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3篇 您的检索式:作者名="Nathan Washburn"
    题名 作者 年代 出处 被引量
1Cultural and Leadership Predictors of Corporate Social Responsibility Values of Top Management: A GLOBE Study of 15 Countries 显示文摘David A Waldman Mary Sully de Luque Nathan Washburn Robert J House 2006Journal of International Business Studies2006,,37:1
2Cultural and Leadership Predictors of Corporate Social Responsibility Values of Top Management:A GLOBE Study of 15 Countries显示文摘David A Waldman Mary Sully de Luque Nathan Washburn Robert J House 0,,:1
3Scalable and automated CRISPR-based strain engineering using droplet microfluidics显示文摘We present a droplet-based microfluidic system that enables CRISPR-based gene editing and high-throughput screening on a chip.The microfluidic device contains a 10x10 element array,and each element contains sets of electrodes for two electric field-actuated operations:electrowetting for merging droplets to mix reagents and electroporation for transformation.This device can perform up to 100 genetic modification reactions in parallel,providing a scalable platform for generating the large number of engineered strains required for the combinatorial optimization of genetic pathways and predictable bioengineering.We demonstrate the system's capabilities through the CRISPR-based engineering of two test cases:(1)disruption of the function of the enzyme galactokinase(galK)in£coli and(2)targeted engineering of the glutamine synthetase gene(glnA)and the blue-pigment synthetase gene(bpsA)to improve indigoidine production in£coli.Kosuke Iwai Maren Wehrs Megan Garber Jess Sustarich Lauren Washburn Zachary Costello Peter W.Kim David Ando William R.Gaillard Nathan J.Hillson Paul D.Adams Aindrila Mukhopadhyay Hector Garcia Martin Anup K.Singh 2022Microsystems & Nanoengineering2022,8,2:0
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