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| 1 | Estimation of internal nutrient release in large shallow Lake Taihu, China显示文摘Based on field investigation of wave, sediment suspension and the changes in nutrient concentration of the water column in Lake Taihu, China, we proposed two release models to quantify nutrient release under static and dynamic conditions, respectively. Under static conditions, nutrient release from sediments to the overlying water mainly depends on chemical diffusion induced by concentration gradient, in which the nutrient release is controlled by the temperature, dissolved oxygen concentration in the sediment-water interface, oxidation-reduction potential and the concentration difference between porewater and overlying water. Under dynamic condition (or disturbed condition),both dissolved and particulate nutrients in sediments are released into the water column because of wind-induced sediment suspension. The amount of nutrient release under dynamic conditions is larger than that under the static condition. The release of dissolved nutrients, however, does not increase because the wind induced turbulence made oxidation of metallic elements such as Fe (ferric iron), Mn which are capable of precipitating soluble reactive phosphate (SRP). Under dynamic conditions, therefore, the release of total phosphorus (TP) increases dramatically but the release of SRP is close to those under static conditions. In sediments of Lake Taihu, high Fe content leads to a high ratio of Fe to P contents in sediments (Fe:P ratio). Under dynamic conditions, therefore, nutrient release is controlled by the intensity of disturbance, sediment consolidation and nutrient content in sediments.As for dissolved nutrients, especially SRP, the release is also controlled by the intensity of dynamic re-oxidation, Fe content in sediments and nutrient concentration gradient between porewater and overlying water. Based on these two release modes, the release flux in Lake Taihu has been estimated. In the static condition (i.e. laboratory experimental condition), total release of NH4+-N for whole lake is ca. 10,000 ton/a, and PO43--P is ca. 900 ton/a. In the dynamic condition, nutrient release following sediment suspension was estimated according to three different intensities of wind forcing which were defined as 'calm' (wind speed is less than 2 m/s), 'gentle' (wind speed is greater than 2 m/s and less than 6 m/s) and 'gust' (wind speed is greater than 6 m/s). The release rate in the condition of 'calm' was estimated in terms of the nutrient release in the laboratory experimental static condition; whereas the release rate in conditions of 'gentle' and 'gust' was estimated in terms of measurement during sediment resuspension conducted in flume experiments. With the observation of wind velocity and frequency in 2001, each type of wind forcing took the frequency of 12%, 82% and 6% for 'calm', 'gentle' and 'gust', respectively. The yearly release of nitrogen was 81,000 ton and phosphorus was 21,000 ton, which is about 2-6 folds of annual external loading, respectively. | QIN Boqiang, ZHU Guangwei, ZHANG Lu, LUO Liancong, GAO Guang & GU Binhe Nanjing Institute of Geography & Limnology, Chinese Academy of Sciences, Nanjing 210008, China Everglades Department, South Florida Water Management District, 3301 Gun Club Road, West Palm Beach, FL 33406, USA | 2006 | Science China Earth Sciences2006，49，z1： | 37 |
| 2 | Efficacy and safety of a novel anti-HER2 therapeutic antibody RC48 in patients with HER2-overexpressing,locally advanced or metastatic gastric or gastroesophageal junction cancer:a single-arm phase II study显示文摘Background:Current treatment options for human epidermal growth factor receptor 2(HER2)-overexpressing gastric cancer at third-line have shown limited clinical benefit.Further,there is no specific treatment for HER2 immunohistochemistry(IHC)2+and fluorescence in-situ hybridization-negative patients.Here,we report the efficacy and safety of a novel anti-HER2 antibody RC48 for patients with HER2-overexpressing,advanced gastric or gastroesophageal junction cancer.Methods:Patients with HER2-overexpressing(IHC 2+or 3+),locally advanced or metastatic gastric or gastroesophageal junction cancer who were under at least second-line therapy were eligible and received RC482.5 mg/kg alone every 2 weeks.The primary endpoint was the objective response rate(ORR)assessed by an independent review committee.Secondary endpoints included progressionfree survival(PFS),overall survival(OS),duration of response,time to progression,disease control rate,and safety.Results:Of 179 patients screened,125 were eligible and received RC48 treatment.The ORR was 24.8%(95%confidence interval[CI]:17.5%-33.3%).The median PFS and OS were 4.1 months(95%CI:3.7-4.9 months)and 7.9 months(95%CI:6.7-9.9 months),respectively.The most frequently reported adverse events were decreased white blood cell count(53.6%),asthenia(53.6%),hair loss(53.6%),decreased neutrophil count(52.0%),anemia(49.6%),and increased aspartate aminotransferase level(43.2%).Serious adverse events(SAEs)occurred in 45(36.0%)patients,and RC48-related SAEs were mainly decreased neutrophil count(3.2%).Seven patients had adverse events that led to death were not RC48-related.Conclusions:RC48 showed promising activity with manageable safety,suggesting potential application in patients with HER2-overexpressing,advanced gastric or gastroesophageal junction cancer who have previously received at least two lines of chemotherapy. | Zhi Peng Tianshu Liu Jia Wei Airong Wang Yifu He Liuzhong Yang Xizhi Zhang Nanfeng Fan Suxia Luo Zhen Li Kangsheng Gu Jianwei Lu Jianming Xu Qingxia Fan Ruihua Xu Liangming Zhang Enxiao Li Yuping Sun Guohua Yu Chunmei Bai Yong Liu Jiangzheng Zeng Jieer Ying Xinjun Liang Nong Xu Chao Gao Yongqian Shu Dong Ma Guanghai Dai Shengmian Li Ting Deng Yuehong Cui Jianmin Fang Yi Ba Lin Shen | 2021 | Cancer Communications2021，41，11： | 29 |
| 3 | An-2 Encodes a Cytokinin Synthesis Enzyme that Regulates Awn Length and Grain Production in Rice显示文摘大量词法、生理的特点在驯服下面在栽培米饭 Oryza sativa 和野米饭 Oryza rufipogon 之间变化了。这里,我们报导 An-2 基因克隆,编码象蛋白质 6 一样的孤独的盖伊(OsLOGL6 ) ,它在 O 催化细胞激动素合成的最后的步。rufipogon。在 theawnless indica Guangluai 4 的基因背景怀有 An-2 的野等位基因的 near-isogenic 线证明 An-2 由由每植物每圆锥花序和 tillers 减少谷物提高房间分割,而是减少谷物生产支持芒延伸。我们表明在 An-2 地点的一个基因变化在减少芒长度并且在驯养的米饭增加 tiller 和谷物数字上有大影响。基因 expressionpatterns 的分析建议 An-1 调整芒的形成初发,并且 An-2 支持芒延伸。在栽培米饭的 theAn-2 地点的核苷酸差异被发现显著地与野米饭的相比被减少,建议 An-2 地点受到人工的选择。我们因此在 An-2 的基因变化的选择由于减少的芒长度并且增加的 proposethat 在栽培米饭的谷物产量。 | Benguo Gu Taoying Zhou Jianghong Luo Hui Liu Yongchun Wang Yingying Shangguan Jingjie Zhu Yan Li Tao Sang Zixuan Wang Bin Han | 2015 | Molecular Plant2015，8，11： | 22 |
| 4 | A novel gene delivery system targeting cells expressing VEGF receptors显示文摘Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues. | LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( National Labomtory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032,China)(National Laboratory of | 1999 | Cell Research1999，9，1： | 22 |
| 5 | Effect of Glycyrrhiza uralensis Fisch polysaccharide on growth performance and immunologic function in mice in Ural City,Xinjiang显示文摘Objective:To discuss the effect of Glycyrrhiza uralensis(G.uralensis) Fisch polysaccharide on growth performance and immunologic function in mice in Ural City,Xinjiang and to provide important data supporting the application of Glycyrrhiza polysaccharide.Methods:A total of100 Kunming mice aged 3 weeks old were randomly divided into 5 groups with 20 mice in each group(10 were females and 10 were males).About 0.5 mL normal saline was given to the mice of control group every day and 0.5 mL G.uralensis Fisch polysaccharide was given to the mice of other groups at the concentration of 1,20,50 and 100 mg/mL respectively.The growth performance(average body weight,average daily feed intake and feed efficiency),immune organ indexes(spleen index and thymus index) and immunologic function(serum IL-2,CD4^+/CD8^+ and the activity of NK cells) of mice in each group were detected continuously.Results:The average body weight,feed efficiency,serum IL-2,CD4^+/CD8^+ and the activity of NK cells of mice were increased with the increase of administrated time after administrating G.uralensis Fisch polysaccharide and were reached up the largest level on Day 28.At the same time,each index was proportional to the given dose and was significantly higher than those of control group and reached up the largest level at the administrated dose of 100 mg/mL.After administrating G.uralensis Fisch polysaccharide,the spleen index and thymus index of mice were increased with the increase of administrated dose and the spleen index and thymus index of mice administrated with the dose of 100 mg/mL were maximum which was more than 1.51 times and 1.43 times of that in control group respectively and the comparative differences showed statistical significance(P<0.05).The average daily feed intake of mice in each group was increased with the passage of lime and at the same time,the comparison of average daily feed intake of mice in each group was not significantly different(P>0.05).Conclusions:G.uralensis Fisch polysaccharide can significantly improve the growth performance and immunologic function of mice and laid a research basis for the clinical application of G.uralensis Fisch polysaccharide. | Jie Chen Xiao-Qing Zhu Li Yang Yan Luo Meng-Yuan Wang Xiao-Ting Liu Ke-Xun Liang Xin-Li Gu | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，11： | 22 |
| 6 | Transplantation of autologous bone marrow mononuclear cells for patients with lower limb ischemia显示文摘背景为更低的手足局部缺血的许多治疗选择是困难的与差的动脉的流出或与医疗的一般 conditions.The 效果独自远离的穷人一起申请病人 ideal.especially 在学习试图探索移植的效果的这些 patients.This 在有糖尿病的 foot.A 高水平切断的病人是不可避免的自体同源更低的手足局部缺血并且到的治疗上的骨头髓 mononuclear 房间比较 intra-artedal | GU Yong-quan ZHANG Jian GUO Lian-rui QI Li-xing ZHANG Shu-wen XU Juan LI Jian-xin LUO Tao JI Bing-xin LI Xue-feng YU Heng-xi CUI Shi-jun WANG Zhong-gao | 2008 | Chinese Medical Journal2008，，11： | 21 |
| 7 | Clinical trial with traditional Chinese medicine intervention ''tonifying the kidney to promote liver regeneration and repair by affecting stem cells and their microenvironment'' for chronic hepatitis B-associated liver failure显示文摘AIM:To study the clinical efficacy of traditional Chinese medicine(TCM)intervention'tonifying the kidney to promote liver regeneration and repair by affecting stem cells and their microenvironment'('TTK')for treating liver failure due to chronic hepatitis B.METHODS:We designed the study as a randomized controlled clinical trial.Registration number of Chinese Clinical Trial Registry is Chi CTR-TRC-12002961.A total of 144 patients with liver failure due to infection with chronic hepatitis B virus were enrolled in this randomized controlled clinical study.Participants were randomly assigned to the following three groups:(1)a modern medicine control group(MMC group,36patients);(2)a'tonifying qi and detoxification'('TQD')group(72 patients);and(3)a'tonifying the kidney to promote liver regeneration and repair by affecting stem cells and their microenvironment'('TTK')group(36patients).Patients in the MMC group received general internal medicine treatment;patients in the'TQD'group were given a TCM formula'tonifying qi and detoxification'and general internal medicine treatment;patients in the'TTK'group were given a TCM formula of'TTK'and general internal medicine treatment.All participants were treated for 8 wk and then followed at 48 wk following their final treatment.The primaryefficacy end point was the patient fatality rate in each group.Measurements of various virological and biochemical indicators served as secondary endpoints.The one-way analysis of variance and the t-test were used to compare patient outcomes in the different treatment groups.RESULTS:At the 48-wk post-treatment time point,the patient fatality rates in the MMC,'TQD',and'TTK'groups were 51.61%,35.38%,and 16.67%,respectively,and the differences between groups were statistically significant(P<0.05).However,there were no significant differences in the levels of hepatitis B virus DNA or prothrombin activity among the three groups(P>0.05).Patients in the'TTK'group had significantly higher levels of serum total bilirubin compared to MMC subjects(339.40μmol/L±270.09μmol/L vs 176.13μmol/L±185.70μmol/L,P=0.014).Serum albumin levels were significantly increased in both the'TQD'group and'TTK'group as compared with the MMC group(31.30 g/L±4.77g/L,30.72 g/L±2.89 g/L vs 28.57 g/L±4.56 g/L,P<0.05).There were no significant differences in levels of alanine transaminase among the three groups(P>0.05).Safety data showed that there was one case of stomachache in the'TQD'group and one case of gastrointestinal side effect in the'TTK'group.CONCLUSION:Treatment with'TTK'improved the survival rates of patients with liver failure due to chronic hepatitis B.Additionally,liver tissue was regenerated and liver function was restored. | Han-Min Li Zhi-Hua Ye Jun Zhang Xiang Gao Yan-Ming Chen Xin Yao Jian-Xun Gu Lei Zhan Yang Ji Jian-Liang Xu Ying-He Zeng Fan Yang Lin Xiao Guo-Guang Sheng Wei Xin Qi Long Qing-Jing Zhu Zhao-Hong Shi Lian-Guo Ruan Jia-Yao Yang Chang-Chun Li Hong-Bin Wu Sheng-Duo Chen Xin-La Luo | 2014 | World Journal of Gastroenterology2014，20，48： | 20 |
| 8 | New precut sphincterotomy for endoscopic retrograde cholangiopancreatography in difficult biliary duct cannulation显示文摘AIM:To retrospectively investigate the effect and safety of various new type precut sphincterotomy techniques (VNTPST) in endoscopic retrograde cholangiopancreato-graphy (ERCP) due to difficult biliary duct cannulation (DBC). METHODS:A plough-like pull-type sphincterotome (PLPTS) or improved short nose sphincterotome or improved needle knife was applied. VNTPST was carried out in 30 of 280 patients,whose biliary tract could not be exposed well or deep cannulation was difficult to perform during ERCP with traditional methods. Patients were followed up for short-term complications and the therapeutic effect of VNTPS was observed and compared with that of traditional endoscopic sphincterotomy (EST). RESULTS:A total 280 patients underwent ERCP,of which 3 failed in operation because of pathological features in stomch or duodenum,247 successfully underwent traditional ERCP (89.1%,247/277),30 failed (10.8%,30/277). VNTPS technique succeeded in 24 (80%,24/30) of 30 cases. The successful rate of deep biliary duct cannulation increased 8.6% (24/277),the total cannulation successful rate following precut was 97.7%. There was a significant difference between the two groups (97.7% vs 89.1%,χ2 = 17.1,P < 0.01). The incidence of complications was 9.3% (26/277) for traditional ERCP group and 13.3% (4/30) for VNTPS technique group. Guideline tip was broken in pancreatic duct (KPDGP) of one patient,and there was no pancreatitis,slight or moderate bleeding postoperatively occurred in 2 patients,1 patient had bleeding during operation (PDWN). There were no differences between VNTPS technique group and traditional ERCP (TRERCP)group (13.3% vs 9.3%,χ2 = 0.478,P > 0.05). CONCLUSION:VNTPS procedure and Deng's precut are highly effective methods to get biliary access during ERCP with DBC. With skillful techniques,it can increase the successful rate for deep cannulation of biliary duct and decrease complications. VNTPS technique,especially Deng's precut is as effective and safe as EST. This technique can be well performed in hospitals without particular equipments. | Deng-Hao Deng Hong-Nei Zuo Jia-Feng Wang Zhi-E Gu Hong Chen Yuan Luo Ming Chen Wen-Nuo Huang Lu Wang Wei Lu | 2007 | World Journal of Gastroenterology2007，13，32： | 20 |
| 9 | Baicalin attenuates high fat diet-induced insulin resistance and ectopic fat storage in skeletal muscle,through modulating the protein kinase B/Glycogen synthase kinase 3 beta pathway显示文摘Insulin resistance is the pathophysiological basis of many diseases.Overcoming early insulin resistance highly significant in prevention diabetes,non-alcoholic fatty liver,and atherosclerosis.The present study aimed at evaluating the therapeutic effects of baicalin on insulin resistance and skeletal muscle ectopic fat storage in high fat diet-induced mice,and exploring the potential molecular mechanisms.Insulin resistance in mice was induced with a high fat diet for 16 weeks.Animals were then treated with three different doses of baicalin(100,200,and 400 mg·kg^(-1)·d^(-1)for 14 weeks.Fasting blood glucose,fasting serum insulin,glucose tolerance test(GTT),insulin tolerance test(ITT),and skeletal muscle lipid deposition were measured.Additionally,the AMP-activated protein kinase/acetyl-CoA carboxylase and protein kinase B/Glycogen synthase kinase 3 beta pathways in skeletal muscle were further evaluated.Baicalin significantly reduced the levels of fasting blood glucose and fasting serum insulin and attenuated high fat diet induced glucose tolerance and insulin tolerance.Moreover,insulin resistance was significantly reversed.Pathological analysis revealed baicalin dose-dependently decreased the degree of the ectopic fat storage in skeletal muscle.The properties of baicalin were mediated,at least in part,by inhibition of the AMPK/ACC pathway,a key regulator of de novo lipogenesis and activation of the Akt/GSK-3β pathway,a key regulator of Glycogen synthesis.These data suggest that baicalin,at dose up to 400 mg·kg^(-1)·d^(-1),is safe and able to attenuate insulin resistance and skeletal muscle ectopic fat storage,through modulating the skeletal muscle AMPK/ACC pathway and Akt/GSK-3β pathway. | XI You-Li LI Hong-Xia CHEN Chen LIU Ya-Qun LV Hong-Mei DONG Shi-Qi LUO Er-Fei GU Ming-Bo LIU Hua | 2016 | Chinese Journal of Natural Medicines2016，14，1： | 19 |
| 10 | Traditional Chinese medicine integrated with chemotherapy for stage Ⅳ non-surgical gastric cancer: a retrospective clinical analysis显示文摘OBJECTIVE: Traditional Chinese medicine(TCM) is regarded as an important treatment for gastric cancer patients, especially for those in advanced stage. To evaluate the effects of TCM treatment on gastric cancer patients, the authors performed a retrospective study to report the result of the integrated treatment of TCM with chemotherapy for stage IV non-surgical gastric cancer.METHODS: In this study, 182 patients with stage IV and non-surgical gastric cancer were retrospectively analyzed to evaluate the effects of TCM integrated with chemotherapy. Among the 182 cases, 88 cases received integrated therapy consisting of TCM and chemotherapy, while 94 cases received chemotherapy alone. The overall survival and Karnofsky performance status(KPS) score were measured as the main outcome.RESULTS: The median overall survival of the integrated therapy group and chemotherapy group were 16.9 and 10.5 months, respectively. The 1-, 3-and 5-year survival rates of integrated therapy group vs. chemotherapy group were 70% vs. 32%, 18% vs. 4%, and 11% vs. 0%, respectively. There was a significant difference between the two groups(χ2 = 42.244, P < 0.001). After six-month treatment, KPS scores of the integrated therapy group and the chemotherapy group were 75.00 ± 14.78 and 60.64 ± 21.39, respectively(P < 0.001). The Cox regression analysis showed that TCM treatment is a protective factor for patients' overall survival. CONCLUSION: This study demonstrated that TCM integrated with chemotherapy may prolong overall survival and improve survival rate and life quality of patients with stage IV non-surgical gastric cancer. | xuan liu li-juan xiu jian-peng jiao jing zhao ying zhao ye lu jun shi yong-jin li min ye yu-fang gu xiao-wei wang jing-yu xu ci-an zhang yong-ying liu yun luo xiao-qiang yue | 2017 | Journal of Integrative Medicine2017，15，6： | 18 |
| 11 | Surgical treatment of 82 patients with diabetic lower limb ischemia by distal arterial bypass显示文摘背景糖尿病的更低的手足局部缺血是糖尿病 mellitus.This 学习的严肃的复杂并发症被进行调查有效性远侧动脉与更低的手足局部缺血在糖尿病的病人绕过治疗。从 2000 年 7 月的方法到 2004 年 7 月,有严重更低的手足局部缺血的 82 个糖尿病的病人(类型 2 ) 的 96 更低的手足在吴璇被对待医院。动脉与 femoro 腿弯部的 polytetrafluoroethylene (PTFE ) 和接枝胫骨的自体同源的接枝绕过在 311 手足(32.3%) 上被执行。腿弯部胫骨的动脉独自绕过在 22 手足(22.9%) 上被执行。联合肠骨的动脉 stenting, femoro 腿弯部的动脉 PTFE 接枝绕过,并且接枝胫骨的动脉自体同源的接枝绕过在 12 上被执行手足(12.5%) ,和 femoro 胫骨的动脉接枝绕过在 10 手足(10.4%) 上被执行。Popliteal-tibial-pedal 动脉接枝绕过在 7 手足(7.3%) 上被执行。结果在 79 个病人的 92 手足的动脉的接枝是分泌物上的专利。有 4 ischemic 手足(3.7%) 的三个病人死于呼吸失败在操作以后的 12 个小时, 3 天和 7 天分别地。早操作成功率是 96.3%(79/82 ) 。分泌物上的病人的接枝明显率是 95.8%(92/96 ) 。短期的全部的有效性率是 83.3%(80/96 ) 。脚溃疡愈合率是 35.7%(10/28 ) 。97.4%(75/77 ) 病人们被跟随在上面为 13.5 个月的一个平均数。长期的全部的有效的率是 80.7%(71/88 ) 。全部的切断率是 4.5%(4/88 ) 。死亡是 4.5% 。全部的接枝明显率是 90.9%(80/88 ) 在糖尿病的脚的治疗的 .Conclusion,远侧的更低的手足动脉绕过罐头帮助避免切断或降低切断水平,并且可以支持脚溃疡愈合并且改进病人生活的质量。 | GU Yong-quan ZHANG Jian QI Li-xing YU Heng-xi LI Jian-xin LI Xue-feng GUO Lian-rui LUO Tao CUI Shi-jun WANG Zhong-gao | 2007 | Chinese Medical Journal2007，，2： | 16 |
| 12 | Prevalence, awareness, treatment, and control of hypertension in the non-dialysis chronic kidney disease patients显示文摘 | ZHENG Ying CAI Guang-yan CHEN Xiang-mei FU Ping CHEN Jiang-hua DING Xiao-qiang YU Xue-qing LIN Hong-li LIU Jian XIE Ru-juan WANG Li-ning NI Zhao-hui LIU Fu-you YIN Ai-ping XING Chang-ying WANG Li SHI Wei LIU Jian-she HE Ya-ni DING Guo-hua LI Wen-ge WU Guang-li MIAO Li-ning CHEN Nan SU Zhen MEI Chang-lin ZHAO Jiu-yang GU Yong BAI Yun-kai LUO Hui-min LIN Shan CHEN Meng-hua GONG Li YANG Yi-bin YANG Xiao-ping LI Ying WAN Jian-xin WANG Nian-song LI Hai-ying XI Chun-sheng HAO Li XU Yan FANG Jing-ai LIU Bi-cheng LI Rong-shan WANG Rong ZHANG Jing-hong WANG Jian-qin LOU Tan-qi SHAO Feng-min MEI Feng LIU Zhi-hong YUAN Wei-jie SUN Shi-ren ZHANG Ling ZHOU Chun-hua CHEN Qin-kai JIA Shun-lian GONG Zhi-feng GUAN Guang-ju XIA Tian ZHONG Liang-bao | 2013 | Chinese Medical Journal2013，，12： | 16 |
| 13 | Polyphyllin I inhibits proliferation and metastasis of ovarian cancer cell line HO-8910PM invitro显示文摘OBJECTIVE: To study the anticancer mechanism of polyphyllin I (PPI), a Traditional Chinese Medicine, on the ovarian cancer cell line HO-8910PM invitro. METHODS: Transwell chamber invasive assays were used to investigate the inhibitory capacity of PPI on HO-8910PM metastasis. Gene expression profiling chips was used to screen differentially expressed genes between experiment group and control group. Reverse transcription PCR and Western blotting were used to determine mRNA and protein levels. RESULTS: With increasing PPI concentration, the metastatic capacity of cells decreased, with significance differences between the experimental and control groups (P<0.01) as well as between two concentration groups. Gene expression profiling identified 123 differentially expressed genes, of which 70 were downregulated and 53 were upregulated. The genes were involved in multiple signal transduction pathways, including apoptosis, proliferation and metastasis. Real-time PCR (RT-PCR) showed that differential genes PIK3C2B, Caspase 9and Wnt5A were downregulated with increasing PPI, showing an evident dose-effect relationship. The c-Jun was an exception. As the PPI dosage increased and the exposure time was extended, c-Jun relative expression showed an upward trend. There were significant differences between the experiment and control (P<0.05). Western blot analyses showed that PPI treatment decreased levels of Caspase 9, Wnt5A and PIK3C2B and increased activatedCaspase9,c-Junandp-c-Junexpressionlevels. CONCLUSION: PPI has strong antitumor and anti transfer activity. It can activate c-Jun expression and the JNK signaling pathway, elicit cell apoptosis via the mitochondrial-mediated Caspase activation pathway, and finally inhibit tumor growth and mi- gration in vitro. The downregulation of PIK3C2B and Wnt5A jointly inhibit the proliferation and metastasis of HO-8910PM. PPI may be a novel treatment for ovarian cancer. | Linhui Gu Jianguo Feng Haiyan Xu Min Luo Dan Su | 2013 | Journal of Traditional Chinese Medicine2013，33，3： | 15 |
| 14 | Electroacupuncture activates enteric glial cells and protects the gut barrier in hemorrhaged rats显示文摘AIM:To investigate whether electroacupuncture ST36 activates enteric glial cells,and alleviates gut inflammation and barrier dysfunction following hemorrhagic shock.METHODS:Sprague-Dawley rats were subjected to approximately 45% total blood loss and randomly divided into seven groups:(1) sham:cannulation,but no hemorrhage;(2) subjected to hemorrhagic shock(HS);(3) electroacupuncture(EA) ST36 after hemorrhage;(4) vagotomy(VGX)/EA:VGX before hemorrhage,then EA ST36;(5) VGX:VGX before hemorrhage;(6) a-bungarotoxin(BGT)/EA:intraperitoneal injection of a-BGT before hemorrhage,then EA ST36; and(7) a-BGT group:a-BGT injection before hemorrhage.Morphological changes in enteric glial cells(EGCs) were observed by immunofluorescence,and glial fibrillary acidic protein(GFAP; a protein marker of enteric glial activation) was evaluated using reverse transcriptase polymerase chain reaction and western blot analysis.Intestinal cytokine levels,gut permeability to 4-k Da fluorescein isothiocyanate(FITC)-dextran,and the expression and distribution of tight junction protein zona occludens(ZO)-1 were also determined.RESULTS:EGCs were distorted following hemorrhage and showed morphological abnormalities.EA ST36 attenuated the morphological changes in EGCs at 6 h,as compared with the VGX,a-BGT and HS groups.EA ST36 increased GFAP expression to a greater degree than in the other groups.EA ST36 decreased intestinal permeability to FITC-dextran(760.5 ± 96.43 ng/m L vs 2466.7 ± 131.60 ng/m L,P < 0.05) and preserved ZO-1 protein expression and localization at 6 h afterhemorrhage compared with the HS group.However,abdominal VGX and a-BGT treatment weakened or eliminated the effects of EA ST36.EA ST36 reduced tumor necrosis factor-a levels in intestinal homogenates after blood loss,while vagotomy or intraperitoneal injection of a-BGT before EA ST36 abolished its antiinflammatory effects.CONCLUSION:EA ST36 attenuates hemorrhageinduced intestinal inflammatory insult,and protects the intestinal barrier integrity,partly via activation of EGCs. | Sen Hu Zeng-Kai Zhao Rui Liu Hai-Bin Wang Chun-Yu Gu Hong-Min Luo Huan Wang Ming-Hua Du Yi Lv Xian Shi | 2015 | World Journal of Gastroenterology2015，21，5： | 15 |
| 15 | Complications of high intensity focused ultrasound in patients with recurrent and metastatic abdominal tumors显示文摘AIM: To analyze the local and systemic complications of high intensity focused ultrasound (HIFU) for patients with recurrent and metastatic abdominal tumors.METHODS: From Aug 2001 to Aug 2004, 17 patients with recurrent and metastatic abdominal tumors were enrolled in this study. Real-time sonography was taken, and vital signs, liver and kidney function, skin burns, local reactions, and systemic effects were observed and recored before, during, and after HIFU. CT and MRI were also taken before and after HIFU.RESULTS: All 17 patients had skin burns and pain in the treatment region; the next common complication was neurapraxia of the stomach and intestines to variable degrees. The other local and systemic complications were relatively rare. Severe complications were present in two patients; one developed a superior mesenteric artery infarction resulting in necrosis of the entire small intestines, and the other one suffered from a perforation in terminal ileum due to HIFU treatment. CONCLUSION: Although HIFU is a one of noninvasive treatments for the recurrent and metastatic abdominal tumors, there are still some common and severe complications which need serious consideration. | Jian-Jun Li Guo-Liang Xu Mo-Fa Gu Guang-Yu Luo Zhang Rong Pei-Hong Wu Jian-Chuan Xia | 2007 | World Journal of Gastroenterology2007，13，19： | 14 |
| 16 | Total pollution effect of urban surface runoff显示文摘For pollution research with regard to urban surface runoff,most sampling strategies to date have focused on differences in land usage. With single land-use sampling,total surface runoffpollution effect cannot be evaluated unless every land usage spot is monitored. Through a new sampling strategy known as mixed stormwater sampling for a street community at discharge outlet adjacent to river,this study assessed the total urban surface runoffpollution effect caused by a variety of land uses and the pollutants washed off from the rain pipe system in the Futian River watershed in Shenzhen City of China.The water quality monitoring indices were COD(chemical oxygen demand),TSS(total suspend solid),TP(total phosphorus),TN(total nitrogen)and BOD(biochemical oxygen demand).The sums of total pollution loads discharged into the river for the four indices of COD,TSS,TN,and TP over all seven rainfall events were very different.The mathematical model for simulating total pollution loads was established from discharge outlet mixed stormwater sampling of total pollution loads on the basis of four parameters:rainfall intensity,total land area,impervious land area,and pervious land area.In order to treat surface runoffpollution,the values of MFF30(mass first flush ratio)and FF30(first 30%of runoffvolume) can be considered as split-flow control criteria to obtain more effective and economical design of structural BMPs(best management practices)facilities. | LUO Hongbing LUO Lin HUANG Gu LIU Ping LI Jingxian HU Sheng WANG Fuxiang XU Rui HUANG Xiaoxue | 2009 | Journal of Environmental Sciences2009，21，9： | 13 |
| 17 | A novel cognitive ISAR imaging method with random stepped frequency chirp signal显示文摘The random stepped frequency chirp signal(RSFCS)has better performance in anti-jamming than that of conventional stepped frequency chirp signal(SFCS).In combination with the theory of compressing sensing(CS),a novel ISAR imaging method is proposed based on RSFCS,in which the high resolution range profile(HRRP)is reconstructed by using the conventional OMP algorithm,whereas the cognitive approach is introduced to further reduce the number of sub-pulse in RSFCS.In the proposed method,via cognizing the characteristics of moving targets,the number of sub-pulse in each burst can be adjusted adaptively.Finally,in the cross-range direction,the accurate reconstruction of ISAR image by using CS theory is implemented,which can effectively accomplish unwrapping.With the proposed method,high quality HRRP and ISAR image can be achieved with fewer sub-pulses of RSFCS and lower burst repetition frequency(BRF).Some simulation results are given to validate the effectiveness and robustness of the proposed algorithm. | ZHU Feng ZHANG Qun LUO Ying LI KaiMing GU FuFei | 2012 | Science China(Information Sciences)2012，55，8： | 13 |
| 18 | Parthenogenetic haploid embryonic stem cells produce fertile mice显示文摘 | Haifeng Wan Zhengquan He Mingzhu Dong Tiantian Gu Guan-Zheng Luo Fei Teng Baolong Xia Wei Li Chunjing Feng Xin Li Tianda Li Ling Shua Rui Fu Liu Wang Xiu-Jie Wang Xiao-Yang Zhao Qi Zhou | 2013 | Cell Research2013，23，11： | 13 |
| 19 | Baicalin extracted from Huangqin(Radix Scutellariae Baicalensis) induces apoptosis in gastric cancer cells by regulating B cell lymphoma(Bcl-2)/Bcl-2-associated X protein and activating caspase-3 and caspase-9显示文摘OBJECTIVE:To evaluate the effects of baicalin in human gastric cancer cells, including apoptosis-inducing effects, and to investigate its underlying mechanisms of action.METHODS:Cell proliferation and apoptosis assays were performed to investigate the anti-proliferation effects of baicalin in human gastric cancer BGC-823 and MGC-803 cells.Real time-quantitative polymerase chain reaction and Western blotting analysis were performed to elucidate the molecular mechanisms underlying the anti-tumor properties of baicalin.RESULTS:In BGC-823 and MGC-803 gastric cancer cells treated with 80, 120, and 160 μmol/L baicalin for 48 h, a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay showed that baicalin significantly inhibited cell proliferation in a dose-dependent manner, while flow cytometric analysis demonstrated that baicalin could induce apoptosis, also in a dose-dependent manner.Moreover, baicalin up-regulated the expression of caspase-3, caspase-9, and B cell lymphoma(Bcl-2)-associated X protein and down-regulated the expression of Bcl-2 at both the m RNA and protein level.CONCLUSION:Baicalin has potential as a therapeutic agent for gastric cancer by inducing apoptosis in cancer cells. | Wang Hongwei Li Hailong Chen Fengqin Luo Jun Gu Jing Wang Huping Wu Hongyan Xu Yan | 2017 | Journal of Traditional Chinese Medicine2017，37，2： | 13 |
| 20 | Low-temperature processed inorganic hole transport layer for efficient and stable mixed Pb-Sn low-bandgap perovskite solar cells显示文摘Metal halide perovskite solar cells(PSCs)have attracted tremendous attention as an emerging photovoltaic technology due to their high efficiency,low cost and ease of fabrication from earth-abundant materials[1,2].The power conversion efficiencies(PCEs)have been rapidly boosted from 3.8%in the pioneer’s work to a certified 24.2%nowadays in just ten years[3].The PCE breakthroughs in PSCs have mostly adopted full lead-based perovskites(APbX3)with bandgaps of 1.5–1.6 eV. | Qiaolei Han Ying Wei Renxing Lin Zhimin Fang Ke Xiao Xin Luo Shuai Gu Jia Zhu Liming Ding Hairen Tan | 2019 | Science Bulletin2019，64，19： | 13 |
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