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| 1 | Mobilization of hematopoietic progenitor cells in patients with liver cirrhosis显示文摘AIM:To test the hypothesis that liver cirrhosis is associated with mobilization of hematopoietic progenitor cells. METHODS:Peripheral blood samples from 72 patients with liver cirrhosis of varying etiology were analyzed by flow cytometry.Identified progenitor cell subsets were immunoselected and used for functional assays in vitro. Plasma levels of stromal cell-derived factor-1(SDF-1) were measured using an enzyme linked immunosorbent assay.RESULTS:Progenitor cells with a CD133 + /CD45 + CD14 + phenotype were observed in 61%of th patients.Between 1%and 26%of the peripheral bloo mononuclear cells(MNCs)displayed this phenotype Furthermore,a distinct population of c-kit + progenito cells(between 1%and 38%of the MNCs)could b detected in 91%of the patients.Additionally,18% of the patients showed a population of progenito cells(between 1%and 68%of the MNCs)that wa characterized by expression of breast cancer resistanc protein-1.Further phenotypic analysis disclosed tha the circulating precursors expressed CXC chemokin receptor 4,the receptor for SDF-1.In line with thi finding,elevated plasma levels of SDF-1 were presen in all patients and were found to correlate with th number of mobilized CD133 + progenitor cells. | Ursula M Gehling Marc Willems Kathleen Schlagner Ralf A Benndorf Maura Dandri Jrg Petersen Martina Sterneck Joerg-Matthias Pollok Dieter K Hossfeld Xavier Rogiers | 2010 | World Journal of Gastroenterology2010，16，2： | 4 |
| 2 | Preclinical activity of a novel multiple tyrosine kinase and aurora kinase inhibitor,ENMD-2076,against multiple myeloma显示文摘 | WANG X J SINN A L POLLOK K | 2010 | Br J Haematol2010，150，3： | 1 |
| 3 | Echoes from childhoodi?mitation in Gilles de la Tourette Syndrome显示文摘 | FinisJ Moczydlowski A Pollok B | 2012 | Mov Disord2012，27，4： | 1 |
| 4 | Identification of diagnostic markers for tuberculosis by proteomic fingerprinting of serum 显示文摘 | Agranoff D Fernandez-Reyes D Papadopoulos MC Rojas SA Herbster M Loosemore A Tarelli E Sheldon J Schwenk A Pollok R Rayner CF Krishna S | 2006 | Lancet2006，368，： | 1 |
| 5 | Preclinical activity of a novel multiple tyrosine kinase and aurora kinase inhibitor, ENMD-2076, against multiple myeloma 显示文摘 | WANG X SINN A L POLLOK K | 2010 | Br J Haematol2010，150，3： | 1 |
| 6 | On the Cramer-Rao bound for polynomial phase signals 显示文摘 | McKilliam R Pollok A | 2014 | Signal Processing2014，95，1： | 1 |
| 7 | Host cell factor requirement for hepatitis C virus enzyme maturation 显示文摘 | Waxman L Whitney M Pollok B A | 2001 | Proc Natl Acad Sci USA2001，98，13： | 1 |
| 8 | Multivisceral resection for locally advanced primary colon and rectal cancer: an analysis of prognostic factors in 201 patients显示文摘 | Lehnert T Methner M Pollok A | 2002 | Ann Surg2002，235，2： | 1 |
| 9 | Preclinical activity of a novel multiple tyrosine kinase and aurora kinase inhibitor, ENMD-2076, against multiple myeloma 显示文摘 | WANG X J SINN A L POLLOK K | 2010 | Br J Haematol2010，150，3： | 1 |
| 10 | On the Cramer - Rao bound for polyno-mial phasesignals显示文摘 | McKilliam R Pollok A | 2014 | Signal Processing2014，95，1： | 1 |
| 11 | Using GFP in FRET-based applications 显示文摘 | Pollok B A Heim R | 1999 | Trends Cell Biol1999，9，2： | 1 |
| 12 | Multivisceral resection for locally advanced primary colon and rectal cancer: an analysis of prognostic factors in 201 patients显示文摘 | Lehnert T Methner M Pollok A | 2002 | Ann Surg2002，235，2： | 1 |
| 13 | Modified cramer-rao bounds for continuous-phase modulated signals显示文摘 | Pollok A McKilliam R | 2014 | IEEE Transactions on Communications2014，23，4： | 1 |
| 14 | Targeting the anti-apoptotie protein c-FLIP for cancer therapy 显示文摘 | Safa A R Pollok K E | 2011 | Cancers2011，3，2： | 1 |
| 15 | How old is too old? In vivo engraftment of human peripheral blood stem cells cryopreserved for up to 18 years-implications for clinical transplantation and stability programs显示文摘BACKGROUND Peripheral blood stem cells(PBSC)are commonly cryopreserved awaiting clinical use for hematopoietic stem cell transplant.Long term cryopreservation is commonly defined as five years or longer,and limited data exists regarding how long PBSC can be cryopreserved and retain the ability to successfully engraft.Clinical programs,stem cell banks,and regulatory and accrediting agencies interested in product stability would benefit from such data.Thus,we assessed recovery and colony forming ability of PBSC following long-term cryopreservation as well as their ability to engraft in NOD/SCID/IL-2 Rγnull(NSG)mice.AIM To investigate the in vivo engraftment potential of long-term cryopreserved PBSC units.METHODS PBSC units which were collected and frozen using validated clinical protocols were obtained for research use from the Cellular Therapy Laboratory at Indiana University Health.These units were thawed in the Cellular Therapy Laboratory using clinical standards of practice,and the pre-freeze and post-thaw characteristics of the units were compared.Progenitor function was assessed using standard colony-forming assays.CD34-selected cells were transplanted into immunodeficient mice to assess stem cell function.RESULTS Ten PBSC units with mean of 17 years in cryopreservation(range 13.6-18.3 years)demonstrated a mean total cell recovery of 88%±12%(range 68%-110%)and post-thaw viability of 69%±17%(range 34%-86%).BFU-E growth was shown in 9 of 10 units and CFU-GM growth in 7 of 10 units post-thaw.Immunodeficient mice were transplanted with CD34-selected cells from four randomly chosen PBSC units.All mice demonstrated long-term engraftment at 12 wk with mean34%±24%human CD45+cells,and differentiation with presence of human CD19+,CD3+and CD33+cells.Harvested bone marrow from all mice demonstrated growth of erythroid and myeloid colonies.CONCLUSION We demonstrated engraftment of clinically-collected and thawed PBSC following cryopreservation up to 18 years in NSG mice,signifying likely successful clinical transplantation of PBSC following long-term cryopreservation. | John Underwood Mahvish Rahim Carijo West Rebecca Britton Elaine Skipworth Vicki Graves Steven Sexton Hillary Harris Dave Schwering Anthony Sinn Karen E Pollok Kent A Robertson W Scott Goebel Kerry M Hege | 2020 | World Journal of Stem Cells2020，12，5： | 0 |
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