|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A microfluidic biosensor for rapid detection of Salmonella typhimurium based on magnetic separation,enzymatic catalysis and electrochemical impedance analysis显示文摘Rapid screening of foodborne pathogens is of great significance to ensure food safety.A microfluidic biosensor based on immunomagnetic separation,enzyme catalysis and electrochemical impedance analysis was developed for rapid and sensitive detection of S.typhimurium.First,the bacterial sample,the magnetic nanoparticles(MNPs)modified with capture antibodies,and the enzymatic probes modified with detection antibodies and glucose oxidase(GOx)were simultaneously injected into the microfluidic chip,followed by mixing and incubation to form MNP-bacteria-probe sandwich complexes.Then,glucose with high impedance was injected into the chip and catalyzed by the GOx on the complexes into hydrogen peroxide with high impedance and gluconic acid with low impedance,which was finally measured using the low-cost interdigitated microelectrode and the electrochemical impedance analyzer to determine the target bacteria.Under the optimal conditions,this biosensor could quantitatively detect S.typhimurium at the concentrations from 1.6×10^(2) CFU/m L to 1.6×10^(6) CFU/m L in 1 h with the low detection limit of 73 CFU/m L.Besides,this biosensor was demonstrated with good feasibility for practical applications by detecting the S.typhimurium spiked chicken meat samples. | Yingjia Liu Dong Jiang Siyuan Wang Gaozhe Cai Li Xue Yanbin Li Ming Liao Jianhan Lin | 2022 | Chinese Chemical Letters2022，33，6： | 3 |
| 2 | A portable viable Salmonella detection device based on microfluidic chip and recombinase aided amplification显示文摘creening of foodborne pathogens is important to prevent contaminated foods from their supply chains.n this study, a portable detection device was developed for rapid, sensitive and simple detection of viable almonella using a finger-actuated microfluidic chip and an improved recombinase aided amplification (RAA) assay. Improved propidium monoazide(PMAxx) was combined with RAA to enable this device to distinguish viable bacteria from dead ones. The modification of PMAxx into dead bacteria, the magnetic xtraction of nucleic acids from viable bacteria and the RAA detection of extracted nucleic acids were performed using the microfluidic chip on its supporting device by finger press-release operations. The fluorescent signal resulting from RAA amplification of the nucleic acids was collected using a USB camera nd analyzed using a self-developed smartphone App to quantitatively determine the bacterial concenration. This device could detect Salmonella typhimurium in spiked chicken meats from 1.3 × 10^(2) CFU/m L o 1.3 × 10^(7) CFU/m L in 2 h with a lower detection limit of 130 CFU/m L, and has shown its potential for on-site detection of foodborne pathogens. | Wuzhen Qi Siyuan Wang Lei Wang Xinge Xi Shangyi Wu Yanbin Li Ming Liao Jianhan Lin | 2023 | Chinese Chemical Letters2023，34，2： | 2 |
| 3 | Development and performance test of a laser controlled land levelingmachine 显示文摘 | Lin Jianhan Liu Gang Wang Maohua | 2007 | Agricultural Mechanization in Asia Africa and Latin America2007，38，2： | 1 |
| 4 | Devel- opment and performance test of a laser controlled land leveling machine 显示文摘 | Lin Jianhan Wang Maohua Lti Qingfei | 2007 | Agricultural Mechanization in A- sia Africa and Latin America2007，38，2： | 1 |
| 5 | Magnetorheological elastomer and smartphone enable microfluidic biosensing of foodborne pathogen显示文摘Rapid detection of foodborne pathogens is crucial to prevent the outbreaks of foodborne diseases.In this work,we proposed a novel microfluidic biosensor based on magnetorheological elastomer(MRE)and smartphone.First,micropump and microvalves were constructed by deforming the MRE under magnetic actuation and integrated into the microfluidic biosensor for fluidic control.Then,the micropump was used to deliver immune porous gold@platinum nanocatalysts(Au@PtNCs),bacterial sample,and immunomagnetic nanoparticles(MNPs)into a micromixer,where they were mixed,incubated and magnetically separated to obtain the Au@PtNC-bacteria-MNP complexes.After 3,3',5,5'-tetramethylbenzidine and hydrogen peroxide were injected and catalyzed by the Au@PtNCs,smartphone was used to measure the color of the catalysate for quantitative analysis of target bacteria.Under optimal conditions,this biosensor could detect Salmonella typhimurium quantitatively and automatically in 1 h with a linear detection range of 8.0×10^(1) CFU/mL to 8.0×10^(4) CFU/mL and a detection limit of 62 CFU/mL.The microfluidic biosensor was compact in size,simple to use,and efficient for detection,and might be used for in-field screening of foodborne pathogens to prevent food poisoning. | Gaozhe Cai Yuhe Wang Yingchao Zhang Lingyan Zheng Jianhan Lin | 2023 | Chinese Chemical Letters2023，34，8： | 0 |
| 6 | A lab-on-a-disc platform based on nickel nanowire net and smartphone imaging for rapid and automatic detection of foodborne bacteria显示文摘Foodborne pathogenic bacteria have been considered as a major risk factor for food safety. It is of great significance to carry out in-field screening of pathogenic bacteria to prevent the outbreaks of foodborne diseases. In this study, a portable lab-on-a-disc platform with a microfluidic disc was developed for rapid and automatic detection of Salmonella typhimurium using a nickel nanowire(Ni NW) net for effective separation of target bacteria, horseradish peroxidase nanoflowers(HRP NFs) for efficient amplification of biological signals, and a self-developed smartphone APP for accurate analysis of colorimetric images. First,the microfluidic disc was preloaded with reagents and samples and centrifuged to form one bacterial sample column, one immune Ni NW column, one HRP NF column, two washing buffer columns and one tetramethylbenzidine(TMB) column, which were separated by air gaps. Then, a rotatable magnetic field was specifically developed to assemble the Ni NWs into a net, which was automatically controlled by a stepped motor to successively pass through the sample column for specific capture of target bacteria, the HRP NF column for specific label of target bacteria, the washing columns for effective removal of sample background and non-specific binding NFs, and the TMB column for colorimetric determination of target bacteria. The color change of TMB from colorless to blue was finally analyzed using the smartphone APP to quantitatively determine the target bacteria. This lab-on-a-disc platform could detect Salmonella typhimurium from 5.6 × 10^(1) CFU/20 μL to 5.6 × 10^(5) CFU/20 μL in 1 h with a lower detection limit of 56 CFU/20 μL. The recovery of target bacteria in spiked chicken samples ranged from 97.5% to 101.8%. This portable platform integrating separation, labeling, washing, catalysis and detection onto a single disc is featured with automatic operation, fast reaction, and small size and has shown its potential for in-field detection of foodborne pathogens. | Xiaoting Huo Lei Wang Wuzhen Qi Na Rong Yingjia Liu Siyuan Wang Hong Duan Jianhan Lin | 2022 | Chinese Chemical Letters2022，33，4： | 0 |
| 7 | Development of automatic and efficient immuno-separator of foodborne pathogenic bacteria using magnetophoresis and magnetic mixing显示文摘In recent years,the outbreaks of foodborne diseases caused by pathogenic bacteria have made considerable economic losses and shown a threat to public health.The key to prevent and control these diseases is fast screening of pathogenic bacteria,which is usually performed with three procedures:sample collection,bacteria separation and bacteria detection.For sample collection,the national standard methods are often employed.For bacteria detection,currently available methods such as Polymerase Chain Reaction and Enzyme Linked Immuno-Sorbent Assay are often used.For bacteria separation,traditional methods such as filtration and centrifugation are not capable to specifically separate the target bacteria.However,food samples are very complicated and require efficient pretreatment for bacteria separation and concentration to achieve accurate and reliable results.The conventional immune magnetic separation method can be used to specifically separate the bacteria,but it still cannot meet the requirements for food sample pretreatment due to very low concentration of target bacteria in food.Therefore,this study developed an automatic and efficient immuno-separator of foodborne bacteria based on magnetophoresis and magnetic mixing,and E.coli O157:H7 was used as research model.A magnetic mixer was applied to facilitate the immunoreaction between the immune magnetic nanoparticles and the target bacteria cells,and a magnetophoretic separation tubing was utilized for magnetophoretic separation of the magnetic bacteria.Under the optimal mixing time of 20 min and the optimal flow rate of 50μL/min,the separation efficiency of E.coli O157:H7 could be more than 90%,showing that the developed immuno-separator is promising to be applied for efficient separation of foodborne bacteria and can be easily extended for separation of other biological targets by using their specific antibodies. | Yuhe Wang Gaozhe Cai Maohua Wang Jianhan Lin | 2019 | International Journal of Agricultural and Biological Engineering2019，12，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员