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| 1 | 临床致病性不动杆菌的分子生物学鉴定显示文摘目的对临床致病性不动杆菌进行种属鉴定,并比较3种方法(44℃生长试验、特异性引物PCR扩增和16SrRNA测序)对鲍曼不动杆菌鉴定的特异性、准确性及应用价值。方法收集临床致病不动杆菌56株,首先接种在胰蛋白胨大豆肉汤中44℃进行培养,观察是否可以生长;其次,提取56株不动杆菌基因组DNA,用两组不同的特异性引物进行PCR扩增鉴定;最后,用通用引物对56株不动杆菌的16SrRNA进行测序鉴定。结果 56株不动杆菌中有17株可在44℃生长。经特异性引物PCR扩增鉴定,发现8株鲍曼不动杆菌和3株13TU型不动杆菌均可在44℃中较早较快生长。金标准16SrRNA测序确定56株不动杆菌中含有9个种,其中鲍曼不动杆菌和13TU型不动杆菌的鉴定结果与特异性引物PCR扩增法相同。结论应用分子生物学方法鉴定鲍曼不动杆菌具有准确、高效且可重复性高的优点,尤其是特异性PCR扩增法,可作为较难诊断的鲍曼不动杆菌的首选鉴定方法。 | 韩蕾 张旭燕 韩少山 MCCARRON Andrew MOORE John E MILLAR Cherie B 徐纪茹 | 2012 | 西安交通大学学报(医学版)2012，33，5： | 4 |
| 2 | 卒中一级预防指南美国心脏协会/美国卒中协会对医疗专业人员的声明显示文摘这份更新版指南旨在为既往无卒中或短暂性脑缺血发作史的人群提供适时的综合性卒中预防循证建议,包括危险因素控制、头颈部动脉粥样硬化性疾病的干预方法以及预防血栓形成和血栓栓塞性卒中的抗栓治疗。此外,本指南还提供了针对遗传和药理学检测以及在其他特殊情况下(包括镰状细胞疾病和卵圆孔未闭)进行卒中预防的建议。 | James F. Meschia Cheryl Bushnell Bernadette Boden-Albala Lynne T. Braun Dawn M. Bravata Seemant Chaturvedi Mark A. Creager Robert H. Eckel Mitchell S.V. Elkind Myriam Fornage Larry B Goldstein Steven M. Greenberg Susanna E. Horvath Costantino Iadecola Edward C. Jauch Wesley S. Moore John A. Wilson 李迪 高一鹭 陈政弘 霍晓川 孙海欣 孙冬玲 茹小娟 江滨 | 2015 | 国际脑血管病杂志2015，23，6： | 4 |
| 3 | A survey of FLS2 genes from multiple citrus species identifies candidates for enhancing disease resistance to Xanthomonas citri ssp. citri.显示文摘Pathogen-associated molecular patterns(PAMPs)-triggered immunity(PTI)is an important component of plant innate immunity.In a previous study,we showed that the PAMP flg22 from Xanthomonas citri ssp.citri(Xflg22),the causal agent of citrus canker,induced PTI in citrus,which correlated with the observed levels of canker resistance.Here,we identified and sequenced two bacterial flagellin/flg22 receptors(FLS2-1 and FLS2-2)from‘Duncan’grapefruit(Citrus paradisi,CpFLS2-1 and CpFLS2-2)and‘Sun Chu Sha’mandarin(C.reticulata,CrFLS2-1 and CrFLS2-2).We were able to isolate only one FLS2 from‘Nagami’kumquat(Fortunella margarita,FmFLS2-1)and gene flanking sequences suggest a rearrangement event that resulted in the deletion of FLS2-2 from the genome.Phylogenetic analysis,gene structure and presence of critical amino acid domains all indicate we identified the true FLS2 genes in citrus.FLS2-2 was more transcriptionally responsive to Xflg22 than FLS2-1,with induced expression levels higher in canker-resistant citrus than in susceptible ones.Interestingly,‘Nagami’kumquat showed the highest FLS2-1 steady-state expression levels,although it was not induced by Xflg22.We selected FmFLS2-1,CrFLS2-2 and CpFLS2-2 to further evaluate their capacity to enhance bacterial resistance using Agrobacterium-mediated transient expression assays.Both FmFLS2-1 and CrFLS2-2,the two proteins from canker-resistant species,conferred stronger Xflg22 responses and reduced canker symptoms in leaves of the susceptible grapefruit genotype.These two citrus genes will be useful resources to enhance PTI and achieve resistance against canker and possibly other bacterial pathogens in susceptible citrus types. | Qingchun Shi Vicente J Febres Jeffrey B Jones Gloria A Moore | 2016 | Horticulture Research2016，3，1： | 3 |
| 4 | Mobilization of Endothelial and Hematopoietic Stem and Progenitor Cells by Adenovector Mediated Elevation of Serum Levels of SDF-1,VEGF and Angiopoietin-l显示文摘 | Moore MA Hattori K Heissig B | 2001 | Ann N Y Acad Sci2001，938，： | 2 |
| 5 | High prevalence of antifungal resistance in Candida spp from patients with AIDS 显示文摘 | Law D Moore C B Wardle H M | 1994 | J Antimicrob Chemother1994，34，5： | 2 |
| 6 | Corrosion protection of magnesium by electroactive polypyrrole/paint coatings显示文摘 | Truong V T Lai P K Moore B T | 2000 | Synthetic Metals2000，111，： | 2 |
| 7 | Abundance and partitioning of OH in a hlgh-pressure magmatic system: Megacrysts from the Monastery kimberlite, South Africa显示文摘 | Bell D B Rossman G R Moore R O | 2004 | J Petrol2004，45，： | 1 |
| 8 | Complete genomes of two clinical Staphylococcus aureus strains:evidence for the rapid evolution of virulence and drug resistance显示文摘 | Holden MT Feil EJ Lindsay JA Peacock SJ Day NP Enright MC Foster TJ Moore CE Hurst L Atkin R Barron A Bason N Bentley SD Chillingworth C Chillingworth T Churcher C Clark L Corton C Cronin A Doggett J Dowd L Feltwell T Hance Z Harris B Hauser H Holroyd S Jagels K James KD Lennard N Line A Mayes R Moule S Mungall K Ormond D Quail MA Rabbinowitsch E Rutherford K Sanders M Sharp S Simmonds M Stevens K Whitehead S Barrell BG Spratt BG Parkhill J | 2004 | Proc Natl Acad Sci USA2004，101，26： | 1 |
| 9 | The phytoestrogen coumestrol is a naturally occurring antagonist of the human pregnane X receptor显示文摘 | WANG H W LI H MOORE L B | 2008 | Mol Endocrinol2008，22，4： | 1 |
| 10 | Experimental behavior of a steel structure under natural fire 显示文摘 | Wald F Sim?es da Silva L Moore D B | 2006 | Fire Safety Journal2006，41，7： | 1 |
| 11 | Recent advances in stream and river temperature research显示文摘 | WEBB B W HANNAH D M MOORE R D | 2008 | Hydrological Processes2008，22，7： | 1 |
| 12 | Erythropoietin (EPO) in acute kidney injury 显示文摘 | Elizabeth Moore R B | 2011 | Annals of Intensive Care2011，1，3： | 1 |
| 13 | Comparison of API 20 C with molecular identification of Candida spp isolated from bloodstream infections显示文摘 | J Xu B C Millar J E Moore | | J Clin Pathol0，55，： | 1 |
| 14 | Lower gastrointestinal bleeding显示文摘 | AM 3rd MOORE B A LONGOW E | 1997 | Disease of Colon & Rectum1997，40，7： | 1 |
| 15 | Recent advances in stream and river temperature research显示文摘 | Webb B W Hannah D M Moore R D | 2008 | Hydrological Processes2008，22，： | 1 |
| 16 | Induction of macro- phage nitric oxide production by Gram-negative flagellin involves signaling via heteromeric Toll-like receptor 5/Toll-like receptor 4 complexes显示文摘 | Mizel S B Honko A N Moors M A | 2003 | J Immunol2003，170，12： | 1 |
| 17 | Invasive Pneumococcal Disease in Children 5 Years After Conjugate Vaccine Introduction - Eight States, 1998-2005显示文摘 | Reingold A Hadler J Farley M M Harrison L Lynfield R Lexau C Bennett N Thomas A Craig A S Smith P J Beall B Whitney C G Moore M Pilishvili T | 2008 | MMWR Morbidity and Mortality Weekly Report2008，，6： | 1 |
| 18 | The behaviour of full-scale steel framed buildings subjected to compartment fires显示文摘 | Bailey C G Lennon T Moore D B | 1999 | The Structural Engineer1999，77，8： | 1 |
| 19 | Synthesis of brightly PEGylated luminescentmagnetic upconversion nanophosphors for deep tissue and dual MRI imaging 显示文摘 | Chen H Qi B Moore T | 2014 | Small2014，10，1： | 1 |
| 20 | Murine models of puhnonary fi- brosis显示文摘 | Moore B B Hogaboam C M | 2008 | Am J Physiol Lung Cell Mol Physiol2008，294，2： | 1 |
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