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| 1 | Accelerated Amyloid Deposition in the Brains of Transgenic Mice Coexpressing Mutant Presenilin 1 and Amyloid Precursor Proteins显示文摘 | David R Borchelt Tamara Ratovitski Judy van Lare Michael K Lee Vicki Gonzales Nancy A Jenkins Neal G Copeland Donald L Price Sangram S Sisodia | 1997 | Neuron1997，，4： | 1 |
| 2 | Prevention and Control of Influenza with Vaccines Recommendations of the Advisory Committee on Immunization Practices (ACIP), 2010显示文摘 | Fiore Anthony E Uyeki Timothy M Broder Karen Finelli Lyn Euler Gary L Singleton James A Iskander John K Wortley Pascale M Shay David K Bresee Joseph S Cox Nancy J | 2010 | MMWR. Morbidity and Mortality Weekly Report2010，，： | 1 |
| 3 | Biological and immunological characterization of multiple GnRH in an opisthobranch mollusk Aplysia californica 显示文摘 | ZHANG L H NANCY L W NANCY M S | 2000 | General and Comparative Endocrinology2000，118，1： | 1 |
| 4 | Using the chlorite ion tocontrol nitrification显示文摘 | MICHAEL J M NANCY I L MARIE S P | 1999 | American Water Works Association Journal1999，91，10： | 1 |
| 5 | Bionanocomposites based on poly(3-caprolactone)-grafted cellulose nanocrystals by ring-opening polymerization显示文摘 | Youssef H Anne L G~ Nancy S | 2008 | J Mater Chem2008，18，41： | 1 |
| 6 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 7 | Air rage : Choice of law for inten- tional torts occurring in flight over international waters 显示文摘 | NANCY L F KIMBERLY A S | 1999 | Alba- ny Law Review1999，，9： | 1 |
| 8 | Market structure and innova- tion: a survey显示文摘 | MORTON I K NANCY L S | 1975 | Journal of Economic Literature1975，，13： | 1 |
| 9 | Controlling surface morphology of electrospun polystyrene fibers:effect of humidity and molecular weight in the electrospinning process显示文摘 | CHERYL L Casper JEAN S Stephens NANCY G Tassi | 2004 | Macromolecules2004，，37： | 1 |
| 10 | Controlling surface morphology of electrospun polystyrene fibers:Effect of humidity and molecular weight in the electrospinning process显示文摘 | Casper Cheryl L Stephens Jean S Tassi Nancy G | 2004 | Macromolecules2004，37，2： | 1 |
| 11 | Depression and cardiac risk: present status and future directions显示文摘 | NANCY F S FRANCOIS L | 2010 | Heart2010，96，3： | 1 |
| 12 | Neonatal manipulation of oxytocin influences female reproductive behavior and success显示文摘 | Cushing B S Levine K Nancy L C | 2005 | Hormones and Behavior2005，47，1： | 1 |
| 13 | Controlling surface morphology of electrospun polystyrene fobers: Effect of humidity and molecular weight in the electro- spinning process显示文摘 | Cheryl L C Jean S S Nancy G T | 2004 | Macromolecules2004，37，2： | 1 |
| 14 | Identification of theHPV-16 E6 protein from transformed mouse cells and humancervical carcinoma cell lines 显示文摘 | Elliot J A Nancy L H John T S | 1987 | The EMBO Journal1987，6，4： | 1 |
| 15 | Quality of life in immune thrombocytopenia following treatment显示文摘 | John D Grainger Nancy L Young Victor S Blanchette | 2013 | Archives of Disease in Childhood2013，22，11： | 1 |
| 16 | Comparison and verification of quantitative competitive reverse transcription polymerase chain reaction (QC-RTPCR) and real time显示文摘 | Yongbaek K Saad M G Nancy L S | 2002 | J Virol Methods2002，102，： | 1 |
| 17 | WHO classification of tumours of haematopoietic and lymphoid tissyes显示文摘 | STEVEN H S ELIAS C NANCY L H | 2008 | IARC2008，，： | 1 |
| 18 | Use of Double-stranded RNA Interference in Drosophila Cell Lines to Dissect Sigrlal Transduction Pathways显示文摘 | James C C Carolyn A W Nancy S L | 2000 | PNAs2000，97，： | 1 |
| 19 | Isogene specific oligo arrays reveal multifaceted changes in gene expression during grape berry (Vitis vinifera L.) development显示文摘 | Nancy Terrier David Glissant Jér?me Grimplet Fran?ois Barrieu Philippe Abbal Carole Couture Agnès Ageorges Rossitza Atanassova Céline Léon Jean-Pierre Renaudin Fabienne Dédaldéchamp Charles Romieu Serge Delrot Sa?d Hamdi | 2005 | Planta2005，，5： | 1 |
| 20 | Development and Psychometric Evaluation of the Pediatric Anesthesia Emergence Delirium Scale 显示文摘 | Nancy S Jerrold L | 2004 | Anesthesiology2004，100，5： | 1 |
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