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| 1 | Modulation of faecal metagenome in Crohn's disease:Role of microRNAs as biomarkers显示文摘BACKGROUND The gut microbiota plays a key role in the maintenance of intestinal homeostasis and the development and activation of the host immune system. It has been shown that commensal bacterial species can regulate the expression of host genes. 16 S rRNA gene sequencing has shown that the microbiota in inflammatory bowel disease(IBD) is abnormal and characterized by reduced diversity. Micro RNAs(miRNAs) have been explored as biomarkers and therapeutic targets, since they are able to regulate specific genes associated with Crohn's disease(CD). In this work, we aim to investigate the composition of gut microbiota of active treatment-na?ve adult CD patients, with miRNA profile from gut microbiota.AIM To investigate the composition of gut microbiota of active treatment-na?ve adult CD patients, with miRNA profile from gut microbiota.METHODS Patients attending the outpatient clinics at Valme University Hospital without relevant co-morbidities were matched according to age and gender. Faecal samples of newonset CD patients, free of treatment, and healthy controls were collected. Faecal samples were homogenized, and DNA was amplified by PCR using primers directed to the 16 S bacterial rRNA gene. Pyrosequencing was performed using GS-Junior platform. For sequence analysis, MGRAST server with the database Ribosomal Project was used. MiRNA profile and their relative abundance were analyzed by quantitative PCR.RESULTS Microbial community was characterized using 16 S rRNA gene sequencing in 29 samples(n = 13 CD patients, and n = 16 healthy controls). The mean Shannon diversity was higher in the healthy control population compared to CD group(5.5 vs 3.7). A reduction in Firmicutes and an increase in Bacteroidetes were found. Clostridia class was also significantly reduced in CD. Principal components analysis showed a grouping pattern, identified in most of the subjects in both groups, showing a marked difference between control and CD groups. A functional metabolic study showed that a lower metabolism of carbohydrates(P = 0.000) was found in CD group, while the metabolism of lipids was increased. In CD patients, three miRNAs were induced in affected mucosa: mir-144(6.2 ± 1.3 fold), mir-519(21.8 ± 3.1) and mir-211(2.3 ± 0.4). CONCLUSION Changes in microbial function in active non-treated CD subjects and three miRNAs in affected vs non-affected mucosa have been found. miRNAs profile may serve as a biomarker. | María Rojas-Feria Teresa Romero-García Jose Angel Fernández Caballero-Rico Helena Pastor Ramírez Marta Avilés-Recio Manuel Castro-Fernandez Natalia Chueca Porcuna Manuel Romero-Gomez Federico García Lourdes Grande JoséA Del Campo | 2018 | World Journal of Gastroenterology2018，24，46： | 7 |
| 2 | Unilateral benign cho anal polyp: review of 51 patients显示文摘 | VIROS PORCUNA D MONTSERRAT GILl J R GRAS CABRERIZ()J R | 2008 | Acta ()torrino laringol Esp2008，59，： | 1 |
| 3 | Pólipo coanal unilateral benigno: revisión de 51 pacientes显示文摘 | David Virós Porcuna Joan R. Montserrat Gili Juan R. Gras Cabrerizo Montse López Vilas Albert Pujol Olmo | 2008 | Acta Otorrinolaringologica Espanola2008，，2： | 1 |
| 4 | Peotoralis major flaps,Evolution of their use in the age of mfcrovascufarized flaps显示文摘 | Viros Porcuna O Leon Vintro X Lopez Vilas M | 2008 | Acta Otorrinolaringol Esp2008，59，6： | 1 |
| 5 | Actualización en la terapia de la hepatitis C. Nuevos fármacos, monitorización de la respuesta y resistencias显示文摘 | Natalia Chueca Porcuna Marta álvarez Estévez Jorge Parra Ruiz José Hernández Quero Federico García García | 2013 | Enfermedades Infecciosas y Microbiologia Clinica2013，，： | 1 |
| 6 | Review of 51 patients 显示文摘 | DAVID VIROS PORCUNA MONTSERRAT GILI UNILATERAL BENIGN CHOANAL POLYP | 2008 | Acta Otreeinolaringol Esp2008，59，2： | 1 |
| 7 | Combined endoscopic treatment for Zenker's diverticulum versus open approach,review of our experience显示文摘 | Virós Porcuna D Zarraonandia Andraca Ⅰ León Vintró X | 2009 | Acta Otorrinolaringol Esp2009，60，6： | 1 |
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