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| 1 | Feature Extraction Method Based on Pseudo-Wigner-Ville Distribution for Rotational Machinery in Variable Operating Conditions显示文摘In the case of fault diagnosis for roller bearings, the conventional diagnosis approaches by using the time interval of energy impacts in time-frequency distribution or the pass-frequencies are based on the assumption that machinery operates under a constant rotational speed. However, when the rotational speed varies in the broader range, the pass-frequencies vary with the change of rotational speed and bearing faults cannot be identified by the interval of impacts. Researches related to automatic diagnosis for rotational machinery in variable operating conditions were quite few. A novel automatic feature extraction method is proposed based on a pseudo-Wigner-Ville distribution (PWVD) and an extraction of symptom parameter (SP). An extraction method for instantaneous feature spectrum is presented using the relative crossing information (RCI) and sequential inference approach, by which the feature spectrum from time-frequency distribution can be automatically, sequentially extracted. The SPs are considered in the frequency domain using the extracted feature spectrum to identify among the conditions of a machine. A method to obtain the synthetic symptom parameter is also proposed by the least squares mapping (LSM) technique for increasing the diagnosis sensitivity of SP. Practical examples of diagnosis for bearings are given in order to verify the effectiveness of the proposed method. The verification results show that the features of bearing faults, such as the outer-race, inner-race and roller element defects have been effectively extracted, and the proposed method can be used for condition diagnosis of a machine under the variable rotational speed. | WANG Huaqing LIKe SUN Hao CHEN Peng | 2011 | Chinese Journal of Mechanical Engineering2011，24，4： | 8 |
| 2 | Mycorrhizal symbiosis modulates the rhizosphere microbiota to promote rhizobia–legume symbiosis显示文摘Plants establish symbioses with mutualistic fungi,such as arbuscular mycorrhizal(AM)fungi,and bacteria,such as rhizobia,to exchange key nutrients and thrive.Plants and symbionts have coevolved and represent vital components of terrestrial ecosystems.Plants employ an ancestral AM signaling pathway to establish intracellular symbioses,including the legume–rhizobia symbiosis,in their roots.Nevertheless,the relationship between the AM and rhizobial symbioses in native soil is poorly understood.Here,we examined how these distinct symbioses affect root-associated bacterial communities in Medicago truncatula by performing quantitative microbiota profiling(QMP)of 16S rRNA genes.We found that M.truncatula mutants that cannot establish AM or rhizobia symbiosis have an altered microbial load(quantitative abundance)in the rhizosphere and roots,and in particular that AM symbiosis is required to assemble a normal quantitative root-associated microbiota in native soil.Moreover,quantitative microbial co-abundance network analyses revealed that AM symbiosis affects Rhizobiales hubs among plant microbiota and benefits the plant holobiont.Through QMP of rhizobial rpoB and AM fungal SSU rRNA genes,we revealed a new layer of interaction whereby AM symbiosis promotes rhizobia accumulation in the rhizosphere of M.truncatula.We further showed that AM symbiosis-conditioned microbial communities within the M.truncatula rhizosphere could promote nodulation in different legume plants in native soil.Given that the AM and rhizobial symbioses are critical for crop growth,our findings might inform strategies to improve agricultural management.Moreover,our work sheds light on the co-evolution of these intracellular symbioses during plant adaptation to native soil conditions. | Xiaolin Wang Huan Feng Yayu Wang Mingxing Wang Xingguang Xie Huizhong Chang Like Wang Jicheng Qu Kai Sun Wei He Chunyan Wang Chuanchao Dai Zhaohui Chu Changfu Tian Nan Yu Xuebin Zhang Huan Liu Ertao Wang | 2021 | Molecular Plant2021，14，3： | 6 |
| 3 | Transcriptome profiles reveal that gibberellin-related genes regulate weeping traits in crape myrtle显示文摘Plant architecture includes vital traits that influence and benefit crops,and economically important trees.Different plant architectures provide natural beauty.Weeping ornamental plants are aesthetically appealing to people.The regulatory mechanism controlling the weeping trait is poorly understood in crape myrtle.To investigate the weeping trait mechanism,transcriptional profiling of different organs in weeping and upright crape myrtle was performed based on phenotype.Phenotypic and histological analyses demonstrated that endodermal cells were absent,and that new shoot phenotypes could be rescued by the GA3 treatment of weeping plants.The transcriptional analysis and coexpression network analysis(WGCNA)of differentially expressed genes indicated that GA synthesis and signal transduction pathways play a role in weeping traits.When the expression level of a negative element of GA signaling,LfiGRAS1,was reduced by virus-induced gene silencing(VIGS),new branches grew in infected plants in a negatively geotropic manner.An integrated analysis implied that GA had a strong influence on weeping crape myrtle by interacting with other factors.This study helps to elucidate the mechanism governing the weeping trait and can improve the efficiency of breeding in Lagerstroemia. | Suzhen Li Tangchun Zheng Xiaokang Zhuo Zhuojiao Li Lulu Li Ping Li Like Qiu Huitang Pan Jia Wang Tangren Cheng Qixiang Zhang | 2020 | Horticulture Research2020，7，1： | 5 |
| 4 | Influence of the Addition of Cotton Stalk during Co-pyrolysis with Sewage Sludge on the Properties, Surface Characteristics, and Ecological Risks of Biochars显示文摘Sewage sludge produced by municipal sewage treatment plants can potentially be used as a biomass energy source because of its high organic content.Presently,the conversion and utilization of rapidly growing amounts of sewage sludge represent an urgent challenge in China.Thermal conversion of sewage sludge to biochar through pyrolysis is a promising solution to this problem.However,biochar produced by pyrolysis of sewage sludge alone has a poor pore structure as a result of its low C content and high ash content.Furthermore,it is enriched in heavy metals that may pose high ecological risks.In this study,we addressed these issues through co-pyrolysis of sewage sludge and cotton stalks(1:1,wt./wt.)at different pyrolysis temperatures ranging from 350℃ to 750℃.The properties and surface characteristics of the biochars were investigated.Meanwhile,the transformation behavior of heavy metals during the co-pyrolysis process was studied,and the potential ecological risks of heavy metals in biochars were assessed.The results showed that elevated pyrolysis temperatures reduced the biochar yield and C content of the biochars,whereas such temperatures increased the pH value and ash content of the biochars.The biochars prepared at different pyrolysis temperatures were all mesoporous materials.The elevated temperatures promoted the transformation of heavy metals from mobile fractions to stable ones,thus resulting in a significant decrease in the ecological risks.In summary,co-pyrolysis of sewage sludge with cotton stalks proved to be a feasible method for the conversion and utilization of sewage sludge. | WANG Zhipu WANG Jian XIE Like ZHU Henan SHU Xinqian | 2019 | Journal of Thermal Science2019，28，4： | 5 |
| 5 | Clinical and pathological features and surgical treatment of Budd-Chiari syndrome-associated hepatocellular carcinoma显示文摘 | WANG Ya-dong XUE Huan-zhou ZHANG Xiao XU Zong-quan JIANG Qing-feng SHEN Quan YU Miao LIKe JIA Meng | 2013 | Chinese Medical Journal2013，，19： | 4 |
| 6 | Cell viability and dopamine secretion of 6-hydroxydopamine-treated PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells显示文摘In the present study, PC12 cells induced by 6-hydroxydopamine as a model of Parkinson's Disease, were used to investigate the protective effects of bone marrow-derived mesenchymal stem cells bone marrow-derived mesenchymal stem cells against 6-hydroxydopamine-induced neurotoxicity and to verify whether the mechanism of action relates to abnormal α-synuclein accumulation in cells. Results showed that co-culture with bone marrow-derived mesenchymal stem cells enhanced PC12 cell viability and dopamine secretion in a cell dose-dependent manner. MitoLight staining was used to confirm that PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells demonstrate reduced levels of cell apoptosis. Immunocytochemistry and western blot analysis found the quantity of α-synuclein accumulation was significantly reduced in PC12 cell and bone marrow-derived mesenchymal stem cell co-cultures. These results indicate that bone marrow-derived mesenchymal stem cells can attenuate 6-hydroxydopamine-induced cytotoxicity by reducing abnormal α-synuclein accumulation in PC12 cells. | Yue Tang Yongchun Cui Fuliang Luo Xiaopeng Liu Xiaojuan Wang Aili Wu Junwei Zhao Zhong Tian Like Wu | 2012 | Neural Regeneration Research2012，7，14： | 3 |
| 7 | Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs显示文摘The miniature pig is an optimal animal model for studying nervous system disease because of its physiologic and pathologic features. However, the rete mirabile composed of arteries and veins at the skull base limits their application as a model of ischemic stroke by middle cerebral artery occlusion. The present study investigated the possibility of establishing an ischemic stroke model in the miniature pig by blocking the skull base retia with sodium alginate microspheres. Three Bama miniature pigs were used. Using the monitor of C-arm X-ray machine, sodium alginate microspheres (100-300 μm), a novel embolic material, were injected through the femoral artery, aortic arch, common carotid artery, ascending pharyngeal artery and the retia. Results were evaluated using carotid arteriography, MRI, behavior observation and histology. The unilateral rete mirabile was completely blocked, resulting in disturbance in blood supply to the basal ganglia, astasia of the right hind limb and salivation. MRI and hematoxylin-eosin staining showed an evident infarction focus in the basal ganglia. These findings indicate that sodium alginate microspheres are a suitable embolic material for blocking the skull base retia in miniature pigs to establish an ischemic stroke models. | Yongchun Cui Yi Tian Yue Tang Liujun Jia Aili Wu Peng Peng Jianzhong Yang Hong Du Xiaojuan Wang Like Wu | 2013 | Neural Regeneration Research2013，8，16： | 2 |
| 8 | Overexpression of a cotton annexin gene, GhAnn1 , enhances drought and salt stress tolerance in transgenic cotton显示文摘 | Feng Zhang Shufen Li Shuming Yang Like Wang Wangzhen Guo | 2015 | Plant Molecular Biology . 2015 (1-2)2015，，1： | 1 |
| 9 | Effects of treadmill exer- cise training on liver fat accumulation and estrogen receptor alpha ex- pression in intact and ovariectomized rats with or without estrogen 显示文摘 | Like Hao Yijing Wang Yushuang Duan | 2010 | Eur J Appl Physiol2010，109，5： | 1 |
| 10 | SJES14110700417215显示文摘 | Jiachen Wang Shengli Yang Yang Li Like Wei Haohao Liu | 2014 | International Journal of Rock Mechanics and Mining Sciences2014，，： | 1 |
| 11 | Preparation of nano BaTiO3-based ceramics for multilayer ceramic capacitor application by chemical coating method显示文摘 | Tian Zhibin Wang Xiaohui Shu Like | 2009 | J Am Ceram Soc2009，92，： | 1 |
| 12 | Numerical simulation and analysis of low hysteresis brush seals 显示文摘 | CHI Jiadong WANG Zhili LI Like | 2012 | Advanced Materials Research2012，，： | 1 |
| 13 | Apeli protects heart against ischemia/reperfusion injury in rat 显示文摘 | Zeng Xiangjun Zhang Like Wang Hongxia | 2009 | Peptides2009，30，6： | 1 |
| 14 | in-situ Al-Si hypereutectic alloy FGMs prepared by electromagnetic separation 显示文摘 | LIKe WANG Jun SHU Da | 2002 | J Mater Sci &Tech2002，18，5： | 1 |
| 15 | Dietary glutamine supplementation improves growth performance, meat quality, and colour stability of broilers under heat stress 显示文摘 | Dai Sifa Wang Like Wen Aiyou | 2009 | British Poultry Science2009，50，3： | 1 |
| 16 | Apelin protects heart against ischemia/reperfusion injury in rat 显示文摘 | Zeng Xiangjun Zhang Like Wang Hongxia | 2009 | Peptides2009，30，6： | 1 |
| 17 | Does precut technique improve selective bile duct cannulation or increase post-ERCP pancreatitis rate? A meta-analysis of randomized controlled trials显示文摘 | Biao Gong Lixiao Hao Like Bie Bo Sun Mei Wang | 2010 | Surgical Endoscopy2010，，11： | 1 |
| 18 | Theoretical and experimental investigation of aluminum melt cleaning using alternating electromagnetic field 显示文摘 | LIKe WANG Jun SHU Da | 2002 | Mater Lett2002，56，10： | 1 |
| 19 | The allosteric modulation effects of doxycycline, minocycline, and their derivatives on the neuropeptide receptor PAC1-R显示文摘Class B G-protein coupled receptors (GPCR) PAC1-R is a neuropeptide pituitary adenylate cyclase activating polypeptide(PACAP)-preferring receptor that mediates the effective neuroprotective activity.Based on our previous data showing that doxycycline and minocycline work as the positive allosteric modulator (PAM) of RAC1-R,we used computer molecular docking and isothermal titration calorimetry assay to further determine the bindings of doxycycline/minocycline's derivatives including tetracycline/tigecycline with the N-terminal extracellular domain of PAC1-R (PAC1-EC1).Then the cAMP assay combined with the PAC1-R natural agonist PACAP27 was used to confirm the possible PAM roles of the small-molecule antibiotics. The results showed that tetracycline/tigecycline had significant lower affinity to PAC1-EC1 than doxycycline/minocycline, which was con sistent with their no n-positive allosteric modulation activity on PAC1-R.Furthermore, by comparing the key residues contributing to the PAM binding with the predicted allosteric site in PAC1-EC1,we characterized four motifs contributing to PAM binding in PAC1-EC1.The site-directed mutation results showed that ASN60 played the most important role in the PAM binding of the small-molecule antibiotics, while ASP116 played a sensitive marginal role in the PAM binding.These results not only help to explain the clinical and experimental neuroprotective effects of doxycycline/minocycline, but also help to characterize the PAM binding site in PAC1-EC1,which will promote the screening and characterization of novel small-molecule PAMs targeting PAC1-EC1 with drug development potency in nerve system disease. | Suqin Song Like Wang Junfeng Li Xiaoling Huang Rongjie Yu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，6： | 1 |
| 20 | An Intelligent Di- agnosis Method for Rotating Machinery Using Least Squares Mapping and a Fuzzy Neural Network 显示文摘 | LIKE CHEN PENG WANG SHIMING | 2012 | Sensors2012，12，： | 1 |
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