3篇
您的检索式：作者名="Minna Cheng"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Prevalence and determinations of physical inactivity among public hospital employees in Shanghai, China: a cross-sectional study显示文摘这研究试图在上海在医院雇员之中探索物理迟钝的流行和决心,中国。19 ~ 68 年的 4612 个雇员的代表性的研究在 2011 从上海医院的不同的班通过成层的簇采样被进行。全部的物理活动根据全球物理活动问询表用新陈代谢的等价物被评估。在参加者之中, 38.5% , 32.3% ,和 64.6% 雇员在工作是不活跃的,变换,并且分别地花空闲时间。多达 41.8% 男人和 37.8% 女人(P = 0.012 ) 身体上是不活跃的。当年龄和教育水平被调整时,男医生和医药技术员在后勤比男工人显示出物理迟钝的一个更高的百分比(P = 0.001 ) 。在女性之中,雇员在二等车和三等车医院表演正在工作物理迟钝的一个更高的比例比在社区护理正在工作的那些集中。逻辑回归分析证明物理迟钝在工作与物理活动的紧张联系了的空闲时间的机会比率(ORs ) 为男人是 2.259, 2.897,和 4.266 (P P P < 0.001 ) 。医院雇员,特别地医生和医药技术员,在上海比另外的居民显示出物理迟钝的一个更高的比例。在工作的活动的时间和紧张并且变换与空闲时间活动被联系。 | Xinjian Li Minna Cheng Hao Zhang Ting Ke Yisheng Chen | 2015 | Frontiers of Medicine2015，9，1： | 0 |
| 2 | Optimization of Quantitative Real-time PCR System on Amplification of Beta-glucosidase Gene Os1bglu4显示文摘[Objective] This study aimed to establish a quantitative real-time PCR(qRT-PCR) system for detecting the expression of rice beta-glucosidase gene Os1bglu4. [Method] The PCR was conducted with SYBR Green I method, using the primers of reference gene actin or ubiquitin. [Result] Actin was more suitable to be the reference gene than ubiquitin. More accurate results were obtained when the 100 ng cDNA template was added at a large volume and a lower concentration.The primer concentration in the range from 0.2 to 0.8 μmol/L we set had no significant influence on the results, so, 0.4 μmol/L was selected as the optimal primer concentration in this study. The amplification efficiency was greatly reduced when the annealing temperature was set at 64 ℃, therefore, annealing temperature was set at 60 ℃. Compared with the reaction system of 25 μl, the fluorescence intensity was significantly lower but the CT value did not change greatly in 10 μl system.So, the 10 μl reaction system was selected, which significantly reduces the research costs for the detection of a large amount of samples in future study. | Rouyi CHEN Jiang CHENG Changxiang ZHENG Minna PAN Mariena KETUDAT-CAIRNS | 2014 | Agricultural Science & Technology2014，15，7： | 0 |
| 3 | Evaluation on Inducible Effect of pOp6 Promoter in Transgenic Rice显示文摘In order to analyze the Os1bglu4 phenotype,the inducible promoter of the transgenic rice which knock-down the Os1bglu4 expression was assessed.The result showed that 30μM dexamethasone(DEX)had the stronger induction effect than10μM DEX byβ-Glucuronidase(GUS)staining.qRT-PCR further verified the Os1bglu4 gene deletion.The effect of DEX and its solvent absolute ethanol on seed development was measured,and no significant effect was observed.The conclusion is that final concentration of DEX at 30μM is suitable for pOp6 promoter induction. | Rouyi CHEN Changxiang ZHENG Jiang CHENG Minna PAN Mariena KETUDAT-CAIRNS | 2014 | Agricultural Science & Technology2014，15，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员