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| 1 | An Efficient Procedure for Isolating Microsatellite DNAs from Sea Cucumber(Apostichopus japonicus)显示文摘The construction of enrichment library proves to be one of the efficient approaches for isolating microsatellites in this study. The genomic DNA of sea cucumber was digested with HaeIII and size-selected DNA fragments (250-700 bp) were ligated to an adaptor. Microsatellite-containing sequences were captured by using a combination of GA and CA probes, which were attached to a nylon membrane. The microsatellite enrichment library constructed in this study consisted of approximately 700 clones. Two hun-dred and thirty-two clones reacted positively after the library screening procedure. Of the 50 clones sequenced, all contained at least one microsatellite and one duplicate clone was found. Approximately 86% of the sequenced fragments permitted to design primers for sequence tagged microsatellite site (STMS). | HU Jingjie, ZHAN Aibin, LU Wei, HU Xiaoli, and BAO Zhenmin* Laboratory of Marine Genetics and Breeding (MGB), Division of Life Science and Biotechnology, Ocean University of China, Qingdao 266003, P. R. China | 2007 | Journal of Ocean University of China2007，6，1： | 2 |
| 2 | Management of dislocated hips with pavlik harness treatment and ultrasound monitoring显示文摘 | Harding MGB Hons BSc Harcke HT | 1997 | J Pediatr Orthop1997，17，： | 1 |
| 3 | Healthcare- associated staphylococcus aureus bloodstream infection: length of stay, attributable mortality, and additional direct costs显示文摘 | Primoa MGB Guilarde AO Martelli CMT | 2012 | Braz J Infects Dis2012，16，6： | 1 |
| 4 | Contrasting properties of hypoxia-inducible factor 1 (HIF-1) and HIF-2 in yon Hippel-Lindau-associated renal cell carcinoma显示文摘 | Raval RR Lau KW Tran MGB | 2005 | Mol Cell Biol2005，25，13： | 1 |
| 5 | Asic Clinical and Laboratory Features of Filoviral Hemorrhagic Fever 显示文摘 | Kortepeter MGB Bausch DG Bray M | 2011 | J Infect Dis2011，204，3： | 1 |
| 6 | Chemical variability of the volatiles of Copaifera langsdorffii growing wild in the Southeastern part of Brazil显示文摘 | do Nascimento ME Zoghbi MGB Pinto JEBP | 2012 | Biochemical Systematics and Ecology2012，43，： | 1 |
| 7 | CA125, a vavid marker in ovarian carcinoma patients treated with paclitaxal显示文摘 | Johannes MGB Rob AV | 1996 | Cancer1996，78，： | 1 |
| 8 | Management of dislocated hips with pavlik harness treatment and ultrasound monitoring显示文摘 | Harding MGB Hons BSc Harcke HT | 1997 | J Pediatr Orthop1997，17，： | 1 |
| 9 | Divergent and comvergent forms of a new schiff-base cryptanel: X-ray crystallographic and molecular mechanics investigation显示文摘 | Drew MGB Hunter J Marrs D | 1992 | J Chem Soc Dalton Trans1992，11，： | 1 |
| 10 | Cascade complexes of an octaaza cryptand: co-ordinated azide with linear M-NNN-M geometry显示文摘 | Drew MGB Hunter J Marrs D | 1992 | J Chem Soc Dalton Trans1992，3235，： | 1 |
| 11 | Identification and stability of the artemisinin pharmacophore:studies of the reduetive decomposition of deoxyartemisinins and deoxyarteethers and the implications for the mode of antimalaria action显示文摘 | DREW MGB METCALFE J DASCOMBE MJ | 2007 | J Mol Struct Theochem2007，82，： | 1 |
| 12 | SPATULA, a gene that controls development of carpel margin tissues in Ara- bidopsis, encodes a bHLH protein 显示文摘 | Heisler MGB Atkinson A Bylstra YH | 2001 | Development2001，128，： | 1 |
| 13 | Laser induced Coulomb explosion of gold nanoparticles:application of nanophotolysis for Breast cancer显示文摘 | Ashiq MGB Saeed MA Noorddin Ibrahim | | 0，，： | 1 |
| 14 | Contrasting properties ofhypoxia-inducible factor 1 ( hif-1 ) and hif-2 in von hippel-lindau-associated renal cell carcinoma 显示文摘 | Raval RR Lau KW Tran MGB | 2005 | Mol Cell Biol2005，25，13： | 1 |
| 15 | A anew ditopic polyaza macrobicyclic ligand: X-ray crystallographic structure determination显示文摘 | Drew MGB Mcdowell D Nelson J | 1988 | Polyhedron1988，7，： | 1 |
| 16 | Novel nanophotolysis technique for breast cancer therapy显示文摘 | Ashiq MGB Ibrahim N Shahid M | | 0，，： | 1 |
| 17 | Numerical study of nanophotolysis approach for breast cancer显示文摘 | Ashiq MGB Saeed NA Ibrahim N | | 0，，： | 1 |
| 18 | 6, 6'-bis-(5, 6 diethyl-triazin-3- yl)-2, 2'-bipyridyl the first example of a new class of quadridentate heterocyclie extraction rea gents for the separation of americium ( Ⅲ ) and europium(Ⅲ) 显示文摘 | DREW MGB FOREMAN MR S J HILL C | 2005 | Inorg Chem Commun2005，8，3： | 1 |
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