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| 1 | Quantitative determination of 1-deoxynojirimycin in mulberry leaves using liquid chromatography-tandem mass spectrometry显示文摘 | Nuengchamnong N Ingkaninan K Kaewruang W | | 0，，： | 1 |
| 2 | Antioxidant activities and phytochemical constituents of Antidesma thwaitesianum Müll. Arg. leaf extracts显示文摘OBJECTIVE: To investigate the antioxidant activities as well as phytochemical constituents of Antidesma thwaitesianum Müll. Arg. leaf extracts.METHODS: The leaves of A. thwaitesianum were extracted using three different methods: blending with distilled water, maceration with ethanol and decoction. The chemical antioxidant activity of the plant leaf extracts was evaluated using 2,2-diphenyl-1-picryhydrazyl(DPPH) radical and 2,2′-azinobis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt(ABTS+) radical scavenging assays, as well as the ferric reducing antioxidant power assay. Cellular antioxidant activity was determined by superoxide and nitric oxide scavenging assays. The cytotoxicity of the leaf extracts in RAW 264.7 and differentiated HL-60 cells was tested in parallel using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assays, respectively. The total phenolic and flavonoid contents were also assessed by spectrophotometric analysis. Phytochemical constituents of the most potent extract were investigated by liquid chromatography with an electrospray ionization quadrupole time-of-flight mass spectrometer(LC-ESI-QTOF-MS/MS).RESULTS: The ethanolic(ME) and decoction(LW) extracts of dried leaves had the highest chemical scavenging activity against DPPH and ABTS+ free radicals with half maximal effective concentration(EC50) values ranging from 3.54 to 6.44 μg/mL. ME and LW exerted moderate ferric reducing activity, with ferric reducing antioxidant power values of 847.41 and 941.26 mg Fe2+/g extract, respectively. Similarly, ME showed potent cellular scavenging activity against superoxide and nitric oxide radicals with EC50 values of 58.12 and 71.90 μg/mL, respectively. However, LW exhibited only strong nitric oxide scavenging activity with an EC50 value of 91.20 μg/mL. The cell viability of RAW 264.7 and HL-60 cells was greater than 70% in all tested concentrations of both extracts, thus confirming the absence of their cytotoxicity. ME and LW contained high total phenolic contents of 231.14 and 274.42 mg gallic acid equivalents per gram, respectively, as well as high total flavonoid contents of 18.82 and 22.17 mg quercetin equivalents per gram, respectively. LC-ESI-QTOFMS/MS analysis revealed the presence of 52 structurally characterized compounds in ME, 43 of whichwere tentatively identified. Hydroxycinnamic acids such as caffeic acid and its derivatives were the predominant phenolic compounds.CONCLUSION: This is the first report describing potent chemical and cellular antioxidant effects of the ethanolic leaf extract of A. thwaitesianum. The extract contained high total phenolic and flavonoid contents. LC-ESI-QTOF-MS/MS analysis further revealed an abundance of caffeic acid derivatives and flavonoids. These data support its potential use as dietary supplements in oxidative stress prevention. | Bhanuz Dechayont Arunporn Itharat Pathompong Phuaklee Jitpisute Chunthorng-Orn Thana Juckmeta Nuntika Prommee Nitra Nuengchamnong Pintusorn Hansakul | 2017 | Journal of Integrative Medicine2017，15，4： | 1 |
| 3 | Quantita- tive determination of l-deoxynojirimyein in mulberry leaves using liquid chromatography-tandem mass spectrometry 显示文摘 | Nuengchamnong N Ingkaninan K Kaewruang W | 2007 | J Phann Bi- omed Anal2007，44，4： | 1 |
| 4 | Quantitative determination of 1 -deoxynojirimycin in mulberry leaves using liquid chromatography -tandem mass spectrometry 显示文摘 | Nuengchamnong N Ingkaninan K Kaewruang W | 2007 | J Pharm Biomed Anal2007，44，4： | 1 |
| 5 | HPLC Coupled on-line to ESI-MS and a DPPH-based assay for the rapid identification of anti-oxidants in Butea superb 显示文摘 | Nuengchamnong N de Jong C F Bruyneel B | 2005 | Phytochem Anal2005，16，6： | 1 |
| 6 | Quantitative determination of1-deoxynojirimycin in mulberry leaves using liquid chromatography-tandem mass spectrometry显示文摘 | Nitra Nuengchamnong Kornkanok Ingkanian Wiroje Kaewru-ang | 2007 | J Phar Biom2007，44，： | 1 |
| 7 | Characterisation of phenolic antioxidants in aqueous extract of orthosiphon grandiflorus tea by LC-ESI-MS/MScoupled to DPPH assay显示文摘 | Nuengchamnong N Krittasilp K Ingkaninan K | 2011 | Food Chem2011，127，3： | 1 |
| 8 | Rapidscreening and identification of antioxidants in aqueous extracts ofhouttuynia cordata using LC-ESI-MS coupled with DPPH assay显示文摘 | NUENGCHAMNONG N KRITTASILP K INGKANINAN K | 2009 | Food Chemistry2009，117，4： | 1 |
| 9 | Rapid screening and identification of antioxidants in aqueous extracts of Houttuynia cordata using LC-ESI-MS cou- pled with DPPH assay显示文摘 | Nuengchamnong N Krittasilp K Ingkaninan K | 2009 | Food Chemistry2009，117，4： | 1 |
| 10 | Quantitative determination of 1 - deoxynojifimycin in mulberry leaves using liquid chromatography - tandem mass spectrometry 显示文摘 | Nuengchamnong N lngkaninan K Kaewrnang W | 2007 | J Pharmaceut Biomed2007，44，4： | 1 |
| 11 | On- line HPLC-MS-DPPH assay for the analysis of phenolic antioxidant compounds in fruit wine: Antidesma thwaitesianum Muell显示文摘 | Nitra Nuengchamnong Kornkanok Ingkaninan | 2010 | Food Chemistry2010，118，： | 1 |
| 12 | Quantitative determination of 1-deoxynojirimycin in mulberry leaves using liquid chromatography-tandem mass spectrometry显示文摘 | NUENGCHAMNONG N 1NGKANINAN K KAEWRUANG W | 2007 | Journal of Pharmaceutical and Biomedical Analysis2007，44，4： | 1 |
| 13 | Quantitative determination of 1-deoxynojirimycin in mulberry leaves using liquid chromatography-tandem mass spectrometry显示文摘 | NUENGCHAMNONG N INGKANINAN K KAEWRUANG W | 2007 | Journal of Pharmaceutical and Biomedical Analysis2007，44，4： | 1 |
| 14 | HPLC coupled on-line to ESI-MS and a DPPH-based assay for the rapid identification of anti-oxidants in Butea superba显示文摘 | de Jong CF Bruyneel B | 2005 | Phytochem Anal2005，16，6： | 1 |
| 15 | Quantitative determination of 1-deoxynojirimycin in mulberry leaves using liquid chromatography-tandem mass spectrometry显示文摘 | NUENGCHAMNONG N INGKANINAN K KAEWRUANG W | 2007 | Journal of Pharmaceutical and Biomedical Analysis2007，44，4： | 1 |
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