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| 1 | Localization of Seed Oil Body Proteins in Tobacco Protoplasts Reveals Specific Mechanisms of Protein Targeting to Leaf Lipid Droplets显示文摘Oleosin, caleosin and steroleosin are normally expressed in developing seed cells and are targeted to oil bodies. In the present work, the cDNA of each gene tagged with fluorescent proteins was transiently expressed into tobacco protoplasts and the fluorescent patterns observed by confocal laser scanning microscopy. Our results indicated clear differences in the endocellular localization of the three proteins. Oleosin and caleosin both share a common structure consisting of a central hydrophobic domain flanked by two hydrophilic domains and were correctly targeted to lipid droplets (LD), whereas steroleosin, characterized by an N-terminal oil body anchoring domain, was mainly retained in the endoplasmic reticulum (ER). Protoplast fractionation on sucrose gradients indicated that both oleosin and caleosingreen fluorescent protein (GFP) peaked at different fractions than where steroleosin-GFP or the ER marker binding immunoglobulin protein (BiP), were recovered. Chemical analysis confirmed the presence of triacylglycerols in one of the fractions where oleosin-GFP was recovered. Finally, only oleosin- and caleosin-GFP were able to reconstitute artificial oil bodies in the presence of triacylglycerols and phospholipids. Taken together, our results pointed out for the first time that leaf LDs can be separated by the ER and both oleosin or caleosin are selectively targeted due to the existence of selective mechanisms controlling protein association with these organelles. | Stefania De Domenico Stefania Bonsegna Marcello Salvatore Lenucci Palmiro Poltronieri Gian Pietro Di Sansebastiano Angelo Santino | 2011 | Journal of Integrative Plant Biology2011，53，11： | 1 |
| 2 | Evaluation of the Microbial Safety of Child Food of Animal Origin in Greece显示文摘 | Emmanouil Liandris Maria Gazouli Styliani Taka Margarita Andreadou Anna Vaiopoulou Nikolaos Tzimotoudis Ioannis Kasampalidis Dionysis Mpaseas George Fyliousis Palmiro Poltrionieri Nigel Cook John Ikonomopoulos | 2014 | Journal of Food Science2014，，3： | 1 |
| 3 | A filtration-based real-time PCR method for the quantitative detection of viable Salmonellaenterica and Listeria monocytogenes in food samples显示文摘 | OSCAR F D PALMIRO P SANTO M | 2009 | FoodMicrobiology2009，26，： | 1 |
| 4 | Age- and calorie restriction-related changes in rat brain mitochondrial DNA and TFAM binding显示文摘 | Anna Picca Flavio Fracasso Vito Pesce Palmiro Cantatore Anna-Maria Joseph Christiaan Leeuwenburgh Maria Nicola Gadaleta Angela Maria Serena Lezza | 2013 | AGE2013，，5： | 1 |
| 5 | The PGC-1α-dependent pathway of mitochondrial biogenesis is upregulated in type I endometrial cancer显示文摘 | Antonella Cormio Flora Guerra Gennaro Cormio Vito Pesce Flavio Fracasso Vera Loizzi Palmiro Cantatore Luigi Selvaggi Maria Nicola Gadaleta | 2009 | Biochemical and Biophysical Research Communications2009，，4： | 1 |
| 6 | Ocular ultrasound evaluation of optic nerve sheath diameter in military environments显示文摘In this letter to the Editor, we would like to comment on the article by Betcher et al., concerning the possibility of teaching military trainees to obtain accurate optic nerve sheath diameter measurements, using a brief didactic and a hands-on training session. In particular, this letter notes the importance of the measurement of optic nerve sheath diameter in detecting the eventual elevated intracranial pressure following traumatic brain injury, highlights several limitations in the use of B-scan for such a purpose and suggests a more accurate evaluation with the standardized A-scan. | Maddalena De Bernardo Livio Vitiello Palmiro Cornetta Nicola Rosa | 2019 | Military Medical Research2019，6，3： | 0 |
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