| 3 | Organic transistor platform with integrated microfluidics for in-line multi-parametric in vitro cell monitoring显示文摘Future drug discovery and toxicology testing could benefit significantly from more predictive and multi-parametric readouts from in vitro models.Despite the recent advances in the field of microfluidics,and more recently organ-on-a-chip technology,there is still a high demand for real-time monitoring systems that can be readily embedded with microfluidics.In addition,multi-parametric monitoring is essential to improve the predictive quality of the data used to inform clinical studies that follow.Here we present a microfluidic platform integrated with in-line electronic sensors based on the organic electrochemical transistor.Our goals are twofold,first to generate a platform to host cells in a more physiologically relevant environment(using physiologically relevant fluid shear stress(FSS))and second to show efficient integration of multiple different methods for assessing cell morphology,differentiation,and integrity.These include optical imaging,impedance monitoring,metabolite sensing,and a wound-healing assay.We illustrate the versatility of this multi-parametric monitoring in giving us increased confidence to validate the improved differentiation of cells toward a physiological profile under FSS,thus yielding more accurate data when used to assess the effect of drugs or toxins.Overall,this platform will enable high-content screening for in vitro drug discovery and toxicology testing and bridges the existing gap in the integration of in-line sensors in microfluidic devices. | Vincenzo F.Curto Bastien Marchiori Adel Hama Anna-Maria Pappa Magali P.Ferro Marcel Braendlein Jonathan Rivnay Michel Fiocchi George G.Malliaras Marc Ramuz Róisín M.Owens | 2017 | Microsystems & Nanoengineering2017，3，1： | 0 |
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