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| 1 | Role of AXL in invasion and drug resistance of colon and breast cancer cells and its association with p53 alterations显示文摘AIM To characterize AXL receptor tyrosine kinase(AXL)expression in relationship to tumor protein P53(TP53gene,p53 protein)and its role in tumor invasion and response to therapy.METHODS We used 14 cell lines,including 3 isogenic pairs carrying mutant/knockout p53,to gain insight into the relationship between AXL and TP53.These included HCT116,HCT116.p53 mutant,RKO,and RKO.p53-/-lines(all from colon cancers)as well as breast cancer cell lines MCF7 and 1001(MCF7-p53 mutant clone).He La cell line was used as a positive control for epithelial to mesenchymal transition(EMT).AXL expression was determined by Western blotting using rabbit monoclonal antibody clone C89E7.AXL si RNA silencing was performed and followed by collagen invasion assay.Cell viability analysis using the sulforhodamine B assay and the invasion assay were performed after exposure to chemotherapeutic agents(doxorubicin for breast cancer cells;5FU or irinotecan for colon cancer cells).RESULTS We showed that the introduction of p53 mutations or knockout increased expression levels of AXL in isogenic cells compared to the matching p53 wild-type parental cells.Overall,we found a trend for correlation between the potential EMT candidate AXL,p53 alterations,and EMT markers in colorectal and breast cancers.The expression of AXL in RKO cells,a rare colon cancer cell line with inactive Wnt signaling,suggests that the AXL oncogene might provide an alternative genetic pathway for colorectal carcinogenesis in the absence of Wnt signaling activation and TP53 mutation.AXL silencing in the TP53 mutant isogenic cell lines 1001,HCT116.p53 mutant and RKO.P53-/-was>95%efficient and the silenced cells were less invasive compared to the parental TP53 wild-type cells.AXL silencing showed a subtle trend to restore colon cancer cell sensitivity to5FU or irinotecan.Importantly,AXL expressing cells developed more invasive potential after exposure to chemotherapy compared to the AXL-silenced cells.CONCLUSION AXL is influenced by p53 status and could cause the emergence of aggressive clones after exposure to chemotherapy.These findings could have applications in cancer management. | Wael M Abdel-Rahman Noura A Al-khayyal Vidhya A Nair S R Aravind Maha Saber-Ayad | 2017 | World Journal of Gastroenterology2017，23，19： | 7 |
| 2 | Immunohistochemical assessment of localization and frequency of micremetastasis in lymph nodes of colorectal cancer显示文摘 | Noura S Yamamoto H Miyake Y | 2002 | Clin Cancer Res2002，8，3： | 1 |
| 3 | Tumor markers for colorectal cancer显示文摘 | Yamamoto H Miyake Y Noura S | 2001 | Gan To Kagaku Ryoho2001，28，： | 1 |
| 4 | Comparative detection of lymph node mierometastases of stage Ⅱ colorectal cancer by reverse transcriptase polymerase chain reaction and immunohistoehemistry显示文摘 | Noura S Yamamoto H Ohnishi T | 2002 | J Clin Oncol2002，20，20： | 1 |
| 5 | A case of apocrine adenocarcinoma suspected as an axillary lymph node metastasis from cecal cancer显示文摘 | Ogino T Noura S Ohue M | | 0，，： | 1 |
| 6 | A case of chemoradiation with tissue expander for local reccuience Jrectal cancer显示文摘 | Tanaka K Noura S Ohue M | 2005 | Can To Kagaku Ryoho2005，32，11： | 1 |
| 7 | Super-Efficiency in DEA by Effectiveness of Each Unitin Society显示文摘 | Noura A A Hosseinzadeh L F Jahanshahloo G R Fanati R S | 2011 | Applied Mathematics Letters2011，24，5： | 1 |
| 8 | Evaluation of the lateral sentinel node by indoeyanine green for rectal cancer based on mierometastasis determined by reverse transcriptase-polymerase chain reaction 显示文摘 | Noura S Ohue M Seki Y | 2008 | Oncol Rep2008，20，4： | 1 |
| 9 | Immunohistochemical assessment of localization and frequency of micrometastases in lymph nodes of colorectal cancer显示文摘 | Noura S Yamamoto H Miyake Y | 2002 | Clin Cancer Res2002，8，23： | 1 |
| 10 | Expr-ession of second class of KIP protein D57kip2 in human colorectal carcinoma显示文摘 | NOURA S YAMAMOTO H SEKIMOTO M | 2001 | Int J Oncol2001，1991，1： | 1 |
| 11 | Immunohistochemical assessment of localization and frequency of micrometastases in lymph nodes of colorectal cancer显示文摘 | Noura S Yamamoto H Miyake Y | | Clinical Cancer Research0，，： | 1 |
| 12 | Expression of second class of KIP protein p57KIP2 in human colorectal carcinoma显示文摘 | Noura S Yamamoto H Sekimoto M | 2001 | Int J Oncol2001，19，： | 1 |
| 13 | Tumor markers for colorectal cancer 显示文摘 | Yamamoto H Miyake Y Noura S | 2001 | Gan To K agaku Ryoho2001，28，9： | 1 |
| 14 | Robust fault diagnosis for quadrotor UAVs using adaptive Than observer显示文摘 | Cen Z H Noura H Tri B S | 2014 | Journal of Intelligent & Robotic Systems2014，73，： | 1 |
| 15 | L tumormarkers for color- ec - tal cancer显示文摘 | YAMAMOTOH MIYAKEY NOURA S | 2001 | GAN Tokagaku Ryoho2001，28，9： | 1 |
| 16 | Immunohistochemical assessment of localization and frequency of micrometastasis in lymph nodes of colorectal cancer显示文摘 | Noura S Yamamoto H Miyake Y | 2002 | Clin Cancer Res2002，8，3： | 1 |
| 17 | Evaluation of the lateral sentinel node by indocyanine greenfor rectal cancer based on micrometastasis determined by reverse transcriptase-polymease chain reaction显示文摘 | Noura S Ohue M Seki Y | 2008 | Oncol Rep2008，20，4： | 1 |
| 18 | Expression of second class of KIP protein p57KIP2 in human coloreetal carcinoma显示文摘 | Noura S Yamamoto H Sekimoto M | 2001 | Int J Oncol2001，19，1： | 1 |
| 19 | Immunohistochenical assessment of localization and frequency of micrometastases in lymph nodes of colorectal cancer显示文摘 | Noura S Yamamoto H Miyake Y | 2002 | Clin Cancer Res2002，8，3： | 1 |
| 20 | Clinical signficance of micrometastasis to lymph nodes in gastrointestinal tract cancers显示文摘 | Yamoto H Miyake Y Noura S | 2001 | Gan To Kagaku Ryoho2001，28，6： | 1 |
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