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| 1 | Carrier-free highly drug-loaded biomimetic nanosuspensions encapsulated by cancer cell membrane based on homology and active targeting for the treatment of glioma显示文摘Nanosuspensions,as a new drug delivery system for insoluble drugs,are only composed of a drug and a small amount of stabilizer,which is dispersed in an aqueous solution with high drug-loading,small particle size,high dispersion,and large specific surface area.It can significantly improve the dissolution,bioavailability,and efficacy of insoluble drugs.In this study,paclitaxel nanosuspensions((PTX)NS)were prepared by an ultrasonic precipitation method,with the characteristics of simple preparation and easy repetition.With the help of a homologous targeting mechanism,a kind of glioma C6 cancer cell membrane(CCM)-coated(PTX)NS was developed and modified with DWSW peptide to obtain DWSW-CCM-(PTX)NS with the functions of BBB penetration and tumor targeting.The results showed that the cancer cell membrane could effectively camouflage the nanosuspensions so that it was not cleared by the immune system and could cross the blood-brain-barrier(BBB)and selectively target tumor tissues.Cell uptake experiments and in vivo imaging confirmed that the uptake of DWSW-CCM-(PTX)NS by tumor cells and the distribution in intracranial gliomas increased.Cytotoxicity test and in vivo anti-glioma studies showed that DWSW-CCM-(PTX)NS could significantly inhibit the growth of glioma cells and significantly prolong the survival time of glioma-bearing mice.Finally,the cancer cell membrane coating endowed the nanosuspensions with the biological properties of homologous adhesion and immune escape.This study provides an integrated solution for improving the targeting of nanosuspensions and demonstrates the encouraging potential of biomimetic nanosuspensions applicable to tumor therapy. | Yueyue Fan Yuexin Cui Wenyan Hao Mengyu Chen Qianqian Liu Yuli Wang Meiyan Yang Zhiping Li Wei Gong Shiyong Song Yang Yang Chunsheng Gao | 2021 | Bioactive Materials2021，6，12： | 3 |
| 2 | Cholera Caused by a New Clone of Serogroup O1 Vibrio cholerae-Beijing Municipality,China,June 2021显示文摘Several lineages have been identified in the population of serogroup O1 Vibrio cholerae(V.cholerae)(1-3).The strains,which were responsible for the ongoing seventh cholera pandemic,were in Lineage 2.Nearly all the V.cholerae strains in this lineage carried genes coding cholera toxin(ctxAB)(1-2).Lineage 3b consists of strains isolated from different continents and the vast majority of strains in this lineage lack the ctxAB genes. | Hanqiu Yan Bo Pang Xin Lu Zhiyong Gao Pan Lu Xin Zhang Mengyu Wang Lingyu Shen Wenxuan Zhao Jianhong Zhao Weili Liang Lei Jia Haijian Zhou Zhigang Cui Xiaoli Du Biao Kan Quanyi Wang | 2022 | China CDC weekly2022，4，2： | 3 |
| 3 | NCL-based mitochondrial-targeting fluorescent probe for the detection of Glutathione in living cells显示文摘Glutathione(GSH) plays a critical role in maintaining cellular redox homeostasis in biological system.Mitochondrion is a pivotal organelle for cellular aerobic respiration and its disorder is associated with impaired redox balance, leading to cell death. In this work, we designed and synthesized a non-invasive“off-on” mitochondrial-targeting fluorescent probe QZ for the detection of GSH in living cells. Based on the mechanism of native chemical ligation(NCL) and fluorescence resonance energy transfer(FRET), a rhodamine B derivative, QZ was prepared, by choosing aromatic thioester bond as the selective reaction site. QZ exhibited excellent detection capability for GSH over Cys and Hcy. Upon addition of GSH to QZ solution, a remarkably enhanced fluorescence was observed with a limit of detection of 2.98 μmol/L.Furthermore, QZ was found to possess the specific mitochondrial localization ability in cell imaging experiments. Moreover, with exogenous and endogenous stimulations, QZ could image GSH in living cells. | Tongxia Jin Mengyu Cui Dan Wu Weiping Zhu Yufang Xu Xuhong Qian | 2021 | Chinese Chemical Letters2021，32，12： | 1 |
| 4 | The molecular pathogenesis of achalasia:a paired lower esophageal sphincter muscle and serum 4D label-free proteomic study显示文摘Background:Achalasia is a primary esophageal motility disorder with potential molecular pathogenesis remaining uncertain.This study aimed to identify the differentially expressed proteins and potential pathways among achalasia subtypes and controls to further reveal the molecular pathogenesis of achalasia.Methods:Paired lower esophageal sphincter(LES)muscle and serum samples from 24 achalasia patients were collected.We also collected 10 normal serum samples from healthy controls and 10 normal LES muscle samples from esophageal cancer patients.The 4D label-free proteomic analysis was performed to identify the potential proteins and pathways involved in achalasia.Results:Analysis of Similarities showed distinct proteomic patterns of serum and muscle samples between achalasia patients and controls(both P<0.05).Functional enrichment analysis suggested that these differentially expressed proteins were immunity-,infection-,inflammation-,and neurodegeneration-associated.The mfuzz analysis in LES specimens showed that proteins involved in the extracellular matrix–receptor interaction increased sequentially between the control group,type III,type II,and type I achalasia.Only 26 proteins altered in the same directions in serum and muscle samples.Conclusions:This first 4D label-free proteomic study of achalasia indicated that there were specific protein alterations in both the serum and muscle of achalasia,involving immunity,inflammation,infection,and neurodegeneration pathways.Distinct protein clusters between types I,II,and III revealed the potential molecular pathways associated with different disease stages.Analysis of proteins changed in both muscle and serum samples highlighted the importance of further studies on LES muscle and revealed potential autoantibodies. | Songfeng Chen Xiangbin Xing Xun Hou Qianjun Zhuang Niandi Tan Yi Cui Jinhui Wang Mengyu Zhang Shixian Hu Yinglian Xiao | 2023 | Gastroenterology Report2023，11，1： | 0 |
| 5 | Clinical outcomes of asymptomatic low-grade esophagitis:results from a multicenter Chinese cohort显示文摘Background:Asymptomatic low-grade(Los Angeles Classification Grades A and B)esophagitis is common in clinical practice with unclear clinical outcomes.This study aimed to explore the clinical outcomes of asymptomatic low-grade esophagitis.Methods:This was a multicenter cohort study conducted by three academic hospitals in China.Asymptomatic low-grade esophagitis patients between January 2015 and December 2019 were included.Mucosal healing condition 1year after initial diagnosis,symptomoutcomes,and proton-pump inhibitor(PPI)use within 1year after initial diagnosis were studied and compared.Results:A total of 248 asymptomatic low-grade esophagitis patients were included.Esophagitis disappeared in 76.2%of patients 1 year after initial diagnosis.In terms of symptom outcomes,89.9%of patients did not present gastroesophageal reflux disease(GERD)symptoms within 1 year after initial diagnosis.No significant difference was found in the proportion of patients who presented GERD symptoms and in the proportion of patients with persistent esophagitis between the PPI group and the non-PPI group(all P>0.05).Patients with initial Grade B esophagitis were more likely to present follow-up GERD symptoms(16.0%vs 7.5%,P=0.041)and had more severe follow-up esophagitis than those with Grade A(P<0.001).Patients with follow-up GERD symptoms were more likely to have persistent esophagitis than those without.Conclusions:This study demonstrated that asymptomatic low-grade esophagitis had relatively benign clinical outcomes.Patients with initial Grade B esophagitis and patients with follow-up GERD symptoms were more likely to be those who are in genuine need of further follow-up and treatments. | Songfeng Chen Xuelian Xiang Xiaohao Zhang Qianjun Zhuang Niandi Tan Xun Hou Mengyu Zhang Junnan Hu Chaofan Duan Yi Cui Jinhui Wang Xiangbin Xing Nina Zhang Yinglian Xiao | 2022 | Gastroenterology Report2022，10，1： | 0 |
| 6 | CCDC189 affects sperm flagellum formation by interacting with CABCOC01显示文摘Multiple morphological abnormalities of the sperm flagella(MMAF)are one of the major causes of male infertility and are characterized by multiple defects.In this study,we found that the coiled-coil domain-containing 189(Ccdc189)gene was predominantly expressed in mouse testes and that inactivation of the Ccdc189 gene caused male infertility.Histological studies revealed that most sperm from Ccdc189-deficient mice carried coiled,curved or short flagell a,which are typical MMAF phenotypes.Immunoelectron microscopy showed that the CCDC189 protein was located at the radial spoke of the first peripheral microtubule doublet in the sperm axoneme.A CCDC189-interacting protein,CABCOCO1(ciliary-associated calcium-binding coiled-coil protein 1),was discovered via co-immunoprecipitation and mass spectrometry,and inactivation of Cabcocol caused malformation of sperm flagell a,which was consistent with findings obtained with Ccdc189-deficient mice.Further studies revealed that inactivation of CCDC189 caused downregulation of CABCOCO1 protein expression and that both CCDC189 and CABCOCO1 interacted with the radial-spoke-specific protein RSPH1 and intraflagellar transport proteins.This study demonstrated that Ccdc189 is a radial-spoke-associated protein and is involved in sperm flagellum formation through its interactions with CABCOCO1 and intraflagellar transport proteins. | Mengyue Wang Junyan Kang Zhiming Shen Yingchun Hu Min Chen Xiuhong Cui Hongbin Liu Fei Gao | 2023 | National Science Review2023，10，9： | 0 |
| 7 | Reading-related Brain Function Restored to Normal After Articulation Training in Patients with Cleft Lip and Palate:An fMRI Study显示文摘Cleft lip and/or palate(CLP)are the most common craniofacial malformations in humans.Speech problems often persist even after cleft repair,such that follow-up articulation training is usually required.However,the neural mechanism behind effective articulation training remains largely unknown.We used fMRI to investigate the differences in brain activation,functional connectivity,and effective connectivity across CLP patients with and without articulation training and matched normal participants.We found that training promoted task-related brain activation among the articulation-related brain networks,as well as the global attributes and nodal efficiency in the functional-connectivity-based graph of the network.Our results reveal the neural correlates of effective articulation training in CLP patients,and this could contribute to the future improvement of the post-repair articulation training program. | Liwei Sun Wenjing Zhang Mengyue Wang Songjian Wang Zhen Li Cui Zhao Meng Lin Qian Si Xia Li Ying Liang Jing Wei Xu Zhang Renji Chen Chunlin Li | 2022 | Neuroscience Bulletin2022，38，10： | 0 |
| 8 | Study on Rural Landscape Planning -A Case Study of Yanbian Korean Autonomous Prefecture in China显示文摘 | Zheng Jiaqi Li Mengyu Cui Xiangri | 2017 | 当代农业(中英文版)2017，6，1： | 0 |
| 9 | Simultaneous determination of gallic acid and p-coumaric acid in rat plasma by UPLC-MS/MS and its application to a comparative pharmacokinetic study after oral administration of monomer compound and red wine extract显示文摘A rapid,sensitive and selective ultra high performance liquid chromatography-tandem mass spectrometry(UPLCMS/MS)method was developed and validated for simultaneous determination of gallic acid(GA)and p-coumaric acid(CA)in rat plasma.Plasma samples were extracted by methanol and separated on an ACQUITY UPLC BEH C18 column(1.7μm,100 mm×2.1 mm)using gradient elution consisting of acetonitrile–0.2%formic acid within a runtime of 4.0 min.The detection was performed in multiple reaction monitoring(MRM)mode with negative ionization.The linear range was 20–20000 ng/mL for both GA and CA,with lower limits of quantification of 20 ng/mL.Intra-day and inter-day precisions were within 5.4%and 10.0%,respectively and the accuracy(relative error,RE,%)was less than 7.2%and–4.9%,respectively.The mean absolute extraction recoveries of both analytes and IS from rat plasma were all more than 82.6%.The validated method was successfully applied to the comparative pharmacokinetic study of GA and CA in rat plasma after oral administration of GA and CA monomers and red wine extract,respectively.It was found that both the area under the curve(AUC)and t1/2 of the two constituents were remarkably increased for red wine extract group than that in monomer group,indicating the priority of intake of red wine to active component monomer. | Huijie Lv Mengyu Zou Weichao Yu Baoshan Sun Yan Cui | 2019 | Journal of Polyphenols2019，1，1： | 0 |
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