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| 1 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 2 | Rationalizing prescribing for older patients with multimorbidity:considering time to benefit显示文摘 | Holmes HM Min LC Yee M | 2013 | Drugs Aging2013，30，9： | 1 |
| 3 | Rationalizing prescribing for older patients with multimorbidity , considering time to benefit显示文摘 | Holmes HM Min LC Yee M | 2013 | Drugs Aging2013，30，9： | 1 |
| 4 | Reversible posterior leukoenceph- alopathy in connective tissue diseases 显示文摘 | Min L Zwerling J Ocava LC | 2006 | Semln Arthritis Rheum2006，35，6： | 1 |
| 5 | HIV drug resistance detec- ted during low-level viraemia is associated with subsequent virologic failure显示文摘 | Swenson LC Min JE Woods CK | 2014 | AIDS2014，28，8： | 1 |
| 6 | ECG beat classification using PCA,LDA,ICA and discrete wavelet transform显示文摘 | Martis RJ Rajendra Acharya U Min LC | 2013 | Biomedical Signal Processing and Control2013，8，5： | 1 |
| 7 | Reversible posterior leukoencephalopathy in connective tissue diseases显示文摘 | Min L Zwerling J Oeava LC | 2006 | Semin Arthritis Rheum2006，35，6： | 1 |
| 8 | Reversible posterior leukoencephslopathy in connective tissue diseases显示文摘 | Min L Zwerling J Ocava LC at al | 2006 | Semin Arthritis Rheum2006，35，： | 1 |
| 9 | Reversible posterior leu- koencephalopathy in connective tissue diseases 显示文摘 | Min L Zwerling J Ovava LC | 2006 | Semin Arthritis Rhenm2006，35，6： | 1 |
| 10 | Medial foot loading on ankle and knee biomechanics 显示文摘 | Mitchell LC Ford KR Minning S | 2008 | N Am J Sports Phys Ther2008，3，3： | 1 |
| 11 | Effect of medial foot loading pattern on ankle and knee landing biomechanics显示文摘 | Mitchell LC Ford KR Minning SJ | | 0，，03： | 1 |
| 12 | Reversible posterior leukoencephalopathy in connective tissue diseases显示文摘 | Min L Zwerling J Oeava LC | 2006 | Semin Arthritis Rheum2006，35，6： | 1 |
| 13 | Reversible posterior leukoencephalopathy in connective tissue diseases 显示文摘 | Min L Zwerling J Ocava LC | 2006 | Semin Arthritis Rheum2006，35，6： | 1 |
| 14 | Reversible posterior leukoencephalopathy in connective tissue diseases显示文摘 | Min L Zwerling J Ocava LC | 2006 | Semin Arthritis Rdaeum2006，35，6： | 1 |
| 15 | Reversible posterior leukoencephalopathy in connective tissue diseases显示文摘 | Min L Zwerling J Ocava LC | 2006 | Semin Arthritis Rheum2006，35，6： | 1 |
| 16 | Purification, physieochemical and immunological characterization of arginine kinase, an aller- gen of crayfish(Procambarus clarkii)显示文摘 | Heng LC Hal YM Min JC | 2013 | Food Chem Toxicol2013，62，： | 1 |
| 17 | Reversible posterior leukoencephalopathy in connective tissue diseases 显示文摘 | Min L Zwerling J Ocava LC | 2006 | Semin Arthritis Rheum2006，35，6： | 1 |
| 18 | Reversible posterior leukoencephalopathy in connective tissue diseases显示文摘 | Zwerling J Ocava LC | 2006 | Semin Arthritis Rheum2006，35，6： | 1 |
| 19 | Reversible posterior leukoeneephalopathy in connective tissue diseases 显示文摘 | Min L Zwerling J Ocava LC | 2006 | Semin Arthritis Rheum2006，35，6： | 1 |
| 20 | Reversible posterior leukoencephalopathy in connective tissue diseases显示文摘 | Min L Zerling J Ocava LC | 2006 | Semin Arthritis Rheum2006，35，6： | 1 |
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