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| 1 | Efficient production of chemicals from microorganism by metabolic engineering and synthetic biology显示文摘The use of traditional chemical catalysis to produce chemicals has a series of drawbacks,such as high dependence on fossil resources,high energy consumption,and environmental pollution.With the development of synthetic biology and metabolic engineering,the use of renewable biomass raw materials for chemicals synthesis by constructing efficient microbial cell factories is a green way to replace traditional chemical catalysis and traditional microbial fermentation.This review mainly summarizes several types of bulk chemicals and high value-added chemicals using metabolic engineering and synthetic biology strategies to achieve efficient microbial production.In addition,this review also summarizes several strategies for effectively regulating microbial cell metabolism.These strategies can achieve the coupling balance of material and energy by regulating intracellular material metabolism or energy metabolism,and promote the efficient production of target chemicals by microorganisms. | Yang Zhang Jing Yu Yilu Wu Mingda Li Yuxuan Zhao Haowen Zhu Changjing Chen Meng Wang Biqiang Chen Tianwei Tan | 2021 | Chinese Journal of Chemical Engineering2021，34，2： | 2 |
| 2 | Topological Comparison and Analysis of Medium-voltage and High-power Direct-linked PV Inverter显示文摘Among all the renewable energy sources,the installed capacity of solar power generation is the fastest growing in recent years,so photovoltaic(PV)power generation still has great market potential.Compared with low-power systems,large-scale PV systems are more commercially attractive,because they can reduce the cost of the system per watt.The PV inverters with centralized and string structure have been applied in large-scale PV plant,but it is difficult to further increase the voltage and power levels for a single converter.In addition,the line-frequency isolation transformer requires a large amount of materials and has a large volume and weight.Therefore,it is a current trend for large-scale PV system to increase the voltage and power levels to directly connect to the medium-voltage power grid.Based on this,this paper investigates and compares several topologies of PV inverters without line-frequency transformer,including the MMC structure and the three-phase cascaded H-bridge(CHB)structure,which are able to directly connect to the 35kV medium-voltage power grid,and can not only make the voltage and power levels higher,but also further reduce the cost and volume of the whole system. | Xing Zhang Mingda Wang Tao Zhao Wang Mao Yuhua Hu Renxian Cao | 2019 | CES Transactions on Electrical Machines and Systems2019，3，4： | 2 |
| 3 | Urea reactor integrity evaluation based on failure analysis显示文摘 | Song Mingda Wang Weiqiang Zhao Yafang | 2007 | Journal of Pressure Vessel Technology2007，129，4： | 1 |
| 4 | Application of Functionalized N,S-Ketene Acetals Microwave- Assisted Three-Component Domino Reaction for Rapid Direct Access to Imidazo[1,2-a]pyridines显示文摘 | Li, Ming Li, Tengfei Zhao, Kelong Wang, Mingda Wen, Lirong | 2013 | Chinese Journal of Chemistry2013，31，8： | 0 |
| 5 | Experimental Investigation of Local Scour Around Artificial Reefs in Steady Currents显示文摘Artificial reef is a man-made object that is deployed purposefully on the seafloor to restore the offshore fishery resources and the ecological environment.To secure its ecological effects,it is important to study the possible instability of artificial reefs,like drifting and reversing caused by burial and scour in different seafloor conditions.In the present study,experiments of local scour around an artificial reef are carried out in steady currents.The effect of the open-area ratios and the open-hole heights of the cubic reefs,and the bottom angles of the triangular reefs on the time-scale of the scour process and the equilibrium scour depth are investigated.The results indicate that for the cubic artificial reef,the scour depth decreases with the increasing open-area ratios,and increases with the increasing open-hole heights.In the present study,the optimal prototype of the cubic reef with an open-area ratio of 0.49 and open-height of 0.7 m produces the minimum scour depth.For the triangular reef,the scour depth increases when the bottom angle increases.Moreover,based on the experimental results,empirical equations of the effects of the cut-opening and the bottom angle on the maximum equilibrium scour depth are proposed.The formulas will provide theoretical support and practical guidance for the optimized design and construction of artificial reefs. | TANG Yanli WEI Shuangwu YANG Mingda WANG Xinxin ZHAO Fenfang | 2022 | Journal of Ocean University of China2022，21，2： | 0 |
| 6 | Carrying Railway 5G Service Application by Multi-technology Convergence显示文摘The application scenarios of railway radio communication are complex,while the existing narrowband communication such as GSM-R and radio train dispatching system fails to meet the requirements of service application development.The carrying capacity of railway 5G-R has been greatly improved,mainly for wide-area train-ground communication services such as dispatching communication,train control and train operation safety monitoring.However,for large passenger stations,freight yards,maintenance bases and other places with densely populated railway operators,it is unable to carry overall railway service applications limited by the spectrum bandwidth of 5G private networks,so it is necessary to consider various radio technologies for supplementation.This paper also elaborates the railway service application scenarios,analyzes the characteristics of radio communication technologies such as 5G mmWave and the 6th-generation wireless LAN technology(Wi-Fi6),and discusses the schemes of multi-technology convergence carrying railway 5G service applications. | YANG Rui GUO Guifang ZHAO Wuyuan ZHENG Mingda | 2023 | Chinese Railways2023，，1： | 0 |
| 7 | Conceptual design and analysis of legged landers with orientation capability显示文摘The lander with orientation capability is of fundamental importance for the“Returning”mission because its body can be the base with a suitable launching angle for the ascender lifting off.This paper proposes the conceptual design and verification of Landers with Orientation Capability(LOCs).Firstly,the topological composition of the lander is analyzed and expressed in the form of Lie group,which provides the fundamental principle for the type synthesis of LOCs.Then,the type synthesis approach for designing LOCs is proposed,which includes designing the Equivalent Parallel Mechanism(EPM)and the auxiliary limbs.Numerous mechanisms of LOCs with twodimensional rotational motions are obtained based on the type synthesis approach by developing and combining the EPMs and auxiliary limbs.Afterward,kinematics and Jacobian matrix of a typical legged lander are developed,the workspace is analyzed,singularity configurations are analyzed based on the wrench graph method,and finally,the orientation capability is analyzed.The pitch and yaw angle reach 17.5°,considering the permissible range of joints.The proposed types of LOCs offer potential candidate for the“Returning”mission of the exploration mission. | Rongfu LIN Weizhong GUO Changjie ZHAO Mingda HE | 2023 | Chinese Journal of Aeronautics2023，36，3： | 0 |
| 8 | Plant Membrane Transport Research in the Post-genomic Era显示文摘Membrane transport processes are indispensable for many aspects of plant physiology including mineral nutrition,solute storage,cell metabolism,cell signaling,osmoregulation,cell growth,and stress responses.Completion of genome sequencing in diverse plant species and the development of multiple genomic tools have marked a new era in understanding plant membrane transport at the mechanistic level.Genes coding for a galaxy of pumps,channels,and carriers that facilitate various membrane transport processes have been identified while multiple approaches are developed to dissect the physiological roles as well as to define the transport capacities of these transport systems.Furthermore,signaling networks dictating the membrane transport processes are established to fully understand the regulatory mechanisms.Here,we review recent research progress in the discovery and characterization of the components in plant membrane transport that take advantage of plant genomic resources and other experimental tools.We also provide our perspectives for future studies in the field. | Ren-Jie Tang Mingda Luan Chao Wang Dhondup Lhamo Yang Yang Fu-Geng Zhao Wen-Zhi Lan Ai-Gen Fu Sheng Luan | 2020 | Plant Communications2020，1，1： | 0 |
| 9 | Corrigendum to“Bioinspired supramolecular nanofiber hydrogel through self-assembly of biphenyl-tripeptide for tissue engineering”[Bioact.Mater.8(2022)396-408]显示文摘The authors regret that the published version of the above article contained error in Fig.6F,which was not identified during the proofing stage.The wrong live/dead stain,F-actin stain,and SEM images of BPAA-GFF gel were selected during the assembly of Fig.6F.The images of Fig.6F have been replaced with the correct images as follow.The authors apologize for this error and state that the figure’s correction did not change the scientific conclusions of the article in any way. | Yong Sun Xing Li Mingda Zhao Yafang Chen Yang Xu Kefeng Wang Shaoquan Bian Qing Jiang Yujiang Fan Xingdong Zhang | 2023 | Bioactive Materials2023，，5： | 0 |
| 10 | Therapeutic sponge prevents postoperative breast cancer recurrence by sustainably dissociating into CD44-targeted nanoplatform显示文摘Locoregional recurrence and distant metastasis of breast cancer still pose a significant risk for patients’survival.To address the clinical challenge,functional absorbable sponges(HA-SH/PP-Dox/Lap/COL I(HCNPs))were constructed by biomimetic extracellular matrix of collagen I/hyaluronic acid complex conjugated with doxorubicin/lapatinib(Dox/Lap)-loaded nanoparticles.The HCNPs sponge exhibited excellent clotting ability and blood absorption rate.Worthily,Dox/Lap-loaded nanoparticles were synchronously endowed with a large number of oligo hyaluronic acid segments after degradation,which thus enhanced the ability of targeting into CD44-overexpressed tumor cells.The implantable HCNPs sponge in resected cavity of postoperative 4T1 models inhibited the spread of scattered tumor cells by absorbing the inevitable bleeding.More importantly,CD44 targeted nanoparticle with suitable Dox/Lap proportion continuously released from sponge to kill tumor cells of surrounding HCNPs and those remaining at surgical margin,thus prevented local recurrence as well as distant metastasis.Therefore,the functional HCNPs sponge might provide a safer and more effective strategy for postoperative treatment of cancer. | Junhui Sui Mingda Zhao Zhihao Guo Jiafeng Li Jie Chen Hongli Chen Jie Liang Yong Sun Xingdong Zhang Yujiang Fan | 2024 | Nano Research2024，17，3： | 0 |
| 11 | Silencing of the Nonspecific Phospholipase C6(NPC6)Gene Induces Ricinoleic Acid Accumulation in Castor Seeds显示文摘Castor,scientifically known as Ricinus communis L.,is among the top ten oil crops globally.It is considered a renewable resource and is commonly referred to as‘green oil’.Castor seeds contain castor oil as their main component,which is predominantly composed of ricinoleic acid.This study utilized RNAi technology to silence the NPC6 gene in NO.2129 castor,resulting in the creation of mutant plants L1 and L2.The weight of 100 dry seed kernels from L1 and L2 exceeds that from NO.2129.The crude fat and ricinoleic acid levels of L1 and L2 were higher than those of NO.2129 at various developmental stages.In the proteomics analysis of 60-day-old castor seeds,a total of 21 differentially expressed proteins were identified,out of which 19 were successfully recognized.Eleven of the differentially expressed proteins identified were legumins,which play a crucial role in nutrient storage within the seed.Silencing the NPC6 gene results in the accumulation of ricinoleic acid in castor seeds.The findings of this study not only enhance our knowledge of NPC6’s role in regulating castor seed oil synthesis but also offer fresh perspectives for investigating oil synthesis and accumulation in other plant species. | Yong Zhao Lili Li Rui Luo Mu Peng Tongtong Jiang Mingda Yin Yanpeng Wen Zihan Wang Fenglan Huang Fanjuan Meng | 2023 | Phyton-International Journal of Experimental Botany2023，92，12： | 0 |
| 12 | A SPX domain vacuolar transporter links phosphate sensing to homeostasis in Arabidopsis显示文摘Excess phosphate(Pi)is stored into the vacuole through Pi transporters so that cytoplasmic Pi levels remain stable in plant cells.We hypothesized that the vacuolar Pi transporters may harbor a Pi-sensing mechanism so that they are activated to deliver Pi into the vacuole only when cytosolic Pi reaches a threshold high level.We tested this hypothesis using Vacuolar Phosphate Transporter 1(VPT1),a SPX domain-containing vacuolar Pi transporter,as a model.Recent studies have defined SPX as a Pi-sensing module that binds inositol polyphosphate signaling molecules(InsPs)produced at high cellular Pi status.We showed here that Pi-deficient conditions or mutation of the SPX domain severely impaired the transport activity of VPT1.We further identified an auto-inhibitory domain in VPT1 that suppresses its transport activity.Taking together the results from detailed structure-function analyses,our study suggests that VPT1 is in the auto-inhibitory state when Pi status is low,whereas at high cellular Pi status InsPs are produced and bind SPX domain to switch on VPT1 activity to deliver Pi into the vacuole.This thus provides an auto-regulatory mechanism for VPT1-mediated Pi sensing and homeostasis in plant cells. | Mingda Luan Fugeng Zhao Guangfang Sun Min Xu Aigen Fu Wenzhi Lan Sheng Luan | 2022 | Molecular Plant2022，15，10： | 0 |
| 13 | Bioinspired supramolecular nanofiber hydrogel through self-assembly of biphenyl-tripeptide for tissue engineering显示文摘Supramolecular nanofiber peptide assemblies had been used to construct functional hydrogel biomaterials and achieved great progress.Here,a new class of biphenyl-tripeptides with different C-terminal amino acids sequences transposition were developed,which could self-assemble to form robust supramolecular nanofiber hydrogels from 0.7 to 13.8 kPa at ultra-low weight percent(about 0.27 wt%).Using molecular dynamics simulations to interrogate the physicochemical properties of designed biphenyl-tripeptide sequences in atomic detail,reasonable hydrogen bond interactions and“FF”brick(phenylalanine-phenylalanine)promoted the formation of supramolecular fibrous hydrogels.The biomechanical properties and intermolecular interactions were also analyzed by rheology and spectroscopy analysis to optimize amino acid sequence.Enhanced L929 cells adhesion and proliferation demonstrated good biocompatibility of the hydrogels.The storage modulus of BPAA-AFF with 10 nm nanofibers self-assembling was around 13.8 kPa,and the morphology was similar to natural extracellular matrix.These supramolecular nanofiber hydrogels could effectively support chondrocytes spreading and proliferation,and specifically enhance chondrogenic related genes expression and chondrogenic matrix secretion.Such biomimetic supramolecular short peptide biomaterials hold great potential in regenerative medicine as promising innovative matrices because of their simple and regular molecular structure and excellent biological performance. | Yong Sun Xing Li Mingda Zhao Yafang Chen Yang Xu Kefeng Wang Shaoquan Bian Qing Jiang Yujiang Fan Xingdong Zhang | 2022 | Bioactive Materials2022，7，2： | 0 |
| 14 | Proteomic Analyses of Three Inflorescence Styles of Castor(Ricinus communis L.)at Different Developmental Stages显示文摘Castor(Ricinus communis L.)is one of ten oil crops in the world and has complex inflorescence styles.Generally,castor has three inflorescence types:single female inflorescence(SiFF),standard female inflorescence(StFF)and bisexual inflorescence(BF).StFF is realized as a restorer line and as a maintainer line,which was applied to castor hybrid breeding.However,the developmental mechanism of the three inflorescences is not clear.Therefore,we used proteomic techniques to analyze different inflorescence styles.A total of 72 diferentially abundant protein species(DAPs)were detected.These DAPs are primarily involved in carbon and energy metabolism and carbon fixation in the photosynthetic organism pathway.The results showed that DAPs are involved in photosynthesis to control the distribution of imported carbohydrates and exported photoassimilates and thus affect the inflorescence development of castor.In addition,these DAPs are also involved in cysteine and methionine metabolism.Quantitative real-time PCR(qRT-PCR)results demonstrated that the proteomics data collected in this study were reliable.Our findings indicate that the carbon cycle and amino acid metabolism influence the inflorescence development of castor. | Xue Lei Yong Zhao Rui Luo Mingda Yin Yanpeng Wen Zhiyan Wang Xuemei Hu Fenglan Huang | 2023 | Phyton-International Journal of Experimental Botany2023，92，5： | 0 |
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