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| 1 | Histone Deacetylase AtSRT1 Links Metabolic Flux and Stress Response in Arabidopsis显示文摘怎么种新陈代谢的流动,改变基因表示优化植物生长,对应力的反应仍然保持大部分不清楚。这里,我们报导 Arabidopsis thaliana NAD +-dependent histone deacetylase AtSRT1 否定地调整植物公差到应力和 glycolysis,但是刺激 mitochondrial 呼吸。我们发现 AtSRT1 与 Arabidopsis cMyc 有约束力的蛋白质 1 交往(AtMBP-1 ) ,一个 transcriptional 抑压者由 cytosolic glycolytic enolase 基因 LOS2/ENO2 的其他的翻译生产了。我们证明 AtSRT1 能与 AtMBP-1 目标 LOS2/ENO2 和 STZ/ZAT10 的染色质联系,哪个编码关键压力管理者,并且在这些基因减少 H3K9ac 层次镇压他们的抄写。AtSRT1 和 AtMBP-1 的 Overexpression 在 glycolytic 基因的表示上有 synergistic 效果, glycolytic 酶的活动,和 mitochondrial 呼吸。而且,我们发现 AtMBP-1 是 lysine-acetylated 并且对 proteasomal 蛋白质降级脆弱,当 AtSRT1 能移开它的离氨酸 acetylation 并且显著地提高它的稳定性在时 ? vivo。一起拿,这些结果显示 AtSRT1 在 Arabidopsis 由 epigenetic 规定和 AtMBP-1 transcriptional 活动的调整调整主要新陈代谢和压力反应。 | Xiaoyun Liu Wei Wei Wenjun Zhu Lufang Su Zeyang Xiong Man Zhou Yu Zheng and Dao-Xiu Zhou | 2017 | Molecular Plant2017，10，12： | 6 |
| 2 | Histone Deacetylase HDA9 and WRKY53 Transcription Factor Are Mutual Antagonists in Regulation of Plant Stress Response显示文摘Epigenetic regulation of gene expression is important for plant adaptation to environm ental changes.Previous results showed that Arabidopsis RPD3-like histone deacetylase HDA9 is known to function in repressing plant response to stress in Arabidopsis.However,how HDA9 targets to specific chromatin loci and controls gene expression netw orks involved in plant response to stress remains largely unclear.Here,we show that HDA9 represses stress tolerance response by interacting with and regulating the DNA binding and transcriptional activity of WRKY53,which functions as a high-hierarchy positive regulator of stress response.We found that WRKY53 is post-translationally modified by lysine acetylation at multiple sites,some of which are removed by HDA9,resulting in inhibition of WRKY53 transcription activity.Conversely,WRKY53 negatively regulates HDA9 histone deacetylase activity.Collectively,our results indicate that HDA9 and WRK53 are reciprocal negative regulators of each other's activities,illustrating how the functional interplay between a chromatin regulator and a transcription factor regulates stress tolerance in plants. | Yu Zheng Jingyu Ge Chun Bao Wenwen Chang Jingjing Liu Jingjie Shao Xiaoyun Liu Lufang Su Lei Pan Dao-Xiu Zhou | 2020 | Molecular Plant2020，13，4： | 6 |
| 3 | 线粒体融合蛋白与心血管疾病显示文摘心血管疾病(CVD)发生是一种多因素综合症候群,在全球范围内,是威胁生命的头号杀手,并且发病率随年龄的增长呈稳定上升趋势。虽然诊断和治疗已经取得了进步,但是CVD的发病率和死亡率仍然很高,部分原因归究于对其病理机制理解不清。心脏的首要功能是为器官供血,这就需要持续而大量的ATP能量供应。因此线粒体在心肌细胞中广泛丰富分布。正常的线粒体功能的维持依赖于其完整性和相互作用,并且受到线粒体融合蛋白和分裂蛋白的调控。该文简要综述了线粒体融合蛋白及其改变,特别是线粒体融合蛋白2异常在CVD发生发展中的作用。了解CVD中线粒体融合蛋白的作用对于增进知识以及发展新的治疗策略具有重要的意义。 | KahYong Goh Lufang Zhou 练桂丽 | 2014 | 中华高血压杂志2014，22，10： | 0 |
| 4 | High-level expression of recombinant IgG1 by CHO K1 platform显示文摘中国仓鼠卵巢(CHO K1 ) 房间被用来由在这研究优化生产房间线的构造的站台表示指向的反癌症 monoclonal 抗体。支持者 CHO K1 首先在化学定义媒介被使适应暂停文化。然后,夫酸安合成酶(GS ) 向量被使用构造单个 plasmid 到 overexpress monoclonal 抗体 IgG1。波斯特 transfection,房间水池的生产被没有夫酸安的选择和扩大用蛋氨酸 sulfoximine 的各种各样的集中优化。CHO K1/IgG1 的最好的房间水池被用来用克隆的限制冲淡屏蔽最高的单个克隆。最后, 780 mg/L 的高 IgG1 生产从一种批文化被获得。这研究证明高生产房间的构造排队,从基因到克隆,能在六个月以内被完成,基因扩大极大地改进了蛋白质生产。 | Ningning Xu Jianfa Ou Al-Karim (AI) Gilani Lufang Zhou Margaret Liu | 2015 | Frontiers of Chemical Science and Engineering2015，9，3： | 0 |
| 5 | Research on the influence of outdoor trees on natural ventilation performance of an academic building显示文摘Planting trees around buildings has always been used as one of the most common viable landscaping strategies in schools.However,the impact of trees on natural ventilation inside the building has been neglected without investigation.The emphasis of this study related to the impact of outdoor trees around the academic building on indoor ventilation.Numerical simulations of the indoor wind environment of the academic building affected by trees were performed utilizing the k-εmodel with additional source terms.The numerical model was also validated by measured data.Two kinds of trees were selected,they are camphor and metasequoia.Camphor is a kind of broad-leaved tree and metasequoia is a kind of coniferous tree.26 simulation cases with six different tree canopy spacings were conducted.These results showed that the outdoor trees had great influences on the natural ventilation performance of the academic building.Compared with the case without the trees,the highest decrement in ventilation flow rate could be up to 31.97%in this study.For the cases of classrooms with horizontal distribution,the ages of air of the classrooms became fresher with the increase of the canopy spacing.While for the cases of classrooms with vertical distribution,the canopy spacing had fewer effects on the natural ventilation performances.It was also found that the blocking effects of camphor on indoor ventilation were higher than that of metasequoia.The average ventilation flow rate in cases with metasequoia was increased by 14.89%compared to the cases with camphor.This study could provide guidance for the layout design of trees around the building. | Shengnan Niu Xiaodong Liu Annan Wang Lufang Chen Xin Zhou Xing Shi Liu Yang Xing Jin | 2023 | Building Simulation2023，16，6： | 0 |
| 6 | Establishment of a new molecular model for mercury determination verified by single crystal X-ray diffraction,spectroscopic analysis and biological potentials显示文摘A wide variety of molecular probes have been developed for real-time analysis,but most of organic fluorophores possess small Stokes shifts and self-absorption or inner filter effect that could not be avoided.In this study,a new dicyanoisophorone-based derivative(E)-0-(4-(2-(3-(dicyanomethylene)-5,5-dimethylcyclohex-1-en-1-yl)vinyl)phenyl)diphenylphosphinothioate(λ_(ex)=405 nm,X_(em)=551 nm,denoted as ICM-S) with strong push-pull electron effect has been afforded and it exhibits red shift for absorption from 407 nm to 426 nm with distinct color change from pale yellow to deep yellow upon exposure to Hg~(2+).Moreover,an easily distinguishable fluorescence color change follows the route from green,yellow to red in the presence of Hg~(2+) over the range of 0-90 μmol/L(detection limit=137 nmol/L)can be observed by the naked eye under a UV lamp irradiation.Chlorodiphenylphosphine and sublimedsulfur are incorpo rated as re s ponsive sites and P-O bond has been cleaved upon the addition of mercu ry ions.During the recognition process,such dicyanoisophorone dye(ICM-S) has been evolved to 2-(3-(4-hydroxystyryl)-5,5-dimethylcyclohex-2-enylidene) malononitrile(ICM-OH).Clear evidences in the chemical processes can be identified via single crystal X-ray diffraction,spectroscopic analysis,photophysical studies and titration experiments.With the aim of exploring its potential in biological systems,its in vitro responses to Hg~(2+) have been evaluated in 293 T cells and the effectiveness in zebrafish model has also been verified. | Jiapei Gu Feifan Zhang Ziman Zheng Xiangqian Li Runxuan Deng Zhan Zhou Lufang Ma Wanqiang Liu Qianming Wang | 2021 | Chinese Chemical Letters2021，32，1： | 0 |
| 7 | Early rehabilitation and neuroprotective drug therapy outcomes in elderly patients with acute stroke显示文摘Sixty elderly patients, who suffered from acute stroke and were admitted within a 1-year period to the Department of Geriatrics in the First Affiliated Hospital of School of Medicine, Zhejiang University, China, underwent early rehabilitation in combination with neuroprotective drug therapy. Limb movement, cognitive functions and daily life self-care ability in elderly patients upon admission and discharge were assessed using the Hunt-Hess scale, functional independence measures and mini-mental state examination. The mean duration of hospital stay among the 60 patients was 35 days. Upon discharge, 42 (75%) of the patients exhibited cognitive impairment to varying degrees, and 25 (45%) of the 56 stroke patients who underwent rehabilitation evaluation attained independence in daily living activities, 11 (20%) required intermittent supervision, and 20 (36%) required 24-hour constant supervision during performance of these activities. Results demonstrated that early rehabilitation treatment in combination with neuroprotective therapy for acute stroke was effective. | Yue Chen Lufang Chen Yiqing Tao Feixue Zhou Chunlan Cui Shichao Liu | 2011 | Neural Regeneration Research2011，6，11： | 0 |
| 8 | Serologic study on the outbreak of acute upper respiratory tract infections caused by adenovirus 3显示文摘From April to June,2004,an outbreak of acute upper respiratory tract infections(AURTI)occurred in the north area of Jiangsu Province,China.Twenty throat swabs were collected with 13 of them presenting an adenovirus(Ad)-like cytopathogenic effect on HEp-2.These were verified as Ad by the electron microscope,direct immunofluorescence assay and Ad primer-mediated PCR.Moreover,they were identified as adenovirus type 3(Ad3)by type-specific PCR and sequencing of the amplification products.Subsequent serologic studies were carried out to finally diagnose and document the outbreak.The neutralization test of paired serum of six in nine cases show obviously increased antibodies titers.The positive rate of IgM,IgG and recovery phase neutralization antibodies of the cases were 3.7%,44.4%and 59.5%respectively while those of the controls were 0%,8.3%and 33.3%respectively.The P values of Chi-Square were 0.510,0.018 and 0.226 respectively.The concordance between IgG detected by ELISA and neutralization anti bodies detected by the neutralization test was 61.4%and the P value of Kappa was 0.070.By the serologic study,we can definitively diagnose that this outbreak of acute respiratory infections was caused by Adenovirus 3. | JIANG Lufang JU Liwen JIANG Renjie LIN Yuzun ZHOU Liandi YU Shunzhang JIANG Qingwu | 2007 | Frontiers of Medicine2007，1，2： | 0 |
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