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4篇 您的检索式：作者名="MingNi"

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		题名	作者	年代	出处	被引量
	1	Human scFv antibody fragments specific for hepatocellular carcinoma selected from a phage display library显示文摘AIM: To identify the scFv antibody fragments specific for hepatocellular carcinoma by biopanning from a large human naive scFv phage display library.METHODS: A large human naive scFv phage library was used to search for the specific targets by biopanning with the hepatocellular carcinoma cell line HepG2 for the positiveselecting and the normal liver cell line L02 for the counterselecting. After three rounds of biopanning, individual scFv phages binding selectively to HepG2 cells were picked out. PCR was carried out for identification of the clones containing scFv gene sequence. The specific scFv phages were selected by ELISA and flow cytometry. DNA sequences of positive clones were analyzed by using Applied Biosystem Automated DNA sequencers 3 730. The expression proteins of the specific scFv antibody fragments in E. coli HB2151were purified by the affinity chromatography and detected by SDS-PAGE, Western blot and ELISA. The biological effect of the soluble antibody fragments on the HepG2 cells was investigated by observing the cell proliferation.RESULTS: Two different positive clones were obtained and the functional variable sequences were identified.Their DNA sequences of the scFv antibody fragments were submitted to GenBank (accession nos: AY686498 and AY686499). The soluble scFv antibody fragments were successfully expressed in E. coli HB2151. The relative molecular mass of the expression products was about 36 ku,according to its predicted Mr value. The two soluble scFv antibody fragments also had specific binding activity and obvious growth inhibition properties to HepG2 cells.CONCLUSION: The phage library biopanning permits identification of specific antibody fragments for hepatocellular carcinoma and affords experiment evidence for its immunotherapy study.	BingYu MingNi Wen-HanLi PingLei WeiXing Dai-WenXiao YuHuang Zhen-JieTang Hui-FenZhu Guan-XinShen	2005	World Journal of Gastroenterology2005，11，26：	2
	2	Novel image copy detection with rotating tolerance显示文摘	Wu Mingni Lin Chachen Chang Chinchen	2007	Journal of Systems and Software2007，80，7：	1
	3	Online risk based security as sessment显示文摘	MingNi MeCalley JD Vittal V	2003	IEEE Trans on Power Systems2003，18，1：	1
	4	Novel image copy detection with rotating tolerance显示文摘	Lin Chiachen Wu Mingni Chang Chinchen	2007	Journal of Systems and Software2007，80，7：	1

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