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| 1 | Proposal of an ultrasonographic classification for hepatic alveolar echinococcosis: Echinococcosis multilocularis Ulm classification-ultrasound显示文摘AIM: To establish an ultrasonographic classification based on a large sample of patients with confirmed hepatic alveolar echinococcosis(AE).METHODS: Clinical data and ultrasonography(US) findings of 185 patients(100 males; 85 females; mean age at diagnosis: 51.4 ± 17.6 years; mean age at time of US examination: 58.7 ± 18.2 years) were retrospectively reviewed with respect to the US morphology of hepatic AE lesions. The sonomorphological findings were grouped according to a five-part classification scheme.RESULTS: Application of the new classification resulted in the following distribution of sonomorphological patterns among the patients examined: hailstorm(54.1%); pseudocystic(13.5%); ossification(13.0%); hemangioma-like(8.1%); and metastasis-like(6.5%). Only 4.9% of lesions could not be assigned to a sonomorphological pattern.CONCLUSION: The sonomorphological classification proposed in the present study facilitates the diagnosis,interpretation and comparison of hepatic alveolar echinococcosis in routine practice and in the context of scientific studies. | Wolfgang Kratzer Beate Gruener Tanja EM Kaltenbach Sarina Ansari-Bitzenberger Peter Kern Michael Fuchs Richard A Mason Thomas FE Barth Mark M Haenle Andreas Hillenbrand Suemeyra Oeztuerk Tilmann Graeter | 2015 | World Journal of Gastroenterology2015，21，43： | 16 |
| 2 | 小麦胚乳14-3-3蛋白的表达及其与淀粉体淀粉合成酶的互作显示文摘从小麦胚乳中克隆了14-3-3基因,并将其分别插入pET29c和pET41c质粒,用热激法转化大肠杆菌BL21-CodonPlus(DE3)-RP,得到高效表达的蛋白,但融合蛋白主要以包涵体的形式存在。可溶性的融合蛋白可直接通过S-蛋白琼脂糖树脂纯化。包涵体经8molL-1尿素溶解变性,稀释复性后,结合到S-蛋白琼脂糖树脂上,也得到纯化的融合蛋白。复性后的融合蛋白对蔗糖合酶活性表现抑制作用,说明包涵体14-3-3融合蛋白恢复活性。将结合14-3-3融合蛋白的S-蛋白琼脂糖树脂作为诱饵与小麦胚乳淀粉体提取液进行亲和杂交,与14-3-3蛋白特异互作的淀粉合成酶结合到S-蛋白琼脂糖树脂上,Western检测结果表明,淀粉体淀粉合酶I(SSI)、淀粉合酶II(SSII)、淀粉分支酶Iia(SBEIIa)、淀粉分支酶Iib(SBEIIb)和ADP焦磷酸化酶大亚基(SH2)与14-3-3蛋白存在互作,而淀粉分支酶I(SBEI)、淀粉磷酸化酶(SP)、D-酶(DE)和ADP焦磷酸化酶小亚基(BT2)不能与14-3-3蛋白结合,说明小麦胚乳14-3-3蛋白对淀粉体淀粉合成具有一定的调控作用。 | 宋健民 戴双 李豪圣 刘爱峰 程敦公 楚秀生 Ian J TETLOW Michael J EMES | 2009 | 作物学报2009，35，8： | 5 |
| 3 | Aggressive surgical and medical management of autosoma recessive polycystic kidney disease (ARPKD) 显示文摘 | Joel MS Mark WB Michael EM | 1993 | Pediatr Urolo1993，42，3： | 1 |
| 4 | Left ventricular mass reliability of M-mode and 2-dimensional echocardiographic formulas显示文摘 | Saul GM Hugh EM Michael JW | 2002 | Hypertension2002，40，： | 1 |
| 5 | Rapid detection of bone metastasis at thoracoabdominal CT: accuracy and efficiency of a new visualization algorithm 显示文摘 | Daniel FT Michael T Marius EM | 2014 | Radiology2014，270，3： | 1 |
| 6 | Short versus standard duration oral antibiotic therapy for acute urinary tract infection in children 显示文摘 | Michael M Hodson EM Craig JC | 2003 | Cochrane Database Syst Rev2003，，00： | 1 |
| 7 | Sequestra in primary lyphoma of bone:prevalence and radiologic features显示文摘 | Michael EM Mark JK | 1993 | AJR1993，160，： | 1 |
| 8 | Effects of a medical emergency team on reduction of incidence of and mortality from unexpected cardiac arrests in hospital: preliminary study 显示文摘 | Buist MD Michael DB Gaye EM | 2002 | BMJ2002，3，24： | 1 |
| 9 | Nuclear factor-κappa B is activated in alveolar macrophages from patients with acute respiratory distress syndrome显示文摘 | Michael S Ernest EM Frederick AM | 1996 | Crit Care Med1996，24，8： | 1 |
| 10 | Short versus standard duration oralantibiotic therapy for acute urinary tract infection in children显示文摘 | Michael M Hodson EM Craig JC | 2003 | Cochrane Database Syst Rev2003，，00： | 1 |
| 11 | Insulin regulation of free fatty acid metabolism显示文摘 | Shon EM Sreekumaran N Michael DJ | 1999 | Diabetes1999，48，： | 1 |
| 12 | Scapular fracture 显示文摘 | Jesser RA Michael EM | 1991 | Clin Orthop1991，269，： | 1 |
| 13 | Short compared with standard duration of antibiotic treatment for urinary tract infection : A systematic review of randomized controlled trials 显示文摘 | Michael M Hodson EM Craig JC | 2002 | Arch Dis Child2002，87，2： | 1 |
| 14 | Intermediate outcomes after the Fontan procedure in the current era显示文摘 | Michael EM Richard FI William G | 2006 | J Thoracic Cardiovasc Surg2006，131，1： | 1 |
| 15 | Intermediate outcomes after the Fontan procedure in the current era 显示文摘 | Michael EM Richard FI William G | 2006 | J Thoracic Cardiovasc Surg2006，131，1： | 1 |
| 16 | The three R' s of lung health and disease: repair, remodeling, and regeneration显示文摘 | Michael FB Edward EM | 2011 | J Clin Invest2011，121，： | 1 |
| 17 | Outcomes of minimally invasive parathyroidectomy in pediatric patients with primary hyperparathyroidism owing to parathyroid adenoma:a single institution experience显示文摘 | Edna EM Michael A Levine N | 2016 | Journal of Pediatric Surgery2016，4，3： | 1 |
| 18 | Social-emotional delays at 2 years in extremely low gestational age survivors:Correlates of impaired orientation/engagement and emotional regulation显示文摘 | Lauren AC Michael EM O'Shea TM | 2013 | Early Human Development2013，11，89： | 1 |
| 19 | Solvent effects on copper nanoparticle growth behavior in AOT reverse micelle system 显示文摘 | Joanna PC Michael EM Jason BT | 2001 | J Phys Chem B2001，105，： | 1 |
| 20 | Postinjury coagulopa thy management goal directed resuscitation via POC throm belastography显示文摘 | J effry LK Ernest EM Michael S | 2010 | Ann Surg2010，251，4： | 1 |
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