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| 1 | Ythdc2 is an N^6-methyladenosine binding protein that regulates mammalian spermatogenesis显示文摘N 6-methyladenosine (m 6 一) 是在真核细胞的 mRNA 的最普通的内部修正。它动态地被安装并且搬迁,并且充当 mRNA 新陈代谢,包括干细胞 pluripotency 的调整生物过程,房间区别,和精力动态平衡的新层。m 6 A 被选择有约束力的蛋白质认出;YTHDF1 和 YTHDF3 在音乐会工作影响 m 6包含 A 的 mRNAs, YTHDF2 帮助 mRNA 腐烂,和 YTHDC1 影响它的目标的原子处理。YTHDC2 的生物功能, YTH 蛋白质家庭,的最后的成员仍然保持未知。我们报导 YTHDC2 有选择地绑 m 6 在它的一致主题的 A。YTHDC2 提高它的目标的翻译效率并且也减少他们的 mRNA 丰富。Ythdc2 猛烈老鼠是不肥沃的;男性们让显著地更小的睾丸和女性同窝出生的人与那些相比有显著地更小的卵巢。Ythdc2 猛烈老鼠的细菌房间不经过 zygotene 阶段发展,因此,当成熟分裂开始, Ythdc2 是在睾丸的 upregulated。因此, YTHDC2 是 m 6 在精子发生期间起关键作用的 A 绑定蛋白质。 | Phillip J Hsu Yunfei Zhu Honghui Ma Yueshuai Guo Xiaodan Shi Yuanyuan Liu Meijie Qi Zhike Lu Hailing Shi Jianying Wang Yiwei Cheng Guanzheng Luo Qing Dai Mingxi Liu Xuejiang Guo Jiahao Sha Bin Shen Chuan He | 2017 | Cell Research2017，27，9： | 96 |
| 2 | Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis显示文摘 | Yao Chen Yuxuan Zheng Yun Gao Zhen Lin Suming Yang Tongtong Wang Qiu Wang Nannan Xie Rong Hua Mingxi Liu Jiahao Sha Michael D. Griswold Jinsong Li Fuchou Tang Ming-Han Tong | 2018 | Cell Research2018，28，9： | 26 |
| 3 | Generation of a precise Oct4-hrGFP knockin cynomolgus monkey model via CRISPR/Cas9-assisted homologous recombination显示文摘 | Yiqiang Cui Yuyu Niu Jiankui Zhou Yongchang Chen Yiwei Cheng Shangang Li Zongyong Ai Chu Chu Hong Wang Bo Zheng Xuejin Chen Jiahao Sha Xuejiang Guo Xingxu Huang Weizhi Ji | 2018 | Cell Research2018，28，3： | 13 |
| 4 | Germline acquisition of Cas9/RNA-mediated gene modifications in monkeys显示文摘 | Yongchang Chen Yiqiang Cui Bin Shen Yuyu Niu Xiaoyang Zhao Lei Wang Jianying Wang Wei Li Qi Zhou Weizhi Ji Jiahao Sha Xingxu Huang | 2015 | Cell Research2015，25，2： | 8 |
| 5 | Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility显示文摘Proteomic 技术在最近的年里经历了重要开发,它在蛋白质研究导致了广泛的进展。当前, proteomic 途径被用于许多科学区域,包括基本研究,各种各样的疾病和恶性肿瘤诊断, biomarker 发现和另外的治疗学的应用。另外,检验繁殖生物学和药的驾驶 proteomics 的研究文章正在变得逐渐地普通。为这个领域的关键挑战是从识别蛋白质的表搬到关于蛋白质功能获得生物信息。现在的文章考察与精子发生有关的可得到的科学文学。另外,这研究使用二维的电气泳动团 spectrometry (2DEMS ) ,构造一系列 proteome 的液体层析(LC ) MS 介绍描述精子发生。蛋白质的这大规模鉴定为阐明提供一个富有的资源位于男富饶和不孕下面的机制。 | Xiaoyan Huang Jiahao Sha | 2011 | Asian Journal of Andrology2011，13，1： | 7 |
| 6 | Generation of fertile offspring from Kitw/Kitwv mice through differentiation of gene corrected nuclear transfer embryonic stem cells显示文摘 | Yan Yuan Quan Zhou Haifeng Wan Bin Shen Xuepeng Wang Mei Wang Chunjing Feng Mingming Xie Tiantian Gu Tao Zhou Rui Fu Xingxu Huang Qi Zhou Jiahao Sha Xiao-Yang Zhao | 2015 | Cell Research2015，25，7： | 7 |
| 7 | In vitro testicular organogenesis from human fetal gonads produces fertilization-competent spermatids显示文摘Unlike most organs that mature during the fetal period,the male reproductive system reaches maturity only at puberty with the commencement of spermatogenesis.Robust modelling of human testicular organogenesis in vitro would facilitate research into mechanisms of and factors affecting human spermatogenic failure and male fertility preservation in prepubertal tumor patients.Here,we report successful recapitulation of human testicular organogenesis in vitro from fetal gonadal ridge.Our model displayed the formation of mature seminiferous epithelium and self-renewing spermatogonia.Remarkably,in vitro-derived haploid spermatids have undergone meiotic recombination,and showed increased genetic diversity as indicated by genetic analysis.Moreover,these spermatids were able to fertilize oocytes and support subsequent blastocyst formation.The in vitro testicular organogenesis system described here will play an important role in elucidating the regulation of human testis development and maintaining male fertility in prepubertal cancer patients. | Yan Yuan Laihua Li Qing Cheng Feiyang Diao Qiao Zeng Xiaoyu Yang Yibo Wu Hao Zhang Mingqian Huang Junqing Chen Quan Zhou Yunfei Zhu Rong Hua Jianyu Tian Xin Wang Zuomin Zhou Jie Hao Jinjin Yu Dong Hua Jiayin Liu Xuejiang Guo Qi Zhou Jiahao Sha | 2020 | Cell Research2020，30，3： | 6 |
| 8 | DDX3X regulates cell survival and cell cycle during mouse early embryonic development显示文摘DDX3X is a highly conserved DEAD-box RNA helicase that participates in RNA transcription,RNA splicing,and mRNA transport,translation,and nucleo-cytoplasmic transport.It is highly expressed in metaphaseⅡ(MⅡ)oocytes and is the predominant DDX3 variant in the ovary and embryo.However,whether it is important in mouse early embryo development remains unknown.In this study,we investigated the function of DDX3X in early embryogenesis by cytoplasmic microinjection with its siRNA in zygotes or single blastomeres of 2-cell embryos.Our results showed that knockdown of Ddx3x in zygote cytoplasm led to dramatically diminished blastocyst formation,reduced cell numbers,and an increase in the number of apoptotic cells in blastocysts.Meanwhile,there was an accumulation of p53 in RNAi blastocysts.In addition,the ratio of cell cycle arrest during 2-cell to 4-cell transition increased following microinjection of Ddx3x siRNA into single blastomeres of 2-cell embryos compared with control.These results suggest that Ddx3x is an essential gene associated with cell survival and cell cycle control in mouse early embryos,and thus plays key roles in normal embryo development. | Qian Li Pan Zhang Chao Zhang Ying Wang Ru Wan Ye Yang Xuejiang Guo Ran Huo Min Lin Zuomin Zhou Jiahao Sha | 2014 | The Journal of Biomedical Research2014，28，4： | 4 |
| 9 | Flotillin-2 is an acrosome-related protein involved in mouse spermiogenesis显示文摘Spermatogenesis is a complex process of terminal differentiation by which mature sperms are generated,and it can be divided into three phases:mitosis,meiosis and spermiogenesis.In a previous study,we established a series of proteomic profiles for spermatogenesis to understand the regulation of male fertility and infertility.Here,we further investigated the localization and the role of flotillin-2 in spermiogenesis.Flotillin-2 expression was investigated in the testis of male CD1 mice at various developmental stages of spermatogenesis by using Western blotting,immunohistochemistry and immunofluorescence.Flotillin-2 was knocked down in vivo in three-week-old male mice using intratesticular injection of small inhibitory RNA(siRNA),and sperm abnormalities were assessed three weeks later.Flotillin-2 was expressed at high levels in male germ cells during spermatogenesis.Flotillin-2 immunoreactivity was observed in pachytene spermatocytes as a strong dot-shaped signal and in round spermatids as a sickle-shaped distribution ahead of the acrosome.Immunofluorescence confirmed flotillin-2 was localized in front of the acrosome in round spermatids,indicating that flotillin-2 was localized to the Golgi apparatus.Knockdown of flotillin-2 in vivo led to a significant increase in head sperm abnormalities isolated from the cauda epididymis,compared with control siRNA-injected testes.This study indicates that flotillin-2 is a novel Golgi-related protein involved in sperm acrosome biogenesis. | Yibo Wu Xin Chen Shuai Wang Min Jiang Bo Zheng Quan Zhou Ye Bi Zuomin Zhou Xiaoyan Huang Jiahao Sha | 2012 | The Journal of Biomedical Research2012，26，4： | 3 |
| 10 | PNAS:科学家发现新的精子运动调节器显示文摘南京医科大学生殖医学国家重点实验室、基础医学院组织胚胎学系刘明兮课题组与贝勒医学院MartinM.Matzuk课题组、大阪大学MasahitoIkawa课题组等共同解析了一个精子运动调节基因TCTE1,结果发表于《美国科学院院刊》(PNAs)。 | Castaneda, Julio M. Hua, Rong Miyata, Haruhiko Oji, Asami Guo, Yueshuai Cheng, Yiwei Zhou, Tao Guo, Xuejiang Cui, Yiqiang Shen, Bin Wang, Zibin Hu, Zhibin Zhou, Zuomin Sha, Jiahao Prunskaite-Hyyrylainen, Renata Yu, Zhifeng Ramirez-Solis, Ramiro Ikawa, Masahito Matzuk, Martin M. Liu, Mingxi | 2017 | 现代生物医学进展2017，17，27： | 3 |
| 11 | Association of assisted reproductive technology, germline de novo mutations and congenital heart defects in a prospective birth cohort study显示文摘Emerging evidence suggests that children conceived through assisted reproductive technology(ART)have a higher risk of congenital heart defects(CHDs)even when there is no family history.De novo mutation(DNM)is a well-known cause of sporadic congenital diseases;however,whether ART procedures increase the number of germline DNM(gDNM)has not yet been well studied.Here,we performed whole-genome sequencing of 1137 individuals from 160 families conceived through ART and 205 families conceived spontaneously.Children conceived via ART carried 4.59 more gDNMs than children conceived spontaneously,including 332 paternal and 1.26 maternal DNMs,after correcting for parental age at conception,cigarette smoking,alcohol drinking,and exercise behaviors.Paternal DNMs in offspring conceived via ART are characterized by C>T substitutions at CpG sites,which potentially affect protein-coding genes and are significantly associated with the increased risk of CHD.In addition,the accumulation of non-coding functional mutations was independently associated with CHD and 87.9% of the mutations were originated from the father.Among ART offspring,infertility of the father was associated with elevated paternal DNMs;usage of both recombinant and urinary follicle-stimulating hormone and high-dosage human chorionic gonadotropin trigger was associated with an increase of maternal DNMs.In sum,the increased gDNMs in offspring conceived by ART were primarily originated from fathers,indicating that ART itself may not be a major reason for the accumulation of gDNMs.Our findings emphasize the importance of evaluating the germline status of the fathers in families with the use of ART. | Cheng Wang Hong Lv Xiufeng Ling Hong Li Feiyang Diao Juncheng Dai Jiangbo Du Ting Chen Qi Xi Yang Zhao Kun Zhou Bo Xu Xiumei Han Xiaoyu Liu Meijuan Peng Congcong Chen Shiyao Tao Lei Huang Cong Liu Mingyang Wen Yangqian Jiang Tao Jiang Chuncheng Lu Wei Wu Di Wu Minjian Chen Yuan Lin Xuejiang Guo Ran Huo Jiayin Liu Hongxia Ma Guangfu Jin Yankai Xia Jiahao Sha Hongbing Shen Zhibin Hu | 2021 | Cell Research2021，31，8： | 2 |
| 12 | An essential role for PNLDC1 in piRNA 3' end trimming and male fertility in mice显示文摘 | Yue Zhang Rui Guo Yiqiang Cui Zhiping Zhu Yingwen Zhang Hao Wu Bo Zheng Qiuling Yue Shun Bai Wentao Zeng Xuejiang Guo Zuomin Zhou Bin Shen Ke Zheng Mingxi Liu Lan Ye Jiahao Sha | 2017 | Cell Research2017，27，11： | 2 |
| 13 | The expression and localization of a novel protein phosphatase inhibitor 2810408A11Rik in mouse testis and sperm显示文摘This study investigated the expression and distribution of 2810408A11Rik in mouse testis and sperm,and explored its role in spermatogenesis and sperm function.The expression levels of 2810408A11Rik mRNA in multiple tissue samples were analyzed using bioinformatic resources and RT-PCR technique.A specific rabbit polyclonal antibody was prepared by prokaryotic expression of 2810408A11Rik recombinant protein and utilized for animal immunization.Western blotting,immunohistochemistry and immunofluorescence were used to detect the expression and distribution of 2810408A11Rik.The results of the bioinformatic analysis and RT-PCR showed that 2810408A11Rik mRNA was specifically expressed in mouse testis,and 2810408A11Rik protein included a protein phosphatase inhibitor domain.Western blotting assays,immunohistochemistry and immunofluorescence confirmed the expression of 2810408A11Rik protein in mouse testis,especially in post-meiosis round and long spermatids,and that it is localized in the acrosome and the post-nucleus area of sperm.Our findings suggest that 2810408A11Rik may play an important role in spermatogenesis,sperm capacitation and fertilization. | Ye Bi Mingxi Liu Wenjiao Tu Yibo Wu Xuejiang Guo Zuomin Zhou Jiahao Sha | 2012 | The Journal of Biomedical Research2012，26，2： | 1 |
| 14 | The toxic effects of microeystin-LR on the reproductive system of male rats in vivo and in vitro 显示文摘 | Yan Li Jie Sheng Jiahao Sha XiaoDong Han | 2008 | Reproductive Toxicology2008，26，： | 1 |
| 15 | RNA Guided Genome Editing in Mouse Germ-Line Stem Cells显示文摘Spermatogonial stem cells(SSCs)reside on the basement membrane of the seminiferous tubules in mammalian testes(Nagano et al.,1998).After isolation and purification of SSCs from mouse testis,SSCs can be cultured in vitro to derive germ-line stem cells(GSCs)which have the ability of proliferation over 2 years(Kanatsu-Shinohara et al.,2003;KanatsuShinohara and Shinohara,2007).GSCs can be localized in the seminiferous tubules to re-establish the spermatogenesis | Xuepeng Wang Yan Yuan Quan Zhou Haifeng Wan Mei Wang Qi Zhou Xiao-Yang Zhao Jiahao Sha | 2014 | Journal of Genetics and Genomics2014，41，7： | 1 |
| 16 | A spermatogenesis-related gene expression profile in human spermatozoa and its potential clinical applications显示文摘 | Hui Wang Zuomin Zhou Min Xu Jianmin Li Junhua Xiao Zhi-Yang Xu Jiahao Sha | 2004 | Journal of Molecular Medicine2004，，5： | 1 |
| 17 | Establishment of a proteomic profile associated with gonocyte and spermatogonial stem cell maturation and differentiation in neonatal mice显示文摘 | Bo Zheng Quan Zhou Yueshuai Guo Binbin Shao Tao Zhou Lei Wang Zuomin Zhou Jiahao Sha Xuejiang Guo Xiaoyan Huang | 2014 | Proteomics (-)2014，，2： | 1 |
| 18 | A susceptibility locus rs7099208 is associated with non-obstructive azoospermia via reduction in the expression of FAM160B1显示文摘Non-obstructive azoospermia(NOA) is a severe defect in male reproductive health that occurs in 1%of adult men.In a previous study,we identified that rs7099208 is located within the last intron of FAM160B1 at10q25.3.In this study,we analysed expression Quantitative Trait Loci(eQTL) of FAM160B1,ABLIM1 and TRUB1,the three genes surrounding rs7099208.Only the expression level of FAM160B1 was reduced for the homozygous alternate genotype(GG) of rs7099208,but not for the homozygous reference or heterozygous genotypes.FAM160B1 is predominantly expressed in human testes,where it is found in spermatocytes and round spermatids.From 17 patients with NOA and five with obstructive azoospermia(OA),immunohistochemistry revealed that expression of FAM160B1 is reduced,or undetectable in NOA patients,but not in OA cases or normal men.We conclude that rs7099208 is associated with NOA via a reduction in the expression of FAM160B1. | Yan Zhang Jing Qian Minghui Wu Mingxi Liu Kai Zhang Yuan Lin Xuejiang Guo Zuomin Zhou Zhibin Hu Jiahao Sha | 2015 | The Journal of Biomedical Research2015，29，6： | 1 |
| 19 | Generation of patient-specific pluripotent stem cells and directed differentiation of embryonic stem cells for regenerative medicine显示文摘Embryonic stem(ES) cells are pluripotent cells that can give rise to derivatives of all three embryonic germ layers. Due to its characteristics, the patient-specific ES cells are of great potential for transplantation therapies. Several strategies can reprogramme somatic cells back to pluripotent stem cells: nuclear transfer, fusion with ES cells, treatment with cell extract and induction by specific factors. Considering the future clinical use, the differentiation from ES to neurons, cardiomyocytes and many other types of cells currently provide basic cognition and experience to regenerative medicine. This article will review two courses, the reprogramming of differentiated cells and the differentiation of ES cells to specific cell types. | Minyue Ma Jiahao Sha Zuomin Zhou Qi Zhou Qingzhang Li | 2008 | Journal of Nanjing Medical University2008，22，3： | 1 |
| 20 | Genetic Modification and Screening in Rat Using Haploid Embryonic Stem Cells显示文摘 | Wei Li Xin Li Tianda Li Ming-Gui Jiang Haifeng Wan Guan-Zheng Luo Chunjing Feng Xiaolong Cui Fei Teng Yan Yuan Quan Zhou Qi Gu Ling Shuai Jiahao Sha Yamei Xiao Liu Wang Zhonghua Liu Xiu-Jie Wang Xiao-Yang Zhao Qi Zhou | 2013 | Cell Stem Cell2013，，： | 1 |
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