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| 1 | Curcumin Reverses 5-Fluorouracil Resistance by Promoting Human Colon Cancer HCT-8/5-FU Cell Apoptosis and Down-regulating Heat Shock Protein 27 and P-Glycoprotein显示文摘Objective: To investigate the potential mechanisms that curcumin reverses 5-fluorouracil(5-FU) multidrug resistance(MDR). Methods: Cell growth and the inhibitory rate of curcumin(2–25 μg/mL) and/or5-FU(0.05–1000 μg/mL) on human colon cancer HCT-8 and HCT-8/5-FU(5-FU-resistant cel line) were determined using cel counting kit-8(CCK-8) assay. Apoptosis and cel cycle after 5-FU and/or curcumin treatment were detected by ?ow cytometry(FCM) and transmission electron microscopy(TEM). The expression of the multidrug resistance related factors p-glycoprotein(P-gp) and heat shock protein 27(HSP-27) genes and proteins were analyzed by reverse transcription polymerase chain reaction(RT-PCR) and Western blotting(WB), respectively. Results: The inhibitory rate of curcumin or 5-FU on HCT-8 and HCT-8/5-FU cells proliferation at exponential phase were in a dosedependent manner, HCT-8 cell line was more sensitive to curcumin or 5-FU when compared the inhibitory rate of HCT-8/5-FU. The 50% inhibitory concentration(IC50) of combination 5-FU and curcumin(4.0 μg/mL)in HCT-8/5-FU was calculated as 179.26 μg/mL, with reversal fold of 1.85. Another IC50 of combination 5-FU and curcumin(5.5 μg/mL) in HCT-8/5-FU was calculated as 89.25 μg/mL, with reversal fold of 3.71. Synergistic effect of 5-FU and curcumin on HCT-8 and HCT-8/5-FU cells were found. The cell cycle analysis performed by FCM showed that HCT-8 and HCT-8/5-FU cel s mostly accumulated at G0/G1 phase, which suggested a synergistic effect of curcumin and 5-FU to induce apoptosis. FCM analysis found that the percentage of apoptosis of cel s treated with curcumin, 5-FU and their combination were signi?cantly increased compared to the control group(P<0.05), and the percentage of apoptosis of the combination groups were slightly higher than other groups(P<0.05). The m RNA levels of P-gp(0.28±0.02) and HSP-27(0.28±0.09) in HCT-8/5-FU cel s treated with combination drugs were lower than cel s treated with 5-FU alone(P-gp, 0.48±0.07, P=0.009;HSP-27, 0.57±0.10, P=0.007). The protein levels of P-gp(0.25±0.06) and HSP-27(0.09±0.02) in HCT-8/5-FU cells treated with combination drugs were decreased when compared to 5-FU alone(P-gp, 0.46±0.02, P=0.005;HSP-27, 0.43±0.01, P=0.000). Conclusions: Curcumin can inhibit the proliferation of human colon cancer cells. Curcumin has the ability of reversal effects on the multidrug resistance of human colon cancer cells lines HCT-8/5-FU. Down-regulation of P-gp and HSP-27 may be the mechanism of curcumin reversing the drug resistance of HCT-8/5-FU to 5-FU. | HE Wen-ting ZHU Yan-hua ZHANG Tong ABULIMITI Patima ZENG Fan-ye ZHANG Li-ping LUO Ling-juan XIE Xin-mei ZHANG Hong-liang | 2019 | Chinese Journal of Integrative Medicine2019，25，6： | 6 |
| 2 | Cyclodextrin Polymer Films Optical Waveguide Sensor for Volatile Organic Gas Detection显示文摘A sensitive optical waveguide(OWG) sensor which can be used to detect volatile organic compounds(VOCs) was presented.The sensing device(element) was fabricated by means of the immobilization of polyvinyl pyrrolidone(PVP)-cyclodextrin(CD) composite film over a single-mode potassium ion exchanged glass OWG via spin-coating method.The sensor shows higher response to styrene gas than to other VOCs and displays a linear response to styrene gas in a range of 1―1000 μL/L. | YASIN Patime YIMIT Abliz RAHMAN Ebeyla NIZAMIDIN Patima | 2012 | Chemical Research in Chinese Universities2012，28，4： | 6 |
| 3 | Two new cardenolide glycosides from Cotreblus asper 显示文摘 | YOSHIO H PATIMA J ZEPER A | 2003 | Heterocycles2003，59，2： | 1 |
| 4 | Zinc Phthalocyanine Thin Film-Based Optical Waveguide H_(2)S Gas Sensor显示文摘The detection of hydrogen sulfide(H_(2)S)is essential because of its toxicity and abundance in the environment.Hence,there is an urgent requisite to develop a highly sensitive and economical H_(2)S detection system.Herein,a zinc phthalocyanine(ZnPc)thin film-based K+-exchanged optical waveguide(OWG)gas sensor was developed for H_(2)S detection by using spin coating.The sensor showed excellent H_(2)S sensing performance at room temperature with a wide linear range(0.1 ppm 500 ppm),reproducibility,stability,and a low detection limit of 0.1ppm.The developed sensor showed a significant prospect in the development of cost-effective and highly sensitive H_(2)S gas sensors. | Kediliya WUMAIER Gulgina MAMTMIN Qingrong MA Asiya MAIMAITI Patima NIZAMIDIN Abliz YIMIT | 2022 | Photonic Sensors2022，12，1： | 1 |
| 5 | Upregulation of beta2-microglobulinexpression in progressive human oral squamous cell carcinoma 显示文摘 | Jiang Q Patima S Ye DX | 2012 | On-col Hep2012，27，4： | 1 |
| 6 | Detection of Amines in Lamb Spoilage by Optical Waveguide Sensor Based on Bromophenol Blue-Silicon Composite Film显示文摘We herein report the development of a bromophenol blue(BPB)-silicone composite film/K^+-exchange glass optical waveguide(OWG)sensor for the detection of amines produced during the spoilage of lamb.the optical and structural properties of the sensitive thin film were studied by ultra violet-visible(UV-Vis)spectroscopy,and the light source of the OWG detecting system was selected.Gas sensing measurements showed that the sensor exhibited a good selectivity,higli sensitivity,and short response-recovery time towards volatile amine gases in the 0.00117一11.72 mg/g range.The as-prepared optical waveguide device was subsequently applied in the determination of gases(namely trimethylamine,dimethylamine,and ammonia)emitted from the lamb samples(5g)stored at room temperature(25℃)and in a refrigerator(5℃)for 0—4 d,and the total volatile basic nitrogen(TVB-N)contents were detected by UV-Vis spectroscopy,and the results were compared witli those obtained using our detector.It was found that the sensing element was capable of detecting mixed gases produced by the decomposition of lamb samples in a refrigerator for 0.5 h,where the TVB-N content was lower than 35μg/g. | KOXMAK Sayyara YIMAMUMAIMAITI Tajiguli ABDUKEREMU Hannikezi NIZAMIDIN Patima YIMIT Abliz | 2019 | Chemical Research in Chinese Universities2019，35，2： | 1 |
| 7 | Stress and hippocampal neurogenesis显示文摘 | Elizabeth Gould Patima Tanapat | 1999 | Biological Psychiatry1999，，11： | 1 |
| 8 | Exertional Heatstroke: Early Recognition and Outcome With Aggressive Combined Cooling - a 12-Year Experience显示文摘 | Sithinamsuwan Pasiri Piyavechviratana Kunchit Kitthaweesin Thitisak Chusri Watcharapong Orrawanhanothai Patima Wongsa Adisorn Wattanathum Anan Chinvarun Yotin Nidhinandana Samart Satirapoj Bancha Supasyndh Ouppatham Sriswasdi Chantrapa Prayoon | 2009 | Military Medicine2009，，5： | 1 |
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