017，10，9： | 3 |
| 4 | A LAGRANGIAN RELAXATION APPROACH FOR SUPPLY CHAIN PLANNING WITH ORDER/SETUP COSTS AND CAPACITY CONSTRAINTS显示文摘A heuristic approach is developed for supply chain planning modeled as multi-item multi-levelcapacitated lot sizing problems. The heuristic combines Lagrangian relaxation (LR) with local search.Different from existing LR approaches that relax capacity constraints and/or inventory balanceconstraints, our approach only relaxes the technical constraints that each 0-1 setup variable must takevalue 1 if its corresponding continuous variable is positive. The relaxed problem is approximatelysolved by using the simplex algorithm for linear programming, while Lagrange multipliers are updatedby using a surrogate subgradient method that ensures the convergence of the dual problem in case ofthe approximate resolution of the relaxed problem. At each iteration, a feasible solution of the originalproblem is constructed from the solution of the relaxed problem. The feasible solution is furtherimproved by a local search that changes the values of two setup variables at each time. By taking theadvantages of a special structure of the lot-sizing problem, the local search can be implemented byusing a modified simplex algorithm, which significantly reduces its computation time. Numericalexperiments show that our approach can find very good solutions for problems of realistic sizes in ashort computation time and is more effective than an existing commercial optimization code. | Haoxun CHEN Chengbin CHUIndustrial System Optimization Laboratory Technology University of Troyes, France | 2003 | Systems Science and Systems Engineering2003，12，1： | 2 |
| 5 | Assistant pullback technique for main span closure of Sutong Bridge显示文摘Sutong Bridge is a cable-stayed steel box girder bridge with a main span of 1 088 m.The erection of upper structure adopts geometry control method and requires no change to the unit's size and the structure's none-stress geometry.Before main span closure,the cantilever of girder reaches 540.8 m,the structure state is noticeably influenced by external circumstances,the main span closure face great difficulty.By abstracting the advantage of the pullback method abroad and the domestic temperature-cutting method,a new assistant pullback method have put forward and bring into practice actually.In this paper,the analysis key point of practice conditions,key parameter of practice,main measures of the method and the performance is introduced. | Chen Ming Luo Chengbin Wu Qihe Zhang Yongtao You Xinpeng | 2009 | Engineering Sciences2009，7，1： | 2 |
| 6 | Environmental Analysis with 2D Transition-Metal Dichalcogenide-Based Field-Effect Transistors显示文摘Field-effect transistors(FETs)present highly sensitive,rapid,and in situ detection capability in chemical and biological analysis.Recently,two-dimensional(2D)transition-metal dichalcogenides(TMDCs)attract significant attention as FET channel due to their unique structures and outstanding properties.With the booming of studies on TMDC FETs,we aim to give a timely review on TMDCbased FET sensors for environmental analysis in different media.First,theoretical basics on TMDC and FET sensor are introduced.Then,recent advances of TMDC FET sensor for pollutant detection in gaseous and aqueous media are,respectively,discussed.At last,future perspectives and challenges in practical application and commercialization are given for TMDC FET sensors.This article provides an overview on TMDC sensors for a wide variety of analytes with an emphasize on the increasing demand of advanced sensing technologies in environmental analysis. | Xiaoyan Chen Chengbin Liu Shun Mao | 2020 | Nano-Micro Letters2020，12，8： | 2 |
| 7 | Thermal response of brick wall filled with phase change materials (PCM) under fluctuating outdoor temperatures 显示文摘 | ZHANG Chengbin CHEN Yongping WU Iangyu | 2011 | Energy and Buildings2011，43，12： | 1 |
| 8 | Cyclic scheduling of a hoist with time window constraints显示文摘 | CHEN Haoxun CHU Chengbin PROTH J M | 1998 | IEEE Transactions on Robotics and Automation1998，14，1： | 1 |
| 9 | Puerarin Attenuates Ovalbumin-Induced Lung Inflammation and Hemostatic Unbalance in Rat Asthma Model显示文摘 | Feng Dong Chengbin Wang Jinyan Duan Weiyi Zhang Daijun Xiang Mianyang Li Tzeng-Ji Chen | 2014 | Evidence-Based Complementary and Alternative Medicine2014，，： | 1 |
| 10 | An improvement of Lagrangean relaxation approach for job shop scheduling: a dynamic programming method 显示文摘 | CHEN Haoxun CHU Chengbin Proth J M | 1998 | IEEE Trans on Robotics and Automation1998，14，5： | 1 |
| 11 | An improvement of the Lagrangean relaxation approach for job shop scheduling:a dynamic programming method显示文摘 | Chen Haoxun Chu Chengbin Proth J M | | 0，，05： | 1 |
| 12 | Direct electrodeposition of reduced graphene oxide on glassy carbon electrode and its electrochemical application显示文摘 | Liuyun Chen Yanhong Tang Ke Wang Chengbin Liu Shenglian Luo | 2010 | Electrochemistry Communications2010，，2： | 1 |
| 13 | Bubble breakup in a microfluidic T-junction显示文摘我们进行计算液体动力学模拟用 volume-of-fluid 方法在 microfluidic T 连接调查水泡决裂的行为代表接口。水泡形态学的进化和流动地里的速度和压力的分布被分析,并且在水泡形态学上的主要隧道和分支之间的宽度比率的效果被评估。结果显示那,隧道决裂,妨碍的决裂,联合决裂和非决裂在不同状况下面通过 T 连接在水泡流动期间被观察。整个水泡决裂进程经历扩展,压榨并且拧离开阶段,当非决裂进程经历扩展,推上演时。当在水泡内的速度具有为妨碍的决裂的寓言的分布时,我们发现在 squeeze 阶段,本地旋涡流动为隧道决裂在水泡的前面边形成。不管非决裂政体,有突然的压力落下,在 squeeze 发生在水泡的煤气液体的接口舞台,和在前面接口的压力落下在消沉区域比那远大。通过 T 连接的水泡决裂政体的转变随主要隧道的宽度比率的增加发生到分支,它顺序经历非决裂政体,隧道决裂政体和妨碍的决裂政体。流动政体图与幂定律关联被阴谋区分水泡 / 微滴决裂和非决裂政体,它也通过 T 连接描绘水泡和微滴决裂之间的差别。 | Xiangdong Liu Chengbin Zhang Wei Yu Zilong Deng Yongping Chen | 2016 | Science Bulletin2016，61，10： | 1 |
| 14 | Study on Flow and Heat Transfer Characteristics of Heat Pipewith Axial'Ω”Shaped Microgrooves显示文摘 | CHEN Yongping ZHANG Chengbin SHI Mingheng | | 0，，： | 1 |
| 15 | Conservation and characterization of unique porcine interstitial telomeric sequences显示文摘Telomeres are composed of TTAGGG repeats and located at the ends of chromosomes. Telomeres protect chromosomes from instability in mammals, including mice and humans. Repetitive TTAGGG sequences are also found at intrachromosomal sites, where they are named as interstitial telomeric sequences (ITSs). Aberrant ITSs are implicated in chromosomal instability and found in cancer cells. Interestingly, in pigs, vertebrate telomere sequences TTAGGG (vITSs) are also localized at the centromeric region of chromosome 6, in addition to the end of all chromosomes. Surprisingly, we found that botanic telomere sequences, TTTAGGG (bITSs), also localize with vITSs at the centromeric regions of pig chromosome 6 using telomere fluorescence in situ hybridization (FISH) and by comparisons between several species. Furthermore, the average lengths of vITSs are highly correlated with those of the terminal telomeres (TTS). Also, pig ITSs show a high incidence of telomere doublets, suggesting that pig ITSs might be unstable and dynamic. Together, our results show that pig cells maintain the conserved telomere sequences that are found at the ITSs from of plants and other vertebrates. Further understanding of the function and regulation of pig ITSs may provide new clues for evolution and chromosomal instability. | JI GuangZhen LIU Kai CHEN ChengBin RUAN WeiMin GLYTSOU Christina YANG Yang OKUKA Maja SONG WenQing GAGOS Sarantis LINing LIU Lin | 2012 | Science China(Life Sciences)2012，55，12： | 1 |
| 16 | A Novel Symbol Overlapping FFH-OCDMA system 显示文摘 | SHENG CHENGBIN WU CHEN YU J1NHUI | 2004 | Chinse Optics Letters2004，2，2： | 1 |
| 17 | A strategy to enhance the insecticidal potency of Vip3Aa by introducing additional cleavage sites to increase its proteolytic activation efficiency显示文摘Microbially derived,protein-based biopesticides have become a vital element in pest management strategies.Vip3 family proteins from Bacillus thuringiensis have distinct characteristics from known insecticidal Cry toxins and show efficient insecticidal activity against several detrimental lepidopteran pests.They are considered to be a promising toxic candidate for the management of various detrimental pests.In this study,we found that in addition to the preliminary digestion sites lysine,there are multiple cleavage activation sites in the linker region between domain I(DI)and DII of Vip3Aa.We further demonstrated that by adding more cleavage sites between DI and DII of Vip3Aa,its proteolysis efficiency by midgut proteases can be significantly increased,and correspondingly enhance its insecticidal activity against Spodoptera frugiperda and Helicoverpa armigera larvae.Our study promotes the understanding of the insecticidal mechanism of Vip3 proteins and illustrates an eas-ily implementable strategy to increase the insecticidal potency of Vip3Aa.This facilitates their potential future development and efficient application for sustainable agriculture. | Kun Jiang Zhe Chen Yiting Shi Yuanrong Zang Chengbin Shang Xi Huang Jiahe Zang Zhudong Bai Xuyao Jiao Jun Cai Xiang Gao | 2023 | Engineering Microbiology2023，3，4： | 1 |
| 18 | Simple and sensitive colorimetric detection of cysteine based on ssDNA-stabilized gold nanoparticles显示文摘 | Zhang Chen Shenglian Luo Chengbin Liu Qingyun Cai | 2009 | Analytical and Bioanalytical Chemistry2009，，2： | 1 |
| 19 | High-intensity focused ultrasound (HIFU): effective and safe therapy for hepatocellular carcinoma adjacent to major hepatic veins显示文摘 | Lian Zhang Hui Zhu Chengbin Jin Kun Zhou Kequan Li Haibing Su Wenzhi Chen Jin Bai Zhibiao Wang | 2009 | European Radiology2009，，2： | 1 |
| 20 | Simple and sensitive colorimetric detection of cysteine based on ssDNA-stabilized gold nanoparticles显示文摘 | Zhang Chen Shenglian Luo Chengbin Liu Qingyun Cai | 2009 | Analytical and Bioanalytical Chemistry2009，，2： | 1 |
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