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| 1 | Visual characterization of targeted effect of holo-transferrin-tagged dihydroartemisinin on human breast cancer cells显示文摘Targeted drugs could significantly reduce cytotoxic effect and increase therapeutic activity. Dihydroartemisinin (DHA) has been shown to be effective in killing cancer cells. However, it exhibits non-targeted property. Holo-transferrin (TF) is a suitable drug-carrier to target cancer cells, because cancer cells need iron uptake by the TF-mediated mechanism to maintain their uncontrolled growth. Furthermore, TF receptors (TF-R) are highly expressed on cancer cell surfaces. In this paper, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the different killing effect of 4-(12-Dihydroartemisininoxy) Benzoic Acid Hydrozide-transferrin (DBAH-TF) on human breast cancer cells (MCF-7) cells and human normal breast (HNB) cells, and atomic force microscopy (AFM) was used to visually observe the targeted effect of DBAH-TF on MCF-7 cells. MTT results show that DBAH-TF is 172 times more potent than DHA in killing MCF-7 cells, while the cytotoxic effect of DBAH-TF on HNB cells is merely 1/33 to DHA. Also, the killing effect of DBAH-TF on MCF-7 cells is 286 times that on HNB cells, showing targeted effect. Moreover, there are distinct differences in ultrastructures of cellular surfaces after DBAH-TF and DHA treatment. Through AFM imaging, many characteristic holes were observed on the cancer cell surface after being effected by DBAH-TF, which differ from the holes with irregular shapes affected by DHA. These results visually show that the DBAH-TF targeted drug has more potent killing effect on MCF-7 cells compared with DHA. | XIE WeiLing YANG PeiHui ZENG Xin WANG Hui CAI HuaiHong CAI JiYe | 2010 | Chinese Science Bulletin2010，55，22： | 4 |
| 2 | PROBING MOLECULAR INTERACTION BETWEEN TRANSFERRIN AND ANTI-TRANSFERRIN BY ATOMIC FORCE MICROSCOPE显示文摘The interaction between transferrin (Tf) and its antibody was investigated by atomic force microscope. Tf-antibody was immobilized on the Au-coated glass slide, and the specific combination between antibody and antigen was also character-ized by AFM. The results showed that holo-transferrin was jogged with anti-transferrin, and binded anti-tran- sferrin more tightly than apo-transferrin. The force- distance curves revealed that the affinity of anti-trans- ferrin and holo-transferrin was much stronger than that of apo-transferrin. | ZHENG Zhiwen YANG Peihui ZENG Gucheng CAI Jiye | 2006 | Chinese Science Bulletin2006，51，4： | 3 |
| 3 | Nanostructures and molecular force bases of a highly sensitive capacitive immunosensor显示文摘 | ZENG Gucheng YANG Peihui ZHENG Zhiwen | 2005 | Proteomics2005，5，17： | 1 |
| 4 | 显示文摘 | Liu Yingling Feng Dexiong Yang Peihui | 2000 | Rare Metals2000，19，4： | 1 |
| 5 | 显示文摘 | Liu Yingliang Feng Dexiong Yang Peihui | 2000 | Rare Metal2000，19，4： | 1 |
| 6 | A CD44-biosensor for evaluating metastatic potential of breast cancer cells based on quartz crystal microbalance显示文摘A sensitive CD44-biosensor based on quartz crystal microbalance(QCM) was proposed for evaluating metastatic potential of breast cancer cells by using hyaluronan(HA) functionalized substrate film,polydopamine and polyethyleneimine composite film,for the purpose of capturing CD44-positive cancer cells through specific binding of HA to CD44.Two differently CD44-expressed breast cancer cell lines(MDAMB-231 cells and MCF-7 cells) were put to use as targets for quantitative analysis as well as evaluation of metastatic potential of the cells.The limit of detection for MDA-MB-231(M231) cells and MCF-7 cells were 300 and 1,000 cells mL ~1 respectively.The expression level of CD44 on M231 cells exhibited two times higher than that of MCF-7 cells,indicating of a higher metastatic potential.Moreover,poly-Llysine modified QCM sensor was applied to monitor the stiffness of breast cancer cells that can reflect metastatic potential of cells.The results revealed that the MCF-7 cells were stiffer than M231 cells,implying that the M231 cells possessed higher metastatic potential.The proposed protocol is simple and rapid to evaluate the metastatic potential of cancer cells,in addition to offering a promising diagnostic tool for metastatic cancer. | Xiaojuan Yang Rongcheng Zhou Yan Hao Peihui Yang | 2017 | Science Bulletin2017，62，13： | 0 |
| 7 | Supramolecular assemblies of cucurbit[n]urils and 4-aminopyridine controlled by cucurbit[n]uril size(n=5,6,7 and 8)显示文摘The binding interactions between 4-aminopyridine(4-AP) and a series of cucurbit[n]urils(Q[5], Q[6],TMe Q[6], Q[7], Q[8]) have been studied using1H NMR spectroscopy, UV–vis absorption spectroscopy,isothermal titration calorimetry(ITC) and X-ray crystallography. The data indicates that the Q[5]@4-AP complex exhibits exo binding, which is not observed in the other four host-guest complexes. Furthermore,X-ray crystallography clearly reveals how the Q[n]s bind with 4-AP to form complexes, for example Q[5]forms an outer-surface complex, whilst Q[6], TMe Q[6] and Q[7] formed 1:1 host and guest type complexes, and Q[8] formed a stable 1:2 ternary complex due to its large cavity, which can accommodate two 4-AP molecules. | Yun Lu Zhichao Yu Xinan Yang Jingjing Dai Peihui Shan Xianhao Feng Zhu Tao Carl Redshaw Xin Xiao | 2023 | Chinese Chemical Letters2023，34，7： | 0 |
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