| 2 | Greenspace’s value orientations of ecosystem service and socioeconomic service in China显示文摘Background:Natural ecosystems provide necessary services for human beings,including ecosystem service values(ESVs)and socioeconomic service values(SSVs).The value orientations of ESVs and SSVs are mainly related to people’s interaction with nature.This study reclassified greenspace from a perspective of exposed and non-exposed greenspace based on the level of interaction by people and greenspace.We applied an expert questionnaire to survey the SSVs value orientations of forestland,grassland,wetland,and water bodies,and quantitatively compared the value orientations between the ESVs and SSVs of greenspace in China.Result:(1)The values of exposed greenspace were relatively far higher than nonexposed greenspace,as it had both ESVs and SSVs.(2)The forestland and grassland had relatively high ESVs and SSVs,and are the priority for both the exposed and non-exposed greenspace.(3)Wetland had relatively high ESVs but low SSVs,which was unpopular for exposed greenspace.(4)The ESVs and SSVs of water body were relatively balanced.Conclusion:Greenspace had both ESVs and SSVs when they are exposed to human.Our study provided an innovative perspective to explore the value orientations of greenspace,which provides an actionable scientific basis for greenspace planning,design and construction in human habitat. | Xia Yao Tao Lin Shoukai Sun Guoqin Zhang Hao Zhou Laurence Jones Wenhui Liu Yiyi Huang Meixia Lin Junmao Zhang Yuan Chena Hong Ye | 2022 | Ecosystem Health and Sustainability2022，8，1： | 1 |
| 3 | Selection and Validation of Reference Genes for Normalization of RT-qPCR Analysis in Developing or Abiotic-Stressed Tissues of Loquat (Eriobotrya japonica)显示文摘Loquat(Eriobotrya japonica Lindl.)is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components.Confirming suitable reference genes for a set of loquat samples before qRT-PCR experiments is essential for the accurate quantification of gene expression.In this study,eight candidate reference genes were selected from our previously published RNA-seq data,and primers for each candidate reference gene were designed and evaluated.The Cq values of the candidate reference genes were calculated by RT-qPCR in 31 different loquat samples,including 12 subgroups of developing or abiotic-stressed tissues.Different combinations of stable reference genes were screened according to a comprehensive rank,which was synthesized from the results of four algorithms,including the geNorm,NormFinder,BestKeeper andΔCt methods.The screened reference genes were verified by normalizing EjLGA1 in each subgroup.The obtained suitable combinations of reference genes for accurate normalization were GAPDH,EF1αand ACT for floral development;GAPDH,UBCE and ACT for fruit setting;EF1α,GAPDH and eIF2B for fruit ripening;ACT,EF1αand UBCE for leaves under heat stress;eIF2B,UBCE and EF1αfor leaves under freezing stress;EF1α,TUA and UBCE for leaves under salt stress;ACT,EF1αand eIF2B for immature pulp under freezing stress;ACT,UBCE and eIF2B for immature seeds under freezing stress;EF1α,eIF2B and UBCE for both immature pulp and seeds under freezing stress;UBCE,TUB and TUA for red-fleshed fruits under cold-storage stress;eIF2B,RPS3 and TUB for white-fleshed fruits under coldstorage stress;and eIF2B,UBCE and RPS3 for both red-and white-fleshed fruits under cold-storage stress.This study obtained different combinations of stable reference genes for accurate normalization in twelve subgroups of developing or abiotic-stressed tissues in loquat.To our knowledge,this is the first report to obtain stable reference genes for normalizing gene expression of abiotic-stressed tissues in E.japonica.The use of the three most stable reference genes could increase the reliability of future quantification experiments. | Shoukai Lin Shichang Xu Liyan Huang Fuxiang Qiu Yihong Zheng Qionghao Liu Shiwei Ma Bisha Wu Jincheng Wu | 2023 | Phyton-International Journal of Experimental Botany2023，92，4： | 0 |
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