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| 1 | ^(99m)TC-Methylene diphosphonate uptake at injury site correlates with osteoblast differentiation and mineralization during bone healing in mice显示文摘99m Tc-Methylene diphosphonate(99m Tc-MDP) is widely used in clinical settings to detect bone abnormalities.However, the mechanism of99 m Tc-MDP uptake in bone is not well elucidated. In this study, we utilized a mouse tibia injury model, single-photon emission computed tomography(gamma scintigraphy or SPECT),ex vivo micro-computed tomography, and histology to monitor99 m Tc-MDP uptake in injury sites during skeletal healing. In an ex vivo culture system, calvarial cells were differentiated into osteoblasts with osteogenic medium, pulsed with99 m Tc-MDP at different time points, and quantitated for99 m Tc-MDP uptake with a gamma counter. We demonstrated that99 m Tc-MDP uptake in the injury sites corresponded to osteoblast generation in those sites throughout the healing process. The99 m Tc-MDP uptake within the injury sites peaked on day 7 post-injury, while the injury sites were occupied by mature osteoblasts also starting from day 7.99 m Tc-MDP uptake started to decrease 14 days post-surgery, when we observed the highest level of bony tissue in the injury sites. We also found that99 m Tc-MDP uptake was associated with osteoblast maturation and mineralization in vitro. This study provides direct and biological evidence for99 m Tc-MDP uptake in osteoblasts during bone healing in vivo and in vitro. | Zhendong A Zhong Anderson Peck Shihong Li Jeff Van Oss John Snider Casey J Droscha Tingtung A Chang Bart O Williams | 2015 | Bone Research2015，3，2： | 2 |
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| 8 | The interfacial tensions with water and the Lewis acid-base surface tension parameters of polar organic liquids derived from their aqueous solubilities 显示文摘 | Van Oss C J Wu W Docoslis A | 2001 | Colloids and Surfaces B : Biointefaces2001，20，1： | 1 |
| 9 | Added mass and damping in fluid-structure interaction 显示文摘 | Conca C Osses A Planchard J | 1997 | Computer Methods in Applied Mechanics and Engineering1997，146，34： | 1 |
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