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| 1 | Dynamic tracking of stem cells in an acute liver failure model显示文摘AIM:To investigate a dual labeling technique,which would enable real-time monitoring of transplanted embryonic stem cell(ESC) kinetics,as well as long-term tracking.METHODS:Liver damage was induced in C57/BL6 male mice(n = 40) by acetaminophen(APAP) 300 mg/kg administered intraperitoneally.Green fluorescence protein(GFP) positive C57/BL6 mouse ESCs were stained with the near-infrared fluorescent lipophilic tracer 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide(DiR) immediately before transplantationinto the spleen.Each of the animals in the cell therapy group(n = 20) received 5 × 10 6 ESCs 4 h following treatment with APAP.The control group(n = 20) received the vehicle only.The distribution and dynamics of the cells were monitored in real-time with the IVIS Lumina-2 at 30 min post transplantation,then at 3,12,24,48 and 72 h,and after one and 2 wk.Immunohistochemical examination of liver tissue was used to identify expression of GFP and albumin.Plasma alanine aminotransferase(ALT) was measured as an indication of liver damage.RESULTS:DiR-stained ESCs were easily tracked with the IVIS using the indocyanine green filter due to its high background passband with minimal background autofluorescence.The transplanted cells were confined inside the spleen at 30 min post-transplantation,gradually moved into the splenic vein,and were detectable in parts of the liver at the 3 h time-point.Within 24 h of transplantation,homing of almost 90% of cells was confirmed in the liver.On day three,however,the DiR signal started to fade out,and ex vivo IVIS imaging of different organs allowed signal detection at time-points when the signal could not be detected by in vivo imaging,and confirmed that the highest photon emission was in the liver(P < 0.0001).At 2 wk,the DiRsignal was no longer detectable in vivo ;however,immunohistochemistry analysis of constitutively-expressed GFP was used to provide an insight into the distribution of the cells.GFP +ve cells were detected in tissue sections resembling hepatocytes and were dispersed throughout the hepatic parenchyma,with the presence of a larger number of GFP +ve cells incorporated within the sinusoidal endothelial lining.Very faint albumin expression was detected in the transplanted GFP +ve cells at 72 h;however at 2 wk,few cells that were positive for GFP were also strongly positive for albumin.There was a significant improvement in serum levels of ALT,albumin and bilirubin in both groups at 2 wk when compared with the 72 h time-point.In the cell therapy group,serum ALT was significantly(P = 0.016) lower and albumin(P = 0.009) was significantly higher when compared with the control group at the 2 wk time-point;however there was no difference in mortality between the two groups.CONCLUSION:Dual labeling is an easy to use and cheap method for longitudinal monitoring of distribution,survival and engraftment of transplanted cells,and could be used for cell therapy models. | Tarek Ezzat Dipok Kumar Dhar Massimo Malago Steven WM Olde Damink | 2012 | World Journal of Gastroenterology2012，18，6： | 12 |
| 2 | Mapping protein post- translational modifications with mass spectrometry显示文摘 | Witze ES Old WM Resing KA | 2007 | Nature Methods2007，4，10： | 1 |
| 3 | The non-coding B2 RNA binds to the DNA cleft and active-site region of RNA polymerase Ⅱ显示文摘 | Ponicsan SL Houel S Old WM | 2013 | J Mol Biol2013，425，19： | 1 |
| 4 | Comparison of label-free methods for quantifying human proteins by shotgun proteomics显示文摘 | Old WM Meyer-Arendt K Aveline-Wolf L | | 0，，10： | 1 |
| 5 | Functional proteomics identifies targets of phosphorylation by B-Raf signaling in melanoma 显示文摘 | Old WM Shabb JB Houel S | 2009 | Mol Cell2009，34，1： | 1 |
| 6 | Noninvasive prenatal diagnosis of aneuploidy using cell-free nucleic acids in maternal blood : promiscs and unanswered questions 显示文摘 | Puszyk WM Crea F Old RW | 2008 | Prenat Diagn2008，28，1： | 1 |
| 7 | Noninvasive prenatal diagnosis of aneuploidy using cell-free nucleic acids in maternal blood:promises and unanswered questions显示文摘 | Puszyk WM Crea F Old RW | 2008 | Prenat Diagn2008，28，1： | 1 |
| 8 | Analysis of membrane proteins from human chronic myelogenous leukemia cells: comparison of extraction methods for multidimensional LC- MS/MS显示文摘 | Ruth MC Old WM Emrick MA | 2006 | J Proteorne Res2006，5，3： | 1 |
| 9 | Mapping protein post-translational modifications with mass spectrometry显示文摘 | Witze ES Old WM Resing KA | 2007 | Nat Methods2007，4，10： | 1 |
| 10 | Unequal representation of different unique genomic DNA sequences in the cell-free plasma DNA of indi- vidual donors 显示文摘 | Puszyk WM Crea F Old RW | 2009 | Clinbiochem2009，42，78： | 1 |
| 11 | Noninvasive prenatal diagnosis of aneuploidy using cell - free nucleic acids in maternal blood : promises and unanswered questions 显示文摘 | Puszyk WM Crea F Old RW | 2008 | Prenat Diagn2008，28，1： | 1 |
| 12 | Unequal representation of different unique genomic DNA sequences in the cell-free plasma DNA of individual donors显示文摘 | Puszyk WM Crea F Old RW | 2009 | Clin Biochem2009，47，78： | 1 |
| 13 | Fixation methods for electron microscopy of human and other liver显示文摘For an electron microscopic study of the liver,expertise and complicated,time-consuming processing of hepatic tissues and cells is needed.The interpretation of electron microscopy(EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation,embedding,sectioning,contrast staining and microscopic imaging.Hence,the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue,for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects. | Eddie Wisse Filip Braet Hans Duimel Celien Vreuls Ger Koek Steven WM Olde Damink Maartje AJ van den Broek Bart De Geest Cees HC Dejong Chise Tateno Peter Frederik | 2010 | World Journal of Gastroenterology2010，16，23： | 1 |
| 14 | Ophthalmic acid as a read-out for hepatic glutathione metabolism in humans显示文摘Background and Aim:Animal studies indicated that systemic ophthalmic acid(OPH)is a biomarker for hepatic glutathione(GSH)homeostasis,an important determinant of liver function.We aimed to clarify whether OPH levels can be used as a read-out for hepatic GSH homeostasis after paracetamol(APAP)challenges during pylorus-preserving pancreaticoduodenectomy(PPPD)or partial hepatectomy(PH).Methods:Nineteen patients undergoing PPPD(n=7,control group)or PH(n=12)were included.APAP(1000 mg)was administered intravenously before resection(first challenge),and six and twelve hours later,with sequential blood sampling during this period.Arterial,hepatic and portal venous blood samples and liver biopsies were taken on three occasions during the first APAP challenge.Plasma and hepatic OPH and GSH levels were quantified,and venous-arterial differences were calculated to study hepatic release.Results:Systemic GSH levels decreased during the course of the APAP challenge in both surgical groups,without notable change in OPH levels.Hepatic GSH and OPH content was not affected within^3 hours after administration of the first APAP dose in patients undergoing PPPD or PH.In this period,net release of OPH by the liver was observed only in patients undergoing PPPD.Conclusions:The drop in circulating GSH levels following APAP administrations,did not result in an increase in plasma OPH in both patients with an intact liver and in those undergoing liver resection.Hepatic content of GSH and OPH was not affected during the first APAP dose.It is uncertain whether hepatic GSH homeostasis was sufficiently challenged in the present study.Relevance for patients:In the present study,plasma OPH seemed not useful as a marker for GSH depletion because APAP administration during liver surgery did not lead to(immediate)GSH depletion or increased OPH levels.Based on stable levels of hepatic GSH,OPH and thiyl radicals during surgery,standard APAP administration seems to be safe in a postoperative care program with regards to GSH homeostasis in this specific population.However,no general statements can be made on the basis of the current experiment,since GSH homeostasis and susceptibility to xenobiotic toxicity are influenced by several metabolic and genetic factors. | Kim MC van Mierlo Simon AWG Dello Mechteld C de Jong Hans MH van Eijk Theo M de Kok Jacob J Briedé Frank G Schaap Steven WM Olde Damink Cornelius HC Dejong | 2017 | Journal of Clinical & Translational Research2017，3，4： | 0 |
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