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| 1 | Cyclosporine versus tacrolimus in patients with HCV infection after liver transplantation:Effects on virus replication and recurrent hepatitis显示文摘瞄准:决定 calcineurin 禁止者, cyclosporine 和 tacrolimus 的效果,在丙肝上,在病人的周期性的丙肝的病毒(HCV ) 复制和活动张贴肝移植。方法:在我们的中心为在 1999 和 2003 之间的联系 HCV 的肝肝硬化收到了肝移植的 107 个病人的一个队的数据回顾地被分析。浆液 HCV-RNA 的水平和周期性的肝炎的活动在作为主要抑制免疫力的代理人和没在移植以后在开始的 12 瞬间以内导致胆汁的复杂并发症的不那样类似的抑制免疫力的政体收到了 cyclosporine 或 tacrolimus 的 47 个病人之间被比较。结果:HCV-RNA 在移植以后在 3 瞬间以内增加了,但是 cyclosporine 组和 tacrolimus 组之间的差别是不足道的(在 12 瞬间的 P=0.49 ) 。另外,由浆液 transaminases 决定了的周期性的肝炎并且门发炎和纤维变性的组织学的分级没在 12 瞬间(P=0.34 ) 以后显示出有效差量。结论:一个主要抑制免疫力的代理人不在肝移植以后在感染 HCV 的病人影响周期性的肝炎的正式就职或严厉的 Cyclosporine 或 tacrolimus。 | Philip Hilgard Alisan Kahraman Nils Lehmann Cornelia Seltmann Susanne Beckebaum R Stefan Ross Hideo A Baba Massimo Malago Christoph E Broelsch Guido Gerken | 2006 | World Journal of Gastroenterology2006，12，5： | 235 |
| 2 | Diagnosis of biliary strictures after liver transplantation:Which is the best tool?显示文摘AIM: To evaluate the diagnostic value of different indirect methods like biochemical parameters, ultrasound (US) analysis, CT-scan and MRI/MRCP in comparison with endoscopic retrograde cholangiography (ERC), for diagnosis of biliary complications after liver transplantation. METHODS: In 75 patients after liver transplantation, who received ERC due to suspected biliary complications, the result of the cholangiography was compared to the results of indirect imaging methods performed prior to ERC. The cholangiography showed no biliary stenosis (NoST) in 25 patients, AST in 27 and ITBL in 23 patients. RESULTS: Biliary congestion as a result of AST was detected with a sensitivity of 68.4% in US analysis (specificity 91%), of 71% in MRI (specificity 25%) and of 40% in CT (specificity 57.1%). In ITBL, biliary congestion was detected with a sensitivity of 58.8% in the US, 88.9%in MRI and of 83.3% in CT. However, as anastomotic or ischemic stenoses were the underlying cause of biliary congestion, the sensitivity of detection was very low. InMRI detected the dominant stenosis at a correct localization in 22% and CT in 10%, while US failed completely. The biochemical parameters, showed no significant difference in bilirubin (median 5.7; 4,1; 2.5 mg/dL), alkaline phosphatase (median 360; 339; 527 U/L) or gamma glutamyl transferase (median 277; 220; 239 U/L) levels between NoST, AST and ITBL.CONCLUSION: Our data confirm that indirect imaging methods to date cannot replace direct cholangiography for diagnosis of post transplant biliary stenoses. However MRI may have the potential to complement or precede imaging by cholangiography. Optimized MRCP-processing might further improve the diagnostic impact of this method. | Thomas Zoepf Evelyn J. Maldonado-Lopez Philip Hilgard Alexander Dech■ne Massimo Malago Christoph E. Broelsch Joerg Schlaak Guido Gerken | 2005 | World Journal of Gastroenterology2005，11，19： | 32 |
| 3 | Dynamic tracking of stem cells in an acute liver failure model显示文摘AIM:To investigate a dual labeling technique,which would enable real-time monitoring of transplanted embryonic stem cell(ESC) kinetics,as well as long-term tracking.METHODS:Liver damage was induced in C57/BL6 male mice(n = 40) by acetaminophen(APAP) 300 mg/kg administered intraperitoneally.Green fluorescence protein(GFP) positive C57/BL6 mouse ESCs were stained with the near-infrared fluorescent lipophilic tracer 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide(DiR) immediately before transplantationinto the spleen.Each of the animals in the cell therapy group(n = 20) received 5 × 10 6 ESCs 4 h following treatment with APAP.The control group(n = 20) received the vehicle only.The distribution and dynamics of the cells were monitored in real-time with the IVIS Lumina-2 at 30 min post transplantation,then at 3,12,24,48 and 72 h,and after one and 2 wk.Immunohistochemical examination of liver tissue was used to identify expression of GFP and albumin.Plasma alanine aminotransferase(ALT) was measured as an indication of liver damage.RESULTS:DiR-stained ESCs were easily tracked with the IVIS using the indocyanine green filter due to its high background passband with minimal background autofluorescence.The transplanted cells were confined inside the spleen at 30 min post-transplantation,gradually moved into the splenic vein,and were detectable in parts of the liver at the 3 h time-point.Within 24 h of transplantation,homing of almost 90% of cells was confirmed in the liver.On day three,however,the DiR signal started to fade out,and ex vivo IVIS imaging of different organs allowed signal detection at time-points when the signal could not be detected by in vivo imaging,and confirmed that the highest photon emission was in the liver(P < 0.0001).At 2 wk,the DiRsignal was no longer detectable in vivo ;however,immunohistochemistry analysis of constitutively-expressed GFP was used to provide an insight into the distribution of the cells.GFP +ve cells were detected in tissue sections resembling hepatocytes and were dispersed throughout the hepatic parenchyma,with the presence of a larger number of GFP +ve cells incorporated within the sinusoidal endothelial lining.Very faint albumin expression was detected in the transplanted GFP +ve cells at 72 h;however at 2 wk,few cells that were positive for GFP were also strongly positive for albumin.There was a significant improvement in serum levels of ALT,albumin and bilirubin in both groups at 2 wk when compared with the 72 h time-point.In the cell therapy group,serum ALT was significantly(P = 0.016) lower and albumin(P = 0.009) was significantly higher when compared with the control group at the 2 wk time-point;however there was no difference in mortality between the two groups.CONCLUSION:Dual labeling is an easy to use and cheap method for longitudinal monitoring of distribution,survival and engraftment of transplanted cells,and could be used for cell therapy models. | Tarek Ezzat Dipok Kumar Dhar Massimo Malago Steven WM Olde Damink | 2012 | World Journal of Gastroenterology2012，18，6： | 12 |
| 4 | Multidetector computed tomographic cholangiography in the evaluation of potential living liver donors显示文摘 | Tobias Schroeder Massimo Malago J?rg F. Debatin Giuliano Testa Silvio Nadalin Christoph E. Broelsch Stefan G. Ruehm | 2002 | Transplantation2002，，12： | 1 |
| 5 | Major bile duct injuries after laparoscopic cholecystectomy: A tertiary center experience显示文摘 | Andrea Frilling M.D. Ph.D. Jun Li M.D. Frank Weber M.D. Nils Roman Frühauf M.D. Jennifer Engel Susanne Beckebaum M.D. Andreas Paul M.D. Thomas Z?pf M.D. Massimo Malago M.D. Ph.D. Christoph Erich Broelsch M.D. Ph.D | 2004 | Journal of Gastrointestinal Surgery2004，，6： | 1 |
| 6 | Endoscopic Therapy of Posttransplant Biliary Stenoses After Right-Sided Adult Living Donor Liver Transplantation显示文摘 | Thomas Zoepf Evelyn J. Maldonado–lopez Philip Hilgard Joerg Schlaak Massimo Malago Christoph E. Broelsch Ulrich Treichel Guido Gerken | 2005 | Clinical Gastroenterology and Hepatology2005，，11： | 1 |
| 7 | Balloon dilatation vs. balloon dilatation plus bile duct endoprostheses for treatment of anastomotic biliary strictures after liver transplantation显示文摘 | Thomas Zoepf Evelyn J. Maldonado‐Lopez Philip Hilgard Massimo Malago Christoph E. Broelsch Ulrich Treichel Guido Gerken | 2005 | Liver Transpl2005，，1： | 1 |
| 8 | Surgical Management of Segmental and Sectoral Bile Duct Injury After Laparoscopic Cholecystectomy: a Challenging Situation显示文摘 | Jun Li Andrea Frilling Silvio Nadalin Sonia Radunz Juergen Treckmann Hauke Lang Massimo Malago Christoph Erich Broelsch | 2010 | Journal of Gastrointestinal Surgery2010，，2： | 1 |
| 9 | Major bile duct injuries after laparoscopic cholecystectomy: A tertiary center experience显示文摘 | Andrea Frilling M.D. Ph.D. Jun Li M.D. Frank Weber M.D. Nils Roman Frühauf M.D. Jennifer Engel Susanne Beckebaum M.D. Andreas Paul M.D. Thomas Z?pf M.D. Massimo Malago M.D. Ph.D. Christoph Erich Broelsch M.D. Ph.D | 2004 | Journal of Gastrointestinal Surgery2004，，6： | 1 |
| 10 | Activation of the ERK and AKT signalling pathway predicts poor prognosis in hepatocellular carcinoma and ERK activation in cancer tissue is associated with hepatitis C virus infection显示文摘 | Klaus Juergen Schmitz Jeremias Wohlschlaeger Hauke Lang Georgios Charalambos Sotiropoulos Massimo Malago Karen Steveling Henning Reis Vito Rosario Cicinnati Kurt Werner Schmid Hideo Andreas Baba | 2007 | Journal of Hepatology2007，，1： | 1 |
| 11 | Major bile duct injuries after laparoscopic cholecystectomy: a tertiary center experience显示文摘 | Andrea Frilling Jun Li Frank Weber Nils Roman Frühauf Jennifer Engel Susanne Beckebaum Andreas Paul Thomas Z?pf Massimo Malago Christoph Erich Broelsch | 2004 | Journal of Gastrointestinal Surgery2004，，6： | 1 |
| 12 | Surgical Management of Segmental and Sectoral Bile Duct Injury After Laparoscopic Cholecystectomy: a Challenging Situation显示文摘 | Jun Li Andrea Frilling Silvio Nadalin Sonia Radunz Juergen Treckmann Hauke Lang Massimo Malago Christoph Erich Broelsch | 2010 | Journal of Gastrointestinal Surgery2010，，2： | 1 |
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