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| 1 | NDRG2 expression and mutation in human liver and pancreatic cancers显示文摘AIM: To investigate the expression of NDRG2 and mutation of the entire coding region of NDRG2 in human liver and pancreatic cancers, and to further discuss the possible causes of NDRG2 distinct expression patterns.METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was used to analyze the expression of NDRG2 mRNA in 37 fresh cancer specimens (including 8 cases of pancreatic cancer and 29 cases of liver cancer) and adjacent normal tissues collected from clinical operation. In addition,mutation analysis of the whole coding region of NDRG2 in these cancers was examined by polymerase chain reactionsingle strand conformational polymorphism (PCR-SSCP).RESULTS: Compared with adjacent normal tissues, the expression levels of NDRG2 mRNA in corresponding cancer tissues reduced significantly (pancreatic cancer: 0.680±0.112 vs 2.089±0.214, P<0.01) (liver cancer: 0.894±0.098 vs 1.345±0.177, P<0.05). Using PCR-SSCP, the mutation of the whole coding region of NDRG2 was not found in those cancer tissues where the expression of NDRG2 mRNA reduced markedly.CONCLUSION: NDRG2 gene might express differently between normal tissues and cancer tissues, and might play an important role in the development of pancreatic cancer and liver cancer. Low expression of NDRG2 might be unrelated to the mutation of coding region of NDRG2. | Xiao-LanHu Xin-PingLiu Shu-XinLin Yan-ChunDeng NaLiu XiaLi Li-BoYao | 2004 | World Journal of Gastroenterology2004，10，23： | 26 |
| 2 | Development of an oral DNA vaccine against MG7-Ag of gastric cancer using attenuated salmonella typhimurium as carrier显示文摘AIM: To develop an oral DNA vaccine against gastric cancer and evaluate its efficacy in mice.METHODS: The genes of the MG7-Ag mimotope and a universal Th epitope (Pan-DR epitope, PADRE) were included in the PCR primers. By PCR, the fusion gene of the two epitopes was amplified. The fusion gene was confirmed by sequencing and was then cloned into pcDNA3.1(+) plasmid. The pcDNA3.1 (+)-MG7/PADRE was used to transfect an attenuated Salrmonella typhimuriurm.C57BL/6 mice were orally immunized with 1x108 cfu Salrmonella transfectants. Salmonella harboring the empty pcDNA3.1(+) plasmid and phosphate buffer saline (PBS)were used as negative controls. At the 6th week, serum titer of MG7-Ag specific antibody was detected by ELtSA.At the 8th week cellular immunity was detected by an unprimed proliferation test of the spleenocytes by using a [3H]-thymidine incorporation assay. Ehrlich ascites carcinoma cells expressing MG7-Ag were used as a model in tumor challenge assay to evaluate the protective effect of the vaccine.RESULTS: Serum titer of antibody against MG7-Ag was significantly higher in mice immunized with the vaccine than that in control groups (0.841 vs 0.347, P<0.01; 0.841 vs 0.298,P<0.01), while in vitro unprimed proliferation assay of the spleenocytes showed no statistical difference between those three groups. Two weeks after tumor challenge, 2 in 7 immunized mice were tumor free, while all the mice in the control groups showed tumor formation.
CONCLUSION: Oral DNA vaccine against the MG7-Ag momitope of gastric cancer is immunogenic. It can induce significant humoral immunity against tumor in mice, and the vaccine has partially protective effects. | Quan-LiHan Fan-PingMeng NaLiu Dai-MingFan Chang-CurGuo JieDing Bo-RongPan Zhao-CaiYu | 2003 | World Journal of Gastroenterology2003，9，6： | 12 |
| 3 | Oral attenuated Salmonella typhimurium vaccine against MG7-Ag mimotope of gastric cancer显示文摘AIM: To develop an oral attenuated Salmonella typhimurium vaccine against gastric cancer and to evaluate its efficacy in mice.METHODS: A complementary sequence of Nco I site and a sequence coding for MG7-Ag mimotope were designed at the 5' terminus of forward primer. Using p1.2 Ⅱ-HBCAg plasmid as template, PCR was performed to get a fusion gene of the mimotope and a HBcAg gene. The fusion gene was then subcloned into the plasmid pYA3341complementary to Salmonella typhimurium X4550, and the recombinant plasmid was then transformed into attenuated Salmonella typhimurium X4550. Balb/c mice were orally immunized with the recombinant Salmonella typhimurium X4550. The mice were immunized every 2 wk to reinforce the immunity. At the 6th wk, serum titer of antibody was detected by ELISA, and at the 8th wk,cellular immunity was detected by 51Cr release test. Ehrlich ascites carcinoma cells expressing MG7-Ag were used in tumor challenge assay as a model to evaluate the protective effect of the vaccine.RESULTS: Serum titer of antibody against MG7-Ag was significantly higher in mice immunized with the vaccine than in control groups (0.9538±0.043 vs0.6531±0.018,P<0.01; 0.9538±0.043 vs0.6915±0.012, P<0.01), while in vitro 51Cr release assay of the splenocytes showed no statistical difference in the three groups. Two weeks after tumor challenge, 1 in 5 immunized mice was tumor free, while all the mice in the control group presented tumor.CONCLUSION: Oral attenuated Salmonella typhimurium vaccine against the MG7-Ag mimotope of gastric cancer is immunogenic. It can induce significant humoral immunity against tumors in mice, and has some protective effects. | Fan-PingMeng JieDing Zhao-CaiYu Quan-LiHan Chang-CunGuo NaLiu Dai-MingFan | 2005 | World Journal of Gastroenterology2005，11，12： | 6 |
| 4 | Effects of Aging on the Proliferation and Differentiation Capacity of Human Periodontal Ligament Stem Cells显示文摘这研究的目的是调查牙齿周围的系带干细胞( PDLSC )的增长,区别和 apoptosis 的目的源于不同年老的施主,并且通过手术从 24 被获得在 PDLSC.Methods 牙齿周围的系带纸巾的生物特征上评估老化的效果在 orthodontics 期间提取了人的前臼齿治疗。标本根据施主年龄被划分成三个组。组织 A:18-20 年,组 B:30-35 年,组 C:45-50 年。PDLSC 被孤立并且用由限制冲淡试金的一个 tissue-block-based enzymolytic 方法有教养。为三个试验性的组形成 PDLSC 的效率的殖民地是坚定的。联系老朽的牛乳糖(SA -- G ) 在三个组的表示用染色工作的牛乳糖被检验解决方案。PDLSC 的房间周期和 apoptosis 被流动 cytometry 检验。碱的磷酸酶(高山) 活动被高山染色评估。成骨的区别的表示联系了基因老牛相关的抄写 factor-2 ( Runx-2 ),骨胶原类型 1 ( co-1 ),并且 PDLSC 的高山被在组 A , B 和 C 形成 PDLSC 的效率的殖民地是的量的即时 RT-PCR.Results 检验36.67%,22.67%和9.33%,分别地它与施主年龄减少了( P < 0.05 )。SA -- 在组 A, B 和 C 的衰老的 PDLSC 的 G 表示是 4.14% , 16.39% , 50.38% ,分别地(P < 0.05 ) 。在 G2/S 的房间分阶段执行是 38.73% , 29.88% , 18.25%(P < 0.05 ) ,并且 apoptosis 率是 1.57% , 4.56% , 5.84%(P < 0.05 ) 在组 A, B 和 C 分别地。在三个组染色的高山随施主年龄的增加减少了,并且 Runx-2 , col-1 和高山的表示从组 A 逐渐地减少了组织 C (所有 P < 0.05 ),它显示当施主 aging.Conclusion 人 PDLSC 能成功地从不同年老的施主的牙齿周围的系带纸巾被孤立时, PDLSC 的 osteogenic 区别能力减少了。然而,当施主变老时,增长和 PDLSC 的 osteogenic 区别能力减少了。 | TingtingDu NaLiu BinGu YingLi YifangYuan WeiZhang TongZhang | 2017 | Chinese Medical Sciences Journal2017，32，2： | 3 |
| 5 | One‐Step Ionic‐Liquid‐Assisted Electrochemical Synthesis of Ionic‐Liquid‐Functionalized Graphene Sheets Directly from Graphite显示文摘 | NaLiu FangLuo HaoxiWu YinghuiLiu ChaoZhang JiChen | 2008 | Adv Funct Mater2008，，10： | 1 |
| 6 | One‐Step Ionic‐Liquid‐Assisted Electrochemical Synthesis of Ionic‐Liquid‐Functionalized Graphene Sheets Directly from Graphite显示文摘 | NaLiu FangLuo HaoxiWu YinghuiLiu ChaoZhang JiChen | 2008 | Adv Funct Mater2008，，10： | 1 |
| 7 | CHARACTERISTICS OF BENTONITE AND ITS SYNERGISTIC RETENTION EFFECT WITH CPAM ON WHEAT STRAW PULP显示文摘The various properties of bentonite samples with different sources and their synergistic retention effect with CPAM on wheat straw pulps were investigated. The investigated properties of bentonite included adsorptive capacity based on methylene blue, cation-exchange capacity, swelling volume, colloidal volume, particle size and charge properties. The results show that particle size is the most important properties of bentonite for its synergistic retention effect with CPAM. Using Wyoming type sodium bentonite without drying after modification can obtain the excellent furnishes retention. | NaLiu WenxiaLiu | 2004 | 天津科技大学学报2004，19，A01： | 0 |
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