|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Improvement of oxytetracycline production mediated via cooperation of resistance genes in Streptomyces rimosus显示文摘Increasing the self-resistance levels of Streptomyces is an effective strategy to improve the production of antibiotics.To increase the oxytetracycline(OTC) production in Streptomyces rimosus,we investigated the cooperative effect of three co-overexpressing OTC resistance genes:one gene encodes a ribosomal protection protein(otrA) and the other two express efflux proteins(otrB and otrC).Results indicated that combinational overexpression of otrA,otrB,and otrC(MKABC) exerted a synergetic effect.OTC production increased by 179%in the recombinant strain compared with that of the wild-type strain M4018.The resistance level to OTC was increased by approximately two-fold relative to the parental strain,thereby indicating that applying the cooperative effect of self-resistance genes is useful to improve OTC production.Furthermore,the previously identified cluster-situated activator OtcR was overexpressed in MKABC in constructing the recombinant strain MKRABC;such strain can produce OTC of approximately7.49 g L^((-1)),which represents an increase of 19%in comparison with that of the OtcR-overexpressing strain alone.Our work showed that the cooperative overexpression of self-resistance genes is a promising strategy to enhance the antibiotics production in Streptomyces. | Shouliang Yin Xuefeng Wang Mingxin Shi Fang Yuan Huizhuan Wang Xiaole Jia Fang Yuan Jinliang Sun Tiejun Liu Keqian Yang Yuxiu Zhang Keqiang Fan Zilong Li | 2017 | Science China(Life Sciences)2017，60，9： | 10 |
| 2 | Science and Prediction of Heavy Rainfall over China:Research Progress since the Reform and Opening-Up of New China显示文摘This paper reviews the major progress on development of the science and prediction of heavy rainfall over China since the beginning of the reform and opening-up of new China(roughly between 1980 and 2019).The progress of research on the physical mechanisms of heavy rainfall over China is summarized from three perspectives:1)the relevant synoptic weather systems,2)heavy rainfall in major sub-regions of China,and 3)heavy rainfall induced by typhoons.The development and application of forecasting techniques for heavy rainfall are summarized in terms of numerical weather prediction techniques and objective forecasting methods.Greatly aided by the rapid progress in meteorological observing technology and substantial improvement in electronic computing,studies of heavy rainfall in China have advanced to investigating the evolution of heavy-rain-producing storms and observational analysis of the cloud microphysical features.A deeper and more systematic understanding of the synoptic systems of importance to the production of heavy rainfall has also been developed.Operational forecast of heavy rainfall in China has changed from subjective weather event forecasts to a combination of both subjective and objective quantitative precipitation forecasts,and is now advancing toward probabilistic quantitative precipitation forecasts with the provision of forecast uncertainty information. | Yali LUO Jisong SUN Ying LI Rudi XIA Yu DU Shuai YANG Yuanchun ZHANG Jing CHEN Kan DAI Xueshun SHEN Haoming CHEN Feifan ZHOU Yimin LIU Shenming FU Mengwen WU Tiangui XIAO Yangruixue CHEN Huiqi LI Mingxin LI | 2020 | Journal of Meteorological Research2020，34，3： | 10 |
| 3 | Molecular characteristics of the omp A gene of serotype B Chlamydia trachomatis in Qinghai Tibetan primary school students显示文摘To study the molecular characteristics of Chlamydia trachomatis, the major outer membrane protein gene(omp A) of C. trachomatis from primary school students with trachoma residing in the Qinghai Tibetan area was sequenced and compared with the same serotype in Gen Bank. In Jianshetang Primary School and Galeng Central Primary School in the Galeng Tibetan Township of Qinghai Haidong Sala Autonomous County, scraped samples were collected from the upper tarsal conjunctiva and lower conjunctival sac of both eyes of 45 students with trachoma, stored at 4°C, and transported to Beijing Tongren Hospital by air within 24 h. The samples were screened for C. trachomatis by real-time PCR. The omp A gene from the C. trachomatis-positive samples was amplified by nested PCR. The serotype was confirmed by National Center for Biotechnology Information(NCBI) BLAST search and homology analysis. The entire omp A gene sequence was compared with the corresponding gene sequences of serotype B strains available in Gen Bank. Of the 45 students aged 6–13 years with trachoma, 26 C. trachomatis-positive students were identified by the initial real-time PCR screening(average age,(9.09±1.63) years; sex ratio, 1.0), accounting for 57.78%(26/45). The cycle threshold values for real-time PCR were 16.79–37.77. Half(13/26) of C. trachomatis-positive students had a bacterial copy number of >105. The compliance rate of the omp A gene sequences with the C. trachomatis serotype B strains in Gen Bank was up to 99%. Two novel genetic mutations were found when the omp A gene was compared with those of the 11 serotype B strains in Gen Bank. The two non-synonymous mutations were located at(i) position 271 in the second constant domain, an adenine(A) to guanine(G) substitution(ACT?GCT), changing the amino acid at position 91 from threonine to alanine(Thr?Ala) in all 26 strains; and(ii) position 887 in the fourth variable domain, a cytosine(C) to thymine(T) substitution(GCA?GTA), changing the amino acid at residue 296 from alanine to valine(Ala?Val) in four of the 26 strains. Six mutations were identified relative to ATCC VR-573. The strains could be divided into two gene clusters according to the mutation at nucleotide position 887: CQZ-1(China Qinghai Tibetan-1) and CQZ-2(China Qinghai Tibetan-2). We thus detected two novel serotype B mutant strains of C. trachomatis among study subjects with trachoma. | Xue Li Shaoya Zhang Qingfeng Liang Mei Wang Ailian Hu Xiuyuan Li Benshan Yang Mingxin Zhang Ningli Wang Xinxin Lu | 2016 | Science China(Life Sciences)2016，59，6： | 6 |
| 4 | Industrial Poverty Alleviation Model in Southwestern High-altitude Mountainous Areas of China——A Case Study of Industrial Poverty Alleviation of Xueshan Township in Luquan County of Yunnan Province through Planting Codonopsis pilosula显示文摘Due to the severe restrictions of natural conditions and ecological environment,high-altitude mountainous areas usually become the ' hard bones' in the battle against poverty. Xueshan Township,Luquan Yi and Miao Autonomous County of Yunnan Province,located in the alpine valley of Jinsha River,is a major township with wide and deep poverty,and the incidence of poverty is up to 45. 00%. In recent years,Xueshan Township has insisted on the battle against poverty,made effort to develop the Codonopsis pilosula industry,and successfully developed a road to poverty alleviation through C. pilosula industry,and formed a unique industrial poverty alleviation model by the end of 2018,the incidence of poverty dropped to 0. 74%. Based on field survey and interview,this paper analyzes and summarizes the specific practices,main results,practical experience and promotion and application measures of the poverty alleviation model of C. pilosula planting industry in Xueshan Township,in the hope of providing certain reference for the targeted poverty alleviation in similar areas in Yunnan Province and other provinces of China. | Zisheng YANG Renyi YANG Yanbo HE Chonghui LU En ZHU Yaohan PI Mingxin WU Ying XIONG | 2019 | Asian Agricultural Research2019，11，3： | 4 |
| 5 | Heavy ion and X-ray irradiation alter the cytoskeleton and cytomechanics of cortical neurons显示文摘Heavy ion beams with high linear energy transfer exhibit more beneficial physical and biological performance than conventional X-rays, thus improving the potential of this type of radiotherapy in the treatment of cancer. However, these two radiotherapy modalities both cause inevitable brain injury. The objective of this study was to evaluate the effects of heavy ion and X-ray irradiation on the cytoskeleton and cytomechanical properties of rat cortical neurons, as well as to determine the potential mechanism of neuronal injury after irradiation. Cortical neurons from 30 new-born mice were irradiated with heavy ion beams at a single dose of 2 Gy and X-rays at a single dose of 4 Gy; subsequent evaluation of their effects were carried out at 24 hours after irradiation. An immunofluorescence assay showed that after irradiation with both the heavy ion beam and X-rays, the number of primary neurons was significantly decreased, and there was evidence of apoptosis. Radiation-induced neuronal injury was more apparent after X-irradiation. Under atomic force microscopy, the neuronal membrane appeared rough and neuronal rigidity had increased. These cell changes were more apparent following exposure to X-rays. Our findings indicated that damage caused by heavy ion and X-ray irradiation resulted in the structural distortion and rearrangement of the cytoskeleton, and affected the cytomechanical properties of the cortical neurons. Moreover, this radiation injury to normal neurons was much severer after irradiation with X-rays than after heavy ion beam irradiation. | Yuting Du Jie Zhang Qian Zheng Mingxin Li Yang Liu Baoping Zhang Bin Liu Hong Zhang Guoying Miao | 2014 | Neural Regeneration Research2014，9，11： | 3 |
| 6 | Green Synthesis of Magnetic Adsorbent Using Groundwater Treatment Sludge for Tetracycline Adsorption显示文摘Groundwater treatment sludge is an industrial waste that is massively produced from groundwater treatment plants.Conventional methods for treatment of this sludge,such as discharge into deep wells or the sea,or disposal at landfills,are not environmentally sustainable.Here,we demonstrate an alternative strategy to recycle the sludge by preparing a magnetic maghemite adsorbent via a one-step hydrothermal method with NaOH solution as the only solvent.With this method,the weakly magnetized sludge,which contained 33.2%iron(Fe)and other impurities(e.g.,silicon(Si),aluminum(Al),and manganese(Mn)),was converted to magnetic adsorbent(MA)with the dissolution of Si/Al oxides(e.g.,quartz and albite)into the liquid fraction.At a NaOH concentration of 2 mol L^-1,approximately 18.1%of the ferrihydrite in the Fe oxides of the sludge was converted into 11.2%maghemite and 6.9%hematite after the hydrothermal treatment.MA2(i.e.,MA produced by a 2 mol L^-1 NaOH concentration)exhibited a good magnetic response of 8.2 emu g^-1(1 emu=10^-3 A m^2),and a desirable surface site concentration of 0.75 mmol g^-1.The synthesized MA2 was used to adsorb the cationic pollutant tetracycline(TC).The adsorption kinetics of TC onto MA2 fitted well with a pseudo-second-order model,and the adsorption isotherms complied well with the Langmuir model.The maximum adsorption capacity of MA2 for TC was 362.3 mg g^-1,and the main mechanism for TC adsorption was cationic exchange.This study is the first to demonstrate the preparation of an MA from recycled sludge without a reductant and/or exogenous Fe source.The prepared adsorbent can be used as a low-cost adsorbent with high capacity for TC sorption in the treatment of TC-containing wastewater. | Zhan Qu Yaqiong Wu Suiyi Zhu Yang Yu Mingxin Huo Leilei Zhang Jiakuan Yang Dejun Bian Yi Wang | 2019 | Engineering2019，5，5： | 2 |
| 7 | H5N6禽流感病毒的基因起源、进化和流行显示文摘2014~2016年间对我国16个省份活禽市场的监测,共鉴定了3174份流感阳性样品,分离并测序了1135份禽流感病毒,分属于31种亚型。 | yuhai bi quanjiao chen qianli wang jianjun chen tao jin gary wong chuansong quan jun liu jun wu renfu yin lihua zhao mingxin li zhuang ding rongrong zou wen xu hong li huijun wang kegong tian guanghua fu yu huang alexander shestopalov shoujun li bing xu hongjie yu tingrong luo lin lu xun xu yang luo 刘映霞 史卫峰 刘翟 高福 | 2017 | 科学新闻2017，0，4： | 2 |
| 8 | Multiple amino acid substitutions are involved in the adaptation of H9N2 avian influenza virus to mice显示文摘 | Rui Wu Hongbo Zhang Keli Yang Wangwang Liang Zhongliang Xiong Zewen Liu Xuehai Yang Huabin Shao Xinmin Zheng Mingxin Chen Diping Xu | 2009 | Veterinary Microbiology2009，，1： | 1 |
| 9 | Stationary Patterns of the Holling-Tanner Prey-predator Model with Diffusion and Cross-diffusion 显示文摘 | PENG Rui WANG Mingxin YANG Guoying | 2008 | Applied Mathematics and Computation2008，196，2： | 1 |
| 10 | Self-cleaning Anti-fouling TiO2/Poly(aryl ether sulfone) Composite Ultrafiltration Membranes显示文摘A series of novel TiO2/poly(aryl ether sulfone) ultrafiltration membranes with anti-fouling and self-cleaning properties was designed and prepared. First, anti-photocatalytic degraded fluorine contained polv(aryl ether sulfone) matrix(PAES-F) was synthesized. Then the composite membranes were prepared via TiO2 nanopartides and PAES-F polymer matrix by solution blending and non-solvent induced phase inversion methods. Further, separation efficiency, fouling behavior and self-cleaning property of the composite ultrafiltration(UF) membranes were investigated by dead-end filtration experiments using a polyacn lamide solution. The composite UF membranes exhibited outstanding self-cleaning efficiency and anti-photocatalytic degraded property after exposure to simulated sunliglit irradiation.The water flux recovery ratios(FRR)of the optimal composite UF membranes could reach 74.24%, which was attributable to photocatalytic degradation of the organic contaminant by TiO2. And the retention rates of the composite UF membranes could maintain over 97%, which indicated the excellent photocatalytic degradation resistance of the fluorine contained PAES-F matrix. The novel high perfomiance composite UF membranes have a broad application prospect in water treatment. | JIANG Hongchuan YANG Xia FENG Yong WANG Xinyu GENG Zhi HUO Mingxin | 2019 | Chemical Research in Chinese Universities2019，35，4： | 1 |
| 11 | Targeting the proinflammatory cytokine tumor necrosis factor-α to alleviatecardiopulmonary bypass-induced lung injury (Review)显示文摘 | Mingxin Gao Baodong Xie Chengxiong Gu Haitao Li Fan Zhang Yang Yu | 2015 | Molecular Medicine Reports2015，，4： | 1 |
| 12 | Leading-edge technologies in hydropower development in China显示文摘After the implementation of the Reform and Opening-up Policy for a period of 40 years, the exploitation of Chin a's hydropower resources developed significa ntly. Through vast amounts of scie ntific and tech no logical research and construction practices, China has accumulated abundant engineering experience with respect to the construction technologies required for 300-meter-high concrete arch dams, 200-meter-high roller-compacted concrete gravity dams, 200-meter-high concrete face rockfill dams, 250?meter?high earth core rockfill dams, large-flow discharge and energy dissipation, huge underground cavern group constructions, complicated foundation treatments for high earth and rockfill dams and high and steep slope reinforceme nts. These series of tech no logies have now reached an inter national leadi ng level. In the near future, these technical improvements will likely have broader application prospects and make greater contributions toward hydropower development both in China and across the world. | Zeyan Yang Chao Liu Mingxin Wu | 2019 | Global Energy Interconnection2019，2，3： | 1 |
| 13 | Effective enrichment of Zn from smelting wastewater via an integrated Fe coagulation and hematite precipitation method显示文摘Coagulation is commonly applied to treat Zn-bearing wastewater from smelting industries(smelting wastewater),and thus the Zn-bearing sludge was considerably produced,which should be solidified before safety disposal.Herein,we demonstrated a novel approach to recycle Zn effectively from smelting wastewater via an integrated Fe coagulation and hematite precipitation method.First,smelting wastewater was coagulated by adding ferric chloride to generate Fe/Zn-bearing sludge(sludge for short).Secondly,the sludge was dissolved to generate an acid solution containing 2.2 g/L of Zn and 39.2 g/L of Fe.Thirdly,the Fe/Zn-bearing solution was hydrothermally treated,and 89%of Fe was eliminated to highly purified hematite block,whereas the percentage of Zn lost was below 1.1%.Finally,the hematite precipitates were collected,and the supernatant was hydrothermally treated again with the addition of glucose.When the molar ratio of glucose to Fe in the supernatant was 1.5,over 99.5%of Fe was precipitated in hematite nanoparticles with a diameter of 10-100 nm,and the residual Fe was 21.5 mg/L.The loss of Zn was below 0.4%,and the residual Zn in the solution was 2169 mg/L,290 times of that in the smelting wastewater.The major mechanism for Fe removal was the hydrolysis of ferric nitrate into hematite,which was promoted by nitrate consumption in glucose oxidation.This paper is the first report of an environment-friendly method for enriching Zn without generating any waste. | Zhan Qu Ting Su Yu Chen Xue Lin Yang Yu Suiyi Zhu Xinfeng Xie Mingxin Huo | 2019 | Frontiers of Environmental Science & Engineering2019，13，6： | 1 |
| 14 | Stationary patterns of the holling-tanner prey-predator model with diffusion and cross-diffusion 显示文摘 | PENG Rui WANG Mingxin YANG Guoying | 2008 | Applied Mathematics and Computation2008，196，： | 1 |
| 15 | Turquoise from Hubei Province ,China显示文摘 | Qi Lijian Yan Weixuan Yang Mingxin | 1998 | The Journal of Gemmology1998，26，1： | 1 |
| 16 | Stationary patterns of the Holling-Tanner prey-predator model with diffusion and cross-diffnsion显示文摘 | Wang Mingxin Yang Guoying | 2008 | Appl Math Comput2008，196，2： | 1 |
| 17 | Node deployment of wireless sensor networks in long narrow area显示文摘 | SUN Xuguang HE Jingsha YANG Mingxin | 2012 | Applied Mechanics and Materials2012，128，4： | 1 |
| 18 | Novel Porous Polyethersulfone Beads as Matrix to Immobilize Comamonas testosteroni sp. bdq06 in Quinoline Biodegradation显示文摘 | GENG Zhi ZHU Suiyi YU Yang LU Ying LIN Rao GUO Shujun BIAN Dejun YANG Xia HUO Mingxin HUO Hongliang | 2015 | Chemical Research in Chinese Universities2015，31，4： | 1 |
| 19 | Turquoise from Hubei Province, China 显示文摘 | QI Lijian YAN Weixuan YANG Mingxin | 1998 | The Journal of Gemmology1998，26，1： | 1 |
| 20 | Comparing Polyethersulfone and Polyurethane-immobilized Cells of Comamonas testosteroni QYY in Treatment of an Accidental Dye Wastewater显示文摘 | GENG Zhi YU Yang ZHU Suiyi YU Hongbin LIU Jiancong BIAN Dejun YANG Xia HUO Hongliang HUO Mingxin | 2017 | Chemical Research in Chinese Universities2017，33，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员