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| 1 | 运用PTEN和ERG免疫染色区分针吸活检组织中前列腺导管内癌以及高级别PIN显示文摘前列腺导管内癌和高级别上皮内瘤变(PIN)在治疗方案上有着显著的区别,但二者的针吸活检组织很难区分。在前列腺根治标本中,作者发现运用PTEN和ERG的免疫组化染色可以解决这一难题。作者在实验中进一步验证在针吸标本中这些标志物是否有用。单独或组合的免疫标记法运用于形态学明确的导管内癌、PIN、交界性导管内增生的活检,后者缺乏类似于导管内癌的形态学准则而比PIN更令人担忧。 | Morais C L Han J S Gordetsky J 徐磊 孙咏梅 | 2015 | 临床与实验病理学杂志2015，31，4： | 11 |
| 2 | Bartter's syndrome:clinical findings,genetic causes and therapeutic approach显示文摘Backgound Bartter's syndrome(BS)is a rare group of salt losing tubulopathies due to the impairment of transport mecha-nisms at the thick ascending limb of the Henle's loop.Data sources Literature reviews and original research articles were collected from database,including PubMed and Scopus.Results According to the time of onset and symptoms,BS can be classified into antenatal and classic BS.Molecular studies have identified different subtypes of BS.BS types Ⅰ,Ⅱ and Ⅲ are caused by mutations on genes encoding the luminal Na^(+)-K^(+)-2Cl^(-) co-transporter,the luminal K+ channel ROMK,and the basolateral chloride channel ClC-Kb(CLCNKB),respectively.Loss-of-function mutations of Barttin CLCNK type accessory beta subunit cause BS type Ⅳa.Simultaneous mutations of CLCNKB and CLCNKA cause BS type Ⅳb.BS type Ⅴ consists in a novel transient form characterized by antenatal presentation due to mutations in the MAGE family member D2.Severe gain-of-function mutations of the extracellular calcium sensing receptor gene can result in an autosomal dominant condition of BS.Main clinical and biochemical alterations in BS include polyuria,dehydration,hypokalemia,hypochloremic metabolic alka-losis,hyperreninemia,high levels of prostaglandins,normal or low blood pressure,hypercalciuria and failure to thrive.Treatment focuses mainly at correcting dehydration and electrolyte disturbances and in measures to reduce polyuria,including the use of nonsteroidal anti-inflammatory medications to control excessive renal prostaglandin E2 production.Conclusions Early diagnosis and treatment of BS may prevent long-term consequences such as growth failure,nephrocal-cinosis and end-stage renal disease. | Flavia Cristina Carvalho Mrad Sílvia Bouissou Morais Soares Luiz Alberto Wanderley de Menezes Silva Pedro Versiani dos Anjos Menezes Ana Cristina Simoes-e-Silva | 2021 | World Journal of Pediatrics2021，17，1： | 10 |
| 3 | Selecting suitable solid organ transplant donors: Reducing the risk of donor-transmitted infections显示文摘Selection of the appropriate donor is essential to a successful allograft recipient outcome for solid organ transplantation. Multiple infectious diseases have been transmitted from the donor to the recipient via transplantation. Donor-transmitted infections cause increased morbidity and mortality to the recipient. In recent years, a series of high-profile transmissions of infections have occurred in organ recipients prompt-ing increased attention on the process of improving the selection of an appropriate donor that balances the shortage of needed allografts with an approach that mitigates the risk of donor-transmitted infection to the recipient. Important advances focused on improving donor screening diagnostics, using previously excluded high-risk donors, and individualizing the selection of allografts to recipients based on their prior infection history are serving to increase the donor pool and improve outcomes after transplant. This article serves to review the relevant literature surrounding this topic and to provide a suggested approach to the selection of an appropriate solid organ transplant donor. | Christopher S Kovacs Jr Christine E Koval David van Duin Amanda Guedes de Morais Blanca E Gonzalez Robin K Avery Steven D Mawhorter Kyle D Brizendine Eric D Cober Cyndee Miranda Rabin K Shrestha Lucileia Teixeira Sherif B Mossad | 2014 | World Journal of Transplantation2014，4，2： | 7 |
| 4 | Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells显示文摘AIM: To study the therapeutic effect of three tubeguides with electrical conductivity associated to mesenchymal stem cells(MSCs) on neuro-muscular regeneration after neurotmesis.METHODS: Rats with 10-mm gap nerve injury were tested using polyvinyl alcohol(PVA), PVA-carbon nanotubes(CNTs) and MSCs, and PVA-polypyrrole(PPy). The regenerated nerves and tibialis anterior muscles were processed for stereological studies after 20 wk. The functional recovery was assessed serially for gait biomechanical analysis, by extensor postural thrust, sciatic functional index and static sciatic functionalindex(SSI), and by withdrawal reflex latency(WRL). In vitro studies included cytocompatibility, flow cytometry, reverse transcriptase polymerase chain reaction and karyotype analysis of the MSCs. Histopathology of lung, liver, kidneys, and regional lymph nodes ensured the biomaterials biocompatibility. RESULTS: SSI remained negative throughout and independently from treatment. Differences between treted groups in the severity of changes in WRL existed, showing a faster regeneration for PVA-CNTs-MSCs(P < 0.05). At toe-off, less acute ankle joint angles were seen for PVA-CNTs-MSCs group(P = 0.051) suggesting improved ankle muscles function during the push off phase of the gait cycle. In PVA-PPy and PVA-CNTs groups, there was a 25% and 42% increase of average fiber area and a 13% and 21% increase of the 'minimal Feret's diameter' respectively. Stereological analysis disclosed a significantly(P < 0.05) increased myelin thickness(M), ratio myelin thickness/axon diameter(M/d) and ratio axon diameter/fiber diameter(d/D; g-ratio) in PVA-CNT-MSCs group(P < 0.05). CONCLUSION: Results revealed that treatment with MSCs and PVA-CNTs tube-guides induced better nerve fiber regeneration. Functional and kinematics analysis revealed positive synergistic effects brought by MSCs and PVA-CNTs. The PVA-CNTs and PVA-PPy are promising scaffolds with electric conductive properties, bio- and cytocompatible that might prevent the secondary neurogenic muscular atrophy by improving the reestablishment of the neuro-muscular junction. | Jorge Ribeiro Tiago Pereira Ana Rita Caseiro Paulo Armada-da-Silva Isabel Pires Justina Prada Irina Amorim Sandra Amado Miguel Franca Carolina Goncalves Maria Ascensao Lopes Jose Domingos Santos Dina Morais Silva Stefano Geuna Ana Lúcia Luís Ana Colette Maurício | 2015 | World Journal of Stem Cells2015，7，6： | 2 |
| 5 | Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering显示文摘The complex meniscus tissue plays a critical role in the knee. The high susceptibility to injury has led to an intense pursuit for better tissue engineering regenerative strategies, where scaffolds play a major role. In this study, indirect printed hierarchical multilayered sca ffolds composed by a silk fibroin (SF) upper layer and an 80/20 (w/w) ratio of SF/ionic-doped β-tricalcium phosphate (TCP) bottom layer were developed. Furthermore, a comparative analysis between two types of sca ffolds pro- duced using di fferent SF concentrations, i.e., 8% (w/v) (Hi8) and 16% (w/v) (Hi16) was performed. In terms of architecture and morphology, the produced sca ffolds presented homogeneous porosity in both layers and no di fferences were observed when comparing both sca ffolds. A decrease in terms of mechanical performance of the sca ffolds was observed when SF concentration decreased from 16 to 8% (w/v). Hi16 revealed a static compressive modulus of 0.66 ± 0.05 MPa and dynamical mechanical properties ranging from 2.17 ± 0.25 to 3.19 ± 0.38 MPa. By its turn, Hi8 presented a compressive modulus of 0.27 ± 0.08 MPa and dynamical mechanical properties ranging from 1.03 ± 0.08 MPa to 1.56 ± 0.13 MPa. In vitro bioactivity studies showed formation of apatite crystals onto the surface of Hi8 and Hi16 bottom layers. Human meniscus cells (hMCs) and human primary osteoblasts were cultured separately onto the top layer (SF8 and SF16) and bottom layer (SF8/TCP and SF16/TCP) of the hierarchical sca ffolds Hi8 and Hi16, respectively. Both cell types showed good adhesion and proliferation as denoted by the live/dead staining, Alamar Blue assay and DNA quanti fication analysis. Subcutaneous implantation in mice revealed weak in flammation and sca ffold’s integrity. The hierarchical indirect printed SF sca ffolds can be promising candidate for meniscus TE sca ffolding applications due their suitable mechanical properties, good biological performance and possibility of being applied in a patient-speci fic approach. | Joao BCosta Joana Silva-Correia Sandra Pina Alain da Silva Morais Sílvia Vieira Hélder Pereira Joao Espregueira-Mendes Rui LReis Joaquim M.Oliveira | 2019 | Bio-Design and Manufacturing2019，2，4： | 2 |
| 6 | Identification and characterization of phenylpyruvate decarboxylase genes in Saccharomyces cerevisiae显示文摘 | VURALHAN Z MORAIS M A TAI S L | 2003 | Applied and Environmental Microbiology2003，69，8： | 1 |
| 7 | Phenolic profile of seventeen Portuguese wild mushrooms显示文摘 | Josiana A. Vaz Lillian Barros Anabela Martins Jorge Sá Morais M. Helena Vasconcelos Isabel C.F.R. Ferreira | 2010 | LWT - Food Science and Technology2010，，1： | 1 |
| 8 | Process development for the recovery of high-grade lanthanum by solvent extraction显示文摘 | Morais C A Ciminelli V S T | 2004 | Hydrometallurgy2004，73，： | 1 |
| 9 | Liver transplantation in management of alveolar echinococcosis: two case reports显示文摘 | Moray G Shahbazov R Sevmis S | 2009 | Transplant Proc2009，41，7： | 1 |
| 10 | Combined effects of chemical dip and/or carrageenan coating and/or controlled atmo- sphere on quality of fresh-cut banana显示文摘 | BICO S L S RAPOSO M F J MORAIS R M S C | 2009 | Food Control2009，20，5： | 1 |
| 11 | Synthesis of Sensitive Immunoassay for Tetracycline Rresidues Application to Honey Samples 显示文摘 | Pastor-Navarro N Morais S Haptens and Development of a Maquieira A | 2007 | Analytica Chimica Acta2007，594，： | 1 |
| 12 | Center/periphery imbalance in tourism development : the case of Taiwan 显示文摘 | Morais D B Lin C H Hou J S | 2004 | Tourism Analysis2004，9，4： | 1 |
| 13 | Process development for the recovery of high-grade lanthanum by solvent extraction显示文摘 | Morais C A Ciminelli V S T | 2004 | Hydrometallurgy2004，73，34： | 1 |
| 14 | Nitriding of titanium disks and industrial dental implants using hollow cathode discharge显示文摘 | Jr ALVES C GUERRA NETO C L B MORAIS G H S | 2006 | Surface and Coatings Technology2006，200，： | 1 |
| 15 | Development and characterization of novel alginate-based hydrogels as vehicles for bone substitutes 显示文摘 | MORAIS D S RODRIGUES M A SILVA T I | 2013 | Carbohydr Polym2013，95，： | 1 |
| 16 | Recovery of europium from a rare earth chloride solution显示文摘 | Morais C A Ciminelli V S T | 1998 | Hydrometallurgy1998，49，： | 1 |
| 17 | CB be- havior in reactor switching: Applicability and limitations of the concept of chopping number显示文摘 | PORTELA C M MORAIS S A TEIXERIA J S | 1988 | IEEE Transactions on Power Delivery1988，3，3： | 1 |
| 18 | Recovery of europium by chemical reduction of a commercial solution of europium and gadolinium chlorides 显示文摘 | Morais C A Ciminelli V S T | 2001 | Hydrometallurgy2001，60，： | 1 |
| 19 | Multiplexed microimmunoassays on a digital versatile disk显示文摘 | Morais S Tortajada-Genaro LA Arnandis-Chover T | | 0，，14： | 1 |
| 20 | A ZigBeeMulti-powered Wireless Acquisition Device for Remote SensingApplications in Precision Viticulture显示文摘 | MORAIS R FEMANDES M A MATOS S G | 2008 | Computers and Electron-ics in Agriculture2008，62，2： | 1 |
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