|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | Influence of the sea rush Juncus martitimus on metal concentration and speciation in estuarine sediment colonized by the plant显示文摘 | Marisa C RAlmeida Ana P Mucha Teresa M S D Vasconce- los | 2004 | Environmental Science and Technology2004，38，： | 1 |
| 3 | Dose the winemaking process influence the wine 87Sr/86Sr显示文摘 | Marisa C Almeida R | 2004 | Food Chemistry2004，8，5： | 1 |
| 4 | Influence of the sea rush Juncus martitimus on metal concentration and speciation in estuarin~ sediment colonized by the plant 显示文摘 | Marisa C Almeida R Ana P Mucha | 2004 | Environmental Science and Technology2004，38，： | 1 |
| 5 | Comparison of the role of the sea club-rush scirpus maritimus and the sea rish Juncus maritimus in terms of concentration,speciation and bioaccumulation of metals in the estuarine sediment显示文摘 | ALMEIDA C MARISA R MUCHA A P | | 0，，11： | 1 |
| 6 | Synthesis,structural characterization and biological activity of p-fluorobenzaldehyde thiosemicarbazones and of a nickel complex显示文摘 | MARISA B F SILVIA C GIOIA R | 2000 | J Inorg Biochem2000，81，： | 1 |
| 7 | Determination of ammonia based on the electro-oxidation of hydroquinone in dimethylformamide or in the room temperature ionic liquid, 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide显示文摘 | Debora Giovanelli Marisa C Buzzeo Nathan S Lawrence Christopher Hardacre Kenneth R Seddon Richard G Compton | 2003 | Talanta2003，，： | 1 |
| 8 | Short-term changes in nitrogen availability, gas fluxes (CO2, NO, N2O) and microbial biomass after tillage during pasture re-establishment in Rondonia, Brazil 显示文摘 | JANAINA B D C MARISA D C P CRISTIANO A D A | 2007 | Soil & Tillage Research2007，96，： | 1 |
| 9 | Synthesis and characterization of squarare planar nickel (Ⅱ) complexes with p-fluorobenzaldehyde thiosemicarbazones derivativesd显示文摘 | MARISA B F SILIVIA C FRANCO B | 2001 | J Inorg Chim Acta2001，312，： | 1 |
| 10 | Influence of surfactants on the Cu phytoremediation potential of a salt marsh plant 显示文摘 | C Marisa R Mmeida A Claudia Dias Aria P Mucha | 2009 | Chemosphere2009，75，： | 1 |
| 11 | ^sup 18^F-FDG PET After 2 Cycles of ABVD Predicts Event-Free Survival in Early and Advanced Hodgkin Lymphoma显示文摘 | Cerci Juliano J Pracchia Luís F Linardi Camila C G Pitella Felipe A Delbeke Dominique Izaki Marisa Trindade Evelinda Junior José Soares Buccheri Valeria Meneghetti José C | 2010 | The Journal of Nuclear Medicine2010，，： | 1 |
| 12 | Does the winemaking process influence the wine 87Sr/86Sr?A case study显示文摘 | Marisa C Almeida R Teresa M | 2004 | Food Chemistry2004，85，： | 1 |
| 13 | A ladder of community participation for underdevelopment countries 显示文摘 | Marisa B Guaraldo C | 1996 | habitat intl1996，20，3： | 1 |
| 14 | Visual attention :the past 25 years显示文摘 | MARISA C | 2011 | Visio Research2011，51，13： | 1 |
| 15 | Mutations of the Light Meromyosin Domain of the β-Myosin Heavy Chain Rod in Hypertrophic Cardiomyopathy显示文摘 | EDWARD BLAIR CHARLES REDWOOD MARISA DE JESUS OLIVEIRA J C | 2002 | Circulation Research2002，90，： | 1 |
| 16 | The effect of addition of different elastomers upon the crystalline nature of PP 显示文摘 | Silva A L N Marisa C G Lopes R | 2001 | J Appl Polym Sci2001，81，14： | 1 |
| 17 | Optimiza- tion of levofloxacin-loaded crosslinked chitosan microspheres for in- haled aerosol therapy显示文摘 | Marisa C Gaspar Jo Alberto A C C Pais | 2015 | Eur J Pharm Biopharm2015，96，： | 1 |
| 18 | Denaturing gradient gel electripboresis analysis of the 16S rDNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausage显示文摘 | Luca C Marisa M Carlo C | | 0，，11： | 1 |
| 19 | Binuclear copper(I) complexes containing bis(diphenylphosphino)methane bridging ligands:crystal structure of 2 显示文摘 | Josefina Díez Gamasa M Pilar José Gimeno Antonio T Marisa T C | 1987 | J Chem Soc Dalton Trans1987，，： | 1 |
| 20 | Sphingolipids and cancer:ceramide and sphingosine - I - phosphate in the regulation of cell death and drug resistance 显示文摘 | Ponnusamy S Marisa M Senkal C | 2010 | Future Oncol2010，6，10： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员