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| 1 | Comparative WGBS identifies genes that influence non-ripe phenotype in tomato epimutant Colourless non-ripening显示文摘Whole-genome bisulfite sequencing(WGBS)allows single-base resolution and genome-wide profiling of DNA methylation in plants and animals.This technology provides a powerful tool to identify genes that are potentially controlled by dynamic changes of DNA methylation and demethylation.However,naturally occurring epimutants are rare and genes under epigenetic regulation as well as their biological relevances are often difficult to define.In tomato,fruit development and ripening are a complex process that involves epigenetic control.We have taken the advantage of the tomato epimutant Colourless non-ripening(Cnr)and performed comparative mining of the WGBS datasets for the Cnr and Sl CMT3-silenced Cnr fruits.We compared DNA methylation profiles for the promoter sequences of approximately 5,000 bp immediately upstream of the coding region of a list of20 genes.Differentially methylated regions were found for some of these genes.Virus-induced gene silencing(VIGS)of differentially methylated gene Sl DET1 or Sl PDS resulted in unusual brown pigmentation in Cnr fruits.These results suggest that comparative WGBS coupled with VIGS can be used to identify genes that may contribute to the colourless unripe phenotype of fruit in the Cnr epimutant. | Weiwei Chen Zhiming Yu Junhua Kong Hui Wang Yichen Li Mei Zhao Xiaohong Wang Qianqian Zheng Nongnong Shi Pengcheng Zhang Silin Zhong Paul Hunter Mahmut Tor Yiguo Hong | 2018 | Science China(Life Sciences)2018，61，2： | 3 |
| 2 | Effect of melt processing conditions on the extent of exfoliation in organoclay-based nanocomposites显示文摘 | H.R. Dennis D.L. Hunter D. Chang S. Kim J.L. White J.W. Cho D.R. Paul | 2001 | Polymer2001，，23： | 2 |
| 3 | Nylon-6 Nano- composites from Alkylammonium-modified Clay: The Role of Alkyl Tails on Exfoliation 显示文摘 | FORNES T D HUNTER D L PAUL D R | 2004 | Macromolecules2004，37，5： | 1 |
| 4 | Effect of sodium montmorillonite source on nyton6/clay nanocomposites显示文摘 | Fornes T D Hunter D L Paul D R | 2004 | Polymer2004，45，7： | 1 |
| 5 | Nylon-6 Nanocomposites from Alkylammonium-Modified Clay:The Role of Alkyl Tails on Exfoliation显示文摘 | Fornes T D Hunter D L Paul D R | 2004 | Macromolecules2004，37，： | 1 |
| 6 | Theme Article-Processing and Properties of Polymers Modified by Clays显示文摘 | Douglas L Hunter Karl W Kamena Paul D R | 2007 | MRS Bull2007，82，： | 1 |
| 7 | Polycarbonate nanocomposites,Part 2:Degradation and color formation显示文摘 | YOON P J HUNTER D L PAUL D R | 2003 | Polymer2003，44，18： | 1 |
| 8 | Polycarbonate nanocomposites,Part 1:Effect of organoclay structure on morphology and properties显示文摘 | YOON P J HUNTER D L PAUL D R | 2003 | Polymer2003，44，18： | 1 |
| 9 | Polycarbonate nanocomposites Part1: Effect of organoclay structure on morphology and properties 显示文摘 | Yoon P J Hunter D L Paul D R | 2003 | Polymer2003，44，18： | 1 |
| 10 | Management of Intracanal Separated Instruments显示文摘 | Ahmad Abdulhameed Madarati Mark J. Hunter Paul M. Dummer | 2013 | Journal of Endodontics2013，，： | 1 |
| 11 | Effect of organoclay structure on nylon 6 nanocomposite morphology and properties显示文摘 | Fornes T D Yoon P J Hunter D L Keskkula H Paul D R | 2002 | Polymer2002，43，： | 1 |
| 12 | Direct Measurement of Atmosphere Formaldehyde Using Gas Chromatography-Pulsed Discharge Lonisation Detection显示文摘 | Keith D Battle Paul W Seakins | 1996 | Analytical Communication1996，36，2： | 1 |
| 13 | Effect of sodium montmorillonite source on nylon 6/clay nanocomposites显示文摘 | Fornes T D Hunter D L Paul D R | 2004 | Polymer2004，45，7： | 1 |
| 14 | Establishment of a universal size standard strain for use with the PulseNet Standardized Pulsed-Field gel electrophoresis Protocols: Converting the National Databases to the New Size Standard显示文摘 | Hunter Susan B Vauterln Paul Lambert-Fair Mary Ann | 2005 | J Clin Microbio2005，43，3： | 1 |
| 15 | Epidemiology and clinical features of Cryptosporidium infection in immunocompromised patients显示文摘 | Paul R Hunter Gordon Nichols | 2002 | Clinical Microbiology Reviews2002，15，1： | 1 |
| 16 | Revealing CO_(2)-Fixing SAR11 Bacteria in the Ocean by Raman-Based Single-Cell Metabolic Profiling and Genomics显示文摘The majority of marine microbes remain uncultured,which hinders the identification and mining of CO_(2)-fixing genes,pathways,and chassis from the oceans.Here,we investigated CO_(2)-fixing microbes in seawater from the euphotic zone of the Yellow Sea of China by detecting and tracking their ^(13)C-bicarbonate(^(13)C-HCO_(3)^(-))intake via single-cell Raman spectra(SCRS)analysis.The target cells were then isolated by Raman-activated Gravity-driven Encapsulation(RAGE),and their genomes were amplified and sequenced at one-cell resolution.The single-cell metabolism,phenotype and genome are consistent.We identified a notyet-cultured Pelagibacter spp.,which actively assimilates ^(13)C-HCO_(3)^(-),and also possesses most of the genes encoding enzymes of the Calvin-Benson cycle for CO_(2) fixation,a complete gene set for a rhodopsin-based light-harvesting system,and the full genes necessary for carotenoid synthesis.The four proteorhodopsin(PR)genes identified in the Pelagibacter spp.were confirmed by heterologous expression in E.coli.These results suggest that hitherto uncultured Pelagibacter spp.uses light-powered metabolism to contribute to global carbon cycling. | Xiaoyan Jing Yanhai Gong Teng Xu Paul ADavison Craig MacGregor-Chatwin CNeil Hunter La Xu Yu Meng Yuetong Ji Bo Ma Jian Xu Wei E.Huang | 2022 | BioDesign Research2022，，1： | 1 |
| 17 | Establishment of a universal size standard strain for use with the PulseNet Standardized Pulsed - Field Gel Electrophoresis Protocols: converting the national databases to the new size standard 显示文摘 | Hunter Susan B Vauterin Paul Lambert - Fair Mary Ann | 2005 | J Clin Microbio2005，43，3： | 1 |
| 18 | Nanocomposites from poly(ethylene-co-methacrylic acid) ionomers: effect of surfactant structure on morphology and properties 显示文摘 | SHAH R K HUNTER D L PAUL D R | 2005 | Polymer2005，46，8： | 1 |
| 19 | Defect detection using ultrasonic arrays: The multi-mode total focusing method显示文摘 | Jie Zhang Bruce W. Drinkwater Paul D. Wilcox Alan J. Hunter | 2009 | NDT and E International2009，，2： | 1 |
| 20 | Establishment of a Universal Size Strain for Use with the PulseNet Standardized Pulsed-Field Gel Electrophoresis Protocols: Converting the National Databases to the New Size Slandard 显示文摘 | Hunter Susan B Vauterin Paul Lambert-Fair Mary Ann | 2005 | J Clin Microbiol2005，43，： | 1 |
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