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| 1 | Multiplexed Biosensing Diagnostic Platforms Detecting Autoantibodies to Tumor-Associated Antigens from Exosomes Released by CRC Cells and Tissue Samples Showed High Diagnostic Ability for Colorectal Cancer显示文摘Colorectal cancer(CRC)is the second leading cause of cancer-related death worldwide.The five-year survival rate of CRC patients depends on the stage at diagnosis,being higher than 80%when CRC is diagnosed in the early stages but lower than 10%when CRC is diagnosed in advanced stages.Autoantibodies against specific CRC autoantigens(tumor-associated antigens(TAAs))in the sera of patients have been widely demonstrated to aid in early diagnosis.Thus,we herein aim to identify autoantigens target of autoantibodies specific to CRC that possess a significant ability to discriminate between CRC patients and healthy individuals by means of liquid biopsy.To that end,we examined the protein content of the exosomes released by five CRC cell lines and tissue samples from CRC patients by means of immunoprecipitation coupled with mass spectrometry analysis.A total of 103 proteins were identified as potential autoantigens specific to CRC.After bioinformatics and meta-analysis,we selected 15 proteins that are more likely to be actual CRC autoantigens in order to evaluate their role in CRC prognosis by Western blot(WB)and immunohistochemistry(IHC).We found dysregulation at the protein level for 11 of these proteins in both tissue and plasma exosome samples from patients,along with an association of nine of these proteins with CRC prognosis.After validation,all but one showed a statistically significant high diagnostic ability to distinguish CRC patients and individuals with premalignant lesions from healthy individuals,either by luminescence Halotag-based beads,or by a multiplexed biosensing platform involving the use of magnetic microcarriers as solid support modified with covalently immobilized Halotag fusion proteins constructed for CRC detection.Taken together,our results highlight the usefulness of the approach defined here to identify the TAAs specific to chronic diseases;they also demonstrate that the measurement of autoantibody levels in plasma against the TAAs identified here could be integrated into a point-of-care(POC)device for CRC detection with high diagnostic ability. | Ana Montero-Calle Itziar Aranguren-Abeigon María Garranzo-Asensio Carmen Poves María Jesús Fernández-Aceñero Javier Martínez-Useros Rodrigo Sanz Jana Dziaková Javier Rodriguez-Cobos Guillermo Solís-Fernández Eloy Povedano Maria Gamella Rebeca Magnolia Torrente-Rodríguez Miren Alonso-Navarro Vivian de los Ríos J.Ignacio Casal Gemma Domínguez Ana Guzman-Aranguez Alberto Peláez-García JoséManuel Pingarrón Susana Campuzano Rodrigo Barderas | 2021 | Engineering2021，7，10： | 1 |
| 2 | Ectopic ACTH syndrome caused by pulmonary carcinoid tumourlets显示文摘 | Povedano S T Pastor C V Seoane C P | 2001 | Clin Endocrinol2001，54，6： | 1 |
| 3 | Endoscopic treatment of central airway stenosis: five years' experience显示文摘 | Cosano Povedano A Mufioz Cabrera L Cosano Povedano F J | 2005 | Arch Bronconeumol2005，41，6： | 1 |
| 4 | Efficacy and safety monotherapy dulaglutide of versus metformin in type 2 diabetes in a randomized controlled trial (AWARD-3)显示文摘 | Umpierrez G Tofe Povedano S Prrez Manghi F | 2014 | Diabetes Care2014，37，8： | 1 |
| 5 | Endoscopic treat- ment of central airway stenosis : five years' experience 显示文摘 | Povedano AC Cabrera LM Povedano FJC | 2005 | Arch Bronconeumo12005，41，6： | 1 |
| 6 | Endoscopic treatment of central airway stenosis:five years' experience显示文摘 | Povedano AC Cabrera LM Povedano FJC | 2005 | Arch Bronconeumol2005，41，6： | 1 |
| 7 | Endoscopic Treatment of Central Airway Stenosis:Five Years' experience显示文摘 | COSANO POVEDANO A MUNOZ CABRERA L COSANO POVEDANO FJ | 2005 | Arch Bronconcumol2005，41，6： | 1 |
| 8 | Very early electrodiagnostic findings in Guillain-Barre' syn drome 显示文摘 | Monica Povedano Jordi Montero Carlos Casasnovas | 2011 | Journal of Peripheral Nervous System2011，16，136： | 1 |
| 9 | Survival in amyotrophic lateral sclerosis with home mechanical ventilation:the impact of systematic respiratory assessment and bulbar involvement显示文摘 | Farrero E Prats E Povedano M | 2005 | Chest2005，127，6： | 1 |
| 10 | Stimulated singlefiber EMG of the frontalis and orbicularis oculi muscles in ocular myasthenia gravis显示文摘 | Valls-Canals J Povedano M Montero J | 2003 | Muscle Nerve2003，28，4： | 1 |
| 11 | Endoscopic Treatment of Central Airway Stenosis: Five Years’ Experience显示文摘 | A. Cosano Povedano L. Mu?oz Cabrera F.J. Cosano Povedano J. Rubio Sánchez N. Pascual Martínez A. Escribano Due?as | 2005 | Archivos de Bronconeumología (Internet)2005，，6： | 1 |
| 12 | Efficacy and safety of dulaglutide monotherapy versus met- formin in type 2 diabetes in a randomized controlled trial (A- WARD-3)显示文摘 | UMPIERREZ G TOFE POVEDANO S PEREZ MANGHI F | 2014 | Diabetes Care2014，37，8： | 1 |
| 13 | Once-weekly dulaglutide versus once-daily liraglutide in metformin-treated pa- tients with type 2 diabetes (AWARD-6) : a randomised, open- label, phase 3, non-inferiorlty trial 显示文摘 | DUNGAN KM POVEDANO ST FORST T | 2014 | Lancet2014，384，9951： | 1 |
| 14 | Endoscopic treatment of central airway stenosis:five years'experience显示文摘 | COSANO P A MUNOZ C L COSAN0 POVEDANO F J | 2005 | Arch Bronconeumol2005，41，： | 1 |
| 15 | Once-weekly dulaglutide versus once-daily liraglutide in metformin-treated patients with type 2diabetes(AWARD-6):a randomised,open-label,phase 3,non-infer iority trial显示文摘 | Dungan KM Povedano ST Forst T | 2014 | Lancet2014，384，9951： | 1 |
| 16 | Survival in amyotrophic lateral sclerosis with home mechanical ventilation:the impact of systematic respiratory assessment and bulbar involvement显示文摘 | Farrero E Prats E Povedano M | 2005 | Chest2005，127，6： | 1 |
| 17 | Endoscopic treatment of central airway stenosis:five years' experience显示文摘 | Cosano Povedano A Mu(n)oz Cabrera L Cosano Povedano F J | 2005 | Archivos de Bronconeumologia2005，41，： | 1 |
| 18 | Managementstrategies in posterior circulation intracranial atheroscle-rotic disease 显示文摘 | Povedano G Zuberbuhler P Lylyk P | 2010 | J Endovasc Ther2010，17，3： | 1 |
| 19 | Efficacy and safety of dulaglutide monotherapy versus metformin in type 2 diabetes in a randomized controlled trial (AWARD-3) 显示文摘 | UMPIERREZ G POVEDANO ST MANGHI FP | 2014 | Dia- betes Care2014，37，8： | 1 |
| 20 | Endoscopic treatment of central airway stenosis:five years'experience显示文摘 | COSANO POVEDANO A MUNOZ CABRERA L COSANO POVEDANO F J | 2005 | Arch Bronconeumol2005，41，： | 1 |
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