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| 1 | The Performance of Whole Genome Amplification Methods and Next-Generation Sequencing for Pre-Implantation Genetic Diagnosis of Chromosomal Abnormalities显示文摘Reliable and accurate pre-implantation genetic diagnosis(PGD) of patient's embryos by next-generation sequencing(NGS) is dependent on efficient whole genome amplification(WGA) of a representative biopsy sample. However, the performance of the current state of the art WGA methods has not been evaluated for sequencing. Using low template DNA(15 pg) and single cells, we showed that the two PCR-based WGA systems Sure Plex and MALBAC are superior to the REPLI-g WGA multiple displacement amplification(MDA) system in terms of consistent and reproducible genome coverage and sequence bias across the 24 chromosomes, allowing better normalization of test to reference sequencing data. When copy number variation sequencing(CNV-Seq) was applied to single cell WGA products derived by either Sure Plex or MALBAC amplification, we showed that known disease CNVs in the range of 3e15 Mb could be reliably and accurately detected at the correct genomic positions. These findings indicate that our CNV-Seq pipeline incorporating either Sure Plex or MALBAC as the key initial WGA step is a powerful methodology for clinical PGD to identify euploid embryos in a patient's cohort for uterine transplantation. | Na Li Li Wang Hui Wang Minyue Ma Xiaohong Wang Yi Li Wenke Zhang Jianguang Zhang David S.Cram Yuanqing Yao | 2015 | Journal of Genetics and Genomics2015，42，4： | 13 |
| 2 | Fouling corrosion in aluminum heat exchangers显示文摘Fouling deposits on aluminum heat exchanger reduce the heat transfer efficiency and cause corrosion to the apparatus.This study focuses on the corrosive behavior of aluminum coupons covered with a layer of artificial fouling in a humid atmosphere by their weight loss,Tafel plots,electrochemical impedance spectroscopy(EIS),and scanning electron microscope(SEM)observations.The results reveal that chloride is one of the major elements found in the fouling which damages the passive film and initiates corrosion.The galvanic corrosion between the metal and the adjacent carbon particles accelerates the corrosive process.Furthermore,the black carbon favors the moisture uptake,and gives the dissolved oxygen greater chance to migrate through the fouling layer and form a continuous diffusive path.The corrosion rate decreasing over time is conformed to electrochemistry measurements and can be verified by Faraday’s law.The EIS results indicate that the mechanism of corrosion can be interpreted by the pitting corrosion evolution mechanism,and that pitting was observed on the coupons by SEM after corrosive exposure. | Su Jingxin Ma Minyu Wang Tianjing Guo Xiaomei Hou Liguo Wang Zhiping | 2015 | Chinese Journal of Aeronautics2015，28，3： | 5 |
| 3 | A PGD Pregnancy Achieved by Embryo Copy Number Variation Sequencing with Confirmation by Non-Invasive Prenatal Diagnosis显示文摘Aneuploidy rates in embryos produced by assisted reproductive technologies commonly exceed 50%,particularly in couples where the woman is of advanced maternal age or has experienced repeated implantation failure(Harper et al.,2012).Pre-implantation genetic diagnosis(PGD)conducted at many fertility clinics worldwide is now | Hui Wang Li Wang Minyue Ma Zhuo Song Jianguang Zhang Genming Xu Junmei Fan Na Li David S.Cram Yuanqing Yao | 2014 | Journal of Genetics and Genomics2014，41，8： | 3 |
| 4 | Generation of patient-specific pluripotent stem cells and directed differentiation of embryonic stem cells for regenerative medicine显示文摘Embryonic stem(ES) cells are pluripotent cells that can give rise to derivatives of all three embryonic germ layers. Due to its characteristics, the patient-specific ES cells are of great potential for transplantation therapies. Several strategies can reprogramme somatic cells back to pluripotent stem cells: nuclear transfer, fusion with ES cells, treatment with cell extract and induction by specific factors. Considering the future clinical use, the differentiation from ES to neurons, cardiomyocytes and many other types of cells currently provide basic cognition and experience to regenerative medicine. This article will review two courses, the reprogramming of differentiated cells and the differentiation of ES cells to specific cell types. | Minyue Ma Jiahao Sha Zuomin Zhou Qi Zhou Qingzhang Li | 2008 | Journal of Nanjing Medical University2008，22，3： | 1 |
| 5 | 艾灸标准化国际高端论坛在中国北京隆重召开(英文)显示文摘During 23 to 24 January 2015,the Moxibustion International Standardization Forum,which is co-hosted by Beijing Traditional Chinese Medicine International Exchange&Co-operation Centre and Beijing University of Chinese Medicine is solemnly held in Beijing,China.More than 40 experts from a range of countries and regions attended the meeting,including China,Japan,South Korea,Australia,Italy,Brazil,Hong Kong,and other countries | MA Liangxiao LIN Minyu HA Lue | 2015 | World Journal of Acupuncture-Moxibustion2015，25，1： | 0 |
| 6 | Single-cell Sequencing Reveals Clearance of Blastula Chromosomal Mosaicism in In Vitro Fertilization Babies显示文摘Although chromosomal mosaic embryos detected by trophectoderm(TE)biopsy offer healthy embryos available for transfer,high-resolution postnatal karyotyping and chromosome testing of the transferred embryos are insufficient.Here,we applied single-cell multi-omics sequencing for seven infants with blastula chromosomal mosaicism detected by TE biopsy.The chromosome ploidy was examined by single-cell genome analysis,with the cellular identity being identified by single-cell transcriptome analysis.A total of 1616 peripheral leukocytes from seven infants with embryonic chromosomal mosaicism and three control ones with euploid TE biopsy were analyzed.A small number of blood cells showed copy number alterations(CNAs)on seemingly random locations at a frequency of 0%-2.5%per infant.However,none of the cells showed CNAs that were the same as those of the corresponding TE biopsies.The blastula chromosomal mosaicism may be fully self-corrected,probably through the selective loss of the aneuploid cells during development,and the transferred embryos can be born as euploid infants without mosaic CNAs corresponding to the TE biopsies.The results provide a new reference for the evaluations of transferring chromosomal mosaic embryos in certain situations. | Yuan Gao Jinning Zhang Zhenyu Liu Shuyue Qi Xinmeng Guo Hui Wang Yanfei Cheng Shuang Tian Minyue Ma Hongmei Peng Lu Wen Fuchou Tang Yuanqing Yao | 2022 | Genomics, Proteomics & Bioinformatics2022，20，6： | 0 |
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