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| 1 | Concomitant-chemoradiotherapy-associated oral lesions in patients with oral squamous-cell carcinoma显示文摘Objective:Oral squamous-cell carcinoma(OSCC)accounts for >90% of oral cancers affecting adults mostly between the fourth to seventh decades of life.The most common OSCC treatment is concomitant chemoradiotherapy(CCRT)having both locoregional and distant control,but CCRT has acute and chronic toxic effects on adjacent normal tissue.This study aimed to determine the side effects of CCRT on the oral mucosa and to characterize the clinicopathology of oral lesions in patients with OSCC.Methods:This descriptive,cross-sectional study was certified by the Ethical Review Committee(UHS/Education/126-12/2728)of the University of Health Sciences,Lahore,Pakistan.OSSC patients(n=81)with various histological subtypes,grades,and stages were recruited,and findings on their oral examination were recorded.These patients received 70,90,and 119 Gy of radiotherapy dosages in combination with the chemotherapy drugs cisplatin and 5-fluorouracil.Data were analyzed using SPSS 20.0.Results:The most common presentation of OSCC was a nonhealing ulcer(63%) involving tongue(55.6%).Clinical findings included mucositis(92.6%)and xerostomia of mild,moderate,and severe degrees in 11.1%,46.9%,and 35.8% cases,respectively.Ulcers(87.7%),palpable lymph nodes(64.2%),limited mouth opening(64.2%)and fistula(40.7%) were also observed.In females,the association of radiotherapy dosage with limited mouth opening,xerostomia,and histological grading was statistically significant(P<0.05).The association of chemotherapy drugs with xerostomia(P=0.003)was also statistically significant.Conclusions:CCRT induced mucositis,xerostomia,and trismus in patients with OSCC. | Sadia Minhas Muhammad Kashif Wasif Altaf Nadeem Afzal Abdul Hanan Nagi | 2017 | Cancer Biology & Medicine2017，14，2： | 6 |
| 2 | Influence of Seed Priming on Performance and Water Productivity of Direct Seeded Rice in Alternating Wetting and Drying显示文摘Direct seeded rice is promising alternative to traditional transplanting, but requires appropriate crop and water management to maintain yield performance and achieve high water productivity. Present study evaluated the effect of seed priming and irrigation on crop establishment, tillering, agronomic traits, paddy yield, grain quality and water productivity of direct seeded rice in alternate wetting and drying(DSR-AWD) in comparison with direct seeded rice at field capacity(DSRFC). Seed priming treatments were osmo-priming with KCl(2.2%), Ca Cl2(2.2%) and moringa leaf extracts(MLE, 3.3%) including hydro-priming as control. Among the treatments, seed osmo-primed with MLE emerged earlier and had higher final emergence, followed by osmo-priming with Ca Cl2. Tillering emergence rate and number of tillers per plant were the highest for seed priming with Ca Cl2 in DSRAWD. Total productive and non-productive tillers, panicle length, biological and grain yields, harvest index were highest for seed priming with MLE or Ca Cl2 in DSR-AWD. Similarly, grain quality, estimated in terms of normal grains, abortive and chalky grains, was also the highest in DSR-AWD with MLE osmo-priming. Benefit cost ratio and water productivity was also the highest in DSR-AWD for seed priming with MLE. In conclusion, seed priming with MLE or Ca Cl2 can be successfully employed to improve the direct seeded rice performance when practiced with alternate wetting and drying irrigation. | Hafeez Ur REHMAN Muhammad KAMRAN Shahzad Maqsood Ahmed BASRA Irfan AFZAL Muhammad FAROOQ | 2015 | Rice science2015，22，4： | 5 |
| 3 | Electronic-property dependent interactions between tetracycline and graphene nanomaterials in aqueous solution显示文摘Understanding the interactions between graphene nanomaterials(GNMs) and antibiotics in aqueous solution is critical to both the engineering applications of GNMs and the assessment of their potential impact on the fate and transport of antibiotics in the aquatic environment. In this study, adsorption of one common antibiotic, tetracycline, by graphene oxide(GO) and reduced graphene oxide(RGO) was examined with multi-walled carbon nanotubes(MWCNTs) and graphite as comparison. The results showed that the tetracycline adsorption capacity by the four selected carbonaceous materials on the unit mass basis followed an order of GO > RGO > MWCNTs > graphite. Upon normalization by surface area,graphite, RGO and MWCNTs had almost the same high tetracycline adsorption affinity while GO exhibited the lowest. We proposed π-electron-property dependent interaction mechanisms to explain the observed different adsorption behaviors. Density functional theory(DFT) calculations suggested that the oxygen-containing functional groups on GO surface reduced its π-electron-donating ability, and thus decreased the π-based interactions between tetracycline and GO surface. Comparison of adsorption efficiency at different p H indicated that electrostatic interaction also played an important role in tetracycline-GO interactions. Site energy analysis confirmed a highly heterogeneous distribution of the binding sites and strong tetracycline binding affinity of GO surface. | Lin He Fei-fei Liu Mengyao Zhao Zhen Qi Xuefei Sun Muhammad Zaheer Afzal Xiaomin Sun Yanhui Li Jingcheng Hao Shuguang Wang | 2018 | Journal of Environmental Sciences2018，30，4： | 4 |
| 4 | Qualitatively and quantitatively assessing the aggregation ability of sludge during aerobic granulation process combined XDLVO theory with physicochemical properties显示文摘Inexact mechanism of aerobic granulation still impedes optimization and application of aerobic granules. In this study, the extended Derjaguin, Landau, Verwey, and Overbeek(XDLVO) theory and physicochemical properties were combined to assess the aggregation ability of sludge during aerobic granulation process qualitatively and quantitatively. Results show that relative hydrophobicity of sludge and polysaccharide content of extracellular polymeric substances(EPS) increased, while electronegativity of sludge decreased during acclimation phase. After 20 days' acclimation, small granules began to form due to high aggregation ability of sludge. Since then, coexisted flocs and granules possessed distinct physicochemical properties during granulation and maturation phase. The relative hydrophobicity decreased while electronegativity increased for flocs, whereas that for granules presented reverse trend. Through analyzing the interaction energy using the XDLVO theory, small granules tended to self-grow rather than self-aggregate or attach of flocs due to poor aggregation ability between flocs and granules during the granulation phase. Besides, remaining flocs were unlikely to self-aggregate owing to poor aggregation ability, low hydrophobicity and high electronegativity. | Shasha Yuan Mingming Gao Hui Ma Muhammad Zaheer Afzal Yun-Kun Wang Mingyu Wang Hai Xu Shu-Guang Wang Xin-Hua Wang | 2018 | Journal of Environmental Sciences2018，30，5： | 4 |
| 5 | Seed Priming Influence on Early Crop Growth, Phenological Development and Yield Performance of Linola (Linum usitatissimum L.)显示文摘Reduced early crop growth and limited branching are amongst yield limiting factors of linola. Field response of seed priming treatments viz. 50 mmol L-1 salicylic acid(SA), 2.2% CaCl2 and 3.3% moringa leaf extract(MLE) including untreated dry and hydropriming controls was evaluated on early crop growth and yield performance of linola. Osmopriming with CaCl2 reduced emergence time and produced the highest seedling fresh and dry weights including Chl. a contents. Osmopriming with CaCl2reduced crop branching and fl owering and maturity times and had the maximum plant height, number of branches, tillers, pods and seeds per pod followed by MLE. Increase in seed weight, biological and seed yields was 9.30, 34.16 and 39.49%, harvest index(4.12%) and oil contents(13.39%) for CaCl2 osmopriming. Positive relationship between emergence and seedling vigor traits, 100-seed weight, seed yield with maturity time, 100-seed weight and seed yield were found. The study concludes that seed osmopriming with CaCl2 or MLE can play signifi cant role to improve early crop growth and seed yields of linola. | Hafeez ur Rehman Muhammad Qaiser Nawaz Shahzad Maqsood Ahmed Basra Irfan Afzal Azra Yasmeen Fayyaz ul-Hassan | 2014 | Journal of Integrative Agriculture2014，13，5： | 4 |
| 6 | Seed priming improves early seedling vigor, growth and productivity of spring maize显示文摘Potential of seed priming treatments in improving the performance of early planted maize was evaluated against timely planting. Seeds of maize hybrid FH-810 were soaked in water(hydropriming), Ca Cl2(2.2%, osmopriming), moringa leaf extracts(MLE 3.3%, osmopriming) and salicylic acid(SA, 50 mg L–1, hormonal priming) each for 18 h. Untreated and hydroprimed seeds were taken as control. Seeds primed with SA took less time in emergence and had high vigor in early planted maize. Amongst treatments, hormonal priming, reduced the electrical conductivity, increased the leaf relative and chlorophyll contents followed by osmopriming with Ca Cl2 at seedling stage. Likewise, plant height, grain rows and 1 000-grain weight, grain and biological yield and harvest index were also improved by seed priming; however hormonal priming and osmopriming with MLE were more effective in this regard. Improved yield performance by hormonal priming or osmopriming with MLE in early planting primarily owed to increased leaf area index, crop growth and net assimilation rates, and maintenance of green leaf area at maturity. In conclusion, osmopriming with MLE and hormonal priming with SA were the most economical treatments in improving productivity of early planted spring maize through stimulation of early seedling growth at low temperature. | Hafeez ur Rehman Hassan Iqbal Shahzad M A Basra Irfan Afzal Muhammad Farooq Abdul Wakeel WANG Ning | 2015 | Journal of Integrative Agriculture2015，14，9： | 3 |
| 7 | Antimicrobial chemical constituents from endophytic fungus Phoma sp.显示文摘Objective:To evaluate the antiniicrobial polenlial of different extracts of the endophytic fungus Phoma sp.and the tentative identification of their active constituents.Methods:The extract and compounds were screened for antimicrobial activity using the Agar Well Diffusion Method.Four compounds were purified using column chromatography and tlieir structures were assigned using~1H and~(13)C NMR spectra,DEPT,2D COSY,HMQC and HMBC experiments.Results:The ethyl acetate fraction of Phoma sp.showed good antifungal,antibacterial,and algicidal properties.One new dihydrofuran derivative,named phomafuranol(1),together with tliree known compounds,phomalacton(2),(3R)-5-hydroxymellein(3)and emodin(4)were isolated from the ethyl acetate fraction of Phoma sp.Preliminary studies indicated that phomalacton(2)displayed strong antibacterial,good antifungal and antialgal activities.Similarly(3R)-5-hydroxymellein(3)and emodin(4)showed good antifungal,antibacterial and algicidal properties.Conclusions:Antimicrobial activities of the ethyl acetate fraction of the endophytic fungus Phoma sp.and isolated compounds clearly demonstrate that Phoma sp.and its active compounds represent a great potential for the food,cosmetic and pharmaceutical industries. | Hidayat Hussain Ines Kock Ahmed Al-Harrasi Ahmed Al-Rawahi Ghulam Abbas Ivan R.Green Afzal Shah Amin Badshah Muhammad Saleem Siegfried Draeger Barbara Schulz Karsten Krohn | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，9： | 3 |
| 8 | Mutagenic and cytotoxic potential of Endosulfan and Lambda-cyhalothrin—In vitro study describing individual and combined effects of pesticides显示文摘Excessive use of pesticides poses increased risks to non target species including humans. In the developing countries, lack of proper awareness about the toxic potential of pesticides makes the farmer more vulnerable to pesticide linked toxicities, which could lead to diverse pathological conditions. The toxic potential of a pesticide could be determined by their ability to induce genetic mutations and cytotoxicity. Hence, determination of genetic mutation and cytotoxicity of each pesticide is unavoidable to legislate health and safety appraisal about pesticides. The objective of current investigation was to determine the genotoxic and cytotoxic potential of Endosulfan(EN) and Lambda-cyhalothrin(LC); individually and in combination. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide(MTT) assay was utilized to determine cytotoxicity, while two mutant histidine dependent Salmonella strains(TA98, TA100) were used to determine the mutagenicity of EN and LC.Moreover, mutagenicity assay was conducted with and without S9 to evaluate the effects of metabolic activation on mutagenicity. Even though a dose dependent increase in the number of revertant colonies was detected with EN against both bacterial strains, a highly significant(p < 0.05) increase in the mutagenicity was detected in TA98 with S9. In comparison, data obtained from LC revealed less mutagenic potential than EN. Surprisingly,the non-mutagenic individual-concentrations of EN and LC showed dose dependent mutagenicity when combined. Combination of EN and LC synergistically induced mutagenicity both in TA98 and TA100. MTT assay spotlighted comparable dose dependent cytotoxicity effects of both pesticides. Interestingly, the combination of EN and LC produced increased reversion and cytotoxicity at lower doses as compared to each pesticide, concluding that pesticide exposure even at sub-lethal doses can produce cytotoxicity and genetic mutations, which could lead to carcinogenicity. | Umber Saleem Sohail Ejaz Muhammad Ashraf Muhammad Ovais Omer Imran Iltaf Zainab Batool Riffat Fatima Msbah Afzal | 2014 | Journal of Environmental Sciences2014，26，7： | 3 |
| 9 | Non-hypoxic stabilization of HIF-Iα during coordinated interaction between Akt and angiopoietin-1 enhances endothelial commitment of bone marrow stem cells显示文摘 | Vien Lai Muhammad Afzal Muhammad Ashraf Shujia Jiang Husnain Haider | 2012 | Journal of Molecular Medicine2012，，6： | 2 |
| 10 | Diagnostically untypable hepatitis C virus variants:It is time to resolve the problem显示文摘Pakistan is a low income country with more than 10million hepatitis C virus(HCV)infections and the burden is on continuous raise.Accurate viral genotyping is very critical for proper treatment of the infected individuals as the sustained virological response of the standard antiviral interferon therapy is genotype dependent.We observed at our diagnostic center that15.6%of HCV patient’s samples were not genotypeable by using Ohno et al method.The genotyped samples showed that 3a(68.3%)is the major prevalent genotype in Pakistan followed by 2a(10.3%),3b(2.6%),1b(1.5%),2b(1.2%)and 1a(0.5%).Presence of large number of untypable HCV variants in the current study highlights an important issue of health care setup in Pakistan.Untypable HCV cases create difficulties in treatment of these patients.The problem of routine diagnostics setup of Pakistan should be addressed on priority basis to facilitate the medical professionals in patient’s treatment and to help in achieving the maximum sustained virological response. | Muhammad Sohail Afzal Muhammad Yousaf Khan Muhammad Ammar Sadia Anjum Najm us Sahar Sadaf Zaidi | 2014 | World Journal of Gastroenterology2014，20，46： | 2 |
| 11 | 基于RFID技术的农田机井灌溉管理系统设计显示文摘针对农田机井灌溉系统效率低下、管理较差的问题,提出了一种基于RFID技术的农田机井灌溉管理系统,该系统利用嵌入式微控制器LPC2103及射频芯片MF-RC522来构建,通过射频IC卡作为信息传输的媒介,以达到节水及提高效率的目的。针对传统射频IC卡易被复制,信息易被获取等问题,提出了一种新的安全认证协议,该协议确保了卡片的安全性,使该系统能够被广泛推广。实际应用表明,该系统运行良好,切实可行。 | 党倩滢 淡丹 杨文健 Muhammad Mannan Afzal Fazli Hameed | 2017 | 电子设计工程2017，25，20： | 2 |
| 12 | Systematic study of(MoTa)_(x)NbTiZr medium-and high-entropy alloys for biomedical implants-In vivo biocompatibility examination显示文摘In this study,the application of medium-and high-entropy(Mo Ta)_(x)Nb Ti Zr alloys in biomedical implants was systematically analyzed.The alloy with the best combination of mechanical properties was selected and characterized for in vitro and in vivo response for the first time to examine its biomedical properties.A logarithmic increase in the hardness and the yield strength was observed as a function of the Mo and Ta content.Alloys with up to 0.4 mol fraction of Mo and Ta showed a plastic strain of more than 30%under compression.The nanoindentation results showed that the addition of Mo and Ta increased the elastic modulus of the system linearly.It was surmised that the addition of Ta and Mo above a critical concentration(mole fraction=0.4)was unfavorable from a biomedical perspective as it increased the brittleness and elastic modulus and decreased the ductility of the system.Therefore,the(Mo Ta)_(0.2)Nb Ti Zr alloy is a potential structural material for biomedical implants because of its excellent strength and ductility.The developed alloy was investigated for its corrosion properties and compared with commercial biomedical alloys.Furthermore,the biocompatibility of the alloy was examined using an in vivo examination.The alloy was implanted in the skeletal muscles of mice for four weeks and the histology of the surrounding tissue was studied.The alloy exhibited strong passive behavior in a phosphate buffer solution and non-toxic soft tissue response. | Muhammad Akmal Ahtesham Hussain Muhammad Afzal Young Ik Lee Ho Jin Ryu | 2021 | Journal of Materials Science & Technology2021，，19： | 2 |
| 13 | Why more research needs to be done on echinococcosis in Pakistan显示文摘Background:Echinococcosis has a worldwide geographical distribution with endemic foci on every inhabited continent.Due to the frequent outbreaks in different parts of Pakistan in the recent past,echinococcosis is being described as a neglected tropical disease and is considered one of the most neglected parasitic diseases in the country.In endemic regions,predominantly settings with limited resources,there are high numbers of echinococcosis patients,as these communities do not have access to appropriate treatment.In Pakistan,there are limited reports on echinococcosis.The disease is prevalent in human and livestock,but this has not been sufficiently explored yet.Pakistan is an agricultural country and due to the disease’s zoonotic mode of transmission,there is a dire need of future research on it.The present paper is an effort to highlight the importance of echinococcosis in Pakistan.Discussion:There is a dire need for future research on echinococcosis in Pakistan as very few investigations had been carried out on this topic thus far.The prevalence of the disease in neighbouring countries highlights that Pakistan might be at severe risk of this zoonotic infection and further supports the need for more research.In Pakistan,the majority of the population lives in rural areas with limited acess to proper hygienic/sanitary facilities.These conditions favour the outbreak of diseases such as echinococcosis.The limited available data could result in higher outbreaks in the future,and thus cause the already weak healthcare system to overburden.The country has a meagre annual budget for health,which is being spent on known infections such as polio,dengue fever and hepatic viral infections.A proper surveillance system for echinococcosis is required across the country as treatment is usually expensive,complicated and may require extensive surgery and/or prolonged drug therapy.Development of new/novel drugs and other treatment modalities receives very little,if any,attention.Prognostic awareness programmes against this infection involve deworming of the infected animals,improved food inspection and slaughterhouse hygiene,and public education campaigns.Conclusion:Future research on echinococcosis is anticipated to demonstrate whether the epidemiology,diagnosis and recombinant vaccines/antibodies relating to echinococcosis can meet the quality standards(purity,potency,safety and efficacy)defined by the World Health Organization.Research work should be carried out on the epidemiology and serodiagnosis of echinocossis in the different areas of Pakistan,which will be useful for the proper eradication of echinococcosis in this region.The health department should implement awareness-raising campaigns for the general public in order to reduce the burden of disease. | Haroon Ahmed Shahzad Ali Muhammad Sohail Afzal Abid Ali Khan Hamid Raza Zaheer Hussain Shah Sami Simsek | 2017 | Infectious Diseases of Poverty2017，6，1： | 2 |
| 14 | Anxiolytic potential of ursolic acid derivative-a stearoyl glucoside isolated from Lantana camara L.(verbanaceae)显示文摘Objective:To investigate the anxiolytic activity of newly isolated compound by our lab called ursolic acid stearoyl glucoside(UASG) from the leaves of Lantana camam(L camam).Methods: Column chromatography was used to isolate UASG.Anxiolytic potential was experimentally proved and demonstrated through Elevated plus-maze,Open field and light and dark test. Results:The UASG showed marked increased in time spent(%) and number of frequent movements made by animals in open arm of elevated plus-maze apparatus.In light and dark model,UASG produced marked increase in time spent by animal,number of crossing and reduced duration of immobility in light box.Conclusions:UASG showed significant increase in number of rearing,assisted rearing and number of square crossed in open field established test model.UASG showed its anxiolytic effect in dose dependent manner. | Imran Kazmi Muhammad Afzal Babar Ali Zoheir A.Damanhouri Aftab Ahmaol Firoz Anwar | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，6： | 2 |
| 15 | The changing epidemiology pattern and frequency distribution of hepatitis C virus in Pakistan显示文摘 | Sadia Butt Muhammad Idrees Haji Akbar Irshad ur Rehman Zunaira Awan Samia Afzal Abrar Hussain Muhammad Shahid Sobia Manzoor Shazia Rafique | 2010 | Infection Genetics and Evolution2010，，5： | 2 |
| 16 | Botanicals, selective insecticides, and predators to control Diaphorina citri (Hemiptera: Liviidae) in citrus orchards显示文摘引起 huanglongbing (HLB ) 或破坏的柠檬 greening 和经济地重要的疾病的亚洲柠檬 psyllid (ACP ) Diaphorina citri Kuwayama 向量病原体在大多数柠檬成长区域介绍。年轻柠檬射击为 psyllid 复制和开发被要求。在冬季期间,柠檬树生产很少或没有新生长。Overwintering 成年人在对另外的害虫和有益的昆虫也吸引人的最新新兴的射击上在春天复制。Botanicals 和相对选择的杀虫药剂能帮助保存有益的昆虫并且把害虫抵抗归结为杀虫药剂。Azadirachtin (Neem ) 的水花, Tropane (曼陀罗属) , Spirotetramat, Spinetoram,和用途广泛的 Imidacloprid 被评估在 10 岁的 “Kinow” 上在春天和夏天控制 ACP生产新生长的柠檬 reticulata Blanco 树。Psyllid 人口高在处理申请前每样品正在平均 5-9 美女或成年人。美女或成年人显著地在所有处理每样品被归结为 0.5-1.5 3 个星期,平均 61%-83% 减小。在 ladybeetles Adalia bipunctata, Aneglei scardoni, Cheilomenes sexmaculata,和 Coccinella septempunctata 的重要减小都没被观察。除了与 Tropane, Syrphids,蜘蛛和绿草蜻蛉在对待的树上被减少。研究被保证在有杀虫药剂的 ACP 和相互作用上估计这些食肉动物的影响。在 ACP 人口的观察减小不能在 HLB 的传播就它的繁殖潜力和角色而言是足够的。后续水花可以被要求用不同杀虫药剂的旋转完成另外的抑制。 | Azhar A. Khan Muhammad Afzal Jawwad A. Qureshi Arif M. Khan Abubakar M. Raza | 2014 | Insect Science2014，21，6： | 2 |
| 17 | Antiulcer and antioxidant activities of a new steroid from Morus alba显示文摘 | Aftab Ahmad Gaurav Gupta Muhammad Afzal Imran Kazmi Firoz Anwar | 2012 | Life Sciences2012，，： | 1 |
| 18 | Proteomics analysis of Trichoplusia ni midgut epithelial cell brush border membrane vesicles显示文摘The insect midgut epithelium is composed of columnar, goblet, and regenerative cells. Columnar epithelial cells are the most abundant and have membrane protrusions that form the brush border membrane (BBM) on their apical side. These increase surface area available for the transport of nutrients, but also provide opportunities for interaction with xenobiotics such as pathogens, toxins and host plant allelochemicals. Recent improvements in proteomic and bioinfbrmatics tools provided an opportunity to determine the proteome of the T. ni BBM in unprecedented detail. This study reports the identification of proteins from BBM vesicles (BBMVs) using single dimension polyacrylamide gel elec? trophoresis coupled with multi-dimensional protein identification technology. More than 3000 proteins were associated with the BBMV of which 697 were predicted to possess either a signal peptide, at least one transmembrane domain or a GPI-anchor signal. Of these, bioinfbrmatics analysis and manual curation predicted that 185 may be associated with the BBMV or epithelial cell plasma membrane. These are discussed with respect to their predicted functions, namely digestion, nutrient uptake, cell signaling, development, cell-cell interactions, and other functions. We believe this to be the most detailed proteomic analysis of the lepidopteran midgut epithelium membrane to date, which will provide information to better understand the biochemical, physiological and pathological processes taking place in the larval midgut. | Muhammad Afzal Javed Cathy Coutu David A. Theilmann Martin A. Erlandson Dwayne D. Hegedus | 2019 | Insect Science2019，26，3： | 1 |
| 19 | Emicrowave drying of wastewater sludge: Experimental and modeling study显示文摘 | Lyes Bennamoun Zhenyu Chen Muhammad T Afzal | 2015 | Drying Technol2015，34，2： | 1 |
| 20 | Modeling of moisture diffu- sion in microwave drying of hardwood显示文摘 | Jia Donghua Muhammad T Afzal | 2007 | Drying Technology2007，25，3： | 1 |
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