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| 1 | Relevance of MUC1 mucin variable number of tandem repeats polymorphism in H pylori adhesion to gastric epithelial cells显示文摘AIM:To evaluate the influence of MUC1 mucin variable number of tandem repeats (VNTR) variability on H pylori adhesion to gastric cells. METHODS: Enzyme linked immunosorbent assay (ELISA)-based adhesion assays were performed to measure the adhesion of different H pylori strains (HP26695 and HPTx30a) to gastric carcinoma cell lines (GP202 and MKN45) and GP202 clones expressing recombinant MUC1 with different VNTR lengths. RESULTS: Evaluation of adhesion results shows that H pylori pathogenic strain HP26695 has a significantly higher (P < 0.05) adhesion to all the cell lines and clones tested, when compared to the non-pathogenic strain HPTx30a. Bacteria showed a significantly higher (P < 0.05) adhesion to the GP202 cell line, when compared to the MKN45 cell line. Furthermore, both strains showed a significantly higher (P < 0.05) adhesion to GP202 clones with larger MUC1 VNTR domains. CONCLUSION: This work shows that MUC1 mucin variability conditions H pylori binding to gastric cells. The extent of bacterial adhesion depends on the size of theMUC1 VNTR domain. The adhesion is further dependent on bacterial pathogenicity and the gastric cell line. MUC1 mucin variability may contribute to determine H pylori colonization of the gastric mucosa. | Natália R Costa Nuno Mendes Nuno T Marcos Celso A Reis Thomas Caffrey Michael A Hollingsworth Filipe Santos-Silva | 2008 | World Journal of Gastroenterology2008，14，9： | 4 |
| 2 | Lymphocyte subsets in alcoholic liver disease显示文摘AIM:To compare lymphocyte subsets between healthy controls and alcoholics with liver disease.METHODS:The patient cohort for this study included individuals who were suspected to have alcoholic liver disease(ALD) and who had undergone liver biopsy(for disease grading and staging,doubts about diagnosis,or concurrent liver disease;n = 56).Normal controls included patients who were admitted for elective cholecystectomy due to non-complicated gallstones(n = 27).Formalin-fixed,paraffin-embedded liver biopsy specimens were sectioned and stained with hematoxylin and eosin and Perls' Prussian blue.The non-alcoholic steatohepatitis score was used to assess markers of ALD.Lymphocyte population subsets were determined by flow cytometry.T lymphocytes were identified(CD3+),and then further subdivided into CD4+ or CD8+ populations.B lymphocytes(CD19+) and natural killer(NK) cell numbers were also measured.In addition to assessing lymphocyte subpopulation differences between ALD patients and controls,we also compared subsets of alcoholic patients without cirrhosis or abstinent cirrhotic patients to normal controls.RESULTS:The patient cohort primarily consisted of older men.Active alcoholism was present in 66.1%.Reported average daily alcohol intake was 164.9 g and the average lifetime cumulative intake was 2211.6 kg.Cirrhosis was present in 39.3% of the patients and 66.1% had significant fibrosis(perisinusoidal and portal/periportal fibrosis,bridging fibrosis,or cirrhosis) in their liver samples.The average Mayo end-stage liver disease score was 7.6.No hereditary hemochromatosis genotypes were found.ALD patients(n = 56) presented with significant lymphopenia(1.5 × 109/L ± 0.5 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P < 0.0001),due to a decrease in all lymphocyte subpopulations,except for NK lymphocytes:CD3+(1013.0 ± 406.2/mm3 vs 1523.0 ± 364.6/mm3,P < 0.0001),CD4+(713.5 ± 284.7/mm3 vs 992.4 ± 274.7/mm3,P < 0.0001),CD8+(262.3 ± 140.4/mm3 vs 478.9 ± 164.6/mm3,P < 0.0001),and CD19+(120.6 ± 76.1/mm3 vs 264.6 ± 88.0/mm3,P < 0.0001).CD8+ lymphocytes suffered the greatest reduction,as evidenced by an increase in the CD4+/CD8+ ratio(3.1 ± 1.3 vs 2.3 ± 0.9,P = 0.013).This ratio was associated with the stage of fibrosis on liver biopsy(rs = 0.342,P = 0.01) and with Child-Pugh score(rs = 0.482,P = 0.02).The number of CD8+ lymphocytes also had a positive association with serum ferritin levels(rs = 0.345,P = 0.009).Considering only patients with active alcoholism but not cirrhosis(n = 27),we found similar reductions in total lymphocyte counts(1.8 × 109/L ± 0.3 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P = 0.018),and in populations of CD3+(1164.7 ± 376.6/mm3 vs 1523.0 ± 364.6/mm3,P = 0.001),CD4+(759.8 ± 265.0/mm3 vs 992.4 ± 274.7/mm3,P = 0.003),CD8+(330.9 ± 156.3/mm3 vs 478.9 ± 164.6/mm3,P = 0.002),and CD19+(108.8 ± 64.2/mm3 vs 264.6 ± 88.0/mm3,P < 0.0001).In these patients,the CD4+/CD8+ ratio and the number of NK lymphocytes was not significantly different,compared to controls.Comparing patients with liver cirrhosis but without active alcohol consumption(n = 11),we also found significant lymphopenia(1.3 × 109/L ± 0.6 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P < 0.0001) and decreases in populations of CD3+(945.5 ± 547.4/mm3 vs 1523.0 ± 364.6/mm3,P = 0.003),CD4+(745.2 ± 389.0/mm3 vs 992.4 ± 274.7/mm3,P = 0.032),CD8+(233.9 ± 120.0/mm3 vs 478.9 ± 164.6/mm3,P < 0.0001),and CD19+(150.8 ± 76.1/mm3 vs 264.6 ± 88.0/mm3,P = 0.001).The NK lymphocyte count was not significantly different,but,in this group,there was a significant increase in the CD4+/CD8+ ratio(3.5 ± 1.3 vs 2.3 ± 0.9,P = 0.01).CONCLUSION:All patient subsets presented with decreased lymphocyte counts,but only patients with advanced fibrosis presented with a significant increase in the CD4+/CD8+ ratio. | Luís Costa Matos Paulo Batista Nuno Monteiro Joo Ribeiro Maria A Cipriano Pedro Henriques Fernando Giro Armando Carvalho | 2013 | World Journal of Hepatology2013，5，2： | 4 |
| 3 | First report of CTX-M producing Escherichia coli , including the new ST2526, isolated from beef cattle and sheep in Portugal显示文摘 | Sónia Ramos Gilberto Igrejas Nuno Silva Daniela Jones-Dias José-Luís Capelo-Martinez Manuela Cani?a Patrícia Poeta | 2013 | Food Control2013，，1： | 1 |
| 4 | Vitamin D receptor: a new risk marker for clinical restenosis after percutaneous coronary intervention显示文摘 | Pascalle S Monraats Yue Fang Douwe Pons Nuno MM Pires Huibert AP Pols Aeilko H Zwinderman Moniek PM de Maat Pieter AF Doevendans Robbert J deWinter René A Tio Johannes Waltenberger Rune R Frants Paul HA Quax Arnoud van der Laarse Ernst E van der Wall Andr | 2010 | Expert Opinion on Therapeutic Targets2010，，： | 1 |
| 5 | Molecular epidemiology and antimicrobial susceptibility of extended- and broad-spectrum β-lactamase-producing Klebsiella pneumoniae isolated in Portugal显示文摘 | Nuno Mendon?a Eugénia Ferreira Deolinda Louro Manuela Cani?a | 2009 | International Journal of Antimicrobial Agents2009，，1： | 1 |
| 6 | Effect of carbon fibre reinforcement in the frictional behaviour of PEEK in a water lubricated environment显示文摘 | J Paulo Davim Nuno Marques A Monteiro Baptista | 2001 | Wear2001，251，： | 1 |
| 7 | Friedel-Crafts acylation of aromatic compoucds over arenesulfonic containing mesostructured SBA-15 materials显示文摘 | MELERO J A VAN GRIEKEN R MORALES G NUNO V | 2004 | Catal Comm2004，5，3： | 1 |
| 8 | Friending the Taxman:On the Use of Social Networking Services for Government eID in Europe显示文摘 | Martin A K Nuno N Andrade G | 2013 | Telecommunications Policy2013，37，： | 1 |
| 9 | Does combined dry land strength and aerobic training inhabit performance of young competitive swimmers显示文摘 | NUNO G DANIEL A M VICTOR M R | | 0，，09： | 1 |
| 10 | Ceria based solid catalysts for Fenton's depuration of phenolic wastewaters,biodegradability enhancement and toxicity removal显示文摘 | RUIC M NUNO A ROSA M Q-F | 2010 | Applied Catalysis B: Environmental2010，99，12： | 1 |
| 11 | Bacterial cel- lulose membranes as transdermal delivery systems for di- clofenac: In vitro dissolution and permeation studies 显示文摘 | Nuno H C S S Artur F R Isabel F A | 2014 | Carbohydr Polym2014，106，： | 1 |
| 12 | Occttnence of organic micro- contaminants in the wastewater treatment process:A mini review显示文摘 | Nuno R Alessandro C Arminda A | 2012 | Hazard Mater2012，239，： | 1 |
| 13 | A Simple Method for the absolute determination of uranium enrichment by high - resolution T spectrometry 显示文摘 | Koroba R O Nuno G A B | 2006 | Appl Radiat Isotopes2006，64，： | 1 |
| 14 | Triple Therapy with High- Dose Proton-Pump Inhibitor, Amoxicillin, and Doxycycline Is Useless for Helicobacter pylori Eradication: A Proof-of-Concept Study 显示文摘 | Nuno A Jose MR Maria MD | 2014 | Helicobacter2014，19，2： | 1 |
| 15 | Assessing the role of basic control measures, antivirals and vaccine in curtailing pandemic influenza: scenarios for the US, UK and the Netherlands显示文摘 | Nuno M Chowell G Gumel A B | 2007 | J R Soc Interface2007，4，14： | 1 |
| 16 | The role of sucrose in amino polymers synthesized by the strongly acid process显示文摘 | Nuno A Costa Pereira Joao Ferra Joao | 2013 | Journal of Adhesion Science And Technology2013，27，7： | 1 |
| 17 | Tools for mapping high-throughput sequencing data显示文摘 | Nuno A F Johan R Alvis B | 2012 | Bioinformaties2012，28，24： | 1 |
| 18 | Predictors of Tissue-Type Plasminogen Activator Nonresponders According to Location of Vessel Occlusion显示文摘 | Nuno Mendon?a David Rodriguez-Luna Marta Rubiera Sandra Boned-Riera Marc Ribo Jorge Pagola Socorro Pi?eiro Pilar Meler Jose Alvarez-Sabin Joan Montaner Carlos A. Molina | 2012 | Stroke2012，，2： | 1 |
| 19 | Assessing the role of basic control measures, antivirals and vaccine in curtailing pandemie influenza: scenarios for the US, UK and the Netherlands 显示文摘 | NUNO M CHOWELL G GUMEL A B | 2007 | J R Soe Interface2007，4，14： | 1 |
| 20 | Assess ing the role of basic contro 1 measures, an tivirals and vaccine in curtailing pandemic inf luenza: scenarios forth eU S, UK and the Netherland s显示文摘 | Nuno M Chow el l G Gum el A B | 2007 | J R Soc In terface2007，4，14： | 1 |
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