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| 1 | Super-resolution dipole orientation mapping via polarization demodulation显示文摘Fluorescence polarization microscopy(FPM)aims to detect the dipole orientation of fluorophores and to resolve structural information for labeled organelles via wide-field or confocal microscopy.Conventional FPM often suffers from the presence of a large number of molecules within the diffraction-limited volume,with averaged fluorescence polarization collected from a group of dipoles with different orientations.Here,we apply sparse deconvolution and least-squares estimation to fluorescence polarization modulation data and demonstrate a super-resolution dipole orientation mapping(SDOM)method that resolves the effective dipole orientation from a much smaller number of fluorescent molecules within a sub-diffraction focal area.We further apply this method to resolve structural details in both fixed and live cells.For the first time,we show that different borders of a dendritic spine neck exhibit a heterogeneous distribution of dipole orientation.Furthermore,we illustrate that the dipole is always perpendicular to the direction of actin filaments in mammalian kidney cells and radially distributed in the hourglass structure of the septin protein under specific labelling.The accuracy of the dipole orientation can be further mapped using the orientation uniform factor,which shows the superiority of SDOM compared with its wide-field counterpart as the number of molecules is decreased within the smaller focal area.Using the inherent feature of the orientation dipole,the SDOM technique,with its fast imaging speed(at sub-second scale),can be applied to a broad range of fluorescently labeled biological systems to simultaneously resolve the valuable dipole orientation information with super-resolution imaging. | Karl Zhanghao Long Chen Xu-San Yang Miao-Yan Wang Zhen-Li Jing Hong-Bin Han Michael Q Zhang Dayong Jin Jun-Tao Gao Peng Xi | 2016 | Light(Science & Applications)2016，5，1： | 11 |
| 2 | Cytokines as biomarkers of inflammatory response after open versus endovascular repair of abdominal aortic aneurysms: a systematic review显示文摘一个腹的大动脉的动脉瘤(AAA ) 的修理是与有煽动性的串联的随后的激活的神经质、新陈代谢的压力相关的反应有关的一个高风险的外科的过程。与开的修理相对照(或) , endovascular 大动脉的动脉瘤修理(EVAR ) 由为更少提供广泛的切口,解剖,和织物操作似乎是手术后的压力到减少。然而,这些有益的效果可以是由代谢物在用在 endovascular 的导管的血栓的 intra 钠操作期间修理的 cytokines 和 arachidonic 酸的版本的偏移量,导致全身的煽动性的反应(先生) ,它是临床上叫的培植以后的症候群。在我们比较了的这系统的评论或与 EVAR,以 post-interventional,从改变导致传播 cytokine 的煽动性的反应铺平。我们寻求了在 EVAR 以后关于培植以后的症候群总结所有最近的证据。我们寻找了为临床的研究作为煽动性的反应的部分在 cytokines 的版本上报导在以后的 Medline (PubMed ) , ClinicalTrials.gov 和 Cochrane 图书馆开 / 常规并且 AAA 的 endovascular 修理。我们鉴别 17 学习检验 cytokine 层次在以后或对 EVAR。或似乎特别地比 EVAR 与一位更大的先生被联系,由增加的 cytokine 层次证实了 IL-6 和 IL-8 而 IL-1, IL-10 和 TNF- 出现了,冲突在二之间的结果或没有差别组织。聚酯 endografts 看起来断然在 EVAR 以后与培植以后的症候群的发生被相关。未来大未来的研究被保证位于在 surgical 以后的 cytokine 相互作用下面描出的机制煽动性的反应背景。 | Diamantis I TSILIMIGRAS Fragiska SIGALA Georgios KARAOLANIS Ioannis NTANASIS-STATHOPOULOS Eleftherios SPARTALIS Michael SPARTALIS Nikolaos PATELIS Alexandros PAPALAMPROS Chandler LONG Demetrios MORIS | 2018 | Acta Pharmacologica Sinica2018，39，7： | 5 |
| 3 | Accelerated restructuring in rural China fueled by ‘increasing vs. decreasing balance’ land-use policy for dealing with hollowed villages显示文摘 | Hualou Long Yurui Li Yansui Liu Michael Woods Jian Zou | 2011 | Land Use Policy2011，，1： | 4 |
| 4 | Risk of Melanoma and Nonmelanoma Skin Cancer Among Patients With Inflammatory Bowel Disease显示文摘 | Millie D. Long Christopher F. Martin Clare A. Pipkin Hans H. Herfarth Robert S. Sandler Michael D. Kappelman | 2012 | Gastroenterology2012，，2： | 4 |
| 5 | Defective T wave combined with incomplete right bundle branch block: a new electrocardiographic index for diagnosing atrial septal defect显示文摘 | WANG Mu-xuan WU Gui-fu GU Jing-li LI Li LU Kun YANG Da CHEN Long ZHANG Xi LUO Fu-tian Andrew D. Michaels MA Hong | 2012 | Chinese Medical Journal2012，，6： | 4 |
| 6 | Four-sample lactose hydrogen breath test for diagnosis of lactose malabsorption in irritable bowel syndrome patients with diarrhea显示文摘AIM: To validate 4-sample lactose hydrogen breath testing(4SLHBT) compared to standard 13-sample LHBT in the clinical setting.METHODS: Irritable bowel syndrome patients with diarrhea(IBS-D) and healthy volunteers(HVs) were enrolled and received a 10 g, 20 g, or 40 g doselactose hydrogen breath test(LHBT) in a randomized, double-blinded, controlled trial. The lactase gene promoter region was sequenced. Breath samples and symptoms were acquired at baseline and every 15 min for 3 h(13 measurements). The detection rates of lactose malabsorption(LM) and lactose intolerance(LI) for a 4SLHBT that acquired four measurements at 0, 90, 120, and 180 min from the same data set were compared with the results of standard LHBT.RESULTS: Sixty IBS-D patients and 60 HVs were studied. The genotype in all participants was C/C-13910. LM and LI detection rates increased with lactose dose from 10 g, 20 g to 40 g in both groups(P < 0.001). 4SLHBT showed excellent diagnostic concordance with standard LHBT(97%-100%, Kappa 0.815-0.942) with high sensitivity(90%-100%) and specificity(100%) at all three lactose doses in both groups.CONCLUSION: Reducing the number of measurements from 13 to 4 samples did not significantly impact on the accuracy of LHBT in health and IBS-D. 4SLHBT is a valid test for assessment of LM and LI in clinical practice. | Jian-Feng Yang Mark Fox Hua Chu Xia Zheng Yan-Qin Long Daniel Pohl Michael Fried Ning Dai | 2015 | World Journal of Gastroenterology2015，21，24： | 4 |
| 7 | Increased Risk for Non-Melanoma Skin Cancer in Patients With Inflammatory Bowel Disease显示文摘 | Millie D. Long Hans H. Herfarth Clare A. Pipkin Carol Q. Porter Robert S. Sandler Michael D. Kappelman | 2010 | Clinical Gastroenterology and Hepatology2010，，3： | 3 |
| 8 | Sleep Disturbance and Risk of Active Disease in Patients With Crohn’s Disease and Ulcerative Colitis显示文摘 | Ashwin N. Ananthakrishnan Millie D. Long Christopher F. Martin Robert S. Sandler Michael D. Kappelman | 2013 | Clinical Gastroenterology and Hepatology2013，，： | 3 |
| 9 | 满足安防和监控市场中视频分析需求的处理器显示文摘本文将探讨新兴且不断发展的安防工业,以及处理技术怎样在这些最新发展中扮演关键角色。 | Michael Long | 2013 | 电子产品世界2013，20，7： | 3 |
| 10 | Mesencephalic astrocyte-derived neurotrophic factor ameliorates steatosis in HepG2 cells by regulating hepatic lipid metabolism显示文摘BACKGROUND Nonalcoholic fatty liver disease (NAFLD) is a global metabolism-associated liver disease.Mesencephalic astrocyte-derived neurotrophic factor (MANF) is a newly discovered secreted protein that is involved in metabolic homeostasis.However,much remains to be discovered about its function in hepatic lipid metabolism;thus,we assessed whether MANF could regulate hepatic metabolism.AIM To establish in vivo and in vitro NAFLD models to explore the role of MANF in hepatic lipid metabolism.METHODS HepG2 cells treated with free fatty acids (FFAs) and ob/ob mice were used as NAFLD models.Liver tissues collected from wild type and ob/ob mice were used to detect MANF expression.Cells were treated with FFAs for different durations.Moreover,we used lentiviral constructs to establish overexpression and knockdown cell models in order to interfere with MANF expression levels and observe whether MANF influences hepatic steatosis.Western blot analysis and quantitative real-time PCR were used to detect protein and gene expression,and oil red O staining was used to visualize intracellular lipid droplets.RESULTS Hepatic MANF protein and mRNA expression in wild type mice were 10-fold and 2-fold higher,respectively,than those in ob/ob mice.The MANF protein was temporarily increased by 1.3-fold after stimulation with FFAs for 24 h and gradually decreased to 0.66-fold that of the control at the 72 h time point in HepG2 cells.MANF deficiency upregulated the expression of genes involved infatty acid synthesis,cholesterol synthesis,and fatty acid uptake and aggravated HepG2 cell steatosis,while MANF overexpression inhibited fatty acid synthesis and uptake and cholesterol synthesis,and rescued HepG2 cells from FFAsinduced steatosis.Furthermore,a significant decrease in triglyceride levels was observed in the MANF overexpression group compared with the control group(0.4288±0.0081 mmol/g vs 0.3746±0.0121 mmol/g,P <0.05) upon FF As treatment.There was also a 17%decrease in intracellular total cholesterol levels between the MANF overexpression group and the control group (0.1301±0.0059mmol/g vs 0.1088±0.0009 mmol/g,P <0.05) upon FF As treatment.Moreover,MANF suppressed lipid deposition in HepG2 cells.CONCLUSION Our findings indicate that MANF improves the phenotype of liver cell steatosis and may be a potential therapeutic target in hepatic steatosis processes. | Miao He Cong Wang Xiao-Hong Long Jia-Jia Peng Dong-Fang Liu Gang-Yi Yang Michael D Jensen Li-Li Zhang | 2020 | World Journal of Gastroenterology2020，26，10： | 3 |
| 11 | Linguistic and Conversational Adjustments to Non-Native Speakers显示文摘 | Michael H. Long | 1983 | Studies in Second Language Acquisition1983，，2： | 3 |
| 12 | Database for Retaining Wall and Ground Movements due to Deep Excavations显示文摘 | Michael Long | 2001 | Journal of Geotechnical and Geoenvironmental Engineering2001，，3： | 2 |
| 13 | The insect-killing bacterium Photorhabdus luminescens has the lowest mutation rate among bacteria显示文摘Mutation is a primary source of genetic variation that is used to power evolution.Many studies,however,have shown that most mutations are deleterious and,as a result,extremely low mutation rates might be benefcial for survival.Using a mutation accumulation experiment,an unbiased method for mutation study,we found an extremely low base-substitution mutation rate of 5.94×10^(-11) per nucleotide site per cell division(95%Poisson confdence intervals:4.65×10^(-11),7.48×10^(-11))and indel mutation rate of 8.25×10^(-12) per site per cell division(95%confdence intervals:3.96×10^(-12),1.52×10^(-11))in the bacterium Photorhabdus luminescens ATCC29999.The mutations are strongly A/T-biased with a mutation bias of 10.28 in the A/T direction.It has been hypothesized that the ability for selection to lower mutation rates is inversely proportional to the efective population size(drift-barrier hypothesis)and we found that the efective population size of this bacterium is signifcantly greater than most other bacteria.This fnding further decreases the lower-bounds of bacterial mutation rates and provides evidence that extreme levels of replication fdelity can evolve within organisms that maintain large efective population sizes. | Jiao Pan Emily Williams Way Sung Michael Lynch Hongan Long | 2021 | Marine Life Science & Technology2021，3，1： | 2 |
| 14 | DNA barcoding of East Asian Amentotaxus (Taxaceae): Potential new species and implications for conservation显示文摘DNA barcoding 是为用标准化 genomic DNA 碎片的种类鉴定的一个有用工具。类 Amentotaxus,由五或六种组成,被限制到华南,东北印度,老挝,和越南。所有种类是被估计了全球性或全国地威胁了。然而,关于包含的种类的数字有无常,特别在南部的中国,北越南,和老挝的边阶区域。我们选择了五 DNA barcodes (rbcL, matK, trnH-psbA, trnL-F,和内部抄录分隔符(它的)) 在这个类评估他们的辨别能力,并且调查 Amentotaxus 的当前的分类。我们的结果显示所有选择 barcoding 区域为 PCR 显示出普遍性的高水平并且定序。当六种被认出时,原子 ribosomal DNA 区域它叶绿体 DNA 区域 trnL-F 在他们的自己上使用了的 and 提供了最高的鉴定成功(60%) 。也包括了它的 or trnL-F 的二 barcode 联合有一样的种类辨别能力。用另外的 barcodes 的联合没改进种类鉴定成功。五种什么时候仅仅被认出,与 A。hatuyenensis T. H。当作 A 的一个同义词的 Nguyen。yunnanensis H.L. 李,辨别率升起到 100% 。我们的结果也显示从云南省,中国,老挝人的蔡省,越南,和老挝的最近的集合可以代表潜在的新种。从这研究的调查结果将为为在公民和 trans 边界区域的威胁的 Amentotaxus 种类的适当保存策略的明确的表达是很有用的。 | Lian-Ming Gao Yan Li Loc Ke Phan Li-Jun Yan Philip Thomas Long Ke Phan Michael Mbller De-Zhu Li | 2017 | Journal of Systematics and Evolution2017，55，1： | 2 |
| 15 | Accelerated restructuring in rural China fueled by ‘increasing vs. decreasing balance’ land-use policy for dealing with hollowed villages显示文摘 | Hualou Long Yurui Li Yansui Liu Michael Woods Jian Zou | 2011 | Land Use Policy2011，，1： | 2 |
| 16 | Graphene-loaded metal wire grating for deep and broadband THz modulation in total internal reflection geometry显示文摘We employed a metallic wire grating loaded with graphene and operating in total internal reflection (TIR) geometry to realize deep and broadband THz modulation. The non-resonant field enhancement effect of the evanescent wave in TIR geometry and in the subwavelength wire grating was combined to demonstrate a ~77% modulation depth (MD) in the frequency range of 0.2–1.4 THz. This MD, achieved electrically with a SiO_2∕Si gated graphene device, was 4.5 times higher than that of the device without a metal grating in transmission geometry. By optimizing the parameters of the metallic wire grating, the required sheet conductivity of graphene for deep modulation was lowered to 0.87 mS. This work has potential applications in THz communication and real-time THz imaging. | YIWEN SUN RICCARDO DEGLJINNOCENTI DAVID A.RITCHIE HARVEY E.BEERE LONG XIAO MICHAEL RUGGIERO J.AXEL ZEITLER RAYKO I.STANTCHEV DANNI CHEN ZHENGCHUN PENG EMMA MACPHERSON XUDONG LIU | 2018 | Photonics Research2018，6，12： | 2 |
| 17 | Database for Retaining Wall and Ground Movements due to Deep Excavations显示文摘 | Michael Long | 2001 | Journal of Geotechnical and Geoenvironmental Engineering2001，，3： | 2 |
| 18 | 中国国内发展与外交互动关系研究——以上合组织为例显示文摘研究中国外交的学者经常忽略一个巨大事实:中国外交经常和国内发展目标直接相关。在中国国内发展和外交关系中,'西部大开发'政策是当代中国最显著的案例之一。自1999年'西部大开发'政策实施以来,中国很积极地接触中亚邻国,通过改善关系,深入合作,中亚国家和中国西北部经济已经实现快速发展。本文以'西部大开发'和上合组织之间关系的案例试图证明中国国内发展与中国外交的关系。本文有三个主要部分:第一,简单解释当代中国中亚关系背景和中国西部大开发的制度性困难。第二,阐述中国中亚关系改善的主要障碍,中国如何改善上述障碍以及中国西部和中亚的经济一体化。第三,反驳一系列西方对上合组织的误解。 | Michael Long | 2013 | 理论界2013，，7： | 2 |
| 19 | The accurate determination of potassium and calcium using isotope dilution inductively coupled 'cold' plasma mass spectrometry显示文摘 | Karen E Murphy Stephen E Long Michael S Rearick | 2002 | J Anal2002，17，46： | 1 |
| 20 | Structureactivity relationship studies of salubrinal lead to its active biotinylated derivative显示文摘 | Long Kai Boyee Michael Lin He | 2005 | Bioorganic & Medicinal Chemistry Letters2005，15，17： | 1 |
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