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| 1 | Synergistic activity of lawsone methyl ether in combination with some antibiotics and artocarpin against methicillin-resistant Staphylococcus aureus, Candida albicans, and Trychophyton rubrum显示文摘Objective: One appealing strategy to overcome and prevent resistant problem is the use of combined two or more antibacterial substances. Lawsone methyl ether(LME) is the naphthoquinone found in the leaves of Impatiens balsamina. The objective of this study is to determine the interaction of LME with some antibiotics(ampicillin, tetracycline, norfloxacin, and clotrimazole) and a natural compound, artocarpin against methicillin-resistant Staphylococcus aureus(MRSA), Candida albicans, and Trychophyton rubrum.Methods: A broth microdilution method was used to determine the minimum inhibition concentration(MIC). Synergistic effects were evaluated at their own MIC using the checkerboard method and time-kill assay.Results: LME showed moderate antibacterial activity against MRSA with MIC value of 15.6 μg/mL, and exhibited strong antifungal activities against T. rubrum and C. albicans with MIC values of 7.8 and 3.9 μg/m L,respectively. The interaction of LME with the natural compound artocarpin against MRSA produced a synergy with fractional inhibitory concentration index(FICI) value of 0.31, while the combination of LME and clotrimazole exhibited synergy against C. albicans and T. rubrum with FICI values of 0.38 and 0.24, respectively. The time-kill assays confirmed that the compounds in combination enhanced their antimicrobial activities against the resistant microorganisms with different degrees.Conclusion: LME in combination with clotrimazole exhibited synergy effect against C. albicans and T.rubrum. In combination with artocarpin, it showed synergy effect against MRSA. | Pharkphoom Panichayupakaranant Abdi Wira Septama Akarawat Sinviratpong | 2019 | Chinese Herbal Medicines2019，11，3： | 11 |
| 2 | Anti-allergic principles of Rhinacanthus nasutus leaves显示文摘 | Supinya Tewtrakul Pimpimon Tansakul Pharkphoom Panichayupakaranant | 2009 | Phytomedicine2009，，10： | 1 |
| 3 | Topical anti-inflammatory and analgesic activities of standardized pomegranate rind extract in comparison with its marker compound ellagic acid in vivo显示文摘 | Jiao Mo Pharkphoom Panichayupakaranant Nattha Kaewnopparat Anupong Nitiruangjaras Wantana Reanmongkol | 2013 | Journal of Ethnopharmacology2013，，3： | 1 |
| 4 | Naphthoquinones and coumarins from Impatiens balsamina root cultures显示文摘 | PHARKPHOOM P HIROSHI N WANCHAI DE-E | 1995 | Phytochemistry1995，40，4： | 1 |
| 5 | A new biscoumarin from Impatiens balsamina root cultures显示文摘 | PHARKPHOOM P | 1998 | Plant Med1998，，64： | 1 |
| 6 | A New Biscoumarin from Impatiens balsamina Root Cultures显示文摘 | Pharkphoom Panichayupakaranant Hiroshi Noguchi Wanchai De-Eknamkul | 1998 | Planta Med1998，，08： | 1 |
| 7 | High water-soluble curcuminoids-rich extract regulates osteogenic differentiation of MC3T3-E1 cells:Involvement of Wnt/β-catenin and BMP signaling pathway显示文摘Objective:The present study aimed to evaluate the effect of a high water-soluble curcuminoids-rich extract(CRE)in a solid dispersion form(CRE-SD)using polyvinylpyrrolidone K30 on osteogenic induction of MC3T3-E1 cells.Methods:CRE was pre-purified using a microwave assisted extraction couple with a Diaion;HP-20 column chromatography.The osteoblastic cell proliferation and differentiation potentials of CRE-SD in MC3T3-E1 cells were tested by cell viability,alkaline phosphatase(ALP)activity,and Alizarin red S activity assays.The m RNA expressions of osteoblast-specific genes and underline mechanisms were assessed by a real time PCR and western blot analysis.Results:CRE-SD 50μg/m L increased alkaline phosphatase(ALP)activity,an early differentiation marker of osteoblasts in both MC3T3-E1 cells and non-osteogenic mouse pluripotent cell line,C3H10T1/2,indicating the action of CRE-SD was not cell-type specific.Alizarin red S activity showed a significant amount of calcium deposition in cells treated with CRE-SD.CRE-SD also upregulated the m RNA expression levels of transcription factors that favor osteoblast differentiation including Bmp-2,Runx2 and Collagen 1 a,in a dose dependent manner.Western blot analysis revealed that noggin attenuated CRE-SD-promoted expressions of Bmp-2 and Runx2 proteins.si RNA mediated blocking of Wnt/β-catenin signaling pathway also annulled the influence of CRE-SD,indicating Wnt/β-catenin dependent activity.Inhibition of the different signaling pathways abolished the influence of CRE-SD on ALP activity,confirming that CRE-SD induced MC3T3-E1 cells into osteoblasts through Wnt/β-catenin and BMP signaling pathway.Conclusion:These results collectively demonstrate that CRE-SD may be a potential therapeutic agent for the treatment of osteoporosis. | Yutthana Pengjam Nurul Syazwani Jakkapong Inchai Amornkan Numit Thanintorn Yodthong Thanawat Pitakpornpreecha Pharkphoom Panichayupakaranant | 2021 | Chinese Herbal Medicines2021，13，4： | 1 |
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