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您的检索式：作者名="Petr V.SERGIEV"
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| 1 | Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert显示文摘Three hundred and twenty endophytic actinobacterial strains were isolated from psammophytes collected from Taklamakan Desert and identified. Among them, three strains already had been identified as new species of two genera and sixteen isolates showed relatively low 16 S rRNA similarities < 98.6% to validly described species. Seventy-five of the isolates were selected as representative strains to screen antibacterial activity and mechanism. Forty-seven strains showed antagonistic activity against at least one of the indicator bacteria. Two Streptomyces strains produced bioactive compounds inducing DNA damage, and two Streptomyces strains produced bioactive compounds with inhibitory activity on protein biosynthesis. Notably, the strain Streptomyces sp. 8P21H-1 that demonstrated both strong antibacterial activity and inhibitory activity on protein biosynthesis was prioritized for exploring new antibiotics.Under the strategy of integrating genetics-based discovery program and MS/MS-based molecular networking, two new streptogramin-type antibiotics, i.e., acetyl-griseoviridin and desulphurizing griseoviridin, along with known griseoviridin, were isolated from the culture broth of strain 8P21H-1. Their chemical structures were determined by HR-MS, and 1D and 2D NMR. Desulphurizing griseoviridin and griseoviridin exhibited antibacterial activities by inhibiting translation. | Ting Wang Feina Li Qinpei Lu Gang Wu Zhongke Jiang Shaowei Liu Xugela Habden Elizaveta A.Razumova Ilya A.Osterman Petr V.Sergiev Olga A.Dontsova Xinxin Hu Xuefu You Chenghang Sun | 2021 | Journal of Pharmaceutical Analysis2021，11，2： | 2 |
| 2 | A pilot survey on antimicrobial activity and diversity of soil-derived actinobacteria from different depths in Gudzhirganskoe saline lake in Eastern Siberia显示文摘To explore pharmaceutical actinobacteria from salty environments in Siberia,8 soil samples were collected from different depths of Gudzhirganskoe saline lake,the Republic of Buryatia,Russia.Totally,635 actinobacterial strains affiliated with 21 genera in 12 families of 7 orders were obtained by culture-dependent approaches.The predominant genus was Streptomyces(74.5%),followed by Microbacterium(4.9%),Agromyces(3.5%),Nocardiopsis(3.0%),and Kitasatospora(2.5%).Seven strains showed relatively low 16 S rRNA similarities(<98.65%)with validly described species.Seventy-seven strains were selected as representatives to evaluate their antimicrobial activities and underlying mechanisms by paper-disk diffusion method and a double fluorescent protein reporter system(pDualrep 2),respectively.Streptomyces sp.S 6 b 3-1 was highlighted due to its strong inhibitory activities against Gram-positive bacteria and underlying antibacterial mechanism by inducing the SOS response in the reporter system.LG-1,(R)-6-[1-(benzoyloxy)ethyl]-phenazine-1-carboxylic acid,an analogue of saphenamycin was finally isolated from the culture broth of Streptomyces strain.This study preliminarily explored the actinobacterial diversity of soil samples collected in the highly alkaline and saline lake Gudzhirganskoe by culture-dependent approach and their capacity to produce antibiotics for the first time,which revealed that it deserves to make more efforts to discover new actinobacterial species and potential new antibiotics from the saline lake in Eastern Siberia. | Qin YANG Puyu GUO Elena Y.ABIDUEVA Elena V.LAVRENTYEVA Irina M.LISEVICH Ilya A.OSTERMAN Petr V.SERGIEV Shaowei LIU Chunmei XUE Chenghang SUN | 2023 | Journal of Oceanology and Limnology2023，41，4： | 0 |
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