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| 1 | Cloned pigs derived from somatic cell nuclear transferembryos cultured in vitro atlow oxygen tension显示文摘Pig cloning has great potential to human xenotransplantation. The present study was designed to establish a more efficient system for producing cloned pigs by somatic cell nuclear transfer (SCNT). Our approach was as follows: SCNT embryos were reconstructed by using fetal fibroblasts of Chinese miniature pig as donors and in vitro matured oocytes of prepubertal gilts as recipients. Reconstructed em- bryos were induced by electrical fusion/activation and cultured in BSA-containing North Carolina State University 23 medium (NCSU-23) or Porcine Zygote Medium (PZM-3) at the gas condition of 5% CO2, 7% O2, 88% N2. A total of 230 cloned embryos were transferred to three surrogate sows, producing three piglets. One of them is apparently healthy. The clonal provenance of the piglet was indicated by its coat color and confirmed by DNA microsatellite analysis. These results indicate that the use of in vitro matured oocytes from prepubertal gilts as recipient, combined with cloned embryos cultured at low oxygen tension is an effective way to produce cloned pigs. | PAN Dengke ZHANG Yunhai SUN Xiuzhu ZHANG Jian LI Xuyang LI Yan GU Zhiliang DA Yunping WU Changxin LI Ning | 2006 | Chinese Science Bulletin2006，51，7： | 8 |
| 2 | An anther-specific chalcone synthase-like gene D5 related to rice pollen development显示文摘It was shown in a previous analysis that D5 gene from rice (Oryza sativa L.) was an anther-specific gene encoding a chalcone synthase-related protein. In this study, D5 gene was found specifically expressed in tapetum cells as well as in the peripheral cells of the vascular bundle of rice anthers by RNA in situ hybridization. In order to study its function, D5 was transformed into rice in both sense and antisense directions driven by a rice Actin 1 promoter. It has been observed that the pollen grains from the antisense D5 transgenic rice plants are abnormal, indicating that D5 plays a critical role in rice pollen development. | Honghong Zheng Lijia Qu Meihua Liu Yi Zhang Yunping Shen Junming Wei Naisui Pan Hongya Gu Zhangliang Chen | 2000 | Chinese Science Bulletin2000，45，21： | 2 |
| 3 | The Tribological Properties of the Hot-extrusion Nylon Coating in the Oil Well Environment显示文摘 | Mo Yimin Pan Yunping Wang Xiumei | 2003 | Journal of Wuhan University of Technology:Materials Science Edition2003，18，4： | 1 |
| 4 | Axial spondyloarthritis:synthetic magnetic resonance imaging in the detection of sacroiliac joint lesions显示文摘To the Editor:Axial spondyloarthritis(axSpA)is characterized by sacroiliac joint inflammation,spine inflammation,bone destruction,and osteogenesis.Ankylosis of the spine and/or hip joints may occur later,significantly affecting the patient’s quality of life.As the earliest and most common imaging finding of axSpA,sacroiliitis can be divided into early active and late structural lesions.Magnetic resonance imaging(MRI)not only detects active inflammatory changes that cannot be found in computed tomography(CT)or X-rays but also has a similar sensitivity to CT in detecting structural lesions. | Ke Zhang Jing Zheng Jielin Pan Yunping Jiang Yingying Zhan Wenjuan Li Hua Zhang Guobin Hong | 2022 | Chinese Medical Journal2022，135，21： | 0 |
| 5 | Seasonal influence on the development of parthenotes and cloned embryos in pigs显示文摘Seasonal influence on the developmental ability of porcine embryos was studied by comparisons of the number of cumulus-oocytes-complexes (COCs) per ovary pair and meiotic maturation ability of oocytes in different seasons; and by the observations on developmental competence of the parthenogenetic oocytes and cloned embryos collected from different seasons. We found that the number of COCs per ovary pairs was significantly higher in spring compared to the other seasons (P<0.05). However, no significant difference was found in the rate of oocytes progressing to MⅡ in four seasons (P<0.05). In addition, the rate of blastocyst formation of parthenogenetic embryos in summer was obviously declined compared to the other seasons (P<0.05), and an increased blastocyst rate of cloned porcine embryos was found in spring compared to autumn and winter (P<0.05). The results suggest that there should be a seasonal influence on the developmental competence of porcine embryos. | Zhang Kun Zhang Yunhai Pan Dengke Ma Yufang Wei Hengxi Zhang Jian Li Xuyang Wang Shaohua Li Yan Dai Yunping Li Ning | 2007 | Progress in Natural Science:Materials International2007，17，10： | 0 |
| 6 | Whole-exome mutational landscape of neuroendocrine carcinomas of the gallbladder显示文摘Neuroendocrine carcinoma(NEC)of the gallbladder(GB-NEC)is a rare but extremely malignant subtype of gallbladder cancer(GBC).The genetic and molecular signatures of GB-NEC are poorly understood;thus,molecular targeting is currently unavailable.Inthe present study,we applied whole-exome sequencing(WES)technology to detect gene mutations and predicted somatic singlenucleotide variants(SNVs)in 15 cases of GB-NEC and 22 cases of general GBC.in 15 GB-NECs,the C>T mutation was predominantamong the 6 types of SNVs.TP53 showed the highest mutation frequency(73%,11/15).Compared with neuroendocrine carcinomasof other organs,signifcantly mutated genes(SMGs)in GB-NECs were more similar to those in pulmonary large-cell euroendocrinecarcinomas(LCNECs),with drver roles for TP53 and RB1.Iin the COSMIC database of cancer-related genes,211 genes were mutated.Strikingly,RB1(4/15,27%)and NAB2(3/15,20%)mutations were found specifically in GB-NECs;in contrast,mutations in 29 genes,including ERB82 and ERBB3,were identified exclusively in GBC.Mutations in RB1 and NAB2 were significanty related to downregulation of the RB1 and NAB2 proteins,respectively,according to immunohistochemical(IHC)data(p values=0.0453 and0.0303).Clinically actionable genes indicated 23 mutated genes,including ALK,BRCA1,and BRCA2.Iin addition,potential somaticSNVs predicted by ISowN and SomVarlUS constituted 6 primary coSMIC mutation signatures(1,3,30,6,7,and 13)in GB-NEC.Genes carrying somatic SNVs were enriched mainly in oncogenic signaling pathways involving the Notch,WNT,Hippo,and RTK-RASpathways.In summary,we have systematically identified the mutation landscape of GB-NEC,and these findings may providemechanistic insights into the specifc pathogenesis of this deadly disease. | Fatao Liu Yongsheng Li Dongjian Ying Shimei Qiu Yong He Maoian Li Yun Liu Yijian Zhang Qin Zhu Yunping Hu Liguo Liu Guoqiang Li Weihua Pan Wei Jin Jiasheng Mu Yang Cao Yingbin Liu | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 0 |
| 7 | Design and Realization of Numerical Control Ladder Diagram Edition Software显示文摘The thesis is directed by the idea of oriented-object. Considering the basic functions that NC system Ladder Diagram editor should provide,and through theoretical research and practice,the thesis developed a set of NC system Ladder Diagram editor which can form a Ladder Diagram editor based on vector plotting,intelligently compiling,simulation. This system uses the ladder diagram symbol to express operational order and use the chart symbol series-parallel connection and the position order to express the logical relations between the operational orders,divide the ladder diagram into four parts: the stave,the line,the row and the part,uses the standard order vessel list vessel of the standard template stack (STL) to save the data which involved in the design process. This system can write PLC program by ladder diagram language and is easy to use. The compilation and simulation for PLC diagram have been achieved. It greatly improves the work-efficiency. | ZHAO Haixin,MO Yimin,PAN Yunping (School of Mechanical and Electronical Engineering,Wuhan University of Technology, Wuhan 430070,China) | 2006 | 武汉理工大学学报2006，28，S1： | 0 |
| 8 | Physicochemical nature of iron oxidation in a damp atmospheric condition显示文摘The surface oxidation patterns of iron or low-carbon steels are critical to their life when serving in typical damp environments. An accurate determination of the oxidation pattern entails tracking the iron atoms oxidized at the iron/steel-moisture interface. Using a quantum chemistry-based force field that is capable of simulating chemical reactions, this paper studies the process of iron oxidation under a typical moist condition. The oxidation of iron surface was found to be highly thermodynamic and dependent on the availability of reactants. A triplex structure was formed at the end of a three-stage oxidation process to reduce the overall oxidation speed. The results from this study shed light on the atomistic mechanism of iron oxidation; therefore can be used to guide the protection of general ferrous-based iron/steel structures. | Tongyan PAN Yunping XI | 2011 | Acta Metallurgica Sinica(English Letters)2011，24，6： | 0 |
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