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| 1 | Profiling kidney microRNAs from juvenile grass carp(Ctenopharyngodon idella) after 56 days of oral exposure to decabromodiphenyl ethane显示文摘Grass carp(Ctenopharyngodon idella) is one of the most important species in China.Decabromodiphenyl ethane(DBDPE) is a brominated flame retardant that has been used widely in industry, and has been observed to accumulate in the tissues of fish from South China. Evidence has shown that DBDPE is toxic to aquatic animals, but the molecular response has been unclear. MicroRNAs(miRNAs) are small noncoding and negative regulatory RNAs that are 20–24 nucleotides in length, which are involved in a wide range of biological processes. We took advantage of deep-sequencing techniques to accurately and comprehensively profile the kidney mi RNA expression of grass carp after 8 weeks of oral exposure to DBDPE. After mapping sequencing data to the genome and Expressed Sequence Tags(ESTs) of grass carp, we identified 493 miRNAs in the sequenced grass carp samples, which included 51 new miRNAs. The results indicated that 5 miRNAs were significantly down-regulated and 36 miRNAs were significantly up-regulated(FDR < 0.001,1.5-fold change) after DBDPE exposure. Real-time quantitative PCR(RT-q PCR) was performed on 4 miRNAs from the two samples, and the sequencing and RT-q PCR data were consistent. This study provides the first comprehensive identification of grass carp miRNAs, and the first expression analysis of grass carp miRNAs following DBDPE exposure.The results indicated that miRNAs have potential for use as biomarkers. | Lian Gan Yuanyan Xiong Fang Dong Yunjiang Yu Lijuan Zhang Shunmei E. Liliu Zhou Xiaoxia Li Guocheng Hu | 2016 | Journal of Environmental Sciences2016，28，6： | 4 |
| 2 | Two types of cinnamoyl-Co A reductase function divergently in accumulation of lignins,flavonoids and glucosinolates and enhance lodging resistance in Brassica napus显示文摘Brassica crops,which are of worldwide importance,provide various oil,vegetable and ornamental products,as well as feedstocks for animal husbandry and biofuel industry.Cinnamoyl-Co A reductase(CCR)is the entry point to the lignin pathway and a crucial locus in manipulation of associated traits,but CCRassociated metabolism and traits in Brassica crops have remained largely unstudied except in Arabidopsis thaliana.We report the identification of 16 CCR genes from Brassica napus and its parental species B.rapa and B.oleracea.The Bn CCR1 and Bn CCR2 subfamilies displayed divergent organ-specificity and participation in the yellow-seed trait.Their functions were dissected via overexpression of representative paralogs in B.napus.Bn CCR1 was expressed preferentially in G-and H-lignin biosynthesis and vascular development,while Bn CCR2 was expressed in S-lignin biosynthesis and interfascicular fiber development.Bn CCR1 showed stronger effects on lignification-related development,lodging resistance,phenylpropanoid flux control,and seed coat pigmentation,whereas Bn CCR2 showed a stronger effect on sinapate biosynthesis.Bn CCR1 upregulation delayed bolting and flowering time,while Bn CCR2 upregulation weakened the leaf vascular system in consequence of suppressed G-lignin accumulation.Bn CCR1 and Bn CCR2 were closely but almost oppositely linked with glucosinolate metabolism via inter-pathway crosstalk.We conclude that Bn CCR1 and Bn CCR2 subfamilies offer great but differing potential for manipulating traits associated with phenylpropanoids and glucosinolates.This study reveals the CCR1–CCR2 divergence in Brassicaceae and offers a resource for rapeseed breeding for lodging resistance,yellowseed traits,and glucosinolate traits. | Nengwen Yin Bo Li Xue Liu Ying Liang Jianping Lian Yufei Xue Cunmin Qu Kun Lu Lijuan Wei Rui Wang Jiana Li Yourong Chai | 2022 | The Crop Journal2022，10，3： | 1 |
| 3 | Clinical Characteristics of Endometrial Stromal Sarcoma From an Academic Medical Hospital in China显示文摘 | Ying Jin Lingya Pan Xueqing Wang Zhiqin Dai Huifang Huang Lina Guo Keng Shen Lijuan Lian | 2010 | International Journal of Gynecological Cancer2010，，9： | 1 |
| 4 | Selective Adsorption of Sulfur Compounds in Model Gasoline Containing Olefins by Modified Zeolites显示文摘石蜡的效果被测量修改沸石在房间温度和对模型燃料的吸附的 desulfurization 的石蜡的空气 pressure.The 影响经由改正床吸附技术在模型燃料( 300 ppm )与增加的石蜡集中移开的 thiophenes 的减少的数量确定被英尺红外技术调查了,它表明在沸石的质子是为 olefins.On 的吸附负责的地点 CeY 沸石。 | Wang Hongguo Song Lijuan Lian Peiyong Pan Mingxue Ju Xiufang Sun Zhaolin | 2010 | China Petroleum Processing & Petrochemical Technology2010，12，3： | 0 |
| 5 | Acquisition of a novel conjugative multidrug-resistant hypervirulent plasmid leads to hypervirulence in clinical carbapenem-resistant Klebsiella pneumoniae strains显示文摘The co-occurrence of plasmid-mediated multidrug resistance and hypervirulence in epidemic carbapenem-resistant Klebsiella pneumoniae has emerged as a global public health issue.In this study,an ST23 carbapenem-resistant hyper-virulent K.pneumoniae(CR-HvKP)strain VH1-2 was identified from cucumber in China and harbored a novel hybrid plasmid pVH1-2-VIR.The plasmid pVH1-2-VIR carrying both virulence and multidrug-resistance(MDR)genes was likely generated through the recombination of a virulence plasmid and an IncFIIK conjugative MDR plasmid in clinical ST2318622 isolated from a sputum sample.The plasmid pVH1-2-VIR exhibited the capacity for transfer to the clinical ST11 carbapenem-resistant K.pneumoniae(CRKP)strain via conjugation assay.Acquisition of pVH1-2-VIR plasmid directly converted a CRKP into CR-HvKP strain characterized by hypermucoviscosity,heightened virulence for Galleria mellonella larvae,and increased colonization ability in the mouse intestine.The emergence of such a hybrid plasmid may expedite the spread of CR-HvKP strains,posing a significant risk to human health. | Gong Li Ling Jia Lei Wan Lijuan Xia Ang Gao Runshi Yang Ruanyang Sun Minge Wang Juan Du Xinlei Lian Rongmin Zhang Liangxing Fang Xiaoping Liao Yahong Liu Bao-Tao Liu Jian Sun | 2023 | mLife2023，2，3： | 0 |
| 6 | Rice transcription factors OsLBD37/38/39 regulate nitrate uptake by repressing OsNRT2.1/2.2/2.3 under high-nitrogen conditions显示文摘Nitrate(NO_(3)^(-)) uptake involves a finely regulated and complex multilevel response system.Elucidating the molecular mechanism of nitrate uptake may lead to improving the growth and productivity of plants in the presence of dynamic variation in nitrate concentration.In this study,we identified three lateral organ boundaries domain(LBD)transcription factors,OsLBD37,OsLBD38,and OsLBD39,as regulators of nitrate uptake in response to nitrogen(N)availability.OsLBD37,OsLBD38,and OsLBD39 were induced by ammonium and glutamine in rice roots.Individual or collective knockout of OsLBD37,OsLBD38,and OsLBD39 led to increased concentrations of nitrate and increased expression of OsNRT2.1,OsNRT2.2,and OsNRT2.3respectively under high-N conditions,whereas overexpression of each of these three LBD genes produced opposite effects where N accumulation was reduced.Dual-luciferase reporter assay further confirmed that OsLBD37,OsLBD38,and OsLBD39 possessed transcription inhibitory activities in rice protoplast cells,downregulating the expression of OsNRT2.1/OsNRT2.2/OsNRT2.3.Yeast two-hybrid and bimolecular fluorescence complementation assays showed that OsLBD37 interacted with OsLBD37,OsLBD38,and OsLBD39in the nucleus.Together,these results show that OsLBD37,OsLBD38,and OsLBD39 collaborate to inhibit the expression of OsNRT2.1/OsNRT2.2/OsNRT2.3 transporters under N-sufficient conditions,thereby helping rice plants avoid excessive nitrate accumulation that may affect their growth. | Xinxin Zhu Dujun Wang Lijuan Xie Tao Zhou Jingyi Zhao Qian Zhang Meng Yang Wenjuan Wu Xingming Lian | 2022 | The Crop Journal2022，10，6： | 0 |
| 7 | RoTFL1c of Rosa multiflora has a dual-function in suppressing reproductive growth and promoting vegetative growth of Arabidopsis显示文摘Dear Editor, TFL1homologues play a key role in the flowering habit of roses.To analyze the function of the newly isolated TFL1 gene of Rosa multiflora(Chen et al.,2013),named RoTFLlc,we performed overexpression analyses in Arabidopsis thaliana.When compared with empty vector control and wild type Arabidopsis plants,RoTFLlc overexpressing transgenic plants exhibited strong phenotypes such as a clustered habit,an increased number of rosette leaves, late flowering or failure to flower. | Fang Wang Lijuan Lian Yunfeng Liu Yuman Zhang Rongxiang Fang Qinglin Liu | 2018 | Science China(Life Sciences)2018，61，12： | 0 |
| 8 | CeO_(2) doping boosted low-temperature NH3-SCR activity of FeTiOx catalyst:A microstructure analysis and reaction mechanistic study显示文摘FeTiOx has been recognized as an environmental-friendly and cost-effective catalyst for selective catalytic reduction(SCR)of NOx with NH3.Aimed at further improving the low-temperature DeNOx efficiency of FeTiOx catalyst,a simple strategy of CeO_(2) doping was proposed.The low-temperature(<250°C)NH3-SCR activity of FeTiOx catalyst could be dramatically enhanced by CeO2 doping,and the optimal composition of the catalyst was confirmed as FeCe_(0.2)TiOx,which performed a NOx conversion of 90%at ca.200°C.According to X-ray diffraction(XRD),Raman spectra and X-ray absorption fine structure spectroscopy(XAFS)analysis,FeCe_(0.2)TiOx showed low crystallinity,with Fe and Ce species well mixed with each other.Based on the fitting results of extended X-ray absorption fine structure(EXAFS),a unique Ce-O-Fe structure was formed in FeCe_(0.2)TiOx catalyst.The well improved specific surface area and the newly formed Ce-O-Fe structure dramatically contributed to the improvement of the redox property of FeCe_(0.2)TiOx catalyst,which was well confirmed by H2-temperature-programmed reduction(H2-TPR)and in situ XAFS experiments.Such enhanced redox capability could benefit the activation of NO and NH_(3) at low temperatures for NOx removal.The detailed reaction mechanism study further suggested that the facile oxidative dehydrogenation of NH_(3) to highly reactive-NH_(2) played a key role in enhancing the low-temperature NH_(3)-SCR performance of FeCe_(0.2)TiOx catalyst. | Wei Tan Shaohua Xie Wenpo Shan Zhihua Lian Lijuan Xie Annai Liu Fei Gao Lin Dong Hong He Fudong Liu | 2022 | Frontiers of Environmental Science & Engineering2022，16，5： | 0 |
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