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| 1 | Inactivation of SACE_3446, a TetR family transcriptional regulator, stimulates erythromycin production in Saccharopolyspora erythraea显示文摘Erythromycin A is a widely used antibiotic produced by Saccharopolyspora erythraea;however,its biosynthetic cluster lacks a regulatory gene,limiting the yield enhancement via regulation engineering of S.erythraea.Herein,six TetR family transcriptional regulators(TFRs)belonging to three genomic context types were individually inactivated in S.erythraea A226,and one of them,SACE_3446,was proved to play a negative role in regulating erythromycin biosynthesis.EMSA and qRT-PCR analysis revealed that SACE_3446 covering intact N-terminal DNA binding domain specifically bound to the promoter regions of erythromycin biosynthetic gene eryAI,the resistant gene ermE and the adjacent gene SACE_3447(encoding a longchain fatty-acid CoA ligase),and repressed their transcription.Furthermore,we explored the interaction relationships of SACE_3446 and previously identified TFRs(SACE_3986 and SACE_7301)associated with erythromycin production.Given demonstrated relatively independent regulation mode of SACE_3446 and SACE_3986 in erythromycin biosynthesis,we individually and concomitantly inactivated them in an industrial S.erythraea WB.Compared with WB,the WBΔ3446 and WBΔ3446Δ3986 mutants respectively displayed 36%and 65%yield enhancement of erythromycin A,following significantly elevated transcription of eryAI and ermE.When cultured in a 5 L fermentor,erythromycin A ofWBΔ3446 and WBΔ3446Δ3986 successively reached 4095 mg/L and 4670 mg/L with 23%and 41%production improvement relative to WB.The strategy reported here will be useful to improve antibiotics production in other industrial actinomycete. | Hang Wu Yansheng Wang Li Yuan Yongrong Mao Weiwei Wang Lin Zhu Panpan Wu Chengzhang Fu Rolf Muller David T.Weaver Lixin Zhang Buchang Zhang | 2016 | Synthetic and Systems Biotechnology2016，1，1： | 7 |
| 2 | A novel selective autophagy receptor,CCDC50,delivers K63 polyubiquitination-activated RIG-I/MDA5 for degradation during viral infection显示文摘Autophagy is a con served process that delivers cytosolic substa nces to the lysosome for degradatio n,but its direct role in the regulation of antiviral inn ate immu nity remains poorly un derstood.Here,through high-throughput screeni ng,we discovered that CCDC50 functions as a previously unknown autophagy receptor that negatively regulates the type I interferon(IFN)signaling pathway initiated by RIG-I-like receptors(RLRs),the sensors for RNA viruses.The expression of CCDC50 is enhanced by viral infection,and CCDC50 specifically recognizes K63-polyubiquitinated RLRs,thus delivering the activated RIG-I/MDA5 for autophagic degradation.The associatio n of CCDC50 with phagophore membrane protei n LC3 is con firmed by crystal structure analysis.In contrast to other known autophagic cargo receptors that associate with either the LIR-docking site(LDS)or the UIMdocking site(UDS)of LC3,CCDC50 can bind to both LDS and UDS,representing a new type of cargo receptor.In mouse models with RNA virus infection,CCDC50 deficiency reduces the autophagic degradation of RIG-I/MDA5 and promotes type I IFN responses,resulting in enhaneed viral resistance and improved survival rates.These results reveal a new link between autophagy and antiviral innate immune resp on ses and provide additional in sights into the regulatory mecha nisms of RLR-mediated antiviral signaling. | Panpan Hou Kongxiang Yang Penghui Jia Lan Liu Yuxin Lin Zibo Li Jun Li Shuliang Chen Shuting Guo Ji'An Pan Junyu Wu Hong Peng Weijie Zeng Chunmei Li Yingfang Liu Deyin Guo | 2021 | Cell Research2021，31，1： | 5 |
| 3 | Flexible lead-free BFO-based dielectric capacitor with large energy density,superior thermal stability,and reliable bending endurance显示文摘As an essential energy-stored device,the inorganic dielectric film capacitor plays an irreplaceable role in high-energy pulse power technology area.In this work,propelled by the challenge of overcoming the bottlenecks of inflexibility and inferior energy storage density of the pure BiFeO3 films,the mica with high bendability and thermal stability is adopted as substrate,and the relaxor ferroelectric(Sr_(0.7)Bi_(0.2))TiO_(3) is introduced to form solid solution to introduce relaxor behavior.The subsequently fabricated 0.3Bi(Fe_(0.95)Mn_(0.05))O_(3)-0.7(Sr_(0.7)Bi_(0.2))TiO_(3)(BFMO-SBT)thin film capacitor exhibits a high recoverable energy storage density(W_(rec)=61 J cm^(-3))and a high efficiency(η=75%)combined with a fast discharging rate(23.5 μs)due to the large polarization difference(ΔP=59.4 μC cm^(-2)),high breakdown strength(E_(b)=3000 kV cm^(-1)),and the strong relaxor dispersion(γ=1.78).Of particular importance is the capacitor presents excellent stability of energy storage performance,including a wide working temperature window of -50-200℃,fatigue endurance of 108 cycles,and frequency range of 500 Hz-20 kHz.Furthermore,there are no obviously deteriorations on energy storage capability under various bending states and after 104 times of mechanical bending cycles.All these results indicate that BFMO-SBT on mica film capacitor has potential application in the future flexible electronics. | Changhong Yang Jin Qian Panpan Lv Haitao Wu Xiujuan Lin Kun Wang Jun Ouyang Shifeng Huang Xin Cheng Zhenxiang Cheng | 2020 | Journal of Materiomics2020，6，1： | 4 |
| 4 | Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment显示文摘 | Panpan Gao Daqing Mao Yi Luo Limei Wang Bingjie Xu Lin Xu | 2012 | Water Research2012，，7： | 3 |
| 5 | Autophagy receptor CCDC50 tunes the STING-mediated interferon response in viral infections and autoimmune diseases显示文摘DNA sensing and timely activation of interferon(IFN)-mediated innate immunity are crucial for the defense against DNA virus infections and the clearance of abnormal cells.However,overactivation of immune responses may lead to tissue damage and autoimmune diseases;therefore,these processes must be intricately regulated.STING is the key adaptor protein,which is activated by cyclic GMP-AMP,the second messenger derived from cGAS-mediated DNA sensing.Here,we report that CCDC50,a newly identified autophagy receptor,tunes STING-directed type I IFN signaling activity by delivering K63-polyubiquitinated STING to autolysosomes for degradation.Knockout of CCDC50 significantly increases herpes simplex virus 1(HSV-1)-or DNA ligand-induced production of type I IFN and proinflammatory cytokines.Ccdc50-deficient mice show increased production of IFN,decreased viral replication,reduced cell infiltration,and improved survival rates compared with their wild-type littermates when challenged with HSV-1.Remarkably,the expression of CCDC50 is downregulated in systemic lupus erythematosus(SLE),a chronic autoimmune disease.CCDC50 levels are negatively correlated with IFN signaling pathway activation and disease severity in human SLE patients.CCDC50 deficiency potentiates the cGAS-STING-mediated immune response triggered by SLE serum.Thus,our findings reveal the critical role of CCDC50 in the immune regulation of viral infections and autoimmune diseases and provide insights into the therapeutic implications of CCDC50 manipulation. | Panpan Hou Yuxin Lin Zibo Li Ruiqing Lu Yicheng Wang Tian Tian Penghui Jia Xi Zhang Liu Cao Zhongwei Zhou Chunmei Li Jieruo Gu Deyin Guo | 2021 | Cellular & Molecular Immunology2021，18，10： | 3 |
| 6 | Incidence of Macrosomia in Rural Areas—Henan Province,China,2013–2017显示文摘Introduction:Macrosomia has short-term and long-term adverse health effects and is thus an important public health concern.Recent decades have witnessed increasing incidence of macrosomia in many countries.Methods:The present study used a large population-based birth cohort study to depict incidence of macrosomia among live births in rural areas of Henan Province of China from 2013 to 2017.Results:Among the 1,262,916 births,82,353 were cases of macrosomia.The overall incidence of all types of macrosomia,of macrosomia with birth weight<4,500 g,and of macrosomia with birth weight≥4,500 g were 6.52%,5.30%,and 1.22%,respectively.From 2013 to 2017,the incidence of macrosomia decreased by 31.3%from 7.96%in 2013 to 5.47%in 2017(X^(2)_(trend)=946.96,P_(trend)<0.001).Male infants and infants≥42 gestational weeks had significantly higher incidence of macrosomia than that of female infants and infants<42 gestational weeks(P<0.001).Conclusion:Gestational weight control through nutrition management and physical activities during pregnancy are needed to reduce incidence of macrosomia. | Shiqi Lin Jian Chai Jiajia Li Xuejun Shang Lijun Pei Lifang Jiang Junxi Zhang Panpan Sun Wei Dong Yuhong Wang Dezhuan Zhou | 2021 | China CDC weekly2021，3，37： | 3 |
| 7 | Hierarchical porous MgBO_2(OH) microspheres: Hydrothermal synthesis,thermal decomposition, and application as adsorbents for Congo red removal显示文摘A facile eco-friendly hydrothermal route(180 °C, 12.0 h) has been developed for the first time to the uniform hierarchical porous MgBO_2(OH) microspheres without the aid of any organic additive, surfactant or template, by using the abundant MgCl_2·6 H_2 O, H_3BO_3 and NaOH as the raw materials. The as-obtained porous microspheres exhibit a specific surface area of 94.752 mg·g^(-1), pore volume of 0.814 cm3·g^(-1), and ca. 84.0% of which have a diameter of 2.25–3.40 μm. The thermal decomposition of the porous MgBO_2(OH) microspheres(650 °C,2.5 °C·min^(-1)) leads to the porous Mg_2B_2O_5 microspheres with well-retained morphology. When utilized as the adsorbents for the removal of CR from mimic waste water, the present porous MgBO_2(OH) microspheres exhibit satisfactory adsorption capacity, with the maximum adsorption capacity q^(-1) mof 309.1 mg·g, much higher than that derived from most of the referenced adsorbents. This opens a new window for the facile green hydrothermal synthesis of the hierarchical porous MgBO_2(OH) microspheres, and extends the potential application of the 3 D hierarchical porous metal borates as high-efficiency adsorbents for organic dyes removal. | Panpan Sun Liyuan Chen Lin Xu Wancheng Zhu | 2018 | Chinese Journal of Chemical Engineering2018，26，7： | 2 |
| 8 | Tunable Dynamic Black Phosphorus/Insulator/Si Heterojunction Direct-Current Generator Based on the Hot Electron Transport显示文摘Static heterojunction-based electronic devices have been widely applied because carrier dynamic processes between semiconductors can be designed through band gap engineering.Herein,we demonstrate a tunable direct-current generator based on the dynamic heterojunction,whose mechanism is based on breaking the symmetry of drift and diffusion currents and rebounding hot carrier transport in dynamic heterojunctions.Furthermore,the output voltage can be delicately adjusted and enhanced with the interface energy level engineering of inserting dielectric layers.Under the ultrahigh interface electric field,hot electrons will still transfer across the interface through the tunneling and hopping effect.In particular,the intrinsic anisotropy of black phosphorus arising from the lattice structure produces extraordinary electronic,transport,and mechanical properties exploited in our dynamic heterojunction generator.Herein,the voltage of 6.1 V,current density of 124.0 A/m^(2),power density of 201.0 W/m^(2),and energy-conversion efficiency of 31.4%have been achieved based on the dynamic black phosphorus/AlN/Si heterojunction,which can be used to directly and synchronously light up light-emitting diodes.This direct-current generator has the potential to convert ubiquitous mechanical energy into electric energy and is a promising candidate for novel portable and miniaturized power sources in the in situ energy acquisition field. | Yanghua Lu Sirui Feng Runjiang Shen Yujun Xu Zhenzhen Hao Yanfei Yan Haonan Zheng Xutao Yu Qiuyue Gao Panpan Zhang Shisheng Lin | 2019 | Research2019，，1： | 2 |
| 9 | Effects of inherent alkali and alkaline earth metals on nitrogen transformation during steam gasification of Shengli lignite显示文摘This work evaluated the effects of inherent alkali and alkaline earth metals on nitrogen transformation during steam gasification of Shengli lignite at the temperature of 873-1173 K in a fluidized-bed/fixed-bed quartz reactor. The results indicated that the alkali metal Na and alkaline earth metals Ca, Mg in coal have different effects on inherent nitrogen transformation to NH3, HCN and char-N during the lignite steam gasification. Specifically during the steam gasification of Shengli lignite, Na and Ca, Mg not only catalyze the inherent nitrogen conversions to NH3, but also promote the secondary reactions of the nascent char-N as well as the generation of NH3 from the generated HCN, meanwhile they also inhibited the inherent nitrogen conversion to HCN and char-N. The presence of Na, Ca and Mg hindered the formation of oxidized nitrogen (N-X) functional groups, but enhanced pyridinic nitrogen (N-6) and quaternary nitrogen's (N-Q) formation in char. | Panpan Zheng Yonggang Wang Chen Liu Weijie Guo Lei Bai Xiao Hu Xiongchao Lin | 2019 | International Journal of Coal Science & Technology2019，6，2： | 2 |
| 10 | Interfacial Built-In Electric Field-Driven Direct Current Generator Based on Dynamic Silicon Homojunction显示文摘Searching for light and miniaturized functional device structures for sustainable energy gathering from the environment is the focus of energy society with the development of the internet of things.The proposal of a dynamic heterojunction-based direct current generator builds up new platforms for developing in situ energy.However,the requirement of different semiconductors in dynamic heterojunction is too complex to wide applications,generating energy loss for crystal structure mismatch.Herein,dynamic homojunction generators are explored,with the same semiconductor and majority carrier type.Systematic experiments reveal that the majority of carrier directional separation originates from the breaking symmetry between carrier distribution,leading to the rebounding effect of carriers by the interfacial electric field.Strikingly,NN Si homojunction with different Fermi levels can also output the electricity with higher current density than PP/PN homojunction,attributing to higher carrier mobility.The current density is as high as 214.0 A/m^(2),and internal impedance is as low as 3.6 kΩ,matching well with the impedance of electron components.Furthermore,the N-i-N structure is explored,whose output voltage can be further improved to 1.3V in the case of the N-Si/Al2O3/N-Si structure,attributing to the enhanced interfacial barrier.This approach provides a simple and feasible way of converting low-frequency disordered mechanical motion into electricity. | Yanghua Lu Qiuyue Gao Xutao Yu Haonan Zheng Runjiang Shen Zhenzhen Hao Yanfei Yan Panpan Zhang Yu Wen Guiting Yang Shisheng Lin | 2020 | Research2020，，1： | 2 |
| 11 | Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment显示文摘 | Panpan Gao Daqing Mao Yi Luo Limei Wang Bingjie Xu Lin Xu | 2012 | Water Research2012，，7： | 1 |
| 12 | Facile hydrothermal-thermal conversion synthesis of CaSiO_3 nanowires as promising structure and function integrated photoluminescent host candidate显示文摘A facile green low-cost controllable hydrothermal-thermal conversion(HTC) process for the uniform high aspect ratio CaSiO_3 nanowires has been developed using abundant CaCl_2·2H_2O and Na_2 SiO_3·9H_2O as raw materials without any organic additive. The nanowires exhibited a transparent characteristic from the ultraviolet to visible region, and the CaSiO_3:1.2%Tb^(3+)nanophosphors with well preserved 1D morphology demonstrated strong green luminescence with CIE coordinates of(x=0.3144, y=0.5912).The present work definitely reveals the nanowires as a promising structure and function integrated host candidate for green-emitting luminescent materials in light display systems and optoelectronic devices. | Lin Xu Panpan Sun Xiuping Chen Peiyan Zhai Wancheng Zhu | 2019 | Chinese Chemical Letters2019，30，1： | 1 |
| 13 | A computational model of topological and geometric recovery for visual curve completion显示文摘Visual curve completion is a fundamental problem in understanding the principles of the human visual system. This problem is usually divided into two problems: a grouping problem and a shape problem.On one hand, though perception of the visually completed curve is clearly a global task(for example,a human perceives the Kanizsa triangle only when seeing all three black objects), conventional methods for solving the grouping problem are generally based on local Gestalt laws. On the other hand, the shape of the visually completed curve is usually recovered by minimizing shape energy in existing methods.However, not only do these methods lack mechanisms to adjust the shape of the recovered visual curve using perceptual, psychophysical, and neurophysiological knowledge, but it is also difficult to calculate an explicit representation of the visually completed curve. In this paper, we present a systematic computational model for generating a visually completed curve. Firstly, based on recent studies of perception, psychophysics, and neurophysiology, we formulate a grouping procedure based on the human visual system by seeking a minimum Hamiltonian cycle in a graph, solving the grouping problem in a global manner. Secondly, we employ a B′ezier curve-based model to represent the visually completed curve. Not only is an explicit representation deduced, but we also present a means to integrate knowledge from related areas, such as perception, psychophysics, and neurophysiology, and so on. The proposed computational model has been validated using many modal and amodal completion examples, and desirable results were obtained. | Hongwei Lin Zihao Wang Panpan Feng Xingjiang Lu Jinhui Yu | 2016 | Computational Visual Media2016，2，4： | 1 |
| 14 | Layered CuNi-Cu_(2)O/NiAlO_(x) nanocatalyst for rapid conversion of p-nitrophenol to p-aminophenol显示文摘In order to well arrange active sites and avoid byproducts, the reasonable structured carrier nanocatalyst plays a crucial role in high catalytic performance, but still remains a challenge. Herein, the layered CuNi-Cu_(2)O/NiAlO_(x) nanosheets have been constructed through hydrothermal synthesis followed by calcination and H_(2) reduction treatment process. The in-situ formed CuNi nanoalloys (NAs) and nano-Cu_(2)O were evenly distributed on the bilateral surface of layered NiAlOx nanosheets. Based on the planar structure of nanosheet, the synergy between catalytic active CuNi NAs and photocatalytic active nano-Cu_(2)O endows CuNi-Cu_(2)O/NiAlO_(x) nanosheets with rapid conversion efficiency for catalyzing p-nitrophenol (p-NP, 14 mg·L^(−1)) to p-aminophenol (p-AP) in 32 s with the reaction rate constant k up to 0.1779 s−1, and no obvious performance decay can be observed even over 27 cycles. Moreover, high concentration of p-NP at 10 and 20 g·L^(−1) could be reduced to p-AP within 14 and 20 min, respectively. Such designed nanoalloy/bimetal-oxide heterostructure can provide a solution for rapid conversion of aminoaromatics from nitroaromatics wastewater even at a large concentration range. | Lin Fu Wei Zhou Ming Wen Qingsheng Wu Weiying Li Dandan Wu Quanjing Zhu Jiaqi Ran Panpan Ren | 2021 | Nano Research2021，14，12： | 1 |
| 15 | Chronic Exposure to Environmentally Relevant Concentrations of Tetracycline Perturbs Gut Homeostasis in Zebrafish显示文摘The prevalence of residual antibiotics is a global threat to human health.Less is known about the potential health effects of residual antibiotics in freshwater systems.Here,zebrafish were used to explore chronic effects of environmentally relevant concentrations(ERCs)of tetracycline(TC).Although chronic exposure to TC did not significantly alter the body weight of adult zebrafish,the exposed zebrafish parents exhibited substantial changes in gut microbiota composition and a reduced gut-weight ratio.Notably,male fish exposed to TC showed a significant decline of critical intestinal function-related metabolites(i.e.,triglycerides,glucose,and free fat acid),while this was not observed in females,resulting in sex-dependency.The gut microbial composition of chronically exposed zebrafish parents changed substantially,but the disruption was not transferred to their respective offspring without exposure.However,the perturbation of insulinrelated signaling pathways caused by TC exposure was not attenuated in the zebrafish offspring after removal of TC exposure.Taken together,our findings suggest that chronic exposure to TC disturbs gut microbial communities and metabolism and exerts chronic effects on the insulin/IGF-1 mediated signaling cascades,implying that exposure to antibiotics not only leads to the selection of resistant microbes but also poses long-term deleterious health risks for the next generation. | Panpan Jia Shun Deng Xiaoxing Lin Liyan Song Yangqing Wang De-Sheng Pei | 2023 | Environment & Health2023，1，4： | 0 |
| 16 | Allogeneic gene-edited HIV-specific CAR-T cells secreting PD-1 blocking scFv enhance specific cytotoxic activity against HIV Envt cells in vivo显示文摘HIV-specific chimeric antigen receptor(CAR)T-cells have been developed to target HIV-1 infected CD4t T-cells that express HIV Env proteins.However,T cell exhaustion and the patient-specific autologous paradigm of CAR-T cell hurdled clinical applications.Here,we created HIV-specific CAR-T cells using human peripheral blood mononuclear cells and a 3BNC117-E27(3BE)CAR construct that enabled the expression of programmed cell death protein(PD-1)-blocking scFv E27 and the single-chain variable fragment of the HIV-1-specific broadly neutralizing antibody 3BNC117 to target native HIV Env.Compared with T cells expressing 3BNC117-CAR alone,3BE CAR-T cells showed greater cytotoxic activity against HIV Envt cells with stronger proliferation capability,higher killing efficiency,and enhanced cytokine secretion in the presence of HIV Env-expressing cells.Furthermore,we manufactured TCR-deficient 3BE CAR-T cells through gene editing and demonstrated that these CAR-T cells could effectively kill HIV Env^(+) cells in vivo without the occurrence of severe graft-versus-host disease(GvHD)in NSG mice.These data suggest that we have provided a feasible approach to the generation of“off-theshelf”anti-HIV CAR-T cells in combination with PD-1 checkpoint blockade immunotherapy,which can be a powerful therapeutic candidate for the functional cure of HIV. | Hanyu Pan Xinyi Yang Jing Wang Huitong Liang Zhengtao Jiang Lin Zhao Yanan Wang Zhiming Liang Xiaoting Shen Qinru Lin Yue Liang Jinglong Yang Panpan Lu Yuqi Zhu Min Li Pengfei Wang Jianqing Xu Hongzhou Lu Huanzhang Zhu | 2023 | Virologica Sinica2023，38，2： | 0 |
| 17 | Circulating biomarkers for nonfunctional gastroenteropancreatic neuroendocrine neoplasm:Where do we stand?显示文摘Gastroenteropancreatic neuroendocrine neoplasms(GEP-NENs) encompass a heterogeneous group of tumors associated with variable presentations, growth rates, and prognoses. The majority of GEP-NENs are nonfunctional, and their diagnosis remains challenging given the often subtle and variable clinical manifestations of these tumors. As a consequence, GEP-NENs are often recognized at an advanced stage; indeed, most patients with nonfunctional GEP-NENs exhibit metastatic disease at diagnosis. Lack of treatment options as well as limitations in currently available imaging modalities and biomarkers make it challenging to manage NENs. Thus, novel biomarkers are needed to provide high sensitivity and specificity for minimum disease detection and to predict treatment efficacy and prognosis. Although tissue-based biomarker data can provide such information, circulating biomarkers such as NETests, circulating tumor cells, and micro RNAs, are superior owing to their easy accessibility and the ability for repeated real-time sampling. | Panpan Zhang Lin Shen | 2017 | Oncology and Translational Medicine2017，3，1： | 0 |
| 18 | An efficient method to engage oxide ceramics in low-temperature interfacial reactions: Microstructure evolution and kinetics behaviors based on supercooling in a transient liquid phase bonding joint显示文摘Ductile transient liquid phase(TLP)bonding joints reinforced by multiple precipitates were produced using novel pre-sintered coatings and Au-Si fillers;therefore,the highest strength of NiTi/sapphire joints brazed at 460℃ for 30 min reached 72 MPa.The pre-sintering process improved the surface-active of sapphire by forming metastable Ti_(3)O and non-stoichiometric Al_(2)O_(3).The typical brazing seam consisted of O-rich compounds,TiSi_(2),and Ti-Ni-Si,wherein the O-rich phase featured different crystallinity depending on the oxygen content.The sapphire/seam interface was either a nanoscale diffusion region or a Si-rich amorphous layer.The breakdown of the Stokes-Einstein relation(SER)occurred,and the deviation from SER increased with a higher cooling rate.The influence of coating thickness was reflected in(i)the supercooling related to the viscosity and fractional exponent of liquids and(ii)the microstructural change of the joint related to the driving force for crystal growth.This work presented a new strategy for joining ceramics to metals at lower temperatures but using the joint at higher temperatures;furthermore,gave an insight into the microstructure evolution and kinetics behaviors based on supercooling in a transient liquid phase bonding joint. | Jia Yang Wanqi Zhao Panpan Lin Qiuguang Zhang Xinfei Zhang Tiesong Lin Peng He Yanli Zhuang | 2023 | Journal of Materials Science & Technology2023，，20： | 0 |
| 19 | Harnessing decellularised extracellular matrix microgels into modular bioinks for extrusion-based bioprinting with good printability and high post-printing cell viability显示文摘The printability of bioink and post-printing cell viability is crucial for extrusion-based bioprinting.A proper bioink not only provides mechanical support for structural fidelity,but also serves as suitable three-dimensional(3D)microenvironment for cell encapsulation and protection.In this study,a hydrogel-based composite bioink was developed consisting of gelatin methacryloyl(GelMA)as the continuous phase and decellularised extracellular matrix microgels(DMs)as the discrete phase.A flow-focusing microfluidic system was employed for the fabrication of cell-laden DMs in a high-throughput manner.After gentle mixing of the DMs and GelMA,both rheological characterisations and 3D printing tests showed that the resulting DM-GelMA hydrogel preserved the shear-thinning nature,mechanical properties,and good printability from GelMA.The integration of DMs not only provided an extracellular matrix-like microenvironment for cell encapsulation,but also considerable shear-resistance for high post-printing cell viability.The DM sizes and inner diameters of the 3D printer needles were correlated and optimised for nozzle-based extrusion.Furthermore,a proof-of-concept bioink composedg of RSC96 Schwann cells encapsulated DMs and human umbilical vein endothelial cell-laden GelMA was successfully bioprinted into 3D constructs,resulting in a modular co-culture system with distinct cells/materials distribution.Overall,the modular DM-GelMA bioink provides a springboard for future precision biofabrication and will serve in numerous biomedical applications such as tissue engineering and drug screening. | Hanyu Chu Kexin Zhang Zilong Rao Panpan Song Zudong Lin Jing Zhou Liqun Yang Daping Quan Ying Bai | 2023 | Biomaterials Translational2023，4，2： | 0 |
| 20 | Correction: Autophagy receptor CCDC50 modulates STING-mediated interferon response in viral infection and autoimmune disease显示文摘Correction to:Cellular&Molecular Immunology http://gffzzd3cc09b8251d45dfsfcvxcxuxnwfc6v9c.ffgz.tsg.suse.edu.cn/10.1038/s41423-021-00758-w In the version of this article initially published,one unintended error was made during manuscript editing.The title of the article was not in accordance with that of the original manuscript,and the correct statement is“Autophagy receptor CCDC50 modulates STING-mediated interferon response in viral infection and autoimmune disease”.The results and conclusions are not affected. | Panpan Hou Yuxin Lin Zibo Li Ruiqing Lu Yicheng Wang Tian Tian Penghui Jia Xi Zhang Liu Cao Zhongwei Zhou Chunmei Li Jieruo Gu Deyin Guo | 2021 | Cellular & Molecular Immunology2021，18，12： | 0 |
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