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| 1 | Kaempferol protects ethanol-induced gastric ulcers in mice via pro-inflammatory cytokines and NO显示文摘胃的溃疡(GU ) 是在全世界影响许多人的普通病理。过多的白酒消费是 GU 的主要原因之一;然而,仍然有为 GU 的预防或治疗的有效的药的缺乏。在这研究,我们在老鼠把保护的效果和 kaempferol (KAE ) 的可能的机制与尖锐导致乙醇的损害作比较到胃的 mucosa。Fasted 老鼠口头上地被给车辆(0.9% 盐) , omeprazole (20 mg/kg ) ,或为在在由有绝对乙醇(10 ml/kg ) 的挑战的 GU 模型的建立以前的不同试验性的集合的 1 h 的 KAE (40, 80,或 160 mg/kg ) 。动物是 euthanized 在乙醇吸入以后的 1 h,和他们的血浆和胃纸巾服从于进一步的检查。宏观、显微镜的损害,和免疫学的和生物化学的参数被观察。发炎的效果用下列指示物被调查:肿瘤坏死因素 --(TNF-) , interleukin-1 (IL-1 ) , IL-6, myeloperoxidase (军邮局) ,和氮的氧化物(没有) 。结果证明 KAE 显著地减少了溃疡索引,增加了预防索引,完全保护了 mucosa 免受损害,和保存胃的 mucosal glycoprotein 的伤害。KAE 减少了军邮局活动和支持 inflammatory cytokine (TNF- ,和 IL-1 ) 铺平,并且没改进层次。KAE 的 gastroprotective 活动可能被归因于胃的有粘液的 glycoproteins 层次的保藏,这样由禁止嗜中性的累积和军邮局活动,调整支持 inflammatory cytokines 的层次,并且不改进生产。 | Qinchen Li Xinxin Hu Yanhan Xuan Jianghua Ying Yujia Fei Jielu Rong Yong Zhang Jian Zhang Chunyan Liu Zheng Liu | 2018 | Acta Biochimica et Biophysica Sinica2018，50，3： | 18 |
| 2 | Molecular characteristics of the omp A gene of serotype B Chlamydia trachomatis in Qinghai Tibetan primary school students显示文摘To study the molecular characteristics of Chlamydia trachomatis, the major outer membrane protein gene(omp A) of C. trachomatis from primary school students with trachoma residing in the Qinghai Tibetan area was sequenced and compared with the same serotype in Gen Bank. In Jianshetang Primary School and Galeng Central Primary School in the Galeng Tibetan Township of Qinghai Haidong Sala Autonomous County, scraped samples were collected from the upper tarsal conjunctiva and lower conjunctival sac of both eyes of 45 students with trachoma, stored at 4°C, and transported to Beijing Tongren Hospital by air within 24 h. The samples were screened for C. trachomatis by real-time PCR. The omp A gene from the C. trachomatis-positive samples was amplified by nested PCR. The serotype was confirmed by National Center for Biotechnology Information(NCBI) BLAST search and homology analysis. The entire omp A gene sequence was compared with the corresponding gene sequences of serotype B strains available in Gen Bank. Of the 45 students aged 6–13 years with trachoma, 26 C. trachomatis-positive students were identified by the initial real-time PCR screening(average age,(9.09±1.63) years; sex ratio, 1.0), accounting for 57.78%(26/45). The cycle threshold values for real-time PCR were 16.79–37.77. Half(13/26) of C. trachomatis-positive students had a bacterial copy number of >105. The compliance rate of the omp A gene sequences with the C. trachomatis serotype B strains in Gen Bank was up to 99%. Two novel genetic mutations were found when the omp A gene was compared with those of the 11 serotype B strains in Gen Bank. The two non-synonymous mutations were located at(i) position 271 in the second constant domain, an adenine(A) to guanine(G) substitution(ACT?GCT), changing the amino acid at position 91 from threonine to alanine(Thr?Ala) in all 26 strains; and(ii) position 887 in the fourth variable domain, a cytosine(C) to thymine(T) substitution(GCA?GTA), changing the amino acid at residue 296 from alanine to valine(Ala?Val) in four of the 26 strains. Six mutations were identified relative to ATCC VR-573. The strains could be divided into two gene clusters according to the mutation at nucleotide position 887: CQZ-1(China Qinghai Tibetan-1) and CQZ-2(China Qinghai Tibetan-2). We thus detected two novel serotype B mutant strains of C. trachomatis among study subjects with trachoma. | Xue Li Shaoya Zhang Qingfeng Liang Mei Wang Ailian Hu Xiuyuan Li Benshan Yang Mingxin Zhang Ningli Wang Xinxin Lu | 2016 | Science China(Life Sciences)2016，59，6： | 6 |
| 3 | The spatial pattern of farming and factors influencing it during the Peiligang culture period in the middle Yellow River valley, China显示文摘The Peiligang culture(ca.7000–5000 cal BC)is the earliest Neolithic culture in the middle Yellow River valley and represents the emergence of cereal farming in the region[1].It has long been considered representative of millet farming[2],which requires a dry field,a certain amount of rainfall,and minimal crop management,but new records reveal that mixed farming of millet and rice(Oryza sp.)took place during this period[3].Therefore,it is possible | Can Wang Houyuan Lu Wanfa Gu Naiqin Wu Jianping Zhang Xinxin Zuo Fengjiang Li Daojing Wang Yajie Dong Songzhi Wang Yanfeng Liu Yingjian Bao Yayi Hu | 2017 | Science Bulletin2017，62，23： | 6 |
| 4 | In vitro and in vivo activity of D-serine in combination with β-lactam antibiotics against methicillin-resistant Staphylococcus aureus显示文摘As D-amino acids play important roles in the physiological metabolism of bacteria,combination of D-amino acids with antibiotics may provide synergistic antibacterial activity. The aim of the study was to evaluate in vitro and in vivo activity of D-serine alone and in combination withβ-lactams against methicillin-resistant Staphylococcus aureus(MRSA) strains, and to explore the possible sensitization mechanisms. The activity of D-serine, β-lactams alone and in combinations was evaluated both in vitro by standard MICs, time–kill curves and checkerboard assays, and in vivo by murine systemic infection model as well as neutropenic thigh infection model. An in vitro synergistic effect was demonstrated with the combination of D-serine and β-lactams against MRSA standard and clinical strains.Importantly, the combinations enhanced the therapeutic efficacy in the animal models as compared toβ-lactam alone groups. Initial mechanism study suggested possible revision of D-alanine-D-alanine residue to D-alanine-D-serine in peptidoglycan by adding of D-alanine in the medium, which may cause decreased affinity to PBPs during transpeptidation. In conclusion, D-serine had synergistic activity in combination with β-lactams against MRSA strains both in vitro and in vivo. Considering the relatively good safety of D-serine alone or in combination with β-lactams, D-serine is worth following up as new anti-MRSA infection strategies. | Qing Wang Yuemeng Lv Jing Pang Xue Li Xi Lu Xiukun Wang Xinxin Hu Tongying Nie Xinyi Yang Yan Q.Xiong Jiandong Jiang Congran Li Xuefu You | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 6 |
| 5 | The chromosome-level Hemerocallis citrina Borani genome provides new insights into the rutin biosynthesis and the lack of colchicine显示文摘Hemerocallis citrina Borani(huang hua cai in Chinese)is an important horticultural crop whose flower buds are widely consumed as a delicious vegetable in Asia.Here we assembled a high-quality reference genome of H.citrina using single-molecule sequencing and Hi-C technologies.The genome assembly was 3.77 Gb and consisted of 3183 contigs with a contig N50 of 2.09 Mb,which were further clustered into 11 pseudochromosomes.A larger portion(3.25 Gb or 86.20%)was annotated as a repetitive content and 54,295 protein-coding genes were annotated in the genome.Genome evolution analysis showed that H.citrina experienced a recent whole-genome duplication(WGD)event at~15.73 million years ago(Mya),which was the main factor leading to many multiple copies of orthologous genes.We used this reference genome to predict 20 genes involved in the rutin biosynthesis pathway.Moreover,our metabolomics data revealed neither colchicine nor its precursors in H.citrina,challenging the long-standing belief that this alkaloid causes poisoning by the plant.The results of our disruptive research are further substantiated by our genomic finding that H.citrina does not contain any genes involved in colchicine biosynthesis.The high-quality genome lays a solid foundation for genetic research and molecular breeding of H.citrina. | Zhixing Qing Jinghong Liu Xinxin Yi Xiubin Liu Guoan Hu Jia Lao Wei He Zihui Yang Xiaoyan Zou Mengshan Sun Peng Huang Jianguo Zeng | 2021 | Horticulture Research2021，8，1： | 6 |
| 6 | Covalent organic frameworks derived hollow structured N-doped noble carbon for asymmetric-electrolyte Zn-air battery显示文摘We report a relatively low-temperature molten salt strategy to prepare hollow structured N-doped noble carbon(h-NNC) with highly desirable features of ultra-large surface area(1957 m^2 g^(-1)) and high graphitization, endowing the h-NNC with high activity toward catalysis of oxygen reduction reaction in acidic medium. The h-NNC is applied as cathode catalyst of an asymmetrical-electrolyte Zn-air battery, which exhibits an open circuit voltage of 2.11 V, a power density up to 270 mW cm^(-2),and an energy density of 1279 W h kg^(-1), behaving advantages over the conventional Zn-air batteries. | Pingwei Cai Xinxin Peng Junheng Huang Jingchun Jia Xiang Hu Zhenhai Wen | 2019 | Science China Chemistry2019，62，3： | 6 |
| 7 | Postoperative chemotherapy with S-1 plus oxaliplatin versus S-1alone in locally advanced gastric cancer(RESCUE-GC study): a protocol for a phase Ⅲ randomized controlled trial显示文摘Background:The ACTS-GC study had shown postoperative adjuvant therapy with S-1 improved survival of patients with locally advanced gastric cancer.Addition of oxaliplatin to S-1 is considered to be acceptable as one of the treatment options for gastric cancer patients after radical gastrectomy with D2 lymph node excision.Methods:We have commenced a randomized phase III trial in December 2016 to evaluate S-1 plus oxaliplatin compared with S-1 alone in the adjuvant setting for locally advanced gastric cancer.A total of 564 patients will be accrued from 13 Chinese institutions in two years.The primary endpoint is 3-year relapse-free survival.The secondary endpoints are 5-year overall survival,proportion of patients who complete the postoperative chemotherapy and incidence of adverse events.Ethic and dissemination:The trial has been approved by the institutional review board of each participating institution and it was activated on December,2016.The enrollment will be finished in December,2018.Patient’s follow-up will be ended until December,2023.Trial registration:Clinical Trials.gov,identifier:NCT02867839.Registered on August 4,2016. | Xiang Hu Lin Chen Yian Du Biao Fan Zhaode Bu Xin Wang Yingjiang Ye Zhongtao Zhang Gang Xiao Fei Li Qingsi He Guoli Li Xian Shen Bin Xiong Liming Zhu Jiwei Liui Lian Liu Tao Wu Jing Zhou Jun Zhang Gang Zhao Xulin Wang Pin Liang Xinxin Wang Yan Zhang Xiaojiang Wu Ji Zhang Xin Ji Xianglong Zong Tao Fu Ziyu Jia Jiafu Ji | 2017 | Chinese Journal of Cancer Research2017，29，2： | 5 |
| 8 | The histone methyltransferase EZH2 primes the early differentiation of follicular helper T cells during acute viral infection显示文摘Epigenetic modifications to histones dictate the differentiation of naïve CD4^(+) T cells into different subsets of effector T helper(TH)cells.The histone methyltransferase enhancer of zeste homolog 2(EZH2)has been implicated in the mechanism regulating the differentiation of TH1,TH2 and regulatory T(Treg)cells.However,whether and how EZH2 regulates follicular helper T(TFH)cell differentiation remain unknown.Using a mouse model of acute lymphocytic choriomeningitis virus(LCMV)infection,we observed abundant EZH2 expression and associated H3K27me3 modifications preferentially in the early committed virus-specific TFH cells compared to those in TH1 cells.Ablation of EZH2 in LCMV-specific CD4^(+) T cells leads to a selective impairment of early TFH cell fate commitment,but not late TFH differentiation or memory TFH maintenance.Mechanistically,EZH2 specifically stabilizes the chromatin accessibility of a cluster of genes that are important for TFH fate commitment,particularly B cell lymphoma 6(Bcl6),and thus directs TFH cell commitment.Therefore,we identified the chromatin-modifying enzyme EZH2 as a novel regulator of early TFH differentiation during acute viral infection. | Xiangyu Chen Guoshuai Cao Jialin Wu Xinxin Wang Zhiwei Pan Jianbao Gao Qin Tian Lifan Xu Zhirong Li Yaxing Hao Qizhao Huang Pengcheng Wang Minglu Xiao Luoyingzi Xie Shupei Tang Zhenyu Liu Li Hu Jianfang Tang Ran He Li Wang Xinyuan Zhou Yuzhang Wu Mengjie Chen Beicheng Sun Bo Zhu Jun Huang Lilin Ye | 2020 | Cellular & Molecular Immunology2020，17，3： | 4 |
| 9 | Etiological characteristics of chlamydia trachoma conjunctivitis of Primary Boarding School students in the Qinghai Tibetan area显示文摘The aim of this study was to investigate the etiological characteristics of Chlamydia trachomatis conjunctivitis among resident students at primary schools in the Qinghai Tibetan area in order to understand the distribution of C. trachomatis and other pathogenic microorganisms, to detect the isolation rate of infectious pathogens, and to provide an evidence for further targeted efforts in the prevent of sporadic trachoma efforts. From two primary schools in Qinghai Province, ocular samples from 35 students who were clinically diagnosed as trachoma cases and 60 normal controls were obtained by swabbing their upper eyelids and lower conjunctival sacs. Samples were preserved at 4°C and airlifted to Beijing Tongren Hospital within 24 h. Real-time polymerase chain reaction(RT-PCR) was used to screen for C. trachomatis, and nested PCR was used to amplify a fragment of the omp A gene for serotype confirmation. Bacterial cultivation and sensitivity tests were conducted based on the 2015 version of the Clinical and Laboratory Standards Institute. Adenovirus, herpes simplex virus, cytomegalovirus, and Epstein-Barr virus were screened by RT-PCR. Among the 35 students with trachoma, 8 came from the Jianshetang Primary School and 27 came from the Central Primary School. Two novel C. trachomatis B serotypes(Gen Bank accession numbers KU737520 and KU737521) were detected based on a sequence analysis of the omp A gene. Single C. trachomatis infections accounted for 42.86%(9/21) of the cases, and infections with multiple bacteria, particularly Haemophilus influenzae, Staphylococcus aureus, Moraxella catarrhalis, and Streptococcus pneumoniae, accounted for the remaining 57.14%(12/21). Of the 14 C. trachomatis-negative samples, one was positive for adenoviral infection(serotype D) and 13 were positive for bacterial infections(H. influenzae, M. catarrhalis, S. pneumoniae, S. aureus, streptococci other than S. pneumoniae, Staphylococcus epidermidis, Corynebacterium, and Arthrobacterium). In addition to C. trachomatis, the other bacteria and virus that were detected in the boarding students of primary schools in the Qinghai Tibetan area should be emphasized in trachoma prevention and control. | Mei Wang Xinxin Lu Ailian Hu Mingxin Zhang Xue Li Shijing Deng Ningli Wang | 2016 | Science China(Life Sciences)2016，59，6： | 4 |
| 10 | PARAMETERS DETERMINATION METHOD OF PHASE-SPACE RECONSTRUCTION BASED ON DIFFERENTIAL ENTROPY RATIO AND RBF NEURAL NETWORK显示文摘Phase space reconstruction is the first step of recognizing the chaotic time series.On the basis of differential entropy ratio method,the embedding dimension opt m and time delay t are optimal for the state space reconstruction could be determined.But they are not the optimal parameters accepted for prediction.This study proposes an improved method based on the differential entropy ratio and Radial Basis Function(RBF)neural network to estimate the embedding dimension m and the time delay t,which have both optimal characteristics of the state space reconstruction and the prediction.Simulating experiments of Lorenz system and Doffing system show that the original phase space could be reconstructed from the time series effectively,and both the prediction accuracy and prediction length are improved greatly. | Zhang Shuqing Hu Yongtao Bao Hongyan Li Xinxin | 2014 | Journal of Electronics(China)2014，31，1： | 4 |
| 11 | Molecular Characteristics and Phylogenetic Analysis of N Gene of Human Derived Rabies Virus显示文摘Objective To investigate the relationship between the molecular characteristics and phylogenetic evolution of rabies N gene.Methods Saliva samples were collected from rabies cases,and RT-PCR was used to amplify the N gene of rabies virus with the specific primers.The amplifying product of RT-PCR was cloned to pUCm-T vector and transformed into E.coli XL1-Blue and then the blue-white selection,PCR screening and gene sequencing were carried out to identify the positive clones.Finally,ExPASy and other bioinformatics softwares were used to analyze and predict the structure and biological characteristics of the N genome.Results The amplification product of RT-PCR was 1 353 bp,the recombinant plasmid pUCm-T/N was constructed,the whole length of the N gene open reading frame was composed of 1 353 nucleotide residues to code 450 amino acids (20 kinds),the accession number submitted to the Genbank was HM756692,its sequence homology of nucleotides and amino acids compared with the vaccine strain CTN-1-V was 90% and 99% respectively.The evolutionary analysis showed that the isolated strain belonged to genotype I with certain geographic regionality.Conclusion The characteristics investigation and bioinformatics analysis of Hunan0806 N gene will provide fundament data to reveal the significance of the N gene characteristics for rabies epidemiology and its prevention & control. | CAI Liang TAO XiaoYan LIU YunZhi ZHANG Hong GAO LiDong HU ShiXiong LIU FuQiang LI Hao SHEN XinXin LIU JiaHui WANG ShiQing TANG Qing | 2011 | Biomedical and Environmental Sciences2011，24，4： | 4 |
| 12 | Study of infectious conjunctivitis among children in rural areas of Qinghai province显示文摘Infectious conjunctivitis is no longer a common eye disease in cities and developed areas, but in remote mountainous regions of China where living conditions are relatively under-developed, the occurrence of infectious conjunctivitis remains a public health issue. Here, we evaluate the ratio of infectious conjunctivitis among primary school students in the rural areas of Xunhua, Huangyuan, and Huzhu Counties in the Qinghai province of China, and report the microorganisms involved in causing infection. Of the 765 eligible primary school students, 694 participated to this study(a response rateof 90.7%), 381 boys and 313 girls, with a mean age of 7.52 years(range 7–9 years). A clinical diagnosis of infectious conjunctivitis was returned for 238 of the 694 children examined. The ratio of infectious conjunctivitis in Xunhua County(46.64%) was higher compared to that in Huangyuan(26.67%; P=0.03) and Huzhu Counties(23.61%; P=0.02). The leading cause of conjunctivitis was bacterial, followed by chlamydial and viral. Our results show that there is a high ratio of infectious conjunctivitis among rural primary school students in Qinghai province. | Qingfeng Liang Xinxin Lu Mei Wang Lei Tian Antoine Labbé Ailian Hu | 2016 | Science China(Life Sciences)2016，59，6： | 3 |
| 13 | Puromycin A, B and C, cryptic nucleosides identified from Streptomyces alboniger NRRL B-1832 by PPtase-based activation显示文摘Natural product discovery is pivot for drug development,however,this endeavor is often challenged by the wide inactivation or silence of natural products biosynthetic pathways.We recently developed a highly efficient approach to activate cryptic/silenced biosynthetic pathways through augmentation of the phosphopantetheinylation of carrier proteins.By applying this approach in the Streptomyces alboniger NRRL B-1832,we herein identified three cryptic nucleosides products,including one known puromycin A and two new derivatives(puromycin B and C).The biosynthesis of these products doesn't require the involvement of carrier protein,indicating the phosphopantetheinyl transferase(PPtase)indeed plays a fundamental regulatory role in metabolites biosynthesis.These results demonstrate that the PPtasebased approach have a much broader effective scope than the previously assumed carrier proteininvolving pathways,which will benefit future natural products discovery and biosynthetic studies. | Xiaoli Yan Benyin Zhang Wenya Tian Qi Dai Xiaoqin Zheng Ke Hu Xinxin Liu Zixin Deng Xudong Qu | 2018 | Synthetic and Systems Biotechnology2018，3，1： | 3 |
| 14 | An insight into failure mechanism of NASICON-structured Na3V2(PO4)3 in hybrid aqueous rechargeable battery显示文摘NASICON (Na-super-ionic-conductors)-structured materials have attracted extensive research interest due to their great application potential in secondary batteries. However, the mechanism of capacity fading for NASICON-structured electrode materials has been rarely studied. In this paper, we synthesized the NASICON-structured Na3V2(PO4)3/C composite by simple sol-gel and high-temperature solid-phase method and investigated its electrochemical performance in Na-Zn hybrid aqueous rechargeable batteries. After characterizing the structure, morphology and composition variations as well as the interfacial resistance changes of Na3V2(PO4)3/C cathode during cycling, we propose a mechanical and interfacial degradation mechanism for capacity fading of NASICON-structured Na3V2(PO4)3/C in Na-Zn hybrid aqueous rechargeable batteries. This work will shed light on enhancing the mechanical and in terfacial stability of NASICON-structured Na3V2(PO4)3/C in Na-Zn hybrid aqueous rechargeable batteries. | Xinxin Zhang Jun Ma Pu Hu Bingbing Chen Chenglong Lu Xinhong Zhou Pengxian Han Lihua Chen Guanglei Cui | 2019 | Journal of Energy Chemistry2019，28，5： | 3 |
| 15 | Scheme on Cross-Domain Identity Authentication Based on Group Signature for Cloud Computing显示文摘In the cloud computing, different cloud service providers are often in different trust domains. As the traditional identity authentication mode cannot be applied to the cloud computing, the cross-domain identity authentication mechanism is needed to solve the identity authentication problem in the cloud computing. In view of the security problems in cloud computing, a cross-domain identity authentication scheme based on group signature is proposed. This scheme introduces a group of cloud service providers and users who are located in different trust domains. Any member of the group can generate the signature on behalf of the whole group, making the user access the cloud service provider in the case of privacy security. At the same time, with traceability it can track illegal operation of illegal users. In addition, the scheme uses the Chinese Remainder Theorem to integrate the message, and it can control the length of the data in the calculation process, simplifying the calculation process. It also realizes the join and revocation of group members without changing the key of other legitimate group members, and the maintenance cost of authentication schemes is low. The results show that the scheme has the advantages of anonymity, anti-counterfeit, traceability, anti-joint attack and so on. It can not only realize tracking function under the condition of guaranteeing user's privacy, but can also simplify the authentication calculation process to improve the efficiency of the cross domain identity authentication, and its performance is more suitable for large-scale cloud computing environment. | YANG Yanyan HU Mingsheng KONG Shan GONG Bei LIU Xinxin | 2019 | Wuhan University Journal of Natural Sciences2019，24，2： | 3 |
| 16 | Anthropogenic Effect on Deposition Dynamics of Lake Sediments Based on ^(137)Cs and ^(210)Pb_(ex) Techniques in Jiuzhaigou National Nature Reserve,China显示文摘Radionuclide dating techniques characterized by137Cs and210Pbex have recently been applied in the study of lake sediments around the world.In this study,a chronological series of sediment cores was established based on137Cs and210Pbex analyses along with the evaluation of sediment properties,such as particle size distribution,total organic carbon(TOC),carbonate content,and acid-insoluble residue,to study sediment accumulation rates,sediment sources,and responses to human activities in the Jiuzhaigou National Nature Reserve in southwestern China.In terms of the particle size distribution of sediments,silt content was the highest,and clay and sand contents were relatively low.The sediments displayed high TOC contents because of the significant amounts of vegetation grown in the lakes.The carbonate content was also high due to the overall geological background of carbonates in Jiuzhaigou.Carbonate content tended to decline from top to bottom in the sediment cores,whereas the acid-insoluble residue tended to increase.These results suggested that the depth variation of the environmental parameters of the sediments in two lakes in Jiuzhaigou would correspond to each other.The results indicated that the sediment rate of Jiuzhaigou was generally high with strong siltation,indicating that serious soil loss was induced by intensive human activities in the basin over the past decades.The increases in the mass accumulation rate,contents of acid-insoluble residue,and mean particle size during the periods of 1840–1900s,late 1930s–early 1950s,1966–1978,and2003–2006 revealed the occurrence of severe soil and water loss as a result of extensive agricultural expansion,large-scale deforestation,and road construction in Jiuzhaigou.The deposition rate and the properties of lacustrine sediments could reflect the significant impact of human activities on lake sedimentation during Jiuzhaigou′s history. | LIANG Kangkang HU Xinxin LI Shiguang HUANG Chengmin TANG Ya | 2014 | Chinese Geographical Science2014，24，2： | 3 |
| 17 | A review on the electric vehicle routing problems: Variants and algorithms显示文摘Over the past decade,electric vehicles(EVs)have been considered in a growing number of models and methods for vehicle routing problems(VRPs).This study presents a comprehensive survey of EV routing problems and their many variants.We only consider the problems in which each vehicle may visit multiple vertices and be recharged during the trip.The related literature can be roughly divided into nine classes:Electric traveling salesman problem,green VRP,electric VRP,mixed electric VRP,electric location routing problem,hybrid electric VRP,electric dial-a-ride problem,electric two-echelon VRP,and electric pickup and delivery problem.For each of these nine classes,we focus on reviewing the settings of problem variants and the algorithms used to obtain their solutions. | Hu QIN Xinxin SU Teng REN Zhixing LUO | 2021 | Frontiers of Engineering Management2021，8，3： | 3 |
| 18 | Genetic basis of high level aminoglycoside resistance in Acinetobacter baumannii from Beijing,China显示文摘The objective of this study was to investigate the genetic basis of high level aminoglycoside resistance in Acinetobacter baumannii clinical isolates from Beijing,China.173 A.baumannii clinical isolates from hospitals in Beijing from 2006 to 2009 were first subjected to high level aminoglycoside resistance(HLAR,MIC to gentamicin and amikacin>512 mg/mL)phenotype selection by broth microdilution method.The strains were then subjected to genetic basis analysis by PCR detection of the aminoglycoside modifying enzyme genes(aac(3)-Ⅰ,aac(3)-Ⅱc,aac(60)-Ⅰb,aac(60)-Ⅱ,aph(4)-Ⅰa,aph(30)-Ⅰ,aph(30)-Ⅱb,aph(30)-Ⅲa,aph(30)-Ⅵa,aph(2″)-Ⅰb,aph(2″)-Ⅰc,aph(2″)-Ⅰd,ant(2″)-Ⅰa,ant(3″)-Ⅰand ant(40)-Ⅰa)and the 16S rRNA methylase genes(armA,rmtB and rmtC).Correlation analysis between the presence of aminoglycoside resistance gene and HLAR phenotype were performed by SPSS.Totally 102(58.96%)HLAR isolates were selected.The HLAR rates for year 2006,2007,2008 and 2009 were 52.63%,65.22%,51.11%and 70.83%,respectively.Five modifying enzyme genes(aac(3)-Ⅰ,detection rate of 65.69%;aac(60)-Ⅰb,detection rate of 45.10%;aph(30)-Ⅰ,detection rate of 47.06%;aph(30)-Ⅱb,detection rate of 0.98%;ant(3″)-Ⅰ,detection rate of 95.10%)and one methylase gene(armA,detection rate of 98.04%)were detected in the 102 A.baumannii with aac(3)-Ⅰ+aac(60)-Ⅰ+þant(3″)-Ⅰ+armA(detection rate of 25.49%),aac(3)-Ⅰ+aph(30)-Ⅰ+ant(3″)-Ⅰ+armA(detection rate of 21.57%)and ant(3″)-Ⅰ+armA(detection rate of 12.75%)being the most prevalent gene profiles.The values of chi-square tests showed correlation of armA,ant(3″)-Ⅰ,aac(3)-Ⅰ,aph(30)-Ⅰand aac(60)-Ⅰb with HLAR.armA had significant correlation(contingency coefficient 0.685)and good contingency with HLAR(kappa 0.940).The high rates of HLAR may cause a serious problem for combination therapy of aminoglycoside with β-lactams against A.baumannii infections.As armA was reported to be able to cause high level aminoglycoside resistance to most of the clinical important aminoglycosides(gentamicin,amikacin,tobramycin,etc),the function of aminoglycoside modifying enzyme gene(s)in A.baumannii carrying armA deserves further investigation. | Lu Nie Yuemeng Lv Min Yuan Xinxin Hu Tongying Nie Xinyi Yang Guoqing Li Jing Pang Jingpu Zhang Congran Li Xiukun Wang Xuefu You | 2014 | Acta Pharmaceutica Sinica B2014，4，4： | 3 |
| 19 | Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert显示文摘Three hundred and twenty endophytic actinobacterial strains were isolated from psammophytes collected from Taklamakan Desert and identified. Among them, three strains already had been identified as new species of two genera and sixteen isolates showed relatively low 16 S rRNA similarities < 98.6% to validly described species. Seventy-five of the isolates were selected as representative strains to screen antibacterial activity and mechanism. Forty-seven strains showed antagonistic activity against at least one of the indicator bacteria. Two Streptomyces strains produced bioactive compounds inducing DNA damage, and two Streptomyces strains produced bioactive compounds with inhibitory activity on protein biosynthesis. Notably, the strain Streptomyces sp. 8P21H-1 that demonstrated both strong antibacterial activity and inhibitory activity on protein biosynthesis was prioritized for exploring new antibiotics.Under the strategy of integrating genetics-based discovery program and MS/MS-based molecular networking, two new streptogramin-type antibiotics, i.e., acetyl-griseoviridin and desulphurizing griseoviridin, along with known griseoviridin, were isolated from the culture broth of strain 8P21H-1. Their chemical structures were determined by HR-MS, and 1D and 2D NMR. Desulphurizing griseoviridin and griseoviridin exhibited antibacterial activities by inhibiting translation. | Ting Wang Feina Li Qinpei Lu Gang Wu Zhongke Jiang Shaowei Liu Xugela Habden Elizaveta A.Razumova Ilya A.Osterman Petr V.Sergiev Olga A.Dontsova Xinxin Hu Xuefu You Chenghang Sun | 2021 | Journal of Pharmaceutical Analysis2021，11，2： | 2 |
| 20 | Human embryonic stem cells contribute to embryonic and extraembryonic lineages in mouse embryos upon inhibition of apoptosis显示文摘 | Wei Li Baoyang Hu XuepengWang Tianda Li Tongtong Cui Dawei Yu Chao Liu Liyuan Jiang Guihai Feng Lei wang Rui Fu Xinxin Zhang Jie Hao Yukai Wang Liu Wang Qi Zhou | 2018 | Cell Research2018，28，1： | 2 |
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