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| 1 | Metabolic shift in liver: Correlation between perfusion temperature and hypoxia inducible factor-1α显示文摘AIM: To study at what temperature the oxygen carried by the perfusate meets liver requirements in a model of organ perfusion. METHODS: in this study, we correlated hypoxia induciblefactor(Hi F)-1α expression to the perfusion temperature and the hepatic oxygen uptake in a model of isolated perfused rat liver. Livers from Wistar rats were perfused for 6 h with an oxygenated medium at 10, 20, 30 and 37 ℃. Oxygen uptake was measured by an oxygen probe; lactate dehydrogenase activity, lactate release and glycogen were measured spectrophotometrically; bile flow was gravitationally determined; p H of the perfusate was also evaluated; Hi F-1α m RNA and protein expression were analyzed by real time-polymerase chain reaction and ELi SA, respectively. RESULTS: Livers perfused at 10 and 20 ℃ showed no difference in lactate dehydrogenase release after 6 h of perfusion(0.96 ± 0.23 vs 0.93 ± 0.09 m U/min per g) and had lower hepatic damage as compared to 30 and 37 ℃(5.63 ± 0.76 vs 527.69 ± 45.27 m U/min per g, respectively, P s < 0.01). After 6 h, tissue ATP was significantly higher in livers perfused at 10 and 20 ℃than in livers perfused at 30 and 37 ℃(0.89 ± 0.06 and 1.16 ± 0.05 vs 0.57 ± 0.09 and 0.33 ± 0.08 nmol/mg, respectively, P s < 0.01). No sign of hypoxia was observed at 10 and 20 ℃, as highlighted by low lactate release respect to livers perfused at 30 and 37 ℃(121.4 ± 12.6 and 146.3 ± 7.3 vs 281.8 ± 45.3 and 1094.5 ± 71.7 nmol/m L, respectively, P s < 0.02), and low relative Hi F-1α m RNA(0.40 ± 0.08 and 0.20 ± 0.03 vs 0.60 ± 0.20 and 1.47 ± 0.30, respectively, P s < 0.05) and protein(3.72 ± 0.16 and 3.65 ± 0.06 vs 4.43 ± 0.41 and 6.44 ± 0.82, respectively, P s < 0.05) expression.CONCLUSION: Livers perfused at 10 and 20 ℃ show no sign of liver injury or anaerobiosis, in contrast to livers perfused at 30 and 37 ℃. | Andrea Ferrigno Laura Giuseppina Di Pasqua Alberto Bianchi Plinio Richelmi Mariapia VairettiAndrea Ferrigno | 2015 | World Journal of Gastroenterology2015，21，4： | 5 |
| 2 | Localization and role of metabotropic glutamate receptors subtype 5 in the gastrointestinal tract显示文摘Metabotropic glutamate receptor subtype 5(mGluR5) is a Group I mGlu subfamily of receptors coupled to the inositol trisphosphate/diacylglycerol pathway. Like other m Glu R subtypes, mGluR5 s contain a phylogenetically conserved, extracellular orthosteric binding site and a more variable allosteric binding site, located on the heptahelical transmembrane domain. The mGluR5 receptor has proved to be a key pharmacological target in conditions affecting the central nervous system(CNS) but its presence outside the CNS underscores its potential role in pathologies affecting peripheral organs such as the gastrointestinal(GI) tract and accessory digestive organs such as the tongue, liver and pancreas. Following identification of mGluR5s in the mouth, various studies have subsequently demonstrated its involvement in mechanical allodynia, inflammation, pain and oral cancer. mGluR5 expression has also been identified in gastroesophageal vagal pathways. Indeed, experimental and human studies have demonstrated that mGluR5 blockade reduces transient lower sphincter relaxation and reflux episodes. In the intestine, mGluR5s have been shown to be involved in the control of intestinal inflammation, visceral pain and the epithelial barrier function. In the liver, mGluR5s have a permissive role in the onset of ischemic injury in rat and mice hepatocytes. Conversely, livers from mice treated with selective negative allosteric modulators and mGluR5 knockout mice are protected against ischemic injury. Similar results have been observed in experimental models of free-radical injury and in vivo mouse models of acetaminophen intoxication. Finally, mGluR5s in the pancreas are associated with insulin secretion control. The picture is, however, far from complete as the review attempts to establish in particular as regards identifying specific targets and innovative therapeutic approaches for the treatment of GI disorders. | Andrea Ferrigno Clarissa Berardo Laura G Di Pasqua Veronica Siciliano Plinio Richelmi Mariapia Vairetti | 2017 | World Journal of Gastroenterology2017，23，25： | 3 |
| 3 | Liver plays a central role in asymmetric dimethylargininemediated organ injury显示文摘Asymmetric-dimethylarginine(ADMA) competes with L-arginine for each of the three isoforms of nitric oxide synthase:endothelial;neuronal;inducible.ADMA is synthesized by protein methyltransferases followed by proteolytic degradation.ADMA is metabolized to citrulline and dimethylamine,by dimethylarginine dimethylaminohydrolase(DDAH) and enters cells through cationic amino-acid transporters extensively expressed in the liver.The liver plays a crucial role in ADMA metabolism by DDAH-1 and,as has been recently demonstrated,it is also responsible for ADMA biliary excretion.A correlation has been demonstrated between plasma ADMA levels and the degree of hepatic dysfunction in patients suffering from liver diseases with varying aetiologies:plasma ADMA levels are increased in patients with liver cirrhosis,alcoholic hepatitis and acute liver failure.The mechanism by which liver dysfunction results in raised ADMA concentrations is probably due to impaired activity of DDAH due to severe inflammation,oxidative stress,and direct damage to DDAH.High plasma ADMA levels are also relevant as they are associated with the onset of multiorgan failure(MOF).Increased plasma concentration of ADMA was identified as an independent risk factor for MOF in critically-ill patients causing enhanced Intensive Care Unit mortality:a significant reduction in nitric oxide synthesis,leading to malperfusion in various organs,eventually culminating in multi organs dysfunction. | Andrea Ferrigno Laura G Di Pasqua Clarissa Berardo Plinio Richelmi Mariapia Vairetti | 2015 | World Journal of Gastroenterology2015，21，17： | 2 |
| 4 | Effects of the cul-ture medium pH and ion uptake in in vitro vegetative orga-nogenesis in thin cell layers of tobacco显示文摘 | Pasqua G Manes F Monacelli B | 2002 | Plant Science2002，162，6： | 1 |
| 5 | Cytotoxicity of mesoporous silica nanomaterials显示文摘 | Di Pasqua AJ Sharma KK Shi YL | | 0，，： | 1 |
| 6 | Three-vessel leftventricular microfistulization syndrome: a rare case of angina 显示文摘 | Iadanza A del Pasqua A Fineschi M | 2004 | Int J Cardiol2004，96，1： | 1 |
| 7 | Influence of NaOH andKOH on the synthesis of MCM-22 and MCM-49 zeolites显示文摘 | Vuono D Pasqua L Testa F | 2006 | Microporous and Mesoporous Materials2006，97，： | 1 |
| 8 | CPT accumulation in the fruit and during early phases of plant development inCamptotheca acuminata Decaisne (Nyssaceae) 显示文摘 | Valletta A Santamaria A R Pasqua G | 2007 | Natural Product Research2007，21，14： | 1 |
| 9 | Combining triple therapy and pulmonary rehabilitation in patients with advanced COPD: a pilot study显示文摘 | Pasqua F Biscione G Crigna G | 2010 | Respir Med2010，104，3： | 1 |
| 10 | Prognostic value of decremental responses to repetitive nerve stimulation in ALS patients显示文摘 | Wang FC De Pasqua V Gérard P | 2001 | Neurology2001，57，5： | 1 |
| 11 | Sensitization of non-small cell lung cancer cells to cisplatin by naturally occurring isothiocyanates显示文摘 | Di Pasqua AJ Hong C Wu MY | 2010 | Chem Res Toxicol2010，23，8： | 1 |
| 12 | Synthesis and Characterization of Amorphous Hydrated FePO4 and Its Electrode Performance in Lithium Batteries 显示文摘 | Prosini P P Zane D PasQua li M | 2002 | Electrochem Soc2002，149，： | 1 |
| 13 | An Empirical Analysis of the Time Allocation of Italian Couples: Are TheyResponsive? 显示文摘 | BloemenH PasquaS StancanelliE | 2010 | Review of Economics of the Household2010，，8： | 1 |
| 14 | Analysis of Class III Peroxidase Genes Expressed in Roots of Resistant and Susceptible Wheat Lines Infected by Heterodera avenae显示文摘 | Ester S Pasqua V | 2009 | Molecular Plant-MicrobeInteractions2009，22，9： | 1 |
| 15 | Pharmacoki-netic-pharmacodynamic correlations and biomarkers in the development of COX-2inhibitors显示文摘 | Huntjens DR Danhof M Della Pasqua OE | 2005 | Rheumatology(Oxford)2005，44，7： | 1 |
| 16 | Metabolites in cell suspension cultures, calli, and in vitro regenerated organs of Hypericum perforatum cv. Topas显示文摘 | Gabriella Pasqua Pinarosa Avato Barbara Monacelli Anna Rita Santamaria Maria Pia Argentieri | 2003 | Plant Science2003，，5： | 1 |
| 17 | GPER mediates cardiotropic effects in spontaneously hypertensive rat hearts 显示文摘 | De Francesco EM Angelone T Pasqua T | 2013 | PLoS One2013，8，69： | 1 |
| 18 | The effect of growth regulators and sucrose on anthocyanin production in Camptotheca acuminata cell cultures显示文摘 | Gabriella Pasqua Barbara Monacelli Nadia Mulinacci | 2005 | Plant Physiol Bioch2005，43，3： | 1 |
| 19 | Use of Functional Independence Measure in Rehabilitation of Inpatients with Respiratory Failure显示文摘 | Pasqua F Biscione G L Crigna G | 2009 | Respir Med2009，103，3： | 1 |
| 20 | Cell suspension cultures of Macl ura pomif era:Optimization of growth and metabolite production显示文摘 | Pasqua G Monacelli B Cuteri A | 1991 | J Plant Physiol1991，139，： | 1 |
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