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| 1 | Pyroptosis: mechanisms and diseases显示文摘Currently,pyroptosis has received more and more attention because of its association with innate immunity and disease.The research scope of pyroptosis has expanded with the discovery of the gasdermin family.A great deal of evidence shows that pyroptosis can affect the development of tumors.The relationship between pyroptosis and tumors is diverse in different tissues and genetic backgrounds.In this review,we provide basic knowledge of pyroptosis,explain the relationship between pyroptosis and tumors,and focus on the significance of pyroptosis in tumor treatment.In addition,we further summarize the possibility of pyroptosis as a potential tumor treatment strategy and describe the side effects of radiotherapy and chemotherapy caused by pyroptosis.In brief,pyroptosis is a double-edged sword for tumors.The rational use of this dual effect will help us further explore the formation and development of tumors,and provide ideas for patients to develop new drugs based on pyroptosis. | Pian Yu Xu Zhang Nian Liu Ling Tang Cong Peng Xiang Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，4： | 26 |
| 2 | Eukaryotic elongation factor-2 kinase regulates the cross-talk between autophagy and pyroptosis in doxorubicin-treated human melanoma cells in vitro显示文摘Eukaryotic elongation factor-2 kinase (eEF-2K), a negative regulator of protein synthesis, has been shown to play an important role in modulating autophagy and apoptosis in tumor cells under various stresses. In this study, we investigated the regulatory role of eEF-2K in pyroptosis (a new form of programmed necrosis) in doxorubicin-treated human melanoma cells. We found that doxorubicin (0.5-5 μmol/L) induced pyroptosis in melanoma cell lines SK-MEL-5, SK-MEL-28, and A-375 with high expression of DFNA5, but not in human breast cancer cell line MCF-7 with little expression of DFNA5. On the other hand, doxorubicin treatment activated autophagy in the melanoma cells;inhibition of autophagy by transfecting the cells with siRNA targeting Beclin1 or by pretreatment with chloroquine (20 μmol/L) significantly augmented pyroptosis, thus sensitizing the melanoma cells to doxorubicin. We further demonstrated that doxorubicin treatment activated eEF-2K in the melanoma cells, and silencing of eEF-2K blunted autophagic responses, but promoted doxorubicin-induced pyroptotic cell death. Taken together, the above results demonstrate that eEF-2K dictates the cross-talk between pyroptosis and autophagy in doxorubicin-treated human melanoma cells;suppression of eEF-2K results in inhibiting autophagy and augmenting pyroptosis, thus modulating the sensitivity of melanoma cells to doxorubicin, suggesting that targeting eEF-2K may reinforce the antitumor ef?cacy of doxorubicin, offering a new insight into tumor chemotherapy. | Pian Yu Hai-yan Wang Min Tian Ao-xue Li Xi-sha Chen Xin-luan Wang Yi Zhang Yan Cheng | 2019 | Acta Pharmacologica Sinica2019，40，9： | 19 |
| 3 | Suppression of eEF-2K-mediated autophagy enhances the cytotoxicity of raddeanin A against human breast cancer cells in vitro显示文摘最近的证据显示出那 raddeanin A (RA ) ,从海葵 raddeana Regel 提取的 oleanane 类型 triterpenoid saponin,在 vitro 并且在 vivo 对癌症房间施加显著 cytotoxicity。另外, RA 也被发现了在人的胃的癌症房间激活 autophagy。在这研究,我们调查了在 vitro 在人的乳癌房间象在导致 RA 的 autophagy 和它的 cytotoxicity 之间的关系一样位于导致 RA 的 autophagy 下面的分子的机制。有 RA (2-8 mol/L ) 的治疗 dose-dependently 提高了 autophagy,在乳癌房间线 T47D 由增加的 LC3 层次证实了 MCF-7 和 MDA-MB-231。而且,表明小径的 Akt-mTOR-eEF-2K 被表明在 3 根乳癌房间线涉及 autophagy 的导致 RA 的激活。有 RA (2-10 mol/L ) 的治疗 dose-dependently 在 3 根乳癌房间线导致了 apoptosis。有 autophagy 禁止者 chloroquine 的预告的处理(CQ, 20 mol/L ) 经由支持 apoptosis 的显著地提高的引起 RA 的 cytotoxicity。在结论,我们的结果建议 modulating autophagy 能对人的乳癌房间增强 RA 的 cytotoxicity。 | Yi-di GUAN Shi-long JIANG Pian YU Mei WEN Yi ZHANG Song-shu XIAO Xiao-jun XU Yan CHENG | 2018 | Acta Pharmacologica Sinica2018，39，4： | 6 |
| 4 | Modeling of austenite decomposition in plain carbon steels during hot rolling显示文摘 | ZHANG Yu tuo LI Pian zhong LI Yi yi | | 0，，： | 1 |
| 5 | Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke显示文摘Human dental pulp stem cells(hDPSCs) promote recovery after ischemic stro ke;however,the therapeutic efficacy is limited by the poor survival of transplanted cells.For in vitro expe riments in the present study,we used oxygen-glucose deprivation/reoxygenation in hDPSCs to mimic cell damage induced by ischemia/reperfusion.We found that miRNA-34a-5p(miR-34a) was elevated under oxygen-glucose deprivation/reoxygenation conditions in hDPSCs.Inhibition of miR-34a facilitated the prolife ration and antioxidant capacity and reduced the apoptosis of hDPSCs.Moreove r,dual-luciferase reporter gene assay showed WNT1and SIRT1 as the targets of miR-34a.In miR-34a knockdown cell lines,WNT1 suppression reduced cell prolife ration,and SIRT1 suppression decreased the antioxidant capacity.Togethe r,these results indicated that miR-34a regulates cell prolife ration and antioxidant stress via targeting WNT1 and SIRT1,respectively.For in vivo expe riments,we injected genetically modified hDPSCs(anti34a-hDPSCs) into the brains of mice.We found that anti34a-hDPSCs significantly inhibited apoptosis,reduced cerebral edema and cerebral infarct volume,and improved motor function in mice.This study provides new insights into the molecular mechanism of the cell prolife ration and antioxidant capacity of hDPSCs,and suggests a potential gene that can be targeted to improve the survival rate and efficacy of transplanted hDPSCs in brain after ischemic stroke. | Jianfeng Wang Peibang He Qi Tian Yu Luo Yan He Chengli Liu Pian Gong Yujia Guo Qingsong Ye Mingchang Li | 2023 | Neural Regeneration Research2023，18，9： | 1 |
| 6 | A new perspective in pyroptosis:lifting the veil on GSDMA activation显示文摘Pyroptosis is an inflammatory form of death mediated by gasdermin that recruits immune cells to the site of infection and promotes protective immunity.The gasdermin is a family of pore-forming proteins,including GSDMA(Gsdma1,Gsdma2,Gsdma3),GSDMB,GSDMC(Gsdmc1,Gsdmc2,Gsdmc3,Gsdmc4),GSDMD(Gsdmd),GSDME/DFNA5(Dfna5)and GSDMF/DFNB59/PJVK(Dfnb59)[1,2].Though activation of GSDMB/C/D/E has been gradually illustrated[3],the details of GSDMA activation,particular in epithelial cells such as skin and upper gastrointestinal tract,still remain so far largely elusive. | Pian Yu Xiang Chen Cong Peng | 2023 | Frontiers of Medicine2023，17，3： | 0 |
| 7 | A Rhodamine-Based Sensor for Chromogenic Detection of Cu^(2+) and Fluorescent Detection of Fe^(3+)显示文摘A rhodamine-benzimidazole conjugate(RB) as a probe was investigated.UV-Vis analysis showed that a strong absorption band at 552 nm was formed with the addition of Cu^(2+),while other transition metal ions induced a little more absorption.The absorption value of RB solution at 552 nm has a linear correlation with Cu^(2+) concentration between 35-70 μmol/L;the detection limit reached 6.82×10^(-2) μmol/L,which is lower than the settled limitation for copper in the drinking water(~30 μmol/L) standardized by World Health Organization(WHO).Moreover,FL analysis showed that only Fe^(3+) could induce large fluorescence intensity enhancement at 582 nm;other common metal ions including Cu^(2+) cannot induce the enhancement.There was a good linear correlation between relative fluorescence intensity and Fe^(3+) concentration ranging from 2 μmol/L to 20 μmol/L,and the detection limit reached1.70×10^(-2) μmol/L.The results showed that RB could detect Cu^(2+)and Fe^(3+) simultaneously,with the UV-Vis and fluorescence spectroscopy,respectively. | YU Xianglin QU Hao HU Qihui HOU Kun YAN Yushi WU Dan ZHANG Pian LI Junbo | 2014 | Wuhan University Journal of Natural Sciences2014，19，2： | 0 |
| 8 | Identification of HMGCR as the anticancer target of physapubenolide against melanoma cells by in silico target prediction显示文摘Physapubenolide(PB),a withanolide-type compound extracted from the traditional herb Physalis minima L.,has been demonstrated to exert remarkable cytotoxicity against cancer cells;however,its molecular mechanisms are still unclear.In this study,we demonstrated that PB inhibited cell proliferation and migration in melanoma cells by inducing cell apoptosis.The anticancer activity of PB was further verified in a melanoma xenograft model.To explore the mechanism underlying the anticancer effects of PB,we carried out an in silico target prediction study,which combined three approaches(chemical similarity searching,quantitative structure-activity relationship(QSAR),and molecular docking)to identify the targets of PB,and found that PB likely targets 3-hydroxy-methylglutaryl CoA reductase(HMGCR),the rate-limiting enzyme of the mevalonate pathway,which promotes cancer cell proliferation,migration,and metastasis.We further demonstrated that PB interacted with HMGCR,decreased its protein expression and inhibited the HMGCR/YAP pathway in melanoma cells.In addition,we found that PB could restore vemurafenib sensitivity in vemurafenib-resistant A-375 cells,which was correlated with the downregulation of HMGCR.In conclusion,we demonstrate that PB elicits anticancer action and enhances sensitivity to vemurafenib by targeting HMGCR. | Hai-yan Wang Pian Yu Xi-sha Chen Hui Wei Shi-jie Cao Meng Zhang Yi Zhang Yong-guang Tao Dong-sheng Cao Feng Qiu Yan Cheng | 2022 | Acta Pharmacologica Sinica2022，43，6： | 0 |
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