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| 1 | Prunus genetics and applications after de novo genome sequencing:achievements and prospects显示文摘Prior to the availability of whole-genome sequences,our understanding of the structural and functional aspects of Prunus tree genomes was limited mostly to molecular genetic mapping of important traits and development of EST resources.With public release of the peach genome and others that followed,significant advances in our knowledge of Prunus genomes and the genetic underpinnings of important traits ensued.In this review,we highlight key achievements in Prunus genetics and breeding driven by the availability of these whole-genome sequences.Within the structural and evolutionary contexts,we summarize:(1)the current status of Prunus whole-genome sequences;(2)preliminary and ongoing work on the sequence structure and diversity of the genomes;(3)the analyses of Prunus genome evolution driven by natural and man-made selection;and(4)provide insight into haploblocking genomes as a means to define genome-scale patterns of evolution that can be leveraged for trait selection in pedigree-based Prunus tree breeding programs worldwide.Functionally,we summarize recent and ongoing work that leverages whole-genome sequences to identify and characterize genes controlling 22 agronomically important Prunus traits.These include phenology,fruit quality,allergens,disease resistance,tree architecture,and self-incompatibility.Translationally,we explore the application of sequence-based marker-assisted breeding technologies and other sequence-guided biotechnological approaches for Prunus crop improvement.Finally,we present the current status of publically available Prunus genomics and genetics data housed mainly in the Genome Database for Rosaceae(GDR)and its updated functionalities for future bioinformatics-based Prunus genetics and genomics inquiry. | Maria JoséAranzana Véronique Decroocq Elisabeth Dirlewanger Iban Eduardo Zhong Shan Gao Ksenija Gasic Amy Iezzoni Sook Jung Cameron Peace Humberto Prieto Ryutaro Tao Ignazio Verde Albert G.Abbott Pere Arús | 2019 | Horticulture Research2019，6，1： | 8 |
| 2 | Interplay between post-translational cyclooxygenase-2 modifications and the metabolic and proteomic profile in a colorectal cancer cohort显示文摘BACKGROUND Colorectal cancer(CRC) is the second most common cause of cancer death worldwide. It is broadly described that cyclooxygenase-2(COX-2) is mainly overexpressed in CRC but less is known regarding post-translational modifications of this enzyme that may regulate its activity, intracellular localization and stability. Since metabolic and proteomic profile analysis is essential for cancer prognosis and diagnosis, our hypothesis is that the analysis of correlations between these specific parameters and COX-2 state in tumors of a high number of CRC patients could be useful for the understanding of the basis of this cancer in humans.AIM To analyze COX-2 regulation in colorectal cancer and to perform a detailed analysis of their metabolic and proteomic profile.METHODS Biopsies from both healthy and pathological colorectal tissues were taken under informed consent from patients during standard colonoscopy procedure in the University Hospital of Bellvitge(Barcelona, Spain) and Germans Trias i Pujol University Hospital(Campus Can Ruti)(Barcelona, Spain). Western blot analysis was used to determine COX-2 levels. Deglycosylation assays were performed in both cells and tumor samples incubating each sample with peptide N-glycosidase F(PNGase F). Prostaglandin E2(PGE2) levels were determined using a specific ELISA. 1 H high resolution magic angle spinning(HRMAS) analysis was performed using a Bruker AVIII 500 MHz spectrometer and proteomic analysis was performed in a nano-liquid chromatography-tandem mass spectrometer(nano LC-MS/MS) using a QExactive HF orbitrap MS.RESULTS Our data show that COX-2 has a differential expression profile in tumor tissue of CRC patients vs the adjacent non-tumor area, which correspond to a glycosylated and less active state of the protein. This fact was associated to a lesser PGE2 production in tumors. These results were corroborated in vitro performing deglycosylation assays in HT29 cell line where COX-2 protein profile was modified after PNGase F incubation, showing higher PGE2 levels. Moreover,HRMAS analysis indicated that tumor tissue has altered metabolic features vs non-tumor counterparts, presenting increased levels of certain metabolites such as taurine and phosphocholine and lower levels of lactate. In proteomic experiments, we detected an enlarged number of proteins in tumors that are mainly implicated in basic biological functions like mitochondrial activity,DNA/RNA processing, vesicular trafficking, metabolism, cytoskeleton and splicing.CONCLUSION In our colorectal cancer cohort, tumor tissue presents a differential COX-2 expression pattern with lower enzymatic activity that can be related to an altered metabolic and proteomic profile. | Patricia Prieto Rafael I Jaén Daniel Calle María Gómez-Serrano Estefanía Nú?ez María Fernández-Velasco Paloma Martín-Sanz Sergio Alonso Jesús Vázquez Sebastián Cerdán Miguel ángel Peinado Lisardo Boscá | 2019 | World Journal of Gastroenterology2019，25，4： | 4 |
| 3 | Effects of dietary protein concentration on postweaning growth of Boer crossbred and Spanish goat wethers显示文摘 | PRIETO I GOETSCH A L BONSKALIEVA V | 2000 | Journal of Animal Science2000，78，9： | 1 |
| 4 | The phytotoxic activity of some metabolites of Botrytis cinerea 显示文摘 | Rebordinos L Cantoral J M Prieto M V | 1996 | Phytochemistry1996，42，： | 1 |
| 5 | Axonal plasticity and functional recovery after spinal cord injury in mice deficient in both glial fibrillary acidic protein and vimentin genes显示文摘 | Menet V Prieto M Privat A | 2003 | Proc Natl Acad Sci USA2003，100，15： | 1 |
| 6 | Loss of Mda-7 expression with progression of melanoma显示文摘 | Ellerhorst J A Prieto V G Ekmekcioglu S | 2002 | J Clin Oncol2002，20，4： | 1 |
| 7 | A randomized phase Ⅱ study of cilengitide (EMD 121974) in patients with metastatic melanoma显示文摘 | Kim KB Prieto V Joseph RW | 2012 | Melanoma Res2012，22，4： | 1 |
| 8 | Detection and quantification of functional genes of cellulose-degrading, fer- mentative, and sulfate-reducing bacteria and methanogenic ar- chaea显示文摘 | Pereyra L P Hiibel S R Prieto Riquelme M V | 2010 | Applied and Environmental Microbiology2010，76，7： | 1 |
| 9 | Effects of Intense Pulsed Light on Sundamaged Human Skin, Routine, and Ultra Structural Analysis 显示文摘 | PRIETO V G SADICK N S LLORETA J | 2002 | Lasers Surg Med2002，30，： | 1 |
| 10 | Axonal plasticity and functionalrecovery after spinal cord injury in mice deficient in both glial fibrillaryacidic protein and vimentin genes 显示文摘 | Menet V Prieto M Privat A | 2003 | Proc Natl Acad Sci USA2003，100，15： | 1 |
| 11 | Inci- dence and distribution of argininosuccinate synthetase de- ficiency in human eaneers:a method for identifying canc- ers sensitive to arginine deprivation 显示文摘 | DILLON B J PRIETO V G CURLEY S A | 2004 | Cancer2004，100，4： | 1 |
| 12 | Synergistic effect in the hydroxylation of phenol over CoNiA1 ternary hydrotaleites 显示文摘 | Rives V Prieto O Dubey A | 2003 | J Catal 20032003，，： | 1 |
| 13 | Lithium battery mate- rials LiMPO4 (M : Mn, Fe, Co, and Ni):insights into defect as- sociation, transport mechanisms, and doping behavior 显示文摘 | Fisher C A Hart Prieto V M Islam M S | 2008 | Chemistry of Materials2008，20，59： | 1 |
| 14 | The role of adjuvant ra- diotherapy in the local management of desmoplastic melanoma显示文摘 | Guadagnolo BA Prieto V Weber R | 2014 | Cancer2014，120，9： | 1 |
| 15 | Determination of ciprofloxacin and enoxacin in human serum samples by micellar liquid chromatography显示文摘 | V'ILCHEZ JL ARAUJO L PRIETO A | 2004 | Analytica Chimica Acta2004，516，12： | 1 |
| 16 | The phytotoxic activity of some metalolites of Botrytis cinerea显示文摘 | Rebordinos L Cantoral J M Prieto M V | 1996 | Phytochemistry1996，42，： | 1 |
| 17 | Relationship between induced sputum cell counts and fluid-phase eosinophil cationic protein and clinical or physiologic profiles in mild asthma 显示文摘 | Gutierrez V Prieto L Tones V | 1999 | Ann Allergy Asthma Immunol1999，82，2： | 1 |
| 18 | Probing extra dimensions using a superconductiong accelerometer 显示文摘 | PAIK H J PRIETO V MOODY M V | 2004 | Journal of the Korean Physical Society2004，45，1： | 1 |
| 19 | Dislocation arthroplasty of the shoulder显示文摘 | SAMILSON RL PRIETO V | 1983 | J Bone Joint Surg Ant1983，65，： | 1 |
| 20 | Reduction of speckle noise in digital holography by using digital image processing 显示文摘 | Garcia-Sucerquia J Ramirez J A H Prieto D V | 2005 | Optik-International Journal for Light and Electron Optics2005，116，1： | 1 |
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