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| 1 | Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells显示文摘BACKGROUND Human adipose-derived stromal/stem cells(hASCs)are one of the most useful types of mesenchymal stromal/stem cells,which are adult multipotent cells with great therapeutic potential for the treatment of several diseases.However,for successful clinical application,it is critical that high-quality cells can be obtained.Diverse factors seem to be able to influence cell quality and performance,especially factors related to donors’intrinsic characteristics,such as age.Nevertheless,there is no consensus regarding this characteristic,and there is conflicting information in the literature.AIM To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young donors.METHODS hASCs were harvested from liposuctioned adipose tissue obtained from female donors(aged 20-70 years).Cells were distributed into two groups according to age range:old hASCs(oASCs,≥55 years,n=9)and young hASCs(yASCs,≤35 years,n=9).For each group,immunophenotypic characterization was performed by flow cytometry.Population doubling time was assessed over seven days.For adipogenic potential evaluation,lipid deposits were assessed after 7 d,14 d and 21 d of adipogenic induction.Osteogenic potential was verified by analyzing cell mineralization after 14 d,21 d and 28 d of osteogenic induction.mRNA expression of PPARγ2,CEBPA and Runx2 were detected by quantitative reverse transcription polymerase chain reaction.RESULTS hASCs were successfully obtained,cultured,and grouped according to their age:yASCs(26.33±4.66 years old)and oASCs(64.78±4.58 years old).After maintenance of the cells in culture,there were no differences in morphology between cells from the young and old donors.Additionally,both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal cells.The average doubling time indicated that yASCs(4.09±0.94 d)did not significantly differ from oASCs(4.19±1.29 d).Concerning differentiation potential,after adipogenic and osteogenic induction,yASCs and oASCs were able to differentiate to greater levels than the noninduced control cells.However,no differences were found in the differentiation efficiency of yASCs and oASCs in adipogenesis or osteogenesis.Additionally,the mRNA expression of PPARγ2,CEBPA and Runx2 were similar in yASCs and oASCs.CONCLUSION Our findings suggest that age does not seem to significantly affect the cell division or adipogenic or osteogenic differentiation ability of adipose-derived stem cells isolated from lipoaspirates. | Cintia DS Horinouchi María Julia Barisón Anny W Robert Crisciele Kuligovski Alessandra M Aguiar BrunoDallagiovanna | 2020 | World Journal of Stem Cells2020，12，12： | 2 |
| 2 | A novel class of inhibitors for steroid 5α-reductase:Synthesis and evaluation of umbelliferone derivatives显示文摘 | Fan G J Mar W Park M K | 2001 | Bioorg Med Chem Lett2001，11，17： | 1 |
| 3 | Tread depth effects on tire rolling resistance 显示文摘 | Luchini J R Motil M M Mars W V | 2001 | Tire Science and Technology2001，29，3： | 1 |
| 4 | Price smarter on the net显示文摘 | Baker W Mar M Zawada C | 2001 | Harvard Business Review2001，79，2： | 1 |
| 5 | Composite sam- pling of highway runoff显示文摘 | CLARK D L ASCE A M MAR B W | 1981 | Journal of Environmental Engi- neering ?1981，107，5： | 1 |
| 6 | Price smarter on the net显示文摘 | Baker W Mar M Zawada C | 2001 | Harvard Business Review2001，79，2： | 1 |
| 7 | Tissue - type plasminogen ac-tivator promotes murine myofibroblast activation through LDL re-ceptor -related protein 1 - mediated integrin signaling 显示文摘 | HU K WU C MARS W M | 2007 | J ClinInvest2007，117，12： | 1 |
| 8 | Emergence of a clinical daptomycin-resistant Staphylococcus aureus isolate during treatment of methicillin-resistant Staphylococcus aureus bacteremia and osteomyelitis 显示文摘 | Marly F M Yeh W W Wennersten C B | 2006 | J Clin Microbiol2006，44，： | 1 |
| 9 | Signals and cells involved in regulating liver regeneration显示文摘 | Kang L-I Mars W M Michalopoulos G K | 2012 | Cells2012，1，4： | 1 |
| 10 | A Method for the Determination of Betaine in Tissues Using High Performance Liquid Chromatography显示文摘 | MAR H M H RIDKY W T GARNER C S | 1995 | Journal of Nutritional Biochemistry1995，6，7： | 1 |
| 11 | Tread depth effects on tire rolling resistance显示文摘 | Luchini J R Motil M M Mars W W | 2001 | Tire Science and Technology2001，29，3： | 1 |
| 12 | Local existence of dynamical and trapping horizons 显示文摘 | AnderssonL Mars M Simon W | 2005 | Phys Rev Lett2005，95，11： | 1 |
| 13 | A randomised,placebo-controlled,double-blind trial of the effects of d-methylphenidate on fatigue and cognitive dysfunction in women undergoing adjuvant chemotherapy for breast cancer显示文摘 | MAR FAN H G CLEMONS M XU W | 2008 | Support Care Cancer2008，16，6： | 1 |
| 14 | Phenobarbital suppressesgrowth and accelerates restoration ofdiferentiation markers of primary culture rat hepatacyeles in the chemically defined hepatocyte growth medium containiing hepatacyte growth factor and epidermal growth factor显示文摘 | Miyazaki M Mars W M Runge D | 1998 | Exp Cell Res1998，241，2： | 1 |
| 15 | The evaluation of twomanagement strategies for the Gulf of Alaska walleyepollock fishery under climate change显示文摘 | A'mar Z T Punt A E Dorn M W | 2009 | ICES Journal ofMarine Science2009，66，7： | 1 |
| 16 | Quantitative analysis of 59-tetrahydrocannabinol in preserved oral fluid by liquid chromatography-tandem mass spectrometry显示文摘 | LALOUP M MARIA del MAR R F W(X)D M | 2005 | Journal of Chromatography A2005，1082，： | 1 |
| 17 | Energy release rate of small cracks in hyperelastic materials 显示文摘 | At-Bachir M Mars W V Verron E | 2012 | International Journal of Non-Linear Mechanics2012，47，： | 1 |
| 18 | Total antioxidant activity and phenolic content in selected vegetables 显示文摘 | ISMAIL A MAR JAN Z M FOONG C W | 2004 | Food Chemistry2004，87，4： | 1 |
| 19 | Bioavailability of choline and choline esters from milk in rat pups显示文摘 | CHENG W L HOLMES-MCNARY M Q MAR M H | 1996 | J Nutr Biochem1996，7，： | 1 |
| 20 | A randomised,placebo-controlled,doubleblind trial of the effects of d-methylphenidate on fatigue and cognitive dysfunction in women undergoing adjuvant chemotherapy for breast cancer显示文摘 | Mar Fan HG Clemons M Xu W | 2008 | Support Care Cancer2008，16，： | 1 |
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